microseal  (Bio-Rad)

 
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  • 95
    Name:
    Microseal F PCR Plate Seal
    Description:
    Pkg of 100 aluminized foil seal for PCR plates pierceable
    Catalog Number:
    MSF1001
    Price:
    None
    Category:
    96 Well PCR Plates
    Buy from Supplier


    Structured Review

    Bio-Rad microseal
    Microseal F PCR Plate Seal
    Pkg of 100 aluminized foil seal for PCR plates pierceable
    https://www.bioz.com/result/microseal/product/Bio-Rad
    Average 95 stars, based on 144 article reviews
    Price from $9.99 to $1999.99
    microseal - by Bioz Stars, 2020-07
    95/100 stars

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    Related Articles

    Irradiation:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Flow Cytometry:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Next-Generation Sequencing:

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674) .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674)

    Transferring:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Cytometry:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Cell Culture:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Electrophoresis:

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674) .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674)

    Spectrophotometry:

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: .. Round-bottom 96-well plates (Corning, cat. no. 3788) 50 ml sterile reagent troughs (Corning, cat. no. 4871) Thin-walled 96-well PCR microtiter plates (Bio-Rad, cat. no. HSP-9601) 15 cm sterile petri dishes (Fisher Scientific, cat. no. 08-757-14) Aluminum foil microplate seal (Bio-Rad, cat. no. MSF-1001) 50 ml polypropylene tubes (BD Falcon, cat. no. 352070) Tissue culture 96 wells flat bottom plate (BD Falcon, cat. no. 353075) PIPETMAN P10, 1 to 10 µl (Gibson, cat. no. F144802) PIPETMAN P200, 50 to 200 µl (Gibson, cat. no. F123601) Corning™ 4084 P10 multichannel pipettes 1 - 10 µl (Thermofisher scientific, cat. no. 07-764-710) Corning™ 4085 P50 multichannel pipettes 5 - 50 µl (Thermofisher scientific, cat. no. 07-764-711) Corning™ 4085 P200 multichannel pipettes 20 - 200 µl (Thermofisher scientific, cat. no. 07-764-712) Corning® Stripettor™ Ultra Pipet Controller (Thermofisher scientific, cat. no. 4099) Thermocycler capable of handling 96-well plates (Bio-Rad, cat. no. T100™) Temperature controlled incubator (30°C) (Thermofisher scientific, cat. no. 15-103-0515) Temperature controlled water bath (42°C) (Thermofisher scientific, cat. no. FSGPD10) 1% E-Gel® 96 Agarose Gels (Thermofisher scientific, cat. no. G700801) E-gel Mother E-Base capable of running 96 samples (Thermofisher scientific, cat. no. EBM03) Temperature controlled shaker for 250 ml flasks and 50 ml tubes (MaxQ™ 6000 Shaker, Thermofisher scientific, cat. no. SHKE6000) Benchtop Centrifuge suitable for microplates and 50 ml tubes (Sorvall™ Legend™ XT/XF centrifuge, Thermofisher scientific, cat. no. 75-004-505) Plate shaker (Mixmate, Eppendorf, cat. no. 022674200) GENESYS™ 10S UV-Vis Spectrophotometer (Thermofisher scientific, cat. no. 840-208100) Vortexer (Thermofisher scientific, cat. no. 88880017TS) Centro XS3 Luminescence Microplate Reader (Berthold Technologies, cat. no. 46970) 1300 Series A2 Class II, Type A2 Bio Safety Cabinets (Thermofisher scientific, cat. no. 13-261-221) Optional: liquid handling robot with 96-well multichannel head and individual well cherry-picking capability (Tecan, cat. no. Fluent 780) Optional: BioRobot Universal System Robot with 96-well DNA miniprep capability (Qiagen, cat. no. 9001094) .. Custom DNA primers, 50 µM in a 96-well format, no PAGE purification required (See ; Sigma-Aldrich)

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674) .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674)

    Inverted Microscopy:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

    Polymerase Chain Reaction:

    Article Title: Development of TaqMan probes targeting the four major celiac disease epitopes found in α-gliadin sequences of spelt (Triticum aestivum ssp. spelta) and bread wheat (Triticum aestivum ssp. aestivum)
    Article Snippet: .. Samples were loaded in a Hard-Shell® 96-Well PCR skirted white plate and sealed with a Microseal® ‘B’ PCR Plate Sealing Film (Bio-Rad). .. PCR amplifications were performed by the C1000 Touch™ Thermal Cycler coupled to the CFX96™ Real-Time detection system (Bio-Rad).

