microamp optical 96  (Thermo Fisher)


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    Structured Review

    Thermo Fisher microamp optical 96
    Microamp Optical 96, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/microamp optical 96/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    microamp optical 96 - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
    Article Snippet: .. Amplification of the cDNA was performed in the presence of gene-specific primers and the SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA) in MicroAmp Optical 96-well reaction plates with optical covers using an ABI Prism 7000 Sequence Detector (Applied Biosystems). ..

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems). .. The amplification protocol consisted of 50°C for 2 min, followed by 95°C for 2 min and then 40 cycles of 95°C for 5 s, 64°C for 15 s, and a 72°C extension for 10 s and an additional disassociation at 60°C for 15 s. Data were initially analyzed with Sequence Detector software (version 2.2.2) with a 0.2 threshold.

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: Each sample was amplified for mouse glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (forward: 5′-GAGCCAAACGGGTCATCATC-3′; reverse: 5′-CCTGCTTCACCACCTTCTTG-3′) to ensure equal cDNA input. .. For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA). .. A threshold cycle ( Ct value) was obtained for each amplification curve and a ΔCt value was first calculated by subtracting the Ct value for human cyclophilin A cDNA from the Ct value for each sample and transcript.

    Article Title: Next-Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains
    Article Snippet: Paragraph title: PCR amplification. ... Reactions were carried out in MicroAmp optical 96-well reaction plates (PN N801-0560; Life Technologies) and capped using MicroAmp 8-cap strips (PN 4323032; Life Technologies).

    Article Title: Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan(R) PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
    Article Snippet: FliC TaqMan® real-time PCR for ‘Dickeya solani’ Primers and TaqMan® MGB (5′-FAM/3′-BHQ1) probe (Life Technologies) specific for the fli C amplicon of ‘D. solani ’ ( ) were designed with Premier Biosoft's Allele ID version 7 software. .. The real-time PCR was performed in a 25 µl volume in a MicroAmp Optical 96 well reaction plate with Optical Caps (Life Technologies).

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ). ..

    Quantitative RT-PCR:

    Article Title: FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch
    Article Snippet: Paragraph title: qRT-PCR experiments ... 25 µL qPCR reactions were assembled in MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific).

    Article Title: CsrA modulates luxR transcript levels in Vibrio fischeri
    Article Snippet: Paragraph title: Quantitative RT-PCR analysis ... 2x SYBR Green master mix (Applied Biosystems) and H2 O were added to a final reaction volume of 50 µl/well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems).

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems). .. A human GAPDH probe was used for normalisation using the ΔCt method (4333764T; Applied Biosystems).

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: Paragraph title: Quantitative real-time RT-PCR ... PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ).

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: .. Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions. ..

    Gene Assay:

    Article Title: Amplification of the MET receptor drives resistance to anti-EGFR therapies in colorectal cancer
    Article Snippet: Furthermore, in order to exclude an entire chromosome 7 polysomy, the MAD1L1 gene (assay ID: hs00981515_cn), which is located on the p-arm of chromosome 7, was evaluated for CNV. .. PCR runs were performed using ABI MicroAmp Optical Fast 96-well Reaction Plates on an Applied Biosystems ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using 20 ng total gDNA as a template.

    Incubation:

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems). .. Gene expression assays were performed with commercially available Pre-made TaqMan Gene Expression assays for eight genes.

    Expressing:

    Article Title: Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
    Article Snippet: Amplification of the cDNA was performed in the presence of gene-specific primers and the SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA) in MicroAmp Optical 96-well reaction plates with optical covers using an ABI Prism 7000 Sequence Detector (Applied Biosystems). .. Relative quantification of gene expression data was carried out with the 2–ΔΔCT or comparative C T method ( ).

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: Real-time PCR was performed to check the expression of TLR2, TLR4, and TLR9 and also to check inducible nitric oxide synthase 2 (iNOS2). .. For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch
    Article Snippet: 25 µL qPCR reactions were assembled in MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific). .. FoxP2 expression was quantified relative to GAPDH and normalized to the GFP-injected animals using the 2-Δ ΔCT method ( ).

