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Sakura Finetek microtome
Microtome, supplied by Sakura Finetek, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Sakura Finetek
Average 93 stars, based on 6 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-03
93/100 stars

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Related Articles

Histopathology:

Article Title: Comparative study of pandemic (H1N1) 2009, swine H1N1, and avian H3N2 influenza viral infections in quails
Article Snippet: .. Histopathology Tissue samples collected from quails 3, 5, and 7 DPI were fixed at room temperature in 10% neutral buffered formalin solution for a minimum of 48 h. After fixation, the tissues were immediately embedded in paraffin and cut by microtome (Sakura Finetek, Japan) into 5-µm sections. .. For the histological examination, the sections were stained with hematoxylin and eosin and assigned a score of 0 to 3 based on the degree of inflammation by a veterinary pathologist (R.T.): 0, no lesion; 1, mild inflammation characterized by focal infiltration of inflammatory cells; 2, moderate inflammation characterized by multifocal infiltration of inflammatory cells; 3, severe inflammation characterized by diffuse infiltration of inflammatory cells.

Imaging:

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Individual fluorescent images were acquired using a TCS SP5 confocal microscope and imaging system (Leica Microsystems), and trimmed and overlaid with Photoshop software (Adobe Systems). .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: Mouse Model of Enteropathogenic Escherichia coli Infection
Article Snippet: Tissue sections 5 μm thick were cut by use of a microtome (Sakura Finetek) and stained with hematoxylin and eosin (H & E) or periodic acid-Schiff (PAS) solutions. .. Images were acquired by use of a DMLB microscope equipped with Fluotar objectives (Leica Microsystems Inc., Bannockburn, Ill.) and a Micropublisher 5.0 camera (Q Imaging, Burnaby, British Columbia, Canada).

Immunohistochemistry:

Article Title: Chotosan ameliorates cognitive and emotional deficits in an animal model of type 2 diabetes: possible involvement of cholinergic and VEGF/PDGF mechanisms in the brain
Article Snippet: Paragraph title: Immunohistochemistry ... A series of 5 μm sections were obtained by using a sliding microtome (IVS-410, SAKURA Finetek Japan, Tokyo, Japan).

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Paragraph title: IHC. ... Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
Article Snippet: .. With a microtome (ACCU-Cut SRM200, Sakura Finetek Europe, Alphen aan den Rijn, The Netherlands), buccolingual cross-sections were obtained from the middle portion of the defects and were ground to a final thickness of 4 μ m. Two slides of each specimen were stained with hematoxylin-eosin, and the other two slides were prepared for immunohistochemical analysis for markers of osteopontin (OPN) (Ncl-o-pontin, mouse monoclonal, Leica Biosystems, Newcastle, United Kingdom) by the polymer HRP method according to the manufacturer. .. Histologic and Histomorphometric Analyses Slides were examined by a blinded examiner under a light microscope (Olympus CX21FS, Olympus Corporation, Tokyo, Japan) at a magnification of ×100.

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: Paragraph title: Immunohistochemistry ... The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide.

Avidin-Biotin Assay:

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide. .. Immunohistochemical visualization of FosB was performed on free-floating sections using antibody and avidin-biotin peroxidase methods as previously described , .

Blocking Assay:

Article Title: Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells
Article Snippet: .. The paraffin-embedded tissue block was sectioned at 5-um thickness on a microtome (Accu-Cut, Sakura Finetek Co., Ltd, Japan) and stained with haematoxylin for nuclear staining and eosin for cytoplasmic staining (H & E); stained areas were viewed using an optical microscope with a magnifying power of ×200. .. DPPH radical scavenging activity assay The effect of MSW on the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical was estimated according to the method of Yoshikawa et al. [ ] with a minor modification.