    Article Title: Base-resolution stratification of cancer mutations using functional variomics
    Article Snippet: .. Round-bottom 96-well plates (Corning, cat. no. 3788) 50 ml sterile reagent troughs (Corning, cat. no. 4871) Thin-walled 96-well PCR microtiter plates (Bio-Rad, cat. no. HSP-9601) 15 cm sterile petri dishes (Fisher Scientific, cat. no. 08-757-14) Aluminum foil microplate seal (Bio-Rad, cat. no. MSF-1001) 50 ml polypropylene tubes (BD Falcon, cat. no. 352070) Tissue culture 96 wells flat bottom plate (BD Falcon, cat. no. 353075) PIPETMAN P10, 1 to 10 µl (Gibson, cat. no. F144802) PIPETMAN P200, 50 to 200 µl (Gibson, cat. no. F123601) Corning™ 4084 P10 multichannel pipettes 1 - 10 µl (Thermofisher scientific, cat. no. 07-764-710) Corning™ 4085 P50 multichannel pipettes 5 - 50 µl (Thermofisher scientific, cat. no. 07-764-711) Corning™ 4085 P200 multichannel pipettes 20 - 200 µl (Thermofisher scientific, cat. no. 07-764-712) Corning® Stripettor™ Ultra Pipet Controller (Thermofisher scientific, cat. no. 4099) Thermocycler capable of handling 96-well plates (Bio-Rad, cat. no. T100™) Temperature controlled incubator (30°C) (Thermofisher scientific, cat. no. 15-103-0515) Temperature controlled water bath (42°C) (Thermofisher scientific, cat. no. FSGPD10) 1% E-Gel® 96 Agarose Gels (Thermofisher scientific, cat. no. G700801) E-gel Mother E-Base capable of running 96 samples (Thermofisher scientific, cat. no. EBM03) Temperature controlled shaker for 250 ml flasks and 50 ml tubes (MaxQ™ 6000 Shaker, Thermofisher scientific, cat. no. SHKE6000) Benchtop Centrifuge suitable for microplates and 50 ml tubes (Sorvall™ Legend™ XT/XF centrifuge, Thermofisher scientific, cat. no. 75-004-505) Plate shaker (Mixmate, Eppendorf, cat. no. 022674200) GENESYS™ 10S UV-Vis Spectrophotometer (Thermofisher scientific, cat. no. 840-208100) Vortexer (Thermofisher scientific, cat. no. 88880017TS) Centro XS3 Luminescence Microplate Reader (Berthold Technologies, cat. no. 46970) 1300 Series A2 Class II, Type A2 Bio Safety Cabinets (Thermofisher scientific, cat. no. 13-261-221) Optional: liquid handling robot with 96-well multichannel head and individual well cherry-picking capability (Tecan, cat. no. Fluent 780) Optional: BioRobot Universal System Robot with 96-well DNA miniprep capability (Qiagen, cat. no. 9001094) .. Custom DNA primers, 50 µM in a 96-well format, no PAGE purification required (See ; Sigma-Aldrich)

    Article Title: VERY LOW-DOSE (FEMTOMOLAR) 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN (TCDD) DISRUPTS STEROIDOGENIC ENZYME mRNAs AND STEROID SECRETION BY HUMAN LUTEINIZING GRANULOSA CELLS
    Article Snippet: .. All components were mixed directly into the corresponding wells of a hard-shell 96-well clear PCR plate (Bio-Rad, Hercules, CA; USA) and covered with a Microseal ‘B’ Film (Bio-Rad, Hercules, CA; USA) prior to placement in the thermal cycler. .. All Validated PrimePCR assays were verified to conform to MIQE [ ] standards established for RT-qPCR.

