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Campden Instruments microtome
Microtome, supplied by Campden Instruments, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microtome/product/Campden Instruments
Average 99 stars, based on 48 article reviews
Price from $9.99 to $1999.99
microtome - by Bioz Stars, 2020-03
99/100 stars

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Related Articles

Flow Cytometry:

Article Title: Temperature dependence of vesicular dynamics at excitatory synapses of rat hippocampus
Article Snippet: Hippocampal slices (400–450 μm thick) were cut using a vibrating microtome (Campden, United Kingdom) and incubated at room temperature in ACSF for 1 h or more before use. .. The slices were subsequently transferred to a recording chamber in which they were kept fully submerged in ACSF at 20–22 °C, and with flow of 3–4 ml/min.

Article Title: Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus
Article Snippet: Coronal hippocampal slices (350 μm) were prepared using a vibrating microtome (7000smz-2, Campden Instruments LTD, Loughborough, UK) in ice-cold sucrose-based cutting solution oxygenated with carbogen gas (95% O2 , 5% CO2 ) and with the following composition (in mM): KCl 3, NaH2 PO4 1.25, MgSO4 2, MgCl2 1, CaCl2 1, NaHCO3 26.4, glucose 10, sucrose 206, ascorbic acid 0.40, kynurenic acid 1. .. Slices were then individually transferred to the recording chamber and were perfused with oxygenated aCSF at room temperature at a flow-rate of approximately 2 mL/min.

Immunocytochemistry:

Article Title: Sex differences in brain developing in the presence or absence of gonads
Article Snippet: Paragraph title: Immunocytochemistry on floating sections ... Brains were embedded in 5% agarose (Sigma) and sectioned at 50 μm in cold 0.01M PBS using a vibrating microtome (Integraslice 7550 MM, Campden Instruments, UK).

In Vitro:

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: Paragraph title: In vitro spinal cord slice preparation. ... Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments).

Fluorescence:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.). .. Fluoro-Gold stainings in the PVN, SCN, DMH, VMH, and ARC were imaged under a fluorescence microscope using an excitation wavelength range of 340 to 380 nm, and emission was measured using a 610-nm long-pass filter.

Article Title: Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit
Article Snippet: The brain was glued to a vibrating microtome (Campden Instruments, Loughborough, UK) and 200-μm thick coronal sections of the brainstem containing the NTS were prepared. .. For whole-cell recordings, slices were transferred to a submerged chamber and a Slicescope upright microscope (Scientifica, Uckfield, UK) was used for infrared - differential interference contrast and fluorescence visualization of cells.

Patch Clamp:

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: Paragraph title: Hippocampal slice preparation for patch-clamp recordings ... The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England).

Isolation:

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: The lumbar spinal cord was isolated and immersed in low-melting-point agarose (3% in above solution) at 37°C, which was then cooled on ice. .. Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments).

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: .. The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England). ..

Dissection:

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: At P56–P70, mice were deeply anesthetized with sodium pentobarbital (60 mg/kg) and perfused with ice-cold dissection solution consisting of the following (in m m ): 250 sucrose, 2.5 KCl, 25 NaHCO3 , 1.0 NaH2 PO4 , 6 MgCl2 , 0.5 CaCl2 , and 25 glucose continuously bubbled with 95% O2 /5% CO2 . .. Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments).

Labeling:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: Paragraph title: Fluoro-Gold labeling ... Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.).

Mouse Assay:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: Fluoro-Gold labeling To label neurons that send monosynaptic projections to the ME, GAD67+/GFP mice received intraperitoneal injections of the retrogradely transportable marker compound Fluoro-Gold (40 mg/kg; Wako) ( ). .. Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.).

Article Title: Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit
Article Snippet: Electrophysiological studies For electrophysiological validation mice were anesthetized with sodium pentobarbital (Euthatal) and decapitated. .. The brain was glued to a vibrating microtome (Campden Instruments, Loughborough, UK) and 200-μm thick coronal sections of the brainstem containing the NTS were prepared.

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: At P56–P70, mice were deeply anesthetized with sodium pentobarbital (60 mg/kg) and perfused with ice-cold dissection solution consisting of the following (in m m ): 250 sucrose, 2.5 KCl, 25 NaHCO3 , 1.0 NaH2 PO4 , 6 MgCl2 , 0.5 CaCl2 , and 25 glucose continuously bubbled with 95% O2 /5% CO2 . .. Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments).