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Sections were incubated with TSA blocking reagent (PerkinElmer), washed, and incubated with primary antibodies at the respective manufacturer’s recommended concentration overnight at 4˚C. .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide. .. Briefly, after blocking endogenous peroxidase and preincubation in 10% normal horse serum, the sections were incubated in primary rabbit polyclonal affinity purified anti-FosB antibody (sc-48, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted 1∶600 in 0.1 M PBS with 0.1% Triton X-100 for 16 hours at room temperature.

Mouse Assay:

Article Title: Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells
Article Snippet: Hepatic morphology Liver was removed from the mice and fixed in a buffer solution of 10% formalin. .. The paraffin-embedded tissue block was sectioned at 5-um thickness on a microtome (Accu-Cut, Sakura Finetek Co., Ltd, Japan) and stained with haematoxylin for nuclear staining and eosin for cytoplasmic staining (H & E); stained areas were viewed using an optical microscope with a magnifying power of ×200.

Article Title: Chotosan ameliorates cognitive and emotional deficits in an animal model of type 2 diabetes: possible involvement of cholinergic and VEGF/PDGF mechanisms in the brain
Article Snippet: Mice were fixed by intracardiac perfusion with 4% paraformaldehyde in phosphate-buffered saline (PBS) under anesthesia. .. A series of 5 μm sections were obtained by using a sliding microtome (IVS-410, SAKURA Finetek Japan, Tokyo, Japan).

Article Title: Mouse Model of Enteropathogenic Escherichia coli Infection
Article Snippet: For histological analysis, colon tissues of control and EPEC-infected mice were washed with PBS, fixed in 10% neutral buffered formalin, processed by use of a Tissue-Tek VIP 5 processor (Sakura Finetek, Torrance, Calif.), and embedded in paraffin. .. Tissue sections 5 μm thick were cut by use of a microtome (Sakura Finetek) and stained with hematoxylin and eosin (H & E) or periodic acid-Schiff (PAS) solutions.

Concentration Assay:

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Sections were incubated with TSA blocking reagent (PerkinElmer), washed, and incubated with primary antibodies at the respective manufacturer’s recommended concentration overnight at 4˚C. .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Incubation:

Article Title: Chotosan ameliorates cognitive and emotional deficits in an animal model of type 2 diabetes: possible involvement of cholinergic and VEGF/PDGF mechanisms in the brain
Article Snippet: A series of 5 μm sections were obtained by using a sliding microtome (IVS-410, SAKURA Finetek Japan, Tokyo, Japan). .. Washed with Tris-buffered saline (TBS), the specimens were incubated in a microwave oven (250 W, 4 s on and 3 s off, MI-77, Azumaya, Tokyo, Japan) in target retrieval solution (Dako, Denmark) for 15 minutes and then washed with distilled water and TBS.

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Sections were incubated with TSA blocking reagent (PerkinElmer), washed, and incubated with primary antibodies at the respective manufacturer’s recommended concentration overnight at 4˚C. .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide. .. Briefly, after blocking endogenous peroxidase and preincubation in 10% normal horse serum, the sections were incubated in primary rabbit polyclonal affinity purified anti-FosB antibody (sc-48, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted 1∶600 in 0.1 M PBS with 0.1% Triton X-100 for 16 hours at room temperature.

Formalin-fixed Paraffin-Embedded:

Article Title: A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Article Snippet: .. Five-micron sections of the formalin-fixed, paraffin-embedded tissue blocks were cut with a microtome (Accu-Cut SRM 200; Sakura Finetek USA, Inc., Torrance, CA, USA) and attached to microscope slides by heating in an oven for 2 hours at 60°C. ..

Microscopy:

Article Title: A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Article Snippet: .. Five-micron sections of the formalin-fixed, paraffin-embedded tissue blocks were cut with a microtome (Accu-Cut SRM 200; Sakura Finetek USA, Inc., Torrance, CA, USA) and attached to microscope slides by heating in an oven for 2 hours at 60°C. ..