    Article Title: Genetic and environmental factors affecting the expression of α-gliadin canonical epitopes involved in celiac disease in a wide collection of spelt (Triticum aestivum ssp. spelta) cultivars and landraces
    Article Snippet: .. Two biological replicates of each sample, i.e. coming from 2 plants grown in the same plot, were loaded in triplicate in a Hard-Shell® 96-Well PCR skirted white plate and sealed with a Microseal® ‘B’ PCR Plate Sealing Film (Bio-Rad). .. PCR amplifications were performed by the C1000 Touch™ Thermal Cycler coupled to the CFX96™ Real-Time detection system (Bio-Rad).

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674) .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674)

    Real-time Polymerase Chain Reaction:

    Article Title: Cost-effective, high-throughput, single-haplotype iterative mapping and sequencing for complex genomic structures
    Article Snippet: .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674) .. AirPore Tape Sheets (Qiagen, cat. no. 19571) Eppendorf Twin.tec Plate (USA Scientific, cat. no. 4095–2624Q) Nunc 96 DeepWell Plate (Thermo Fisher Scientific, cat. no. 278743) TempPlate Semi-Skirt PCR Plate (USA Scientific, cat. no. 1402–9700) TempPlate XP PCR Sealing Film (USA Scientific, cat. no. 2972–2100) Adhesive PCR Plate Seals (Thermo Fisher Scientific, cat. no. AB0558) 96 microTUBE Plate (Covaris, cat. no. 520078) ▲ CRITICAL We find that the Covaris plate is essential to shear DNA evenly across all samples Costar Assay Plate 96-well (Corning, cat. no. 3797) 12-Strip 0.2 ml PCR Tubes (Neptune, cat. no. 3426.12.X) MicroAmp Fast Optical 96-Well Reaction Plate (Thermo Fisher Scientific, cat. no. 4346906) MicroAmp Optical Adhesive Film (Thermo Fisher Scientific, cat. no. 431197) Aluminum Adhesive Foil (Bio-rad, cat. no. MSF1001) Costar 50 mL reagent reservoirs (Corning, cat. no. 4870) 96-well Format Plate Magnet (Alpaqua, cat. no. 003011) 2100 Bioanalyzer (Agilent Technologies, cat. no. G2938A) 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, cat. no. 4351107) NanoDrop 1000 Spectrophotometer (Thermo Fisher Scientific, cat. no. ND 1000) SimpliAmp Thermal Cycler (Applied Biosystems, cat. no. ) Centrifuge 5810 R (Eppendorf, cat. no. 00267023) New Brunswick Innova 2300 (Eppendorf, cat. no. M1191–0022) E-Gel Precast Agarose Electrophoresis System (Thermo Fisher Scientific, cat. no. G6465) DynaMag-2 Magnet (Thermo Fisher Scientific, cat. no. 12321D) Vortex-Genie 2 (Scientific Industries, cat. no. SI-0236) LE220 Focused-ultrasonicator (Covaris, cat. no. 500219) Zephyr NGS Workstation (PerkinElmer, cat. no. 133750) ErgoOne 10–100 μl 12 Channel (USA Scientific, cat. no. 7108–1100) ErgoOne 30–300 μl 12 Channel (USA Scientific, cat. no. 7112–3300) VWR Signature Multichannel Electronic Pipettor (VWR, cat. no. 89000–674)

    Hood:

    Article Title: Isolation of human monoclonal antibodies from peripheral blood B cells
    Article Snippet: .. Flow cytometry cell sorter (BD Biosciences, cat. no. FACSAria II) Cell culture disposables: T-75 cell culture flasks (Thermo Scientific, cat. no. EW-01930-49), T-175 cell culture flasks (Thermo Scientific, cat. no. EW-01930-55), 5-ml polystyrene round-bottom tubes with cell-strainer caps (BD Falcon, cat. no. 352235), 15-ml polystyrene conical tubes (BD Falcon, cat. no. 352097) Stericup filter unit (Millipore, cat. no. SCGPU05RE) Screw cap microtube, 2 ml (Sarstedt, cat. no. SRS-72-694-006) Tissue culture plates, 384 wells (BD Falcon, cat. no. 353274) Guava PCA base system (EMD Millipore, cat. no. 0500-1090) Tissue culture hood (Thermo Scientific, cat. no. 1460) CO2 incubator set to 5% CO2 and 37 °C (Thermo Scientific, cat. no. 50116048) Zeiss ID 02 inverted microscope (American Instrument, cat. no. 4764D SCOPE) Cell culture centrifuges (Beckman Coulter, cat. no. BK392302) Microcentrifuge (Eppendorf, cat. no. 5430R) Microcentrifuge tubes Pipettes, 12 channels (Rainin, cat. no. L12-200XLS) Pipette tips (Rainin, cat. no. RT-L200F) Aluminized foil seal (Bio-Rad, cat. no. MSF-1001) Liquid nitrogen (Taylor Wharton, cat. no. 24K-kryos) Controlled-rate freezer (Thermo Scientific) Gammacell 1000 irradiator (Nordion International) P1000 pipette (Gilson) Milli-Q academic system (Millipore, cat. no. ZMQP60F01) ELISA plate reader (Bio-Tek Instruments) ..