Article Title: The LIM Homeodomain Protein Lhx6 Regulates Maturation of Interneurons and Network Excitability in the Mammalian Cortex
Article Snippet: Briefly, mice were decapitated and the brain was rapidly removed and placed in ice-cold sucrose solution containing: 75 mM sucrose, 87 mM NaCl, 2.5 mM KCl, 0.5 mM CaCl2 , 7 mM MgCl2 , 1.0 mM NaH2 PO4 , 25 mM NaHCO3 , and 25 mM glucose. .. Transverse hippocampal slices (300 µm thickness) were cut using a vibrating microtome (Campden Instruments).

Article Title: Long-Term Isolation Elicits Depression and Anxiety-Related Behaviors by Reducing Oxytocin-Induced GABAergic Transmission in Central Amygdala
Article Snippet: .. Briefly, coronal slices containing the CeA (300 μm) were obtained from 11- to 12-week-old mice using a vibrating microtome (Model 7000smz-2, Campden Instrument, United Kingdom) after isoflurane anesthesia and decapitation. ..

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: Mice were anesthetized by isoflurane inhalation and decapitated into ice-cold slicing solution (in mM): 87 NaCl, 2.5 KCl, 1.25 NaH2 PO4 , 25 NaHCO3 , 7 MgCl2 , 0.5 CaCl2 , 25 D-glucose, 75 sucrose and (osmolality ~340 mmol/kg). .. The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England).

Article Title: Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus
Article Snippet: Electrophysiology For electrophysiological recordings, SiRCRE-mCherry was injected bilaterally in the CA1 of one month-old Rosa-LoxP-STOP-LoxP-tdTomato or Rosa-LoxP-STOP-LoxP-ChR2YFP mice. .. Coronal hippocampal slices (350 μm) were prepared using a vibrating microtome (7000smz-2, Campden Instruments LTD, Loughborough, UK) in ice-cold sucrose-based cutting solution oxygenated with carbogen gas (95% O2 , 5% CO2 ) and with the following composition (in mM): KCl 3, NaH2 PO4 1.25, MgSO4 2, MgCl2 1, CaCl2 1, NaHCO3 26.4, glucose 10, sucrose 206, ascorbic acid 0.40, kynurenic acid 1.

Slice Preparation:

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: Paragraph title: In vitro spinal cord slice preparation. ... Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments).

Article Title: Long-Term Isolation Elicits Depression and Anxiety-Related Behaviors by Reducing Oxytocin-Induced GABAergic Transmission in Central Amygdala
Article Snippet: Paragraph title: Slice Preparation ... Briefly, coronal slices containing the CeA (300 μm) were obtained from 11- to 12-week-old mice using a vibrating microtome (Model 7000smz-2, Campden Instrument, United Kingdom) after isoflurane anesthesia and decapitation.

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: Paragraph title: Hippocampal slice preparation for patch-clamp recordings ... The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England).

Incubation:

Article Title: Albumin elicits calcium signals from astrocytes in brain slices from neonatal rat cortex
Article Snippet: Cortical slices were cut on a vibrating microtome (Vibraslice, Campden Instruments, Loughborough, UK) and were allowed to recover for 30-60 min in a medium containing (mM): 140 NaCl, 5 KCl, 1.2 KH2 PO4 , 1.3 MgSO4 , 26 NaHCO3 , 2.4 CaCl2 and 10 glucose, continuously gassed with 5 % CO2 -95 % O2 . .. After the recovery period slices were loaded with calcium indicator by incubation in 10 μM of either fura-2 AM or fluo-3 AM (Molecular Probes), for at least 1 h at room temperature.

Article Title: Sex differences in brain developing in the presence or absence of gonads
Article Snippet: Brains were embedded in 5% agarose (Sigma) and sectioned at 50 μm in cold 0.01M PBS using a vibrating microtome (Integraslice 7550 MM, Campden Instruments, UK). .. Sections were incubated in 0.1M glycine (Sigma) in 0.01M PBS for 30 min followed by incubation in 0.5% sodium borohydride (Sigma) for 15 min at 4°C.

Article Title: Aberrant Synaptic Integration in Adult Lamina I Projection Neurons Following Neonatal Tissue Damage
Article Snippet: Parasagittal slices (350–400 μm) with the L4–L5 dorsal roots attached (length 7–10 mm) were cut from the side ipsilateral to the injury using a vibrating microtome (7000smz-2; Campden Instruments). .. Slices were incubated for 15–20 min in a recovery solution containing the following (in m m ): 92 NMDG, 2.5 KCl, 1.2 NaH2 PO4 , 30 NaHCO3 , 20 HEPES, 25 glucose, 5 Na ascorbate, 2 thiourea, 3 Na pyruvate, 10 MgSO4 , and 0.5 CaCl2 , and then allowed to recover further in an oxygenated artificial CSF (aCSF) solution containing the following (in m m ): 125 NaCl, 2.5 KCl, 25 NaHCO3 , 1.0 NaH2 PO4 , 1.0 MgCl2 , 2.0 CaCl2 , and 25 glucose for ≥1 h at room temperature.