Article Title: Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells
Article Snippet: .. The paraffin-embedded tissue block was sectioned at 5-um thickness on a microtome (Accu-Cut, Sakura Finetek Co., Ltd, Japan) and stained with haematoxylin for nuclear staining and eosin for cytoplasmic staining (H & E); stained areas were viewed using an optical microscope with a magnifying power of ×200. .. DPPH radical scavenging activity assay The effect of MSW on the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical was estimated according to the method of Yoshikawa et al. [ ] with a minor modification.

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Individual fluorescent images were acquired using a TCS SP5 confocal microscope and imaging system (Leica Microsystems), and trimmed and overlaid with Photoshop software (Adobe Systems). .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: Mouse Model of Enteropathogenic Escherichia coli Infection
Article Snippet: Tissue sections 5 μm thick were cut by use of a microtome (Sakura Finetek) and stained with hematoxylin and eosin (H & E) or periodic acid-Schiff (PAS) solutions. .. Images were acquired by use of a DMLB microscope equipped with Fluotar objectives (Leica Microsystems Inc., Bannockburn, Ill.) and a Micropublisher 5.0 camera (Q Imaging, Burnaby, British Columbia, Canada).

Expressing:

Article Title: Chotosan ameliorates cognitive and emotional deficits in an animal model of type 2 diabetes: possible involvement of cholinergic and VEGF/PDGF mechanisms in the brain
Article Snippet: Immunohistochemistry CTS administration-induced changes in expression levels of ChAT in the medial septum and VEGF in the retia of m/m and db/db mice were also examined by immunohistochemical analysis [ ]. .. A series of 5 μm sections were obtained by using a sliding microtome (IVS-410, SAKURA Finetek Japan, Tokyo, Japan).

Staining:

Article Title: Desalinated underground seawater of Jeju Island (Korea) improves lipid metabolism in mice fed diets containing high fat and increases antioxidant potential in t-BHP treated HepG2 cells
Article Snippet: .. The paraffin-embedded tissue block was sectioned at 5-um thickness on a microtome (Accu-Cut, Sakura Finetek Co., Ltd, Japan) and stained with haematoxylin for nuclear staining and eosin for cytoplasmic staining (H & E); stained areas were viewed using an optical microscope with a magnifying power of ×200. .. DPPH radical scavenging activity assay The effect of MSW on the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical was estimated according to the method of Yoshikawa et al. [ ] with a minor modification.

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: For H & E and Elastica van Gieson (EVG) staining, lungs were fixed with 4% paraformaldehyde and embedded in paraffin. .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: FK506 Induces Ligand-Independent Activation of the Bone Morphogenetic Protein Pathway and Osteogenesis
Article Snippet: The samples were then embedded in paraffin and cut into slices of 5 microns using a microtome (Accu-Cut SRM 200 Rotary Microtomoe, Sakura Finetek USA, Torrance, CA, USA). .. Slides were stained with Hematoxylin and Eosin (H & E) and Goldner’s trichrome (Sigma-Aldrich).

Article Title: The Effect of an Enamel Matrix Derivative (Emdogain) Combined with Bone Ceramic on Bone Formation in Mandibular Defects: A Histomorphometric and Immunohistochemical Study in the Canine
Article Snippet: .. With a microtome (ACCU-Cut SRM200, Sakura Finetek Europe, Alphen aan den Rijn, The Netherlands), buccolingual cross-sections were obtained from the middle portion of the defects and were ground to a final thickness of 4 μ m. Two slides of each specimen were stained with hematoxylin-eosin, and the other two slides were prepared for immunohistochemical analysis for markers of osteopontin (OPN) (Ncl-o-pontin, mouse monoclonal, Leica Biosystems, Newcastle, United Kingdom) by the polymer HRP method according to the manufacturer. .. Histologic and Histomorphometric Analyses Slides were examined by a blinded examiner under a light microscope (Olympus CX21FS, Olympus Corporation, Tokyo, Japan) at a magnification of ×100.