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  • 92
    Bio-Rad microwells
    Designing <t>microwells</t> to hold cricket eggs. (A) Freshly laid cricket eggs were measured and their (B) lengths and (C) widths plotted ( n =98; scale bar: 500 µm). Based on the size distribution, we chose dimensions of 2930×570×650 µm (embryo length×width×height), which were values such that approximately 75% of eggs would fit into the troughs. In practice, because the wells are made of agarose, more than 95% of eggs fit into these wells. (D) Raised posts of those dimensions were formed by engraving an acrylic insert. 120 such posts were arranged in a grid. (E) Agarose microwells made using this insert, loaded with cricket eggs. (F,G) Example molds for an annelid worm and coqui frog, with microwell dimensions listed. Photos in F and G by Elaine Seaver (The Whitney Laboratory for Marine Bioscience) and Mara Laslo (Harvard University) respectively.
    Microwells, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microwells/product/Bio-Rad
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    microwells - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Bio-Rad microbead surface
    Detection of KIM-1 using mouse Rena-Stick in renal I/R model. KIM-1 in standard solutions or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the port in Rena-Stick. Results were read in 15min. (A) A concentration-dependent decrease in the band intensity was observed with a decrease in recombinant mouse KIM-1 protein standard concentration. (B) A visual increase in KIM-1 band intensity with an increase in bilateral ischemia time in mice subjected to renal I/R, whereas no KIM-1 positive band was observed when sham-operated mouse urine was analyzed. (C) The band intensity was quantified using a hand-held lateral-flow reader, and a standard curve was drawn by plotting the recombinant mouse KIM-1 concentration on x-axis and band intensity units on y-axis. (D) A positive correlation between KIM-1 values obtained by quantification of band intensity using the lateral-flow reader and values obtained by <t>microbead-based</t> assay in urines collected from bilateral I/R mice.
    Microbead Surface, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microbead surface/product/Bio-Rad
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    microbead surface - by Bioz Stars, 2020-07
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    92
    Bio-Rad microbeads
    Analysis of IgE-aptamer candidate binding after two rounds of aptamer isolation. IgE-coated <t>microbeads</t> were exposed to fluorescently labeled aptamer candidates, and the fluorescence intensity of the beads was measured. This signal corresponded to the surface concentration of DNA and was used to estimate a K D of 83.9 nM
    Microbeads, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microbeads/product/Bio-Rad
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    Price from $9.99 to $1999.99
    microbeads - by Bioz Stars, 2020-07
    92/100 stars
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    85
    Bio-Rad microscale thermophoresis histidine tagged full length ttf i
    Chromatin-specific termination at the homotypic cluster of <t>TTF-I.</t> (A) Overview of the murine rRNA gene and the location of the TTF-I binding sites. A homotypic cluster of TTF-I sites is located in the terminator region. The distances between TTF-I binding sites, their orientation and the gene promoter are indicated. A comparison of the TTF-I binding sites T 0 and the termination sites T 1 to T 10 is depicted. (B) Increasing amounts of TTF-IΔN348 were incubated with 50 nM of either a fluorescently labelled 30-mer oligonucleotide containing a Sal-box motif (T 2 ) or a control oligonucleotide of the same length. Protein-DNA interactions are quantified by microscale <t>thermophoresis.</t> Curve fitting with a Hill coefficient of 1 resulted in a K D of 500 nM+/−120 nM for the T 2 sequence. (C) Transcription reaction using the circular rDNA minigene plasmid pMr-SB containing a single termination site, a partially purified nuclear extract lacking most of the nuclear TTF-I (DEAE280), performed in the presence or absence of recombinant TTF-I. The positions of the long read-through and the terminated transcripts are indicated. (D) Transcription on free DNA and chromatin, using the pMrWT-T DNA containing the promoter with the TTF-I binding site T 0 and the full terminator with the 10 termination sites. DNA (lanes 1–8) and chromatin (lanes 9–16) were incubated with increasing concentrations of TTF-I as indicated and the DEAE280 extract. The position of the long, non-terminated read-through transcript (RT) and the terminated transcripts are indicated.
    Microscale Thermophoresis Histidine Tagged Full Length Ttf I, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microscale thermophoresis histidine tagged full length ttf i/product/Bio-Rad
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    microscale thermophoresis histidine tagged full length ttf i - by Bioz Stars, 2020-07
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    Image Search Results


