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Fisher Scientific microtome
Microtome, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Fisher Scientific
Average 93 stars, based on 16 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Hybridization:

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: After washes in hybridization buffer, samples were blocked in the presence of 2% blocking reagent (Roche Diagnostics) and 20% inactivated sheep serum. .. After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA).

Electron Microscopy:

Article Title: The Human Ubiquitin Conjugating Enzyme, UBE2E3, Is Required for Proliferation of Retinal Pigment Epithelial Cells
Article Snippet: Sections (5–10 μ m in thickness) were cut on a microtome (Cryocut 1800; Leica, Bannockburn, IL), placed on slides (Superfrost/Plus; Fisher Scientific, Pittsburgh, PA), air-dried, and washed with rinse buffer (0.1 M sodium phosphate buffer [pH 7.3], 2 mM MgCl2 , 0.1% Triton X-100). .. The samples were then counterstained with neutral red for 1 minute and overlaid with mounting medium (Cytoseal; Electron Microscopy Sciences, Fort Washington, PA) and a coverslip.

In Vivo:

Article Title: Relative contribution of clear cells and principal cells to luminal pH in the mouse epididymis
Article Snippet: Paragraph title: In vivo microperfusion of the mouse Cd and determination of luminal pH ... Tissues were cut in a microtome (Leica CM3050-S) at 5 or 10 μm thickness and picked up onto Fisher Superfrost/ Plus microscope slides (Fisher Scientific).

Amplification:

Article Title: The Human Ubiquitin Conjugating Enzyme, UBE2E3, Is Required for Proliferation of Retinal Pigment Epithelial Cells
Article Snippet: The disrupted UbcM2 allele was amplified with the UbcM2 exon 3–specific primer and a nested β -gal-neo–specific primer (5′-ATTCAGGCTGCGCAACTGTTGGG-3′). .. Sections (5–10 μ m in thickness) were cut on a microtome (Cryocut 1800; Leica, Bannockburn, IL), placed on slides (Superfrost/Plus; Fisher Scientific, Pittsburgh, PA), air-dried, and washed with rinse buffer (0.1 M sodium phosphate buffer [pH 7.3], 2 mM MgCl2 , 0.1% Triton X-100).

In Situ:

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: In situ hybridizations were performed on whole-mounts (retina and vestibular end organs) and on cryostat sections of the cerebellum as previously described . .. After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA).

Software:

Article Title: Adult sox10+ Cardiomyocytes Contribute to Myocardial Regeneration in the Zebrafish
Article Snippet: All histological staining were performed on 7 μm paraffin sections cut on a microtome (Leica and Reichert-Jung), mounted on Superfrost slides (Fisher Scientific), and dried overnight at 37°C. .. ImageJ software was used to quantify cryoinjured area in uninjured and injured hearts.

Isolation:

Article Title: Requirement of transcription factor NFAT in developing atrial myocardium
Article Snippet: After washing three times in xylene (10 min each), isolated hearts were immersed in paraffin (three times, 15 min each), oriented, and embedded. .. Embedded hearts were serially sectioned sagittally at a thickness of 7 μm using a microtome (Microm; Baxter Scientific), and were placed on super-frost plus slides (Fisher Scientific).

Article Title: Cranial Osteogenesis and Suture Morphology in Xenopus laevis: A Unique Model System for Studying Craniofacial Development
Article Snippet: Histological sections To track morphogenesis of sutures, we performed histological analyses from representative skulls isolated from the three groups, pre-metamorphosis, metamorphosis, and post-metamorphosis stages. .. Five micron histological sections were obtained in the coronal plane using a microtome (Micro HM-320) and mounted on Superfrost plus slides (Fisher Scientific).

Dissection:

Article Title: Three Dimensional Mesoscale Analysis of Translamellar Cross-Bridge Morphologies in the Annulus Fibrosus using Optical Coherence Tomography
Article Snippet: Paraffin blocks were cut with a microtome (HM355; Microm/Thermo Fisher Scientific, Waltham, MA, USA) to obtain 10 μm thick transverse sections of the AF. .. For additional verification that formalin had little effect on cross-bridge structure during imaging, a freshly cut porcine AF sample was imaged using both a regular dissection microscope and OCT. After 10% formalin fixation and histological staining with Safranin-O/Fast green described above, the same AF sample was examined under brightfield microscopy.

Transferring:

Article Title: Electrophysiological Evidence that Ethanol Alters Function of Medial Septal Area Without Affecting Lateral Septal Function 1
Article Snippet: For histological verification of the recording site, an anodal d.c. current of 20 μ A was passed for 45 sec through the recording pipette that contained a 2 M NaCl solution saturated with sky blue dye. .. With the use of a cryostat and microtome (Damon/IEC Division, Needham Hts., MA), 50- μ m slices were sectioned, mounted onto slides and stained with cresyl violet (Fisher Scientific, Pittsburgh, PA).