    Article Title: Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan(R) PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
    Article Snippet: The real-time PCR was performed in a 25 µl volume in a MicroAmp Optical 96 well reaction plate with Optical Caps (Life Technologies). .. Briefly, 2 µl DNA template was added to 12,5 µl Taqman Gene Expression master mix 2x, 0,5 µl of primers Dsf and Dsr (15 µM), 0,5 µl of probe Dsp (10 µM) and molecular-grade water up to a final volume of 25 µl.

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: For quantification of expression for candidate miRNA, RNA was reverse-transcribed with an Ncode miRNA first-strand cDNA synthesis kit (Invitrogen) according to the manufacturer-specified guidelines. .. Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions.

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems). .. Gene expression assays were performed with commercially available Pre-made TaqMan Gene Expression assays for eight genes.

    Transformation Assay:

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems). .. Gene copies in fecal samples were calculated from standard curves based on the log transformation of a known concentration versus the threshold cycle ( CT ).

    Sequencing:

    Article Title: Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
    Article Snippet: .. Amplification of the cDNA was performed in the presence of gene-specific primers and the SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA) in MicroAmp Optical 96-well reaction plates with optical covers using an ABI Prism 7000 Sequence Detector (Applied Biosystems). ..

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: The assays were performed with a 7900 HT Fast Real-Time Sequence Detector (Applied Biosystems). .. All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems).

    Article Title: Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan(R) PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
    Article Snippet: The real-time PCR was performed in a 25 µl volume in a MicroAmp Optical 96 well reaction plate with Optical Caps (Life Technologies). .. Amplification and signal detection was done in an ABI Prism 7900HT Sequence Detection System (Life Technologies).

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ). ..

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: .. Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions. ..

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems). .. Gene expression assays were performed with commercially available Pre-made TaqMan Gene Expression assays for eight genes.

    Concentration Assay:

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: Reaction mixtures (20 μl) contained 10 μl 2x SYBR Premix Ex Taq , 0.4 μl ROX reference dye (Takara Bio Inc., Shiga, Japan), 0.2 μM (final concentration) primers, and either 100 ng genomic DNA (fecal and water samples), a series of dilutions of plasmid DNA with a target insert with a log copy number of 8.8 to 1.8 (i.e., a copy number of 6.3 × 108 to about 63) per reaction mixture, or a series of dilutions of gull fecal DNA ranging from 60 to 6 × 10−7 ng per reaction mixture. .. All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems).

    Article Title: CsrA modulates luxR transcript levels in Vibrio fischeri
    Article Snippet: RNA was analyzed for integrity and concentration using a Bio-Rad Bioanalyzer, and converted to cDNA using an Applied Biosystems High-Capacity cDNA Reverse Transcription Kit. cDNA samples were stored at −20°C until use as templates in an Applied Biosystems 7300 Real-Time PCR system. .. 2x SYBR Green master mix (Applied Biosystems) and H2 O were added to a final reaction volume of 50 µl/well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems).

    Article Title: Next-Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains
    Article Snippet: Amplification was carried out in a 50-μl final volume reaction mixture containing 24.1 μl Ambion nuclease-free water (catalog no. AM 9932; Life Technologies), 5 μl 10× PCR buffer, 2 μl 50 mM MgCl2 (2 mM final concentration), 0.4 μl PCR nucleotide mix ultrapure deoxynucleoside triphosphates (dNTPs) (200 μM final concentration for each dNTP, PN 77119; USB, Santa Clara, CA), 0.5 μl Platinum Taq DNA polymerase (2.5-U final concentration), and 2 μl primer blend ( rpoB , katG , pncA , gyrA , or rrs gene; 0.4 μM final concentration for each primer). .. Reactions were carried out in MicroAmp optical 96-well reaction plates (PN N801-0560; Life Technologies) and capped using MicroAmp 8-cap strips (PN 4323032; Life Technologies).

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: The concentration of each sample was assessed using a Qubit Fluorometer and the Qubit RNA HS Assay Kit (Q32855; Invitrogen) following the kit's instructions. .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems).