    Designing microwells to hold cricket eggs. (A) Freshly laid cricket eggs were measured and their (B) lengths and (C) widths plotted ( n =98; scale bar: 500 µm). Based on the size distribution, we chose dimensions of 2930×570×650 µm (embryo length×width×height), which were values such that approximately 75% of eggs would fit into the troughs. In practice, because the wells are made of agarose, more than 95% of eggs fit into these wells. (D) Raised posts of those dimensions were formed by engraving an acrylic insert. 120 such posts were arranged in a grid. (E) Agarose microwells made using this insert, loaded with cricket eggs. (F,G) Example molds for an annelid worm and coqui frog, with microwell dimensions listed. Photos in F and G by Elaine Seaver (The Whitney Laboratory for Marine Bioscience) and Mara Laslo (Harvard University) respectively.

    Journal: Biology Open

    Article Title: High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system

    doi: 10.1242/bio.031260

    Figure Lengend Snippet: Designing microwells to hold cricket eggs. (A) Freshly laid cricket eggs were measured and their (B) lengths and (C) widths plotted ( n =98; scale bar: 500 µm). Based on the size distribution, we chose dimensions of 2930×570×650 µm (embryo length×width×height), which were values such that approximately 75% of eggs would fit into the troughs. In practice, because the wells are made of agarose, more than 95% of eggs fit into these wells. (D) Raised posts of those dimensions were formed by engraving an acrylic insert. 120 such posts were arranged in a grid. (E) Agarose microwells made using this insert, loaded with cricket eggs. (F,G) Example molds for an annelid worm and coqui frog, with microwell dimensions listed. Photos in F and G by Elaine Seaver (The Whitney Laboratory for Marine Bioscience) and Mara Laslo (Harvard University) respectively.

    Article Snippet: When making the microwells, agarose (Bio-Rad #1613101) was dissolved at 1.5% weight/volume (w/v) in distilled water (or 2% for firmer molds).

    Techniques:

    OMMAwell configurations for top-loaded microwells. (A) Configuration 1: Top loaded microwells for injecting or imaging with an upright microscope. (1) The mold insert (green) is inverted and connected to the slide (purple), which is placed into the upright platform (pink). (2) After the desired height is chosen, the pin (orange) is inserted. (3) Molten agarose is poured into a plastic petri dish and the mold assembly is lowered into it. (4) After the agarose sets, the mold insert is removed and eggs are placed into the wells, either individually with forceps or many at once by transferring the eggs in water with a cut plastic pipette. Excess water is removed by pipet and then wicked away with piece of lint-free lens paper. Then, 40–100 µl of molten low-melt agarose, kept at 42°C, is added to the wells to hold the eggs in place. Embryo positions are adjusted with plastic forceps. (5) When the low-melt agarose sets, the live-imaging medium is added to the dish. Right: Schematic of embryo in Configuration 1. (B) Configuration 2: Top loaded microwells for imaging with an inverted microscope. (1) 700 µl of agarose is pipetted into the middle of the glass-bottom dish. The insert and slide are lowered onto it, taking care not to trap bubbles. (2) Agarose sets, and then the insert and slide are gently removed. (3) Embryos are loaded into microwells, as described above. (4) Live-imaging medium is added. Right: Schematic of embryo in Configuration 2.