Microscopy:

Article Title: Relative contribution of clear cells and principal cells to luminal pH in the mouse epididymis
Article Snippet: .. Tissues were cut in a microtome (Leica CM3050-S) at 5 or 10 μm thickness and picked up onto Fisher Superfrost/ Plus microscope slides (Fisher Scientific). .. Double-immunofluorescence labeling for the B1 subunit of the V-ATPase (ATP6V1B1), specific for CCs, and clathrin, a subapical marker of CCs and PCs, was performed on cryosections, as previously described [ , , ].

Article Title: Alterations in Marginal Zone Macrophages and Marginal Zone B Cells in Old Mice
Article Snippet: .. Paraffin sections were sectioned on a microtome (4 μm) onto Superfrost Plus microscope slides (Fisher Scientific, Pittsburg, PA). .. The splenic sections were stained with H & E. Tissues were viewed and photographed with a Leitz Diaplan microscope (W. Nuhsbaum, McHenry, IL).

Article Title: Requirement of transcription factor NFAT in developing atrial myocardium
Article Snippet: Embedded hearts were serially sectioned sagittally at a thickness of 7 μm using a microtome (Microm; Baxter Scientific), and were placed on super-frost plus slides (Fisher Scientific). .. Embedded hearts were serially sectioned sagittally at a thickness of 7 μm using a microtome (Microm; Baxter Scientific), and were placed on super-frost plus slides (Fisher Scientific).

Article Title: Cranial Osteogenesis and Suture Morphology in Xenopus laevis: A Unique Model System for Studying Craniofacial Development
Article Snippet: Five micron histological sections were obtained in the coronal plane using a microtome (Micro HM-320) and mounted on Superfrost plus slides (Fisher Scientific). .. The sections were analyzed and photographed with a Zeiss Axioplan 2 microscope equipped with an Axiocam HRc digital camera.

Article Title: Three Dimensional Mesoscale Analysis of Translamellar Cross-Bridge Morphologies in the Annulus Fibrosus using Optical Coherence Tomography
Article Snippet: Paraffin blocks were cut with a microtome (HM355; Microm/Thermo Fisher Scientific, Waltham, MA, USA) to obtain 10 μm thick transverse sections of the AF. .. For additional verification that formalin had little effect on cross-bridge structure during imaging, a freshly cut porcine AF sample was imaged using both a regular dissection microscope and OCT. After 10% formalin fixation and histological staining with Safranin-O/Fast green described above, the same AF sample was examined under brightfield microscopy.

Size-exclusion Chromatography:

Article Title: Electrophysiological Evidence that Ethanol Alters Function of Medial Septal Area Without Affecting Lateral Septal Function 1
Article Snippet: A cathodal 20 μ A current was applied to the stimulation electrode for 45 sec. .. With the use of a cryostat and microtome (Damon/IEC Division, Needham Hts., MA), 50- μ m slices were sectioned, mounted onto slides and stained with cresyl violet (Fisher Scientific, Pittsburgh, PA).

Incubation:

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: They were then incubated with anti-DIG-alkaline-phosphatase (AP)-conjugated antibody (Roche Diagnostics), washed and revealed with NBT/BCIP staining. .. After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA).

Blocking Assay:

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: After washes in hybridization buffer, samples were blocked in the presence of 2% blocking reagent (Roche Diagnostics) and 20% inactivated sheep serum. .. After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA).

Article Title: Relative contribution of clear cells and principal cells to luminal pH in the mouse epididymis
Article Snippet: Tissues were embedded in Tissue-Tek OCT compound (Sakura Fintek, Torrance, CA, USA), and mounted and frozen on a cutting block. .. Tissues were cut in a microtome (Leica CM3050-S) at 5 or 10 μm thickness and picked up onto Fisher Superfrost/ Plus microscope slides (Fisher Scientific).

Inverted Microscopy:

Article Title: Toward a patient-specific tissue engineered vascular graft
Article Snippet: Sections (10 µm thick) were prepared via microtome (Leica CM 1950) and mounted on positively charged slides (Superfrost™ Plus, Fisher Scientific). .. Dark field photomicrographs were obtained with a Zeiss Axio Observer Z1 inverted microscope using an 89 North PhotoFluor LM-75 light source and the appropriate fluorescent filter at 20× magnification.

Staining:

Article Title: Toward a patient-specific tissue engineered vascular graft
Article Snippet: Sections (10 µm thick) were prepared via microtome (Leica CM 1950) and mounted on positively charged slides (Superfrost™ Plus, Fisher Scientific). .. Slides were air-dried at room temperature for 30 min and then sections fixed with 10% neutral buffered formalin (Fisher Scientific) for 10 min. After two consecutive washes with 1× PBS 0.05% Tween-20 (5 min per wash), slides were stained with 4′,6-diamidino-2-phenylindole (DAPI, SlowFade™ Gold Antifade Reagent with DAPI, ThermoFisher Scientific) and coverslipped.

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: .. After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA). .. Western Blotting The retina, cerebellum, vestibular end organs (maculae and cristae), and vestibular ganglia were dissected in cold 0.1M PBS (pH 7.4) and homogenized in RIPA buffer (Sigma, St. Louis, MO) containing 50 mM Tris-HCl, 150 mM sodium chloride, 1% nonyl phenoxylpolyethoxylethanol-40 (NP-40), 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), and protease inhibitor cocktail (Sigma, St. Louis, MO).

