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Ted Pella microtome
Microtome, supplied by Ted Pella, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Ted Pella
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
93/100 stars

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Centrifugation:

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM). .. Cells were isolated by centrifugation (100× g for 2 min), resuspended in PBS containing 0.1% bovine serum albumin (0.1% BSA/PBS), and passed through filter-top tubes (35 µm, Falcon).

Transferring:

Article Title: Behavioral analysis of NR2C knockout mouse reveals deficit in working memory and acquisition of conditioned fear
Article Snippet: 300–350 μm thick coronal sections were prepared using vibrating microtome (Vibratome series 1000, Ted Pella, Redding, CA). .. Whole-cell patch recordings were obtained from lateral amygdala (LA) principal neurons in voltage-clamp configuration with an Axopatch 200B (Molecular Devices, CA) and a pipette resistance of 5–10 mOhm.

Article Title: Metabotropic Glutamate Receptors 1 and 5 Differentially Regulate CA1 Pyramidal Cell Function
Article Snippet: The hemisected brain was glued onto the stage of a vibrating microtome (Vibratome series 1000, Ted Pella, Redding, CA), and sections of 300 μm thickness were cut and stored in an incubation chamber at room temperature for ∼1 hr before use. .. Conventional blind and visually guided whole-cell patch recordings were obtained from CA1 pyramidal neurons both in voltage-clamp and in current-clamp configuration with an Axopatch 200A (Axon Instruments, Foster City, CA) and a pipette resistance of 5–7 MΩ.

Incubation:

Article Title: Tau mediates microtubule bundle architectures mimicking fascicles of microtubules found in the axon initial segment
Article Snippet: Samples were embedded in resin, then embedded in spur plastic and incubated overnight, with resin poured into flat embedding moulds and held at 65 °C for 48 h and cooled overnight. .. Plastic-embedded samples were then cut to ≈50-nm slices with a microtome (Ted Pella, Redding, CA) and transferred to highly stable Formvar carbon-coated copper EM grids (Ted Pella, Redding, CA).

Article Title: Evolution of Diversity in Spatially Structured Escherichia coli Populations
Article Snippet: To entrap the biofilm, the flow cell was incubated for 2 h at room temperature and then the growth chamber was disassembled. .. After the agar hardened, 100-μm sections were prepared using a microtome (Vibratome 3000; Ted Pella, Redding, CA).

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: CUBIC technique The brain was dissected out and exposed to 4% paraformaldehyde at 4 °C for 24 h. Coronal sections (thickness of 400 μm) were prepared with the use of a vibrating microtome (LinearSlicer PRO7, Tedpella), washed in PBS, and then subjected to the CUBIC (clear, unobstructed brain/body imaging cocktails) procedure as described previously . .. In brief, the sections were incubated at room temperature first with 50% CUBIC1 reagent (25% urea, 25% Quadrol, and 15% Triton X-100) for 6 h and then with 100% CUBIC1 for 6 h. They were then washed three times in PBS for 30–60 min each time before incubation at room temperature first with 50% CUBIC2 reagent (25% urea, 50% sucrose, and 10% triethanolamine in PBS) for 6–12 h and then with Hoechst 33342 (1:1000) in 100% CUBIC2 for 24–48 h.

Article Title: Metabotropic Glutamate Receptors 1 and 5 Differentially Regulate CA1 Pyramidal Cell Function
Article Snippet: .. The hemisected brain was glued onto the stage of a vibrating microtome (Vibratome series 1000, Ted Pella, Redding, CA), and sections of 300 μm thickness were cut and stored in an incubation chamber at room temperature for ∼1 hr before use. ..

Activity Assay:

Article Title: A POPULATION OF MITOCHONDRION-RICH CELLS IN PARS RECTA OF MOUSE KIDNEY
Article Snippet: Mitochondrial activity that generates reactive oxygen species can be detected in renal tissue by the administration through the vasculature of nitroblue tetrazolium (NBT; ). .. For the preparation of semithin and ultrathin plastic sections, glutaraldehyde-perfused kidneys were cut into 50 μm sections with a vibrating microtome (D.S.K Microslicer DTK-3000, Ted Pella, Redding CA).

Modification:

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: .. In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM). .. Regions of the sections corresponding approximately to cortical layers II/III–IV (upper layers) and to layers V and VI (deep layers) were dissected out under visual guidance and transillumination with a microscope (Zeiss Stemi 2000-C) and were also collected separately into ice-cold DMEM.

