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Technical Products International Inc microtome
Microtome, supplied by Technical Products International Inc, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Technical Products International Inc
Average 94 stars, based on 9 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
94/100 stars

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Related Articles

Immunocytochemistry:

Article Title: A Characterization of the Manduca sexta Serotonin Receptors in the Context of Olfactory Neuromodulation
Article Snippet: .. Immunocytochemistry The brains of 2-5 day old moths were dissected (29 total spread across the different treatments described below) from the head capsules under physiological saline and placed in 4% paraformaldehyde overnight at 4o C. Brains were then washed in PBS (pH 6.9) embedded in 5% low melting point agarose (Sigma) and sectioned at 75µM thickness on a vibrating microtome (Technical Products International, St. Louis, MO). .. Sections were then washed in phosphate buffered saline (PBS) with 0.5% Triton-X 100 (PBST), blocked in 2% IgG-free bovine serum albumin (BSA) for one hour and then incubated overnight at 4o C in PBST with 2% BSA and a 1:300 dilution of the AM3A antibody.

Patch Clamp:

Article Title: Nervous System Pathophysiology: Altered A-type potassium channel function in the nucleus tractus solitarii in acquired temporal lobe epilepsy
Article Snippet: Nine to twelve weeks after pilo-SE or vehicle injection, whole cell patch-clamp recordings were performed in GABAergic NTS neurons in brain stem slices taken from GIN mice (13–18 wk of age). .. Transverse (i.e., coronal) slices from the caudal brain stem (300 µm) were made in 0–2°C oxygenated (95% O2 -5% CO2 ) artificial cerebrospinal fluid (ACSF) with a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO) and transferred to a holding chamber.

Article Title: mGluR5 Upregulation Increases Excitability of Hypothalamic Presympathetic Neurons through NMDA Receptor Trafficking in Spontaneously Hypertensive Rats
Article Snippet: A tissue block containing the PVN was trimmed and glued onto the stage of a vibrating microtome (Technical Products International). .. Whole-cell patch-clamp recordings were performed in FluoSphere-labeled PVN neurons in the hypothalamic slices.

Article Title: Functional Neuroplasticity in the Nucleus Tractus Solitarius and Increased Risk of Sudden Death in Mice with Acquired Temporal Lobe Epilepsy
Article Snippet: On-cell and whole-cell patch-clamp recordings were performed in GABAergic NTS neurons from brainstem slices in GIN mice. .. Transverse (coronal) slices (300 μm) from the caudal brainstem were made in cold (0–2°C) oxygenated (95% O2 /5% CO2 ) artificial cerebral spinal fluid (aCSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International) and transferred to a holding chamber.

Isolation:

Article Title: Regionally Localized Recurrent Excitation in the Dentate Gyrus of a Cortical Contusion Model of Posttraumatic Epilepsy
Article Snippet: Brains were blocked and glued to a sectioning stage, and 400-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO). .. The hippocampus was isolated from all surrounding tissue, making sure to completely remove the entorhinal cortex.

Article Title: Brain Injury-Induced Synaptic Reorganization in Hilar Inhibitory Neurons Is Differentially Suppressed by Rapamycin
Article Snippet: Brains were blocked, glued to a sectioning stage, and 350-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International). .. The hippocampus was isolated from surrounding tissue, making sure to completely remove the entorhinal cortex, thereby avoiding synaptic excitation of hilar inhibitory interneurons by activity of intact cortical neurons mediating performant path input.

Article Title: Posttraumatic epilepsy after controlled cortical impact injury in mice
Article Snippet: Brains were blocked, glued to a sectioning stage, and 400 μm-thick horizontal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO). .. The hippocampus was isolated from surrounding tissue, making sure to completely remove the entorhinal cortex.

Microscopy:

Article Title: Selective Enhancement of Synaptic Inhibition by Hypocretin (Orexin) in Rat Vagal Motor Neurons: Implications for Autonomic Regulation
Article Snippet: Transverse brainstem slices (300–400 μ m) containing the caudal vagal complex were made in 0–2°C, oxygenated (95% O2 or 5% CO2 ) artificial CSF (ACSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO). .. In most cases, a single brain slice was then transferred to a submersion-style recording chamber on a fixed stage mounted under an upright (Olympus BX50WI; Olympus Immunochemicals, Melville, NY) or an inverted (Nikon TE200; Nikon, Melville, NY) microscope and continuously perfused with ACSF.

Article Title: Dexamethasone Rapidly Increases GABA Release in the Dorsal Motor Nucleus of the Vagus via Retrograde Messenger-Mediated Enhancement of TRPV1 Activity
Article Snippet: Transverse brainstem slices (350-400 µm) containing the DVC were made using a vibrating microtome (Vibratome Series 1000; Technical Products Intl., St. Louis, MO). .. Slices were transferred to a recording chamber mounted on a fixed stage under an upright microscope (Olympus BX51WI; Melville, NY).