Article Title: The Human Ubiquitin Conjugating Enzyme, UBE2E3, Is Required for Proliferation of Retinal Pigment Epithelial Cells
Article Snippet: Paragraph title: Mouse Model and β -Galactosidase Staining ... Sections (5–10 μ m in thickness) were cut on a microtome (Cryocut 1800; Leica, Bannockburn, IL), placed on slides (Superfrost/Plus; Fisher Scientific, Pittsburgh, PA), air-dried, and washed with rinse buffer (0.1 M sodium phosphate buffer [pH 7.3], 2 mM MgCl2 , 0.1% Triton X-100).

Article Title: Electrophysiological Evidence that Ethanol Alters Function of Medial Septal Area Without Affecting Lateral Septal Function 1
Article Snippet: .. With the use of a cryostat and microtome (Damon/IEC Division, Needham Hts., MA), 50- μ m slices were sectioned, mounted onto slides and stained with cresyl violet (Fisher Scientific, Pittsburgh, PA). ..

Article Title: Alterations in Marginal Zone Macrophages and Marginal Zone B Cells in Old Mice
Article Snippet: For H & E staining, spleens were fixed in 10% buffered formalin and processed using an automatic tissue processor, dehydrated through graded alcohols, cleared in xylene, and infiltrated with paraffin. .. Paraffin sections were sectioned on a microtome (4 μm) onto Superfrost Plus microscope slides (Fisher Scientific, Pittsburg, PA).

Article Title: Adult sox10+ Cardiomyocytes Contribute to Myocardial Regeneration in the Zebrafish
Article Snippet: .. All histological staining were performed on 7 μm paraffin sections cut on a microtome (Leica and Reichert-Jung), mounted on Superfrost slides (Fisher Scientific), and dried overnight at 37°C. ..

Article Title: Cranial Osteogenesis and Suture Morphology in Xenopus laevis: A Unique Model System for Studying Craniofacial Development
Article Snippet: Five micron histological sections were obtained in the coronal plane using a microtome (Micro HM-320) and mounted on Superfrost plus slides (Fisher Scientific). .. Every 10th to 15th slide was stained with Movat's pentachrome for bone and cartilage identification.

Article Title: Three Dimensional Mesoscale Analysis of Translamellar Cross-Bridge Morphologies in the Annulus Fibrosus using Optical Coherence Tomography
Article Snippet: Paraffin blocks were cut with a microtome (HM355; Microm/Thermo Fisher Scientific, Waltham, MA, USA) to obtain 10 μm thick transverse sections of the AF. .. Sections were stained with Safranin-O/Fast green and examined under brightfield.

Mouse Assay:

Article Title: Deletion of KCNQ2/3 potassium channels from PV+ interneurons leads to homeostatic potentiation of excitatory transmission
Article Snippet: Mice (P8-P35) were anesthetized with isoflurane and rapidly decapitated. .. Coronal or Transverse hippocampus slices including surrounding structures were cut at 300 μm using a vibrating microtome (Microm HM 650V-Thermo Fisher Scientific; or Leica VT1200S).

Article Title: The Human Ubiquitin Conjugating Enzyme, UBE2E3, Is Required for Proliferation of Retinal Pigment Epithelial Cells
Article Snippet: For the data shown in Figure 6, the mice were euthanatized, and cryosections were prepared from embryonic day (E)13 heads and postnatal day (P)17 eyes. .. Sections (5–10 μ m in thickness) were cut on a microtome (Cryocut 1800; Leica, Bannockburn, IL), placed on slides (Superfrost/Plus; Fisher Scientific, Pittsburgh, PA), air-dried, and washed with rinse buffer (0.1 M sodium phosphate buffer [pH 7.3], 2 mM MgCl2 , 0.1% Triton X-100).

In Situ Hybridization:

Article Title: Glutamate Transporters EAAT4 and EAAT5 Are Expressed in Vestibular Hair Cells and Calyx Endings
Article Snippet: Paragraph title: In situ Hybridization ... After staining, samples were washed in PBS, embedded in 4% agarose (Invitrogen, Carlsbad, CA) in PBS and cut into 30 µm sections in ice-cold PBS, using a vibrating microtome (Microm/Thermo Fisher Scientific, Waltham MA).

Imaging:

Article Title: Toward a patient-specific tissue engineered vascular graft
Article Snippet: Paragraph title: Dark field imaging of seeded scaffold ... Sections (10 µm thick) were prepared via microtome (Leica CM 1950) and mounted on positively charged slides (Superfrost™ Plus, Fisher Scientific).

Article Title: Three Dimensional Mesoscale Analysis of Translamellar Cross-Bridge Morphologies in the Annulus Fibrosus using Optical Coherence Tomography
Article Snippet: Paragraph title: 2.3. Histological validation of translamellar cross-bridge imaging by OCT ... Paraffin blocks were cut with a microtome (HM355; Microm/Thermo Fisher Scientific, Waltham, MA, USA) to obtain 10 μm thick transverse sections of the AF.

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