Electron Microscopy:

Article Title: Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions
Article Snippet: Paragraph title: Electron microscopy ... 250–300 contiguous sections were cut using a microtome (UC6; Leica) from each condition and collected onto formvar–coated (#RT15820; EMS) grids (#1GC12H; Ted Pella).

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy. .. Brains were cut in 40 μm coronal sections, and appropriate ILmPFC or LC sections were selected for IHC processing using the rat brain atlas of Paxinos and Watson (Paxinos, 1986).

Flow Cytometry:

Article Title: Evolution of Diversity in Spatially Structured Escherichia coli Populations
Article Snippet: To entrap the biofilm, the flow cell was incubated for 2 h at room temperature and then the growth chamber was disassembled. .. After the agar hardened, 100-μm sections were prepared using a microtome (Vibratome 3000; Ted Pella, Redding, CA).

Dissection:

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: Paragraph title: Tissue dissection, dissociation, and FACS ... In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM).

Imaging:

Article Title: Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions
Article Snippet: 250–300 contiguous sections were cut using a microtome (UC6; Leica) from each condition and collected onto formvar–coated (#RT15820; EMS) grids (#1GC12H; Ted Pella). .. The sections were stained with 2.5% uranyl acetate in 70% methanol for four minutes prior to imaging.

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: .. CUBIC technique The brain was dissected out and exposed to 4% paraformaldehyde at 4 °C for 24 h. Coronal sections (thickness of 400 μm) were prepared with the use of a vibrating microtome (LinearSlicer PRO7, Tedpella), washed in PBS, and then subjected to the CUBIC (clear, unobstructed brain/body imaging cocktails) procedure as described previously . .. In brief, the sections were incubated at room temperature first with 50% CUBIC1 reagent (25% urea, 25% Quadrol, and 15% Triton X-100) for 6 h and then with 100% CUBIC1 for 6 h. They were then washed three times in PBS for 30–60 min each time before incubation at room temperature first with 50% CUBIC2 reagent (25% urea, 50% sucrose, and 10% triethanolamine in PBS) for 6–12 h and then with Hoechst 33342 (1:1000) in 100% CUBIC2 for 24–48 h.

Transmission Assay:

Article Title: Tau mediates microtubule bundle architectures mimicking fascicles of microtubules found in the axon initial segment
Article Snippet: Plastic-embedded samples were then cut to ≈50-nm slices with a microtome (Ted Pella, Redding, CA) and transferred to highly stable Formvar carbon-coated copper EM grids (Ted Pella, Redding, CA). .. Data were taken using the JEOL 1230 Transmission Electron Microscope.

Injection:

Article Title: Removal of Polysialic Acid Triggers Dispersion of Subventricularly Derived Neuroblasts into SurroundingCNS Tissues
Article Snippet: Ten days after the intraventricular injection, animals were injected intraperitoneally with BrdU (Sigma-Aldrich; 50 mg/kg body weight dissolved in 0.1N NaOH, 0.9% NaCl) and killed at different time points (4 h, 3 d, and 10 d from the BrdU injection). .. Brains were sliced in a vibrating microtome (Pelco-101, Ted Pella) in 40-μm-thick sagittal sections.

Immunofluorescence:

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: For light and immunofluorescence microscopy, the same procedure was conducted with 4% formaldehyde in 0.1 M PB without acrolein (n=6; 3 male, 3 female). .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy.

Transmission Electron Microscopy:

Article Title: Tau mediates microtubule bundle architectures mimicking fascicles of microtubules found in the axon initial segment
Article Snippet: Paragraph title: Plastic-embedded TEM sample preparation and TEM ... Plastic-embedded samples were then cut to ≈50-nm slices with a microtome (Ted Pella, Redding, CA) and transferred to highly stable Formvar carbon-coated copper EM grids (Ted Pella, Redding, CA).

Fluorescence:

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy. .. Brains were cut in 40 μm coronal sections, and appropriate ILmPFC or LC sections were selected for IHC processing using the rat brain atlas of Paxinos and Watson (Paxinos, 1986).