Article Title: Nervous System Pathophysiology: Altered A-type potassium channel function in the nucleus tractus solitarii in acquired temporal lobe epilepsy
Article Snippet: Transverse (i.e., coronal) slices from the caudal brain stem (300 µm) were made in 0–2°C oxygenated (95% O2 -5% CO2 ) artificial cerebrospinal fluid (ACSF) with a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO) and transferred to a holding chamber. .. For recordings, a single brain slice was transferred to a chamber mounted on a fixed stage under an upright microscope (BX51WI; Olympus, Melville, NY), where it was continuously superfused with oxygenated and warmed (30–32°C) ACSF.

Article Title: Functional Neuroplasticity in the Nucleus Tractus Solitarius and Increased Risk of Sudden Death in Mice with Acquired Temporal Lobe Epilepsy
Article Snippet: Transverse (coronal) slices (300 μm) from the caudal brainstem were made in cold (0–2°C) oxygenated (95% O2 /5% CO2 ) artificial cerebral spinal fluid (aCSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International) and transferred to a holding chamber. .. For recordings, a single brain slice was transferred to a chamber mounted on a fixed stage under an upright microscope (BX51WI; Olympus), where it was superperfused with continuously oxygenated and warmed (30°–32°C) aCSF.

Mouse Assay:

Article Title: Regionally Localized Recurrent Excitation in the Dentate Gyrus of a Cortical Contusion Model of Posttraumatic Epilepsy
Article Snippet: Mice were deeply anesthetized by isoflurane inhalation and decapitated. .. Brains were blocked and glued to a sectioning stage, and 400-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO).

Article Title: Brain Injury-Induced Synaptic Reorganization in Hilar Inhibitory Neurons Is Differentially Suppressed by Rapamycin
Article Snippet: Mice were deeply anesthetized by isoflurane inhalation to effect (lack of tail pinch response) and decapitated while anesthetized. .. Brains were blocked, glued to a sectioning stage, and 350-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International).

Article Title: Posttraumatic epilepsy after controlled cortical impact injury in mice
Article Snippet: Mice were deeply anesthetized by isoflurane inhalation and decapitated. .. Brains were blocked, glued to a sectioning stage, and 400 μm-thick horizontal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO).

Article Title: Effects of TRPV1 activation on synaptic excitation in the dentate gyrus of a mouse model of temporal lobe epilepsy
Article Snippet: Mice were anesthetized to effect by halothane inhalation and then decapitated. .. Brains were then blocked and glued to a sectioning stage and sliced in the horizontal plane such that transverse slices (300–400 μm thickness) of the ventral ~2/3 of the hippocampal formation were cut in cold oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products Intl, St Louis, MO, USA).

Article Title: Nervous System Pathophysiology: Altered A-type potassium channel function in the nucleus tractus solitarii in acquired temporal lobe epilepsy
Article Snippet: Mice were anesthetized via isoflurane inhalation and decapitated while anesthetized. .. Transverse (i.e., coronal) slices from the caudal brain stem (300 µm) were made in 0–2°C oxygenated (95% O2 -5% CO2 ) artificial cerebrospinal fluid (ACSF) with a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO) and transferred to a holding chamber.

Article Title: Functional Neuroplasticity in the Nucleus Tractus Solitarius and Increased Risk of Sudden Death in Mice with Acquired Temporal Lobe Epilepsy
Article Snippet: Mice were deeply anesthetized by isofluorane inhalation and then decapitated while anesthetized. .. Transverse (coronal) slices (300 μm) from the caudal brainstem were made in cold (0–2°C) oxygenated (95% O2 /5% CO2 ) artificial cerebral spinal fluid (aCSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International) and transferred to a holding chamber.

Slice Preparation:

Article Title: Regionally Localized Recurrent Excitation in the Dentate Gyrus of a Cortical Contusion Model of Posttraumatic Epilepsy
Article Snippet: Paragraph title: Slice preparation ... Brains were blocked and glued to a sectioning stage, and 400-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO).

Article Title: Simultaneous Quantification of Anandamide and Other Endocannabinoids in Dorsal Vagal Complex of Rat Brainstem by LC-MS
Article Snippet: Paragraph title: Brainstem Slice Preparation ... Transverse brainstem slices (350-400 μm) containing the DVC was made using a vibrating microtome (Vibratome Series 1000; Technical Products Intl., St. Louis, MO, USA).