Article Title: Temperature dependence of vesicular dynamics at excitatory synapses of rat hippocampus
Article Snippet: .. Hippocampal slices (400–450 μm thick) were cut using a vibrating microtome (Campden, United Kingdom) and incubated at room temperature in ACSF for 1 h or more before use. .. The slices were subsequently transferred to a recording chamber in which they were kept fully submerged in ACSF at 20–22 °C, and with flow of 3–4 ml/min.

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England). .. Slices were then incubated at 37°C for 35 min in artificial cerebrospinal fluid (ACSF) containing (in mM): 120 NaCl, 2.5 KCl, 1 NaH2 PO4 , 26 NaHCO3 , 1.3 MgCl2 , 2.5 CaCl2 , 10 D-glucose (~300 mmol/Kg, pH 7.4).

Article Title: Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus
Article Snippet: Coronal hippocampal slices (350 μm) were prepared using a vibrating microtome (7000smz-2, Campden Instruments LTD, Loughborough, UK) in ice-cold sucrose-based cutting solution oxygenated with carbogen gas (95% O2 , 5% CO2 ) and with the following composition (in mM): KCl 3, NaH2 PO4 1.25, MgSO4 2, MgCl2 1, CaCl2 1, NaHCO3 26.4, glucose 10, sucrose 206, ascorbic acid 0.40, kynurenic acid 1. .. Slices were incubated at 37°C for 30 min in a submerged-style holding chamber with oxygenated artificial cerebrospinal fluid (aCSF; in mM: NaCl 126, KCl 3, NaH2 PO4 1.25, MgSO4 2, CaCl2 2, NaHCO3 26.4, glucose 10) with an osmolarity adjusted to 280-300 mOsm/L and stored thereafter in the same holding chamber at room temperature for at least 1 hr.

other:

Article Title: Hibernation induces pentobarbital insensitivity in medulla but not cortex
Article Snippet: Medullary and cortical slices (350 μm thick) were cut with a vibrating microtome (Campden Instruments, Layfayette, IN).

Article Title: Increased GABAA Receptor ?-Subunit Expression on Ventral Respiratory Column Neurons Protects Breathing during Pregnancy
Article Snippet: Experimental protocol Brains were removed and coronal medullary and cortical slices were cut in ice-cold 3 mM KCl aCSF with a vibrating microtome (Campden Instruments, Layfayette, IN).

Article Title: A Scorpion α-Like Toxin That Is Active on Insects and Mammals Reveals an Unexpected Specificity and Distribution of Sodium Channel Subtypes in Rat Brain Neurons
Article Snippet: Neurons were visualized using a Zeiss axioscop, equipped with a 40 × 0.75 NA water immersion objective and DIC optics, and an infrared (IR)-sensitive video camera (range, 400–800 nm; type –07; Hamamatsu, Tokyo, Japan).

Microscopy:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.). .. Fluoro-Gold stainings in the PVN, SCN, DMH, VMH, and ARC were imaged under a fluorescence microscope using an excitation wavelength range of 340 to 380 nm, and emission was measured using a 610-nm long-pass filter.

Article Title: Albumin elicits calcium signals from astrocytes in brain slices from neonatal rat cortex
Article Snippet: Cortical slices were cut on a vibrating microtome (Vibraslice, Campden Instruments, Loughborough, UK) and were allowed to recover for 30-60 min in a medium containing (mM): 140 NaCl, 5 KCl, 1.2 KH2 PO4 , 1.3 MgSO4 , 26 NaHCO3 , 2.4 CaCl2 and 10 glucose, continuously gassed with 5 % CO2 -95 % O2 . .. Slices were transferred to the stage of an upright microscope for calcium imaging (see below) and were kept in position with a nylon net.

Article Title: Appetite controlled by a cholecystokinin nucleus of the solitary tract to hypothalamus neurocircuit
Article Snippet: The brain was glued to a vibrating microtome (Campden Instruments, Loughborough, UK) and 200-μm thick coronal sections of the brainstem containing the NTS were prepared. .. For whole-cell recordings, slices were transferred to a submerged chamber and a Slicescope upright microscope (Scientifica, Uckfield, UK) was used for infrared - differential interference contrast and fluorescence visualization of cells.