Isolation:

Article Title: ATP sensitivity of preB?tzinger complex neurones in neonatal rat in vitro: mechanism underlying a P2 receptor-mediated increase in inspiratory frequency
Article Snippet: The brainstem–spinal cord was then isolated in cold, artificial cerebrospinal fluid (aCSF) containing (m m ): 120 NaCl, 3 KCl, 1.0 CaCl2 , 2.0 MgSO4 , 26 NaHCO3 , 1.25 NaH2 PO4 , 20 d -glucose, equilibrated with 95% O2 –5% CO2 . .. The brainstem–spinal cord was pinned to a wax chuck and serial 100–200 μm sections were cut in the rostral to caudal direction using a vibrating microtome (Pelco-101, Ted Pella, CA, USA, or Leica VT1000S, Nussloch, Germany) and trans-illuminated to identify anatomical landmarks.

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM). .. Cells were isolated by centrifugation (100× g for 2 min), resuspended in PBS containing 0.1% bovine serum albumin (0.1% BSA/PBS), and passed through filter-top tubes (35 µm, Falcon).

Immuno-Electron Microscopy:

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: Sections from the acrolein-fixed cases (n=6; 3 male, 3 female) were used for immunoelectron microscopy, as acrolein fixation yields optimal tissue preservation for ultrastructural analysis. .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy.

Immunohistochemistry:

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: Male and female rats used for immunohistochemistry (IHC) were deeply anesthetized using isoflurane (Vedco, St. Joseph, MO) and subsequently transcardially perfused through the ascending aorta with the following fixatives: 10 ml of 1000 units/ml heparinized saline, 50 ml of 3.75% acrolein in 2% formaldehyde and 200 ml of 2% formaldehyde in 0.1 M phosphate buffer (PB) pH 7.4. .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy.

Microscopy:

Article Title: Tau mediates microtubule bundle architectures mimicking fascicles of microtubules found in the axon initial segment
Article Snippet: Plastic-embedded samples were then cut to ≈50-nm slices with a microtome (Ted Pella, Redding, CA) and transferred to highly stable Formvar carbon-coated copper EM grids (Ted Pella, Redding, CA). .. Data were taken using the JEOL 1230 Transmission Electron Microscope.

Article Title: A POPULATION OF MITOCHONDRION-RICH CELLS IN PARS RECTA OF MOUSE KIDNEY
Article Snippet: For the preparation of semithin and ultrathin plastic sections, glutaraldehyde-perfused kidneys were cut into 50 μm sections with a vibrating microtome (D.S.K Microslicer DTK-3000, Ted Pella, Redding CA). .. These sections were examined in fresh-cut form to confirm NBT staining, and selected sections containing diformazan deposits were processed either with or without osmication, then dehydrated and infiltrated with Poly/Bed 812 resin (Polybed, Inc., Warrington, PA), after which they were affixed to microscope slides and cured at 60°C as previously described ( ); regions were then selected for cutting on Sorvall MT-2B or Leica Ultracut EM UC7 ultramicrotomes.

Article Title: Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions
Article Snippet: 250–300 contiguous sections were cut using a microtome (UC6; Leica) from each condition and collected onto formvar–coated (#RT15820; EMS) grids (#1GC12H; Ted Pella). .. Sections were imaged on a Hitachi H-7100 electron microscope equipped with a Gatan digital camera (Gatan, Orius, Pleasanton, CA).

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM). .. Regions of the sections corresponding approximately to cortical layers II/III–IV (upper layers) and to layers V and VI (deep layers) were dissected out under visual guidance and transillumination with a microscope (Zeiss Stemi 2000-C) and were also collected separately into ice-cold DMEM.

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy. .. Brains were cut in 40 μm coronal sections, and appropriate ILmPFC or LC sections were selected for IHC processing using the rat brain atlas of Paxinos and Watson (Paxinos, 1986).

Mouse Assay:

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: Young adult Aldh1l1-eGFP mice were killed by cervical dislocation, and layer dissection was performed as described . .. In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM).

Article Title: Behavioral analysis of NR2C knockout mouse reveals deficit in working memory and acquisition of conditioned fear
Article Snippet: Briefly, after isoflurane anesthesia mice were decapitated and brains were removed rapidly and placed in ice-cold artificial cerebrospinal fluid (ACSF) of the following composition (in mM): 130 NaCl, 24 NaHCO3 , 3.5 KCl, 1.25 NaH2 PO4 , 0.5 CaCl2 , 3 MgCl2 and 10 glucose saturated with 95% O2 /5% CO2 . .. 300–350 μm thick coronal sections were prepared using vibrating microtome (Vibratome series 1000, Ted Pella, Redding, CA).