    Journal: Biology Open

    Article Title: High-throughput live-imaging of embryos in microwell arrays using a modular specimen mounting system

    doi: 10.1242/bio.031260

    Figure Lengend Snippet: OMMAwell configurations for top-loaded microwells. (A) Configuration 1: Top loaded microwells for injecting or imaging with an upright microscope. (1) The mold insert (green) is inverted and connected to the slide (purple), which is placed into the upright platform (pink). (2) After the desired height is chosen, the pin (orange) is inserted. (3) Molten agarose is poured into a plastic petri dish and the mold assembly is lowered into it. (4) After the agarose sets, the mold insert is removed and eggs are placed into the wells, either individually with forceps or many at once by transferring the eggs in water with a cut plastic pipette. Excess water is removed by pipet and then wicked away with piece of lint-free lens paper. Then, 40–100 µl of molten low-melt agarose, kept at 42°C, is added to the wells to hold the eggs in place. Embryo positions are adjusted with plastic forceps. (5) When the low-melt agarose sets, the live-imaging medium is added to the dish. Right: Schematic of embryo in Configuration 1. (B) Configuration 2: Top loaded microwells for imaging with an inverted microscope. (1) 700 µl of agarose is pipetted into the middle of the glass-bottom dish. The insert and slide are lowered onto it, taking care not to trap bubbles. (2) Agarose sets, and then the insert and slide are gently removed. (3) Embryos are loaded into microwells, as described above. (4) Live-imaging medium is added. Right: Schematic of embryo in Configuration 2.

    Article Snippet: When making the microwells, agarose (Bio-Rad #1613101) was dissolved at 1.5% weight/volume (w/v) in distilled water (or 2% for firmer molds).

    Techniques: Imaging, Microscopy, Transferring, Inverted Microscopy

    Detection of KIM-1 using mouse Rena-Stick in renal I/R model. KIM-1 in standard solutions or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the port in Rena-Stick. Results were read in 15min. (A) A concentration-dependent decrease in the band intensity was observed with a decrease in recombinant mouse KIM-1 protein standard concentration. (B) A visual increase in KIM-1 band intensity with an increase in bilateral ischemia time in mice subjected to renal I/R, whereas no KIM-1 positive band was observed when sham-operated mouse urine was analyzed. (C) The band intensity was quantified using a hand-held lateral-flow reader, and a standard curve was drawn by plotting the recombinant mouse KIM-1 concentration on x-axis and band intensity units on y-axis. (D) A positive correlation between KIM-1 values obtained by quantification of band intensity using the lateral-flow reader and values obtained by microbead-based assay in urines collected from bilateral I/R mice.

    Journal: Toxicological Sciences

    Article Title: Novel Assays for Detection of Urinary KIM-1 in Mouse Models of Kidney Injury

    doi: 10.1093/toxsci/kfs268

    Figure Lengend Snippet: Detection of KIM-1 using mouse Rena-Stick in renal I/R model. KIM-1 in standard solutions or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the port in Rena-Stick. Results were read in 15min. (A) A concentration-dependent decrease in the band intensity was observed with a decrease in recombinant mouse KIM-1 protein standard concentration. (B) A visual increase in KIM-1 band intensity with an increase in bilateral ischemia time in mice subjected to renal I/R, whereas no KIM-1 positive band was observed when sham-operated mouse urine was analyzed. (C) The band intensity was quantified using a hand-held lateral-flow reader, and a standard curve was drawn by plotting the recombinant mouse KIM-1 concentration on x-axis and band intensity units on y-axis. (D) A positive correlation between KIM-1 values obtained by quantification of band intensity using the lateral-flow reader and values obtained by microbead-based assay in urines collected from bilateral I/R mice.

    Article Snippet: The signal from the fluorochrome, which is directly proportional to the amount of antigen bound at the microbead surface, is captured using the Bio-Plex system (Bio-Rad).

