gene exp b2m hs00187842 m1  (Thermo Fisher)


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    Name:
    StepOnePlus Real Time PCR System
    Description:
    The StepOnePlus Real Time PCR System is a 96 well Real Time PCR instrument perfect for both first time and experienced users The StepOnePlus Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 4 color optical recording the StepOnePlus Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications We supply a Dell Laptop with the StepOnePlus Real Time PCR System Features of the StepOnePlus Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays• Sensitive 4 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageNew StepOne SoftwareThe StepOne Software included with the StepOnePlus Real Time PCR System runs on the Windows XP operating system and provides instrument control data collection and data analysis capabilities analysis software is also compatible with Windows 7 This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOnePlus Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 4 Color Fluorescence ReadingThe StepOnePlus Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE NED TAMRA and ROX dyes This cost effective 4 color 96 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus Real Time PCR System and StepOne Software The StepOnePlus System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Protein Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis as a standalone application • High Resolution Melting as a standalone application Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOnePlus System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOnePlus Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOnePlus Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Energy Efficiency and Space SavingThe StepOnePlus Real Time PCR System draws 38 less energy to process one sample plate when the instrument is in a heated state compared to the Bio Rad CFX96 Real Time PCR Detection System The StepOnePlus Real Time PCR System also has advanced temperature control through use of VeriFlex Blocks technology and has a footprint nearly 15 smaller than Bio Rad CFX96 which in today s crowded laboratories helps you use your laboratory space even more efficiently You also leave a smaller environmental footprint on the Earth Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOnePlus Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionAdvanced High Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOnePlus Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate
    Catalog Number:
    4376598
    Price:
    None
    Applications:
    Genotyping & Genomic Profiling|PCR & Real-Time PCR|Real Time PCR (qPCR)|Real Time PCR-Based Gene Expression Profiling|Real-Time PCR Instruments, Software & Calibration|Gene Expression Analysis & Genotyping|Genotyping Instruments, Software & Calibration|High Resolution Melting (HRM) Analysis
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    Instruments and Equipment
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    Structured Review

    Thermo Fisher gene exp b2m hs00187842 m1
    Effect of tocilizumab on the expression of C5aR1, C5aR2, and C3aR in NSTEMI patients. The effect of tocilizumab, a monoclonal antibody inhibiting interleukin 6 receptor (IL-6R), on the expression of the three complement anaphylatoxin receptors [C5aR1 (A) , C5aR2 (B) , and C3aR (C) ] was investigated in patients with non-ST-elevation myocardial infarction (NSTEMI). mRNA levels were quantified by qPCR and related to the reference gene <t>beta-2-microglobulin.</t> The tocilizumab group (gray bars, n = 28) and the placebo group (white bars, n = 32) are presented at four different time-points. Baseline levels show the receptor expression at inclusion, i.e., after hospital admission, before treatment was given. Follow-up time points were day 2 and 3, and 6 months. A group of healthy individuals ( n = 15) were included as controls. The qPCR results were quantified using the 2 −ΔΔCT method, normalized to reference genes and presented as fold change with the healthy controls as calibrator. Data are given as median and 95% CI. * P
    The StepOnePlus Real Time PCR System is a 96 well Real Time PCR instrument perfect for both first time and experienced users The StepOnePlus Real Time PCR System can be setup in a variety of configurations and comes ready to use out of the box with intuitive data analysis and instrument control software Utilizing robust LED based 4 color optical recording the StepOnePlus Real Time PCR System is designed to deliver precise quantitative Real Time PCR results for a variety of genomic research applications We supply a Dell Laptop with the StepOnePlus Real Time PCR System Features of the StepOnePlus Real Time PCR System include • Advanced software and instrumentation for performing a wide array of genomic assays• Sensitive 4 color optical LED recording system• Intuitive and robust software perfect for both first time and advanced users• Simple and flexible instrument set up and usageNew StepOne SoftwareThe StepOne Software included with the StepOnePlus Real Time PCR System runs on the Windows XP operating system and provides instrument control data collection and data analysis capabilities analysis software is also compatible with Windows 7 This latest version includes capabilities to collect melt curve data for High Resolution Melt HRM applications and the option to export in Real Time PCR Data Markup Language RDML for compatibility with MIQE guidelines Rapid and Simple Assay Set UpThis remarkably simple Real Time PCR system is designed with an LCD touchscreen and a user friendly interface that brings the power of genetic studies to researchers new to Real Time PCR Fig 1 The intuitive software and protocol wizards help guide new users through their Real Time PCR experiments The StepOnePlus Real Time PCR System also includes a quick start setup so that you start a run immediately and enter plate information at a later time Sensitive LED Based 4 Color Fluorescence ReadingThe StepOnePlus Real Time PCR System utilizes a long life LED based optical system that can record fluorescence from FAM SYBR Green VIC JOE NED TAMRA and ROX dyes This cost effective 4 color 96 well system delivers precise quantitative Real Time PCR results and saves data from all filters in every run without depending on a computer or plate setup It can discriminate between 2 populations of 5 000 and 10 000 template copies of a TaqMan assay with 99 7 confidence Compatible with Many Genomic Analysis TechniquesPerform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOnePlus Real Time PCR System and StepOne Software The StepOnePlus System supports many Real Time PCR applications including the following • SNP Genotyping• Gene Expression Analysis• MicroRNA Expression• Protein Expression• Translocation Analysis• Gene Detection• Viral Load AnalysisThe included StepOne Software supports a variety of analysis methods including the following • Standard Curve absolute quantitation • Relative Standard Curve• Comparative Ct ΔΔCt relative quantitation • Genotyping and Presence Absence• Melt Curve Analysis as a standalone application • High Resolution Melting as a standalone application Versatile Instrument Configuration OptionsThe ultra compact footprint of a StepOnePlus System can be installed in multiple distinct configurations providing unmatched flexibility and convenience that can allow a fit in any laboratory Fig 2 The StepOnePlus Real Time PCR System can be used via a PC with a PC connected to a Local Area Network LAN or as a stand alone instrument PC free Each StepOnePlus Real Time PCR System is factory calibrated for optical and thermal accuracy so simply remarkable Real Time PCR results can be obtained right out of the box Energy Efficiency and Space SavingThe StepOnePlus Real Time PCR System draws 38 less energy to process one sample plate when the instrument is in a heated state compared to the Bio Rad CFX96 Real Time PCR Detection System The StepOnePlus Real Time PCR System also has advanced temperature control through use of VeriFlex Blocks technology and has a footprint nearly 15 smaller than Bio Rad CFX96 which in today s crowded laboratories helps you use your laboratory space even more efficiently You also leave a smaller environmental footprint on the Earth Real Time Data Monitoring Dissemination and StorageThe system measures amplification as it occurs allowing you to monitor the progress of your experiment cycle by cycle either on the machine or remotely Your data is stored on the instrument itself and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive Data can also be conveniently exported as PowerPoint Excel and graphical image files Expanded Gene Expression CapabilitiesThe advanced software provided with the StepOnePlus Real Time PCR System now includes the powerful Gene Expression Study Package This software package allows for greater flexibility and accuracy in your gene expression assays through • Analysis of an unlimited number of plates in one study• Sorting of data by biological or technical replicate group• Use of multiple endogenous controls• Assay efficiency correctionAdvanced High Resolution Melting CapabilityNow with High Resolution Melt Software v3 0 you can perform post PCR sequence variation analysis with the StepOnePlus Real Time PCR System This separately purchased software simplifies set up by maintaining assay specific settings and accepts pasted plate layout information directly from Excel HRM Software v3 0 also has an improved clustering algorithm for increased sensitivity and accuracy and the ability to conduct separate analyses of multiple assays run on a single plate
    https://www.bioz.com/result/gene exp b2m hs00187842 m1/product/Thermo Fisher
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    Images