Labeling:

Article Title: Removal of Polysialic Acid Triggers Dispersion of Subventricularly Derived Neuroblasts into SurroundingCNS Tissues
Article Snippet: Paragraph title: EndoN treatment and BrdU labeling. ... Brains were sliced in a vibrating microtome (Pelco-101, Ted Pella) in 40-μm-thick sagittal sections.

FACS:

Article Title: Layer-specific morphological and molecular differences in neocortical astrocytes and their dependence on neuronal layers
Article Snippet: Paragraph title: Tissue dissection, dissociation, and FACS ... In brief, the brain was embedded in 4% low-melting point agarose (Lonza), and coronal sections were prepared at a thickness of 250 μm with a vibrating microtome (LinearSlicer PRO7, Tedpella) and collected in ice-cold Dulbecco’s modified Eagle’s medium (DMEM).

Sample Prep:

Article Title: Tau mediates microtubule bundle architectures mimicking fascicles of microtubules found in the axon initial segment
Article Snippet: Paragraph title: Plastic-embedded TEM sample preparation and TEM ... Plastic-embedded samples were then cut to ≈50-nm slices with a microtome (Ted Pella, Redding, CA) and transferred to highly stable Formvar carbon-coated copper EM grids (Ted Pella, Redding, CA).

Preserving:

Article Title: Localization of endogenous amyloid-β to the coeruleo-cortical pathway: consequences of noradrenergic depletion
Article Snippet: Sections from the acrolein-fixed cases (n=6; 3 male, 3 female) were used for immunoelectron microscopy, as acrolein fixation yields optimal tissue preservation for ultrastructural analysis. .. The brains were removed, cut into 4–5 mm coronal blocks, stored in 2% formaldehyde fixative for an additional 30 minutes and then sectioned (30–40 um) on a vibrating microtome (Vibratome; Pelco EasiSlicer, Ted Pella, Redding, CA) for electron microscopy, or a cryostat (Microm HM 50, Microm International, Waldorf, Germany) for light and fluorescence microscopy.

Knock-Out:

Article Title: Behavioral analysis of NR2C knockout mouse reveals deficit in working memory and acquisition of conditioned fear
Article Snippet: Acute amygdala slices were obtained from conditioned (as in test 1) and naïve NR2C wildtype and knockout mice (P20 to P25) in accordance with the approved protocols of Creighton University Institutional Animal Care and Use Committee. .. 300–350 μm thick coronal sections were prepared using vibrating microtome (Vibratome series 1000, Ted Pella, Redding, CA).

Slice Preparation:

Article Title: Glycinergic and GABAergic calcium responses in the developing lateral superior olive
Article Snippet: Paragraph title: Animals and slice preparation ... Coronal slices of the brainstem (200–300 μm thick) were cut on a vibrating microtome (DTK-1500E, Ted Pella, Redding, CA, USA).

Staining:

Article Title: Glycinergic and GABAergic calcium responses in the developing lateral superior olive
Article Snippet: ACSF used for preparation, storage and staining of slices also contained 1 mM kynurenic acid. .. Coronal slices of the brainstem (200–300 μm thick) were cut on a vibrating microtome (DTK-1500E, Ted Pella, Redding, CA, USA).

Article Title: A POPULATION OF MITOCHONDRION-RICH CELLS IN PARS RECTA OF MOUSE KIDNEY
Article Snippet: For the preparation of semithin and ultrathin plastic sections, glutaraldehyde-perfused kidneys were cut into 50 μm sections with a vibrating microtome (D.S.K Microslicer DTK-3000, Ted Pella, Redding CA). .. These sections were examined in fresh-cut form to confirm NBT staining, and selected sections containing diformazan deposits were processed either with or without osmication, then dehydrated and infiltrated with Poly/Bed 812 resin (Polybed, Inc., Warrington, PA), after which they were affixed to microscope slides and cured at 60°C as previously described ( ); regions were then selected for cutting on Sorvall MT-2B or Leica Ultracut EM UC7 ultramicrotomes.

Article Title: Ultrafast endocytosis at Caenorhabditis elegans neuromuscular junctions
Article Snippet: 250–300 contiguous sections were cut using a microtome (UC6; Leica) from each condition and collected onto formvar–coated (#RT15820; EMS) grids (#1GC12H; Ted Pella). .. The sections were stained with 2.5% uranyl acetate in 70% methanol for four minutes prior to imaging.

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