Structured Review

Michrom microtome
Microtome, supplied by Michrom, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Michrom
Average 87 stars, based on 1 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
87/100 stars

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Related Articles

Immunohistochemistry:

Article Title: The Importance of Immunohistochemical Analyses in Evaluating the Phenotype of Kv Channel Knockout Mice
Article Snippet: Paragraph title: Immunohistochemistry ... The brains were removed, cryoprotected for 18–48 hr in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40 µm sagittal sections on a sliding microtome (Michrom HM 450, Richard-Allan Scientific, Kalamazoo, MI) equipped with a freezing stage (Physitemp BFS-30TC and PTU-3, Clifton, NJ).

Mouse Assay:

Article Title: Antibody-guided photoablation of voltage-gated potassium currents
Article Snippet: Immunofluorescence staining of brain sections Mice were deeply anesthetized with sodium pentobarbital (60 mg/kg, intraperitoneally) and perfused through the ascending aorta with 20 ml of 0.9% saline, followed by 80 ml of fixative containing 4% formaldehyde, prepared from freshly depolymerized paraformaldehyde, in 0.1 M PB. .. The brains were removed, cryoprotected for 18–48 h in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40-µm sagittal sections on a sliding microtome (Michrom HM 450; Richard-Allan Scientific) equipped with a freezing stage (BFS-30TC and PTU-3; Physitemp).

Article Title: The Importance of Immunohistochemical Analyses in Evaluating the Phenotype of Kv Channel Knockout Mice
Article Snippet: Mice were deeply anesthetized with sodium pentobarbital (60 mg/kg, i.p.) and perfused through the ascending aorta with 20 ml of 0.9% saline followed by 80 ml of fixative containing 4% formaldehyde, prepared from freshly depolymerized paraformaldehyde, in 0.1 M sodium phosphate buffer (PB), pH 7.4. .. The brains were removed, cryoprotected for 18–48 hr in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40 µm sagittal sections on a sliding microtome (Michrom HM 450, Richard-Allan Scientific, Kalamazoo, MI) equipped with a freezing stage (Physitemp BFS-30TC and PTU-3, Clifton, NJ).

Incubation:

Article Title: Antibody-guided photoablation of voltage-gated potassium currents
Article Snippet: The brains were removed, cryoprotected for 18–48 h in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40-µm sagittal sections on a sliding microtome (Michrom HM 450; Richard-Allan Scientific) equipped with a freezing stage (BFS-30TC and PTU-3; Physitemp). .. Sections were incubated overnight at 4°C in vehicle containing anti-Kv4.2 mouse mAb K57/1 and anti-Kv2.1 affinity-purified rabbit polyclonal antibody KC ( ).

Article Title: The Importance of Immunohistochemical Analyses in Evaluating the Phenotype of Kv Channel Knockout Mice
Article Snippet: The brains were removed, cryoprotected for 18–48 hr in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40 µm sagittal sections on a sliding microtome (Michrom HM 450, Richard-Allan Scientific, Kalamazoo, MI) equipped with a freezing stage (Physitemp BFS-30TC and PTU-3, Clifton, NJ). .. For immunohistochemistry, sections were incubated for 1 hr in 10 mM PB (pH 7.4) containing 0.9% NaCl (PBS) and 0.5% Triton X-100.

Staining:

Article Title: Antibody-guided photoablation of voltage-gated potassium currents
Article Snippet: Paragraph title: Immunofluorescence staining of brain sections ... The brains were removed, cryoprotected for 18–48 h in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40-µm sagittal sections on a sliding microtome (Michrom HM 450; Richard-Allan Scientific) equipped with a freezing stage (BFS-30TC and PTU-3; Physitemp).

Affinity Purification:

Article Title: Antibody-guided photoablation of voltage-gated potassium currents
Article Snippet: The brains were removed, cryoprotected for 18–48 h in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40-µm sagittal sections on a sliding microtome (Michrom HM 450; Richard-Allan Scientific) equipped with a freezing stage (BFS-30TC and PTU-3; Physitemp). .. Sections were incubated overnight at 4°C in vehicle containing anti-Kv4.2 mouse mAb K57/1 and anti-Kv2.1 affinity-purified rabbit polyclonal antibody KC ( ).

Immunofluorescence:

Article Title: Antibody-guided photoablation of voltage-gated potassium currents
Article Snippet: Paragraph title: Immunofluorescence staining of brain sections ... The brains were removed, cryoprotected for 18–48 h in 20% sucrose, frozen in a bed of pulverized dry ice, and then cut into 40-µm sagittal sections on a sliding microtome (Michrom HM 450; Richard-Allan Scientific) equipped with a freezing stage (BFS-30TC and PTU-3; Physitemp).

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  • 87
    Michrom microtome
    Microtome, supplied by Michrom, used in various techniques. Bioz Stars score: 87/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Michrom
    Average 87 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-04
    87/100 stars
      Buy from Supplier

    86
    Michrom freezing microtome
    Freezing Microtome, supplied by Michrom, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/freezing microtome/product/Michrom
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    freezing microtome - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

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