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: To prepare cDNA, 1–3 μg total RNA was reverse-transcribed with random primers at a final concentration of 3 mM MgCl2 , 75 mM KCl, and 50 mM Tris-HCl (pH 8.3), 1 mM each of dNTPs, 10 units of RNase inhibitor, and 100 units of Superscript II Rnase H− reverse transcriptase (Invitrogen). .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems).

    TaqMan Copy Number Assay:

    Article Title: Amplification of the MET receptor drives resistance to anti-EGFR therapies in colorectal cancer
    Article Snippet: Quantitative PCR experiments for estimation of MET copy number variations were performed in triplicates using a Human TaqMan Copy Number Assay for MET (assay ID: hs01277655_cn) and the TaqMan Copy Number Reference Assay RNase P (Applied Biosystems, Foster City, CA). .. PCR runs were performed using ABI MicroAmp Optical Fast 96-well Reaction Plates on an Applied Biosystems ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using 20 ng total gDNA as a template.

    SYBR Green Assay:

    Article Title: Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
    Article Snippet: .. Amplification of the cDNA was performed in the presence of gene-specific primers and the SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA) in MicroAmp Optical 96-well reaction plates with optical covers using an ABI Prism 7000 Sequence Detector (Applied Biosystems). ..

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: The C. marimammalium PCR assay was also used in real-time assays, with SYBR green as the detection dye. .. All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems).

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: Real-time PCR was done in the Applied Biosystems 7500 fast real-time PCR using the power SYBR green PCR master mix. .. For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch
    Article Snippet: 25 µL qPCR reactions were assembled in MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific). .. Reaction components were 0.5 µL cDNA, 200 nM primers, 12.5 µL PowerUp SYBR Green Master Mix (ThermoFisher Scientific), and 10.75 uL nuclease-free water.

    Article Title: CsrA modulates luxR transcript levels in Vibrio fischeri
    Article Snippet: .. 2x SYBR Green master mix (Applied Biosystems) and H2 O were added to a final reaction volume of 50 µl/well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems). ..

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: The cDNA (1.0 μl) was used as a template in a 25-μl PCR reaction solution containing 10.5 μl, 1 μl 10 μM gene-specific primers, and 12.5 μl 1 × SYBR Green PCR master mix (SuperArray Bioscience, Frederick, MD). .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ).

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: .. Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions. ..

    Article Title: Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness
    Article Snippet: .. All reagents and chemicals were purchased from Sigma, except the following: FCS (0.05 ng/ml, 0.48 units/ml endotoxin) and recombinant murine IL-1β (Atlanta Biologicals, Norcross, GA); primer pairs (Qiagen, Alameda, CA); Sybr green PCR master mix and MicroAmp optical 96-well reaction plates (PE Applied Biosystems); TRIzol (Gibco BRL Life Technologies); superscript III RNase H- reverse transcriptase, 10 mM dNTP mix, and oligo dT primers (Invitrogen Life Technologies); RNasin Rnase inhibitor (Promega); mouse cannulas and cyanyoacrylate gel adhesive (Plastics One, Roanoke, VA); anti-mouse IL-1β (PM425B1), TNF-α (MM350C), IL-6 (MM600D), biotin-labeled anti-mouse IL-1β (MM425BB), TNF-α (MM350DB), IL-6 (MM600CB), horseradish peroxidase-conjugated streptavidin (n100), 3,3′,5,5′-tetramethyl-benzidine (TMB) substrate solution (n301), mouse IL-1β (SMIL1B), TNF-α (SMTNFN), and IL-6 (SMIL6) ELISA standard (Endogen, Woburn, MA); and Maxisorp-coated 96-well ELISA plates (Nalge Nunc, Rochester, NY). ..

    Northern Blot:

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: The same RNA isolated for northern blot analysis was also used for real-time PCR analysis. .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems).