    Techniques: Concentration Assay, Recombinant, Mouse Assay, Flow Cytometry

    Detection of KIM-1 using mouse Rena-Stick in AA model. KIM-1 in standard solution or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the sample port in Rena-Stick. Results were read in 15min. (A) A visual increase in KIM-1 band intensity at various time points after one dose of AA (5mg/kg). KIM-1 values obtained by quantification of band intensity using the lateral-flow reader were plotted versus values obtained by microbead-based assay in urines collected from AA-treated mice (B) and control mice (C).

    Journal: Toxicological Sciences

    Article Title: Novel Assays for Detection of Urinary KIM-1 in Mouse Models of Kidney Injury

    doi: 10.1093/toxsci/kfs268

    Figure Lengend Snippet: Detection of KIM-1 using mouse Rena-Stick in AA model. KIM-1 in standard solution or urine samples is measured by mixing 60 μl of sample with 60 μl of TRIS-buffered solution and adding the mix to the sample port in Rena-Stick. Results were read in 15min. (A) A visual increase in KIM-1 band intensity at various time points after one dose of AA (5mg/kg). KIM-1 values obtained by quantification of band intensity using the lateral-flow reader were plotted versus values obtained by microbead-based assay in urines collected from AA-treated mice (B) and control mice (C).

    Article Snippet: The signal from the fluorochrome, which is directly proportional to the amount of antigen bound at the microbead surface, is captured using the Bio-Plex system (Bio-Rad).

    Techniques: Flow Cytometry, Mouse Assay

    Analysis of IgE-aptamer candidate binding after two rounds of aptamer isolation. IgE-coated microbeads were exposed to fluorescently labeled aptamer candidates, and the fluorescence intensity of the beads was measured. This signal corresponded to the surface concentration of DNA and was used to estimate a K D of 83.9 nM

    Journal: Microfluidics and nanofluidics

    Article Title: Isolation of thermally sensitive protein-binding oligonucleotides on a microchip

    doi: 10.1007/s10404-015-1604-2

    Figure Lengend Snippet: Analysis of IgE-aptamer candidate binding after two rounds of aptamer isolation. IgE-coated microbeads were exposed to fluorescently labeled aptamer candidates, and the fluorescence intensity of the beads was measured. This signal corresponded to the surface concentration of DNA and was used to estimate a K D of 83.9 nM

    Article Snippet: Microbeads used for sequence selection were NHS-activated Sepha-rose beads obtained from Bio-Rad laboratories.

    Techniques: Binding Assay, Isolation, Labeling, Fluorescence, Concentration Assay

    Schematics of the aptamer isolation microchip. a Top view showing microchambers for affinity selection and PCR amplification. These chambers are connected via a serpentine diffusion-based mixing channel and integrated with thin-film resistive heaters and temperature sensors. b Cross-sectional view showing a weir-like restriction in which openings of 10 μm height retain microbeads roughly 100 μm in diameter

    Journal: Microfluidics and nanofluidics

    Article Title: Isolation of thermally sensitive protein-binding oligonucleotides on a microchip

    doi: 10.1007/s10404-015-1604-2

    Figure Lengend Snippet: Schematics of the aptamer isolation microchip. a Top view showing microchambers for affinity selection and PCR amplification. These chambers are connected via a serpentine diffusion-based mixing channel and integrated with thin-film resistive heaters and temperature sensors. b Cross-sectional view showing a weir-like restriction in which openings of 10 μm height retain microbeads roughly 100 μm in diameter

    Article Snippet: Microbeads used for sequence selection were NHS-activated Sepha-rose beads obtained from Bio-Rad laboratories.

    Techniques: Isolation, MicroChIP Assay, Selection, Polymerase Chain Reaction, Amplification, Diffusion-based Assay

    Fluorescent analysis of bead-based PCR amplification of candidate aptamers. Target-coated microbeads with strongly bound oligomers were washed multiple times, and the graph indicates the fluorescent intensity of microbeads following 25 cycles of PCR amplification of the eluent. The relative intensity of beads after each wash indicates that the starting DNA concentration was highest in the first eluted sample

    Journal: Microfluidics and nanofluidics

    Article Title: Isolation of thermally sensitive protein-binding oligonucleotides on a microchip

    doi: 10.1007/s10404-015-1604-2

    Figure Lengend Snippet: Fluorescent analysis of bead-based PCR amplification of candidate aptamers. Target-coated microbeads with strongly bound oligomers were washed multiple times, and the graph indicates the fluorescent intensity of microbeads following 25 cycles of PCR amplification of the eluent. The relative intensity of beads after each wash indicates that the starting DNA concentration was highest in the first eluted sample

    Article Snippet: Microbeads used for sequence selection were NHS-activated Sepha-rose beads obtained from Bio-Rad laboratories.