    1) Product Images from "IL-6 Receptor Inhibition by Tocilizumab Attenuated Expression of C5a Receptor 1 and 2 in Non-ST-Elevation Myocardial Infarction"

    Article Title: IL-6 Receptor Inhibition by Tocilizumab Attenuated Expression of C5a Receptor 1 and 2 in Non-ST-Elevation Myocardial Infarction

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.02035

    Effect of tocilizumab on the expression of C5aR1, C5aR2, and C3aR in NSTEMI patients. The effect of tocilizumab, a monoclonal antibody inhibiting interleukin 6 receptor (IL-6R), on the expression of the three complement anaphylatoxin receptors [C5aR1 (A) , C5aR2 (B) , and C3aR (C) ] was investigated in patients with non-ST-elevation myocardial infarction (NSTEMI). mRNA levels were quantified by qPCR and related to the reference gene beta-2-microglobulin. The tocilizumab group (gray bars, n = 28) and the placebo group (white bars, n = 32) are presented at four different time-points. Baseline levels show the receptor expression at inclusion, i.e., after hospital admission, before treatment was given. Follow-up time points were day 2 and 3, and 6 months. A group of healthy individuals ( n = 15) were included as controls. The qPCR results were quantified using the 2 −ΔΔCT method, normalized to reference genes and presented as fold change with the healthy controls as calibrator. Data are given as median and 95% CI. * P
    Figure Legend Snippet: Effect of tocilizumab on the expression of C5aR1, C5aR2, and C3aR in NSTEMI patients. The effect of tocilizumab, a monoclonal antibody inhibiting interleukin 6 receptor (IL-6R), on the expression of the three complement anaphylatoxin receptors [C5aR1 (A) , C5aR2 (B) , and C3aR (C) ] was investigated in patients with non-ST-elevation myocardial infarction (NSTEMI). mRNA levels were quantified by qPCR and related to the reference gene beta-2-microglobulin. The tocilizumab group (gray bars, n = 28) and the placebo group (white bars, n = 32) are presented at four different time-points. Baseline levels show the receptor expression at inclusion, i.e., after hospital admission, before treatment was given. Follow-up time points were day 2 and 3, and 6 months. A group of healthy individuals ( n = 15) were included as controls. The qPCR results were quantified using the 2 −ΔΔCT method, normalized to reference genes and presented as fold change with the healthy controls as calibrator. Data are given as median and 95% CI. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    2) Product Images from "Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome"

    Article Title: Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome

    Journal: Endocrinology

    doi: 10.1210/en.2015-1413

    Dysregulated expressions of proteins involved in cellular redox status in the CD34 negative -enriched omental ASC fraction. A, Increased expression of NOX2 and NOX5 in Non-MS subjects and correlation of NOX2 mRNA with AP (R = −0.627, P = .039) and osteonectin mRNA (R = −0.682, P = .021). B, mRNA expression levels of SOD1 and SOD3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Correlation of SOD1 mRNA was performed with HOMA-IR (R = 0.673, P = .023) and serum insulin (R = 0.618, P = .043). NOX2, NOX5, SOD1, and SOD3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P
    Figure Legend Snippet: Dysregulated expressions of proteins involved in cellular redox status in the CD34 negative -enriched omental ASC fraction. A, Increased expression of NOX2 and NOX5 in Non-MS subjects and correlation of NOX2 mRNA with AP (R = −0.627, P = .039) and osteonectin mRNA (R = −0.682, P = .021). B, mRNA expression levels of SOD1 and SOD3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Correlation of SOD1 mRNA was performed with HOMA-IR (R = 0.673, P = .023) and serum insulin (R = 0.618, P = .043). NOX2, NOX5, SOD1, and SOD3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P

    Techniques Used: Expressing, Mass Spectrometry, Quantitative RT-PCR, MANN-WHITNEY

    3) Product Images from "Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR"

    Article Title: Monitoring Antigen-Specific T Cell Responses Using Real-Time PCR

    Journal: Methods in molecular biology (Clifton, N.J.)

    doi: 10.1007/978-1-4939-1158-5_5

    Representative qRT-PCR amplification and melting curve plots for the human IFN-γ- and CD8-specific amplicons. ( a ) CD8 and IFN-γ were amplified from cDNA of a normal HLA-A2+ human donor through qRT-PCR using the StepOnePlus™ Real-Time
    Figure Legend Snippet: Representative qRT-PCR amplification and melting curve plots for the human IFN-γ- and CD8-specific amplicons. ( a ) CD8 and IFN-γ were amplified from cDNA of a normal HLA-A2+ human donor through qRT-PCR using the StepOnePlus™ Real-Time

    Techniques Used: Quantitative RT-PCR, Amplification

    4) Product Images from "NNK, a Tobacco-Specific Carcinogen, Inhibits the Expression of Lysyl Oxidase, a Tumor Suppressor"

    Article Title: NNK, a Tobacco-Specific Carcinogen, Inhibits the Expression of Lysyl Oxidase, a Tumor Suppressor

    Journal: International Journal of Environmental Research and Public Health

    doi: 10.3390/ijerph120100064

    NNK inhibition of LOX steady–state mRNA levels in treated cells as revealed by reverse transcription (RT)-PCR and agarose gel electrophoresis ( A ) and quantitative real-time-PCR ( B ). ( A ) Total RNA (1 µg) was extracted from growth-arrested control and treated cells using Trizol reagent. Reverse-transcription cDNA was produced using the SuperScript first-strand synthesis system. LOX and GAPDH (an internal control) cDNA fragments were amplified by PCR and analyzed on a 2.2% agarose gel. Densities of PCR-amplified gene fragments on the gel as described here and below were measured with the 1D Scan software. ( B ) The real-time PCR was performed by the GeneAmpR 5700 Sequence Detection System (SDS) using reverse-transcription DNA as a template. PCR products were monitored by fluorescence from the TaqMan probes for LOX and GAPDH (an internal control) and analyzed using the GeneAmp 5700 SDS software. Data shown are the mean ± SD ( n = 3). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 compared with the control.
    Figure Legend Snippet: NNK inhibition of LOX steady–state mRNA levels in treated cells as revealed by reverse transcription (RT)-PCR and agarose gel electrophoresis ( A ) and quantitative real-time-PCR ( B ). ( A ) Total RNA (1 µg) was extracted from growth-arrested control and treated cells using Trizol reagent. Reverse-transcription cDNA was produced using the SuperScript first-strand synthesis system. LOX and GAPDH (an internal control) cDNA fragments were amplified by PCR and analyzed on a 2.2% agarose gel. Densities of PCR-amplified gene fragments on the gel as described here and below were measured with the 1D Scan software. ( B ) The real-time PCR was performed by the GeneAmpR 5700 Sequence Detection System (SDS) using reverse-transcription DNA as a template. PCR products were monitored by fluorescence from the TaqMan probes for LOX and GAPDH (an internal control) and analyzed using the GeneAmp 5700 SDS software. Data shown are the mean ± SD ( n = 