Article Title: Mouse Model of Enteropathogenic Escherichia coli Infection
Article Snippet: .. Tissue sections 5 μm thick were cut by use of a microtome (Sakura Finetek) and stained with hematoxylin and eosin (H & E) or periodic acid-Schiff (PAS) solutions. .. Images were acquired by use of a DMLB microscope equipped with Fluotar objectives (Leica Microsystems Inc., Bannockburn, Ill.) and a Micropublisher 5.0 camera (Q Imaging, Burnaby, British Columbia, Canada).

Article Title: Comparative study of pandemic (H1N1) 2009, swine H1N1, and avian H3N2 influenza viral infections in quails
Article Snippet: Histopathology Tissue samples collected from quails 3, 5, and 7 DPI were fixed at room temperature in 10% neutral buffered formalin solution for a minimum of 48 h. After fixation, the tissues were immediately embedded in paraffin and cut by microtome (Sakura Finetek, Japan) into 5-µm sections. .. For the histological examination, the sections were stained with hematoxylin and eosin and assigned a score of 0 to 3 based on the degree of inflammation by a veterinary pathologist (R.T.): 0, no lesion; 1, mild inflammation characterized by focal infiltration of inflammatory cells; 2, moderate inflammation characterized by multifocal infiltration of inflammatory cells; 3, severe inflammation characterized by diffuse infiltration of inflammatory cells.

Affinity Purification:

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide. .. Briefly, after blocking endogenous peroxidase and preincubation in 10% normal horse serum, the sections were incubated in primary rabbit polyclonal affinity purified anti-FosB antibody (sc-48, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) diluted 1∶600 in 0.1 M PBS with 0.1% Triton X-100 for 16 hours at room temperature.

Plasmid Preparation:

Article Title: Gene Expression Changes in the Olfactory Bulb of Mice Induced by Exposure to Diesel Exhaust Are Dependent on Animal Rearing Environment
Article Snippet: The brains were then frozen and cut in the coronal plane (6 series of 40- µm thick sections) on a microtome (Sakura Finetek Co., Ltd., Japan) and collected in 0.1 M PBS with 0.1% sodium azide. .. After three 10-minute rinses in 0.1 M PBS with 0.1% Triton X-100, the sections were further incubated in a biotinylated secondary antibody solution, donkey anti-rabbit IgG (AP182B, Chemicon, Temecula, CA, USA, 1∶800) for 120 minutes at room temperature, followed by three 10-minute rinses in 0.1 M PBS with 0.1% Triton X-100, and finally treated with an avidin-biotin peroxidase complex (Vectastain ABC peroxidase kit, Vector Laboratories Inc., Burlingame, CA, USA, 1∶400) for 240 minutes.

Software:

Article Title: Prolonged activation of IL-5–producing ILC2 causes pulmonary arterial hypertrophy
Article Snippet: Individual fluorescent images were acquired using a TCS SP5 confocal microscope and imaging system (Leica Microsystems), and trimmed and overlaid with Photoshop software (Adobe Systems). .. Samples were cut into 4-μm slices using a microtome (Sakura Finetek).

Article Title: FK506 Induces Ligand-Independent Activation of the Bone Morphogenetic Protein Pathway and Osteogenesis
Article Snippet: The samples were then embedded in paraffin and cut into slices of 5 microns using a microtome (Accu-Cut SRM 200 Rotary Microtomoe, Sakura Finetek USA, Torrance, CA, USA). .. Images were obtained with Lionheart LX (Biotek Instruments Inc., Winooski, VT, USA) at 4× and captured using Gen 5 software (Biotek Instruments Inc., Winooski, VT, USA)).

Article Title: Mouse Model of Enteropathogenic Escherichia coli Infection
Article Snippet: Tissue sections 5 μm thick were cut by use of a microtome (Sakura Finetek) and stained with hematoxylin and eosin (H & E) or periodic acid-Schiff (PAS) solutions. .. Images were collected by use of QCapture software 2.6.

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