    Generated:

    Article Title: Optimization of Molecular Approaches to Genogroup Neisseria meningitidis Carriage Isolates and Implications for Monitoring the Impact of New Serogroup B Vaccines
    Article Snippet: Real-Time PCR Assay and Analysis To evaluate the specificity of the primer-probe sets shown in , rt-PCR reactions were performed on strains from the proficiency panel under the following conditions: 25-μL reactions containing 400 nM of each primer, 200 nM of fluorescent probe, 12.5 μL of 2x QuantiTect Probe Kit (Qiagen) and 5 μL of DNA or lysate were assembled in MicroAmp Optical 96-Well Reaction Plates (Life Technologies). .. The analysis performed by the 7500 System SDS Software v1.3.1 generated the threshold cycle (Ct) for each reaction.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: CsrA modulates luxR transcript levels in Vibrio fischeri
    Article Snippet: 50 ng of cDNA was the template for the RT-PCR reaction, with primer concentrations of 250 µM. .. 2x SYBR Green master mix (Applied Biosystems) and H2 O were added to a final reaction volume of 50 µl/well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems).

    Article Title: Optimization of Molecular Approaches to Genogroup Neisseria meningitidis Carriage Isolates and Implications for Monitoring the Impact of New Serogroup B Vaccines
    Article Snippet: .. Real-Time PCR Assay and Analysis To evaluate the specificity of the primer-probe sets shown in , rt-PCR reactions were performed on strains from the proficiency panel under the following conditions: 25-μL reactions containing 400 nM of each primer, 200 nM of fluorescent probe, 12.5 μL of 2x QuantiTect Probe Kit (Qiagen) and 5 μL of DNA or lysate were assembled in MicroAmp Optical 96-Well Reaction Plates (Life Technologies). .. Reactions were run in an ABI Real-Time PCR System 7500 (Life Technologies) using these cycling parameters: 15 min at 95°C, followed by 40 cycles of 30 s at 95°C and 1 min at 60°C.

    Recombinant:

    Article Title: Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness
    Article Snippet: .. All reagents and chemicals were purchased from Sigma, except the following: FCS (0.05 ng/ml, 0.48 units/ml endotoxin) and recombinant murine IL-1β (Atlanta Biologicals, Norcross, GA); primer pairs (Qiagen, Alameda, CA); Sybr green PCR master mix and MicroAmp optical 96-well reaction plates (PE Applied Biosystems); TRIzol (Gibco BRL Life Technologies); superscript III RNase H- reverse transcriptase, 10 mM dNTP mix, and oligo dT primers (Invitrogen Life Technologies); RNasin Rnase inhibitor (Promega); mouse cannulas and cyanyoacrylate gel adhesive (Plastics One, Roanoke, VA); anti-mouse IL-1β (PM425B1), TNF-α (MM350C), IL-6 (MM600D), biotin-labeled anti-mouse IL-1β (MM425BB), TNF-α (MM350DB), IL-6 (MM600CB), horseradish peroxidase-conjugated streptavidin (n100), 3,3′,5,5′-tetramethyl-benzidine (TMB) substrate solution (n301), mouse IL-1β (SMIL1B), TNF-α (SMTNFN), and IL-6 (SMIL6) ELISA standard (Endogen, Woburn, MA); and Maxisorp-coated 96-well ELISA plates (Nalge Nunc, Rochester, NY). ..

    RNA HS Assay:

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: The concentration of each sample was assessed using a Qubit Fluorometer and the Qubit RNA HS Assay Kit (Q32855; Invitrogen) following the kit's instructions. .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems).

    Isolation:

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: Paragraph title: RNA isolation, RT, and RT-qPCR ... Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems).

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: The same RNA isolated for northern blot analysis was also used for real-time PCR analysis. .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems).

    Purification:

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: Total purified RNA integrity was checked by denaturing agarose gel electrophoresis and ethidium bromide staining. .. The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: For further purification, total RNA was subjected to 30 minutes TURBO DNase treatment (AM2239; Invitrogen) at 37°C following manufacturer’s instructions. .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems).

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ). ..

    Polymerase Chain Reaction:

    Article Title: Arabidopsis sterol carrier protein-2 is required for normal development of seeds and seedlings
    Article Snippet: .. Amplification of the cDNA was performed in the presence of gene-specific primers and the SYBR Green PCR master mix (Applied Biosystems, Foster City, CA, USA) in MicroAmp Optical 96-well reaction plates with optical covers using an ABI Prism 7000 Sequence Detector (Applied Biosystems). ..

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: Paragraph title: Conventional PCR and real-time assays. ... All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems).

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: Real-time PCR was done in the Applied Biosystems 7500 fast real-time PCR using the power SYBR green PCR master mix. .. For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: Amplification of the MET receptor drives resistance to anti-EGFR therapies in colorectal cancer
    Article Snippet: .. PCR runs were performed using ABI MicroAmp Optical Fast 96-well Reaction Plates on an Applied Biosystems ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using 20 ng total gDNA as a template. .. CNV of the target gene was calculated by the ABI SDS software 1.1 using relative quantification based on the ΔΔCt method and control samples as calibrators.

    Article Title: Next-Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains
    Article Snippet: Paragraph title: PCR amplification. ... Reactions were carried out in MicroAmp optical 96-well reaction plates (PN N801-0560; Life Technologies) and capped using MicroAmp 8-cap strips (PN 4323032; Life Technologies).

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems). .. A human GAPDH probe was used for normalisation using the ΔCt method (4333764T; Applied Biosystems).

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ). ..

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: .. Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions. ..

    Article Title: Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness
    Article Snippet: .. All reagents and chemicals were purchased from Sigma, except the following: FCS (0.05 ng/ml, 0.48 units/ml endotoxin) and recombinant murine IL-1β (Atlanta Biologicals, Norcross, GA); primer pairs (Qiagen, Alameda, CA); Sybr green PCR master mix and MicroAmp optical 96-well reaction plates (PE Applied Biosystems); TRIzol (Gibco BRL Life Technologies); superscript III RNase H- reverse transcriptase, 10 mM dNTP mix, and oligo dT primers (Invitrogen Life Technologies); RNasin Rnase inhibitor (Promega); mouse cannulas and cyanyoacrylate gel adhesive (Plastics One, Roanoke, VA); anti-mouse IL-1β (PM425B1), TNF-α (MM350C), IL-6 (MM600D), biotin-labeled anti-mouse IL-1β (MM425BB), TNF-α (MM350DB), IL-6 (MM600CB), horseradish peroxidase-conjugated streptavidin (n100), 3,3′,5,5′-tetramethyl-benzidine (TMB) substrate solution (n301), mouse IL-1β (SMIL1B), TNF-α (SMTNFN), and IL-6 (SMIL6) ELISA standard (Endogen, Woburn, MA); and Maxisorp-coated 96-well ELISA plates (Nalge Nunc, Rochester, NY). ..

    IA:

    Article Title: Next-Generation Ion Torrent Sequencing of Drug Resistance Mutations in Mycobacterium tuberculosis Strains
    Article Snippet: Reactions were carried out in MicroAmp optical 96-well reaction plates (PN N801-0560; Life Technologies) and capped using MicroAmp 8-cap strips (PN 4323032; Life Technologies). .. Electrophoretic separation of products was carried out for 60 min at 0.4 mV/cm2 in 1× Tris-borate-EDTA (TBE) buffer (catalog no. 1B70153; IBI Scientific, Peosta, IA).

    Mouse Assay:

    Article Title: Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness
    Article Snippet: All reagents and chemicals were purchased from Sigma, except the following: FCS (0.05 ng/ml, 0.48 units/ml endotoxin) and recombinant murine IL-1β (Atlanta Biologicals, Norcross, GA); primer pairs (Qiagen, Alameda, CA); Sybr green PCR master mix and MicroAmp optical 96-well reaction plates (PE Applied Biosystems); TRIzol (Gibco BRL Life Technologies); superscript III RNase H- reverse transcriptase, 10 mM dNTP mix, and oligo dT primers (Invitrogen Life Technologies); RNasin Rnase inhibitor (Promega); mouse cannulas and cyanyoacrylate gel adhesive (Plastics One, Roanoke, VA); anti-mouse IL-1β (PM425B1), TNF-α (MM350C), IL-6 (MM600D), biotin-labeled anti-mouse IL-1β (MM425BB), TNF-α (MM350DB), IL-6 (MM600CB), horseradish peroxidase-conjugated streptavidin (n100), 3,3′,5,5′-tetramethyl-benzidine (TMB) substrate solution (n301), mouse IL-1β (SMIL1B), TNF-α (SMTNFN), and IL-6 (SMIL6) ELISA standard (Endogen, Woburn, MA); and Maxisorp-coated 96-well ELISA plates (Nalge Nunc, Rochester, NY). .. B6.Cg- M +/+ Lepr db ( db /+) and B6.Cg-+ Lepr db /+ Lepr db ( db/db ) mice were bred in-house from mice purchased from The Jackson Laboratory.

    Plasmid Preparation:

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: Reaction mixtures (20 μl) contained 10 μl 2x SYBR Premix Ex Taq , 0.4 μl ROX reference dye (Takara Bio Inc., Shiga, Japan), 0.2 μM (final concentration) primers, and either 100 ng genomic DNA (fecal and water samples), a series of dilutions of plasmid DNA with a target insert with a log copy number of 8.8 to 1.8 (i.e., a copy number of 6.3 × 108 to about 63) per reaction mixture, or a series of dilutions of gull fecal DNA ranging from 60 to 6 × 10−7 ng per reaction mixture. .. All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems).

    Software:

    Article Title: Phylogenetic Diversity and Molecular Detection of Bacteria in Gull Feces
    Article Snippet: All reaction mixtures were prepared in triplicate in MicroAmp Optical 96-well reaction plates with MicroAmp Optical Caps (Applied Biosystems). .. The amplification protocol consisted of 50°C for 2 min, followed by 95°C for 2 min and then 40 cycles of 95°C for 5 s, 64°C for 15 s, and a 72°C extension for 10 s and an additional disassociation at 60°C for 15 s. Data were initially analyzed with Sequence Detector software (version 2.2.2) with a 0.2 threshold.

    Article Title: Amplification of the MET receptor drives resistance to anti-EGFR therapies in colorectal cancer
    Article Snippet: PCR runs were performed using ABI MicroAmp Optical Fast 96-well Reaction Plates on an Applied Biosystems ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using 20 ng total gDNA as a template. .. CNV of the target gene was calculated by the ABI SDS software 1.1 using relative quantification based on the ΔΔCt method and control samples as calibrators.

    Article Title: Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan(R) PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
    Article Snippet: FliC TaqMan® real-time PCR for ‘Dickeya solani’ Primers and TaqMan® MGB (5′-FAM/3′-BHQ1) probe (Life Technologies) specific for the fli C amplicon of ‘D. solani ’ ( ) were designed with Premier Biosoft's Allele ID version 7 software. .. The real-time PCR was performed in a 25 µl volume in a MicroAmp Optical 96 well reaction plate with Optical Caps (Life Technologies).

    Article Title: Optimization of Molecular Approaches to Genogroup Neisseria meningitidis Carriage Isolates and Implications for Monitoring the Impact of New Serogroup B Vaccines
    Article Snippet: Real-Time PCR Assay and Analysis To evaluate the specificity of the primer-probe sets shown in , rt-PCR reactions were performed on strains from the proficiency panel under the following conditions: 25-μL reactions containing 400 nM of each primer, 200 nM of fluorescent probe, 12.5 μL of 2x QuantiTect Probe Kit (Qiagen) and 5 μL of DNA or lysate were assembled in MicroAmp Optical 96-Well Reaction Plates (Life Technologies). .. The analysis performed by the 7500 System SDS Software v1.3.1 generated the threshold cycle (Ct) for each reaction.

    Real-time Polymerase Chain Reaction:

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: Paragraph title: Reverse transcriptase and real-time PCR. ... For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch
    Article Snippet: .. 25 µL qPCR reactions were assembled in MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific). .. Reaction components were 0.5 µL cDNA, 200 nM primers, 12.5 µL PowerUp SYBR Green Master Mix (ThermoFisher Scientific), and 10.75 uL nuclease-free water.

    Article Title: Amplification of the MET receptor drives resistance to anti-EGFR therapies in colorectal cancer
    Article Snippet: .. PCR runs were performed using ABI MicroAmp Optical Fast 96-well Reaction Plates on an Applied Biosystems ViiA 7 Real-Time PCR System (Applied Biosystems, Foster City, CA) using 20 ng total gDNA as a template. .. CNV of the target gene was calculated by the ABI SDS software 1.1 using relative quantification based on the ΔΔCt method and control samples as calibrators.

    Article Title: CsrA modulates luxR transcript levels in Vibrio fischeri
    Article Snippet: RNA was analyzed for integrity and concentration using a Bio-Rad Bioanalyzer, and converted to cDNA using an Applied Biosystems High-Capacity cDNA Reverse Transcription Kit. cDNA samples were stored at −20°C until use as templates in an Applied Biosystems 7300 Real-Time PCR system. .. 2x SYBR Green master mix (Applied Biosystems) and H2 O were added to a final reaction volume of 50 µl/well in a MicroAmp Optical 96-well reaction plate (Applied Biosystems).

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: .. The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA). .. Commercially available and pre-validated TaqMan® primer/probe sets were used as follows: cyclophilin A (4333763, RefSeq ), used as endogenous control for the target gene in each reaction, TNF alpha (Hs00174128_m1, RefSeq ), IL-6 (Hs00174131_m1, RefSeq ), IL-1β (Hs00174097_m1, RefSeq ), complement component 3 receptor 3 subunit (CD11b) (Hs01064804_m1, RefSeq ), insulin receptor substrate 1 (IRS-1) (Hs00178563_m1, RefSeq.), insulin receptor substrate 2 (IRS-2) (Hs00275843_s1, RefSeq.).

    Article Title: Sequence Diversity in the Dickeya fliC Gene: Phylogeny of the Dickeya Genus and TaqMan(R) PCR for 'D. solani', New Biovar 3 Variant on Potato in Europe
    Article Snippet: .. The real-time PCR was performed in a 25 µl volume in a MicroAmp Optical 96 well reaction plate with Optical Caps (Life Technologies). .. Briefly, 2 µl DNA template was added to 12,5 µl Taqman Gene Expression master mix 2x, 0,5 µl of primers Dsf and Dsr (15 µM), 0,5 µl of probe Dsp (10 µM) and molecular-grade water up to a final volume of 25 µl.

    Article Title: Cytosine-5 RNA methylation links protein synthesis to cell metabolism
    Article Snippet: .. Each RT quantitative PCR (RT-qPCR) reaction was set up in MicroAmp Optical 96-well plates (N8010560) using 1 μl cDNA, 5 μl TaqMan Fast Universal PCR Master Mix (4366073), 3.5 μl RNAse-free water, and 0.5 μl of a predesigned Nsun2 probe (Hs00214829_m1; Applied Biosystems). .. A human GAPDH probe was used for normalisation using the ΔCt method (4333764T; Applied Biosystems).

    Article Title: Optimization of Molecular Approaches to Genogroup Neisseria meningitidis Carriage Isolates and Implications for Monitoring the Impact of New Serogroup B Vaccines
    Article Snippet: .. Real-Time PCR Assay and Analysis To evaluate the specificity of the primer-probe sets shown in , rt-PCR reactions were performed on strains from the proficiency panel under the following conditions: 25-μL reactions containing 400 nM of each primer, 200 nM of fluorescent probe, 12.5 μL of 2x QuantiTect Probe Kit (Qiagen) and 5 μL of DNA or lysate were assembled in MicroAmp Optical 96-Well Reaction Plates (Life Technologies). .. Reactions were run in an ABI Real-Time PCR System 7500 (Life Technologies) using these cycling parameters: 15 min at 95°C, followed by 40 cycles of 30 s at 95°C and 1 min at 60°C.

    Article Title: Age-related changes in mesenchymal stem cells derived from rhesus macaque bone marrow
    Article Snippet: Paragraph title: Quantitative real-time PCR analysis for miRNA array ... Forward sequences used in this study were referenced from the Sanger miRNA database , and U6 was used as a normalizing control. qRT-PCR reactions were performed with the power SYBR green PCR master mix in a MicroAmp optical 96-well reaction plate with ABI PRISM® 7900HT sequence detector (Applied Biosystems) according to the manufacturer's instructions.

    Article Title: Fragile X-Related Proteins Regulate Mammalian Circadian Behavioral Rhythms
    Article Snippet: .. The reaction mixture was incubated at 37°C for 60 min. Real-time PCR was performed with the ABI PRISM 7000 Sequence Detector System (SDS) (Applied Biosystems) and MicroAmp optical 96-well plates (Applied Biosystems). .. Gene expression assays were performed with commercially available Pre-made TaqMan Gene Expression assays for eight genes.

    Agarose Gel Electrophoresis:

    Article Title: Successful Therapy of Murine Visceral Leishmaniasis with Astrakurkurone, a Triterpene Isolated from the Mushroom Astraeus hygrometricus, Involves the Induction of Protective Cell-Mediated Immunity and TLR9
    Article Snippet: The PCR products were run in a 1.2% agarose gel, and densitometry calculation was carried out. .. For the real-time reaction, 10 ng of cDNA template, 2 ng of forward primer, and 2 ng of reverse primer were used, and the reaction was performed on a 0.1-ml MicroAmp fast optical 96-well reaction plate (Applied Biosystems).

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: Total purified RNA integrity was checked by denaturing agarose gel electrophoresis and ethidium bromide staining. .. The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Inhibition of vagally mediated immune-to-brain signaling by vanadyl sulfate speeds recovery from sickness
    Article Snippet: .. All reagents and chemicals were purchased from Sigma, except the following: FCS (0.05 ng/ml, 0.48 units/ml endotoxin) and recombinant murine IL-1β (Atlanta Biologicals, Norcross, GA); primer pairs (Qiagen, Alameda, CA); Sybr green PCR master mix and MicroAmp optical 96-well reaction plates (PE Applied Biosystems); TRIzol (Gibco BRL Life Technologies); superscript III RNase H- reverse transcriptase, 10 mM dNTP mix, and oligo dT primers (Invitrogen Life Technologies); RNasin Rnase inhibitor (Promega); mouse cannulas and cyanyoacrylate gel adhesive (Plastics One, Roanoke, VA); anti-mouse IL-1β (PM425B1), TNF-α (MM350C), IL-6 (MM600D), biotin-labeled anti-mouse IL-1β (MM425BB), TNF-α (MM350DB), IL-6 (MM600CB), horseradish peroxidase-conjugated streptavidin (n100), 3,3′,5,5′-tetramethyl-benzidine (TMB) substrate solution (n301), mouse IL-1β (SMIL1B), TNF-α (SMTNFN), and IL-6 (SMIL6) ELISA standard (Endogen, Woburn, MA); and Maxisorp-coated 96-well ELISA plates (Nalge Nunc, Rochester, NY). ..

    Spectrophotometry:

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: RNA purity was determined by 260/280 absorbance ratios on a Nanodrop ND-1000 spectrophotometer (Thermo Fisher Scientific Inc. Waltham, MA). .. The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA).

    Activation Assay:

    Article Title: Involvement of AMP-activated protein kinase in beneficial effects of betaine on high-sucrose diet-induced hepatic steatosis
    Article Snippet: .. PCR amplification was conducted in MicroAmp Optical 96-well reaction plates (Applied Biosystems) on an Applied Biosystems PRISM 7000 sequence detection system under the following conditions: initial denaturation and enzyme activation for 10 min at 95°C, followed by 40 cycles of 95°C for 15 s and 60°C for 1 min. Each plate contained duplicate standards of purified PCR products of known template concentrations covering seven orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number vs. threshold cycle (CT ). ..

    Staining:

    Article Title: Insulin resistance is associated with specific gut microbiota in appendix samples from morbidly obese patients
    Article Snippet: Total purified RNA integrity was checked by denaturing agarose gel electrophoresis and ethidium bromide staining. .. The amplifications were performed using a MicroAmpH Optical 96-well reaction plate (Applied Biosystems, Foster City, CA) on an ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA).

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    Thermo Fisher microamp optical 96 well reaction plates
    Microamp Optical 96 Well Reaction Plates, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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