Article Title: The LIM Homeodomain Protein Lhx6 Regulates Maturation of Interneurons and Network Excitability in the Mammalian Cortex
Article Snippet: Transverse hippocampal slices (300 µm thickness) were cut using a vibrating microtome (Campden Instruments). .. Whole-cell voltage-clamp recordings were obtained from visually identified granule cells using an infrared differential interference contrast video microscopy system.

Transmission Assay:

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England). .. Additionally, all patch-clamp recording solutions contained 100 µM picrotoxin and 1 mM kynurenic acid to block fast synaptic transmission.

Marker:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: Fluoro-Gold labeling To label neurons that send monosynaptic projections to the ME, GAD67+/GFP mice received intraperitoneal injections of the retrogradely transportable marker compound Fluoro-Gold (40 mg/kg; Wako) ( ). .. Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.).

Injection:

Article Title: A novel GABA-mediated corticotropin-releasing hormone secretory mechanism in the median eminence
Article Snippet: .. Four days after the injection, the brains were removed, and 100-μm-thick coronal brain sections were obtained using a vibrating microtome (Campden 7000smz-2; Campden Instruments Ltd.). .. Fluoro-Gold stainings in the PVN, SCN, DMH, VMH, and ARC were imaged under a fluorescence microscope using an excitation wavelength range of 340 to 380 nm, and emission was measured using a 610-nm long-pass filter.

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: For BAD reconstitution experiments, mice were injected (at postnatal day 1 or 2) in both hemispheres with 200 nl of AAV2/8.CAG.BAD.2A.mCherry. .. The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England).

Article Title: Life-Long Genetic and Functional Access to Neural Circuits Using Self-Inactivating Rabies Virus
Article Snippet: Electrophysiology For electrophysiological recordings, SiRCRE-mCherry was injected bilaterally in the CA1 of one month-old Rosa-LoxP-STOP-LoxP-tdTomato or Rosa-LoxP-STOP-LoxP-ChR2YFP mice. .. Coronal hippocampal slices (350 μm) were prepared using a vibrating microtome (7000smz-2, Campden Instruments LTD, Loughborough, UK) in ice-cold sucrose-based cutting solution oxygenated with carbogen gas (95% O2 , 5% CO2 ) and with the following composition (in mM): KCl 3, NaH2 PO4 1.25, MgSO4 2, MgCl2 1, CaCl2 1, NaHCO3 26.4, glucose 10, sucrose 206, ascorbic acid 0.40, kynurenic acid 1.

Blocking Assay:

Article Title: Temperature dependence of vesicular dynamics at excitatory synapses of rat hippocampus
Article Snippet: Hippocampal slices (400–450 μm thick) were cut using a vibrating microtome (Campden, United Kingdom) and incubated at room temperature in ACSF for 1 h or more before use. .. Cesium was chosen as the main cation in order to block potassium conductances.

Article Title: BAD and KATP channels regulate neuron excitability and epileptiform activity
Article Snippet: The hippocampus was isolated and mounted on a 5% agar cube which was then glued on the stage of a vibrating microtome (Campden Instuments 7000smz, Loughborough, England). .. Additionally, all patch-clamp recording solutions contained 100 µM picrotoxin and 1 mM kynurenic acid to block fast synaptic transmission.

Imaging:

Article Title: Albumin elicits calcium signals from astrocytes in brain slices from neonatal rat cortex
Article Snippet: Cortical slices were cut on a vibrating microtome (Vibraslice, Campden Instruments, Loughborough, UK) and were allowed to recover for 30-60 min in a medium containing (mM): 140 NaCl, 5 KCl, 1.2 KH2 PO4 , 1.3 MgSO4 , 26 NaHCO3 , 2.4 CaCl2 and 10 glucose, continuously gassed with 5 % CO2 -95 % O2 . .. Slices were transferred to the stage of an upright microscope for calcium imaging (see below) and were kept in position with a nylon net.

Article Title: A synaptic threshold mechanism for computing escape decisions
Article Snippet: Acute slices (150μm) were cut using a microtome (Campden 7000smz-2 or Leica VT1200S) in ice-cold PBS (0.1M), directly transferred to 4% PFA solution, and kept for 20min at 4°C. .. The slices were then rinsed in PBS, counter-stained with DAPI (3μM in PBS), and mounted on slides in SlowFade Gold (Life Technologies) before wide-field imaging (Nikon TE2000) on the same day to confirm the site of infusion.

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    Campden Instruments microtome
    Microtome, supplied by Campden Instruments, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microtome/product/Campden Instruments
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    microtome - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

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