    Techniques: Polymerase Chain Reaction, Amplification, Concentration Assay

    Chromatin-specific termination at the homotypic cluster of TTF-I. (A) Overview of the murine rRNA gene and the location of the TTF-I binding sites. A homotypic cluster of TTF-I sites is located in the terminator region. The distances between TTF-I binding sites, their orientation and the gene promoter are indicated. A comparison of the TTF-I binding sites T 0 and the termination sites T 1 to T 10 is depicted. (B) Increasing amounts of TTF-IΔN348 were incubated with 50 nM of either a fluorescently labelled 30-mer oligonucleotide containing a Sal-box motif (T 2 ) or a control oligonucleotide of the same length. Protein-DNA interactions are quantified by microscale thermophoresis. Curve fitting with a Hill coefficient of 1 resulted in a K D of 500 nM+/−120 nM for the T 2 sequence. (C) Transcription reaction using the circular rDNA minigene plasmid pMr-SB containing a single termination site, a partially purified nuclear extract lacking most of the nuclear TTF-I (DEAE280), performed in the presence or absence of recombinant TTF-I. The positions of the long read-through and the terminated transcripts are indicated. (D) Transcription on free DNA and chromatin, using the pMrWT-T DNA containing the promoter with the TTF-I binding site T 0 and the full terminator with the 10 termination sites. DNA (lanes 1–8) and chromatin (lanes 9–16) were incubated with increasing concentrations of TTF-I as indicated and the DEAE280 extract. The position of the long, non-terminated read-through transcript (RT) and the terminated transcripts are indicated.

    Journal: PLoS Genetics

    Article Title: Chromatin-Specific Regulation of Mammalian rDNA Transcription by Clustered TTF-I Binding Sites

    doi: 10.1371/journal.pgen.1003786

    Figure Lengend Snippet: Chromatin-specific termination at the homotypic cluster of TTF-I. (A) Overview of the murine rRNA gene and the location of the TTF-I binding sites. A homotypic cluster of TTF-I sites is located in the terminator region. The distances between TTF-I binding sites, their orientation and the gene promoter are indicated. A comparison of the TTF-I binding sites T 0 and the termination sites T 1 to T 10 is depicted. (B) Increasing amounts of TTF-IΔN348 were incubated with 50 nM of either a fluorescently labelled 30-mer oligonucleotide containing a Sal-box motif (T 2 ) or a control oligonucleotide of the same length. Protein-DNA interactions are quantified by microscale thermophoresis. Curve fitting with a Hill coefficient of 1 resulted in a K D of 500 nM+/−120 nM for the T 2 sequence. (C) Transcription reaction using the circular rDNA minigene plasmid pMr-SB containing a single termination site, a partially purified nuclear extract lacking most of the nuclear TTF-I (DEAE280), performed in the presence or absence of recombinant TTF-I. The positions of the long read-through and the terminated transcripts are indicated. (D) Transcription on free DNA and chromatin, using the pMrWT-T DNA containing the promoter with the TTF-I binding site T 0 and the full terminator with the 10 termination sites. DNA (lanes 1–8) and chromatin (lanes 9–16) were incubated with increasing concentrations of TTF-I as indicated and the DEAE280 extract. The position of the long, non-terminated read-through transcript (RT) and the terminated transcripts are indicated.

    Article Snippet: Protein expression and microscale thermophoresis Histidine-tagged full-length TTF-I and the deletion mutants TTFΔN210 and TTFΔN348 were purified on a heparin column (Bio-Rad), followed by purification with Ni-NTA agarose according to the manufacturer's instructions (Qiagen).

    Techniques: Binding Assay, Incubation, Microscale Thermophoresis, Sequencing, Plasmid Preparation, Purification, Recombinant