Article Title: Brain Injury-Induced Synaptic Reorganization in Hilar Inhibitory Neurons Is Differentially Suppressed by Rapamycin
Article Snippet: Paragraph title: Slice preparation ... Brains were blocked, glued to a sectioning stage, and 350-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International).

Article Title: Selective Enhancement of Synaptic Inhibition by Hypocretin (Orexin) in Rat Vagal Motor Neurons: Implications for Autonomic Regulation
Article Snippet: Paragraph title: Slice preparation ... Transverse brainstem slices (300–400 μ m) containing the caudal vagal complex were made in 0–2°C, oxygenated (95% O2 or 5% CO2 ) artificial CSF (ACSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO).

Article Title: Posttraumatic epilepsy after controlled cortical impact injury in mice
Article Snippet: Paragraph title: Slice preparation ... Brains were blocked, glued to a sectioning stage, and 400 μm-thick horizontal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO).

Article Title: Effects of TRPV1 activation on synaptic excitation in the dentate gyrus of a mouse model of temporal lobe epilepsy
Article Snippet: Paragraph title: Slice preparation ... Brains were then blocked and glued to a sectioning stage and sliced in the horizontal plane such that transverse slices (300–400 μm thickness) of the ventral ~2/3 of the hippocampal formation were cut in cold oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products Intl, St Louis, MO, USA).

Article Title: Dexamethasone Rapidly Increases GABA Release in the Dorsal Motor Nucleus of the Vagus via Retrograde Messenger-Mediated Enhancement of TRPV1 Activity
Article Snippet: Paragraph title: Brainstem slice preparation ... Transverse brainstem slices (350-400 µm) containing the DVC were made using a vibrating microtome (Vibratome Series 1000; Technical Products Intl., St. Louis, MO).

Article Title: Nervous System Pathophysiology: Altered A-type potassium channel function in the nucleus tractus solitarii in acquired temporal lobe epilepsy
Article Snippet: Paragraph title: Brain stem slice preparation. ... Transverse (i.e., coronal) slices from the caudal brain stem (300 µm) were made in 0–2°C oxygenated (95% O2 -5% CO2 ) artificial cerebrospinal fluid (ACSF) with a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO) and transferred to a holding chamber.

Article Title: Muscarinic Receptor Subtypes Differentially Control Synaptic Input and Excitability of Cerebellum-Projecting Medial Vestibular Nucleus Neurons
Article Snippet: Paragraph title: Brainstem slice preparation ... A tissue block containing the MVN was glued onto the stage of a vibrating microtome (Technical Products International, St. Louis, MO).

Article Title: Functional Neuroplasticity in the Nucleus Tractus Solitarius and Increased Risk of Sudden Death in Mice with Acquired Temporal Lobe Epilepsy
Article Snippet: Paragraph title: Brainstem slice preparation ... Transverse (coronal) slices (300 μm) from the caudal brainstem were made in cold (0–2°C) oxygenated (95% O2 /5% CO2 ) artificial cerebral spinal fluid (aCSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International) and transferred to a holding chamber.

Incubation:

Article Title: Simultaneous Quantification of Anandamide and Other Endocannabinoids in Dorsal Vagal Complex of Rat Brainstem by LC-MS
Article Snippet: Transverse brainstem slices (350-400 μm) containing the DVC was made using a vibrating microtome (Vibratome Series 1000; Technical Products Intl., St. Louis, MO, USA). .. Time dependent accumulation of eCBs in brain tissue was studied by using slices incubated at 33-35 °C in oxygenated ACSF for 10-60 min.

Article Title: Selective Enhancement of Synaptic Inhibition by Hypocretin (Orexin) in Rat Vagal Motor Neurons: Implications for Autonomic Regulation
Article Snippet: Transverse brainstem slices (300–400 μ m) containing the caudal vagal complex were made in 0–2°C, oxygenated (95% O2 or 5% CO2 ) artificial CSF (ACSF) using a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO). .. Slices were incubated for at least 1 hr in 33–35°C oxygenated ACSF.

Article Title: A Characterization of the Manduca sexta Serotonin Receptors in the Context of Olfactory Neuromodulation
Article Snippet: Immunocytochemistry The brains of 2-5 day old moths were dissected (29 total spread across the different treatments described below) from the head capsules under physiological saline and placed in 4% paraformaldehyde overnight at 4o C. Brains were then washed in PBS (pH 6.9) embedded in 5% low melting point agarose (Sigma) and sectioned at 75µM thickness on a vibrating microtome (Technical Products International, St. Louis, MO). .. Sections were then washed in phosphate buffered saline (PBS) with 0.5% Triton-X 100 (PBST), blocked in 2% IgG-free bovine serum albumin (BSA) for one hour and then incubated overnight at 4o C in PBST with 2% BSA and a 1:300 dilution of the AM3A antibody.

Article Title: Muscarinic Receptor Subtypes Differentially Control Synaptic Input and Excitability of Cerebellum-Projecting Medial Vestibular Nucleus Neurons
Article Snippet: A tissue block containing the MVN was glued onto the stage of a vibrating microtome (Technical Products International, St. Louis, MO). .. Coronal slices (300 μm thick) were cut and then transferred to an incubation chamber containing the artificial cerebrospinal fluid solution continuously gassed with a mixture of 95% O2 and 5% CO2 at 34 °C for at least 1 h before electrophysiological recordings.

Article Title: mGluR5 Upregulation Increases Excitability of Hypothalamic Presympathetic Neurons through NMDA Receptor Trafficking in Spontaneously Hypertensive Rats
Article Snippet: A tissue block containing the PVN was trimmed and glued onto the stage of a vibrating microtome (Technical Products International). .. Coronal slices (300 μm thick) were cut and the slices were transferred to an incubation chamber filled with aCSF continuously gassed with a mixture of 95% O2 and 5% CO2 at 34°C for at least 1 h before the electrophysiological recordings.

Diffusion-based Assay:

Article Title: mGluR5 Upregulation Increases Excitability of Hypothalamic Presympathetic Neurons through NMDA Receptor Trafficking in Spontaneously Hypertensive Rats
Article Snippet: A tissue block containing the PVN was trimmed and glued onto the stage of a vibrating microtome (Technical Products International). .. In addition, the spinal cord was sectioned at the injected level to confirm that the injection and diffusion sites of FluoroSpheres were located in the vicinity of the intermediolateral cell column ( A ).

Activity Assay:

Article Title: Brain Injury-Induced Synaptic Reorganization in Hilar Inhibitory Neurons Is Differentially Suppressed by Rapamycin
Article Snippet: Brains were blocked, glued to a sectioning stage, and 350-μm-thick coronal slices were cut in cold, oxygenated ACSF using a vibrating microtome (Vibratome Series 1000; Technical Products International). .. The hippocampus was isolated from surrounding tissue, making sure to completely remove the entorhinal cortex, thereby avoiding synaptic excitation of hilar inhibitory interneurons by activity of intact cortical neurons mediating performant path input.

Blocking Assay:

Article Title: Muscarinic Receptor Subtypes Differentially Control Synaptic Input and Excitability of Cerebellum-Projecting Medial Vestibular Nucleus Neurons
Article Snippet: .. A tissue block containing the MVN was glued onto the stage of a vibrating microtome (Technical Products International, St. Louis, MO). .. Coronal slices (300 μm thick) were cut and then transferred to an incubation chamber containing the artificial cerebrospinal fluid solution continuously gassed with a mixture of 95% O2 and 5% CO2 at 34 °C for at least 1 h before electrophysiological recordings.

Article Title: mGluR5 Upregulation Increases Excitability of Hypothalamic Presympathetic Neurons through NMDA Receptor Trafficking in Spontaneously Hypertensive Rats
Article Snippet: .. A tissue block containing the PVN was trimmed and glued onto the stage of a vibrating microtome (Technical Products International). .. Coronal slices (300 μm thick) were cut and the slices were transferred to an incubation chamber filled with aCSF continuously gassed with a mixture of 95% O2 and 5% CO2 at 34°C for at least 1 h before the electrophysiological recordings.

Injection:

Article Title: Nervous System Pathophysiology: Altered A-type potassium channel function in the nucleus tractus solitarii in acquired temporal lobe epilepsy
Article Snippet: Nine to twelve weeks after pilo-SE or vehicle injection, whole cell patch-clamp recordings were performed in GABAergic NTS neurons in brain stem slices taken from GIN mice (13–18 wk of age). .. Transverse (i.e., coronal) slices from the caudal brain stem (300 µm) were made in 0–2°C oxygenated (95% O2 -5% CO2 ) artificial cerebrospinal fluid (ACSF) with a vibrating microtome (Vibratome Series 1000; Technical Products International, St. Louis, MO) and transferred to a holding chamber.

Article Title: mGluR5 Upregulation Increases Excitability of Hypothalamic Presympathetic Neurons through NMDA Receptor Trafficking in Spontaneously Hypertensive Rats
Article Snippet: A tissue block containing the PVN was trimmed and glued onto the stage of a vibrating microtome (Technical Products International). .. In addition, the spinal cord was sectioned at the injected level to confirm that the injection and diffusion sites of FluoroSpheres were located in the vicinity of the intermediolateral cell column ( A ).

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    Technical Products International Inc microtome
    Microtome, supplied by Technical Products International Inc, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Technical Products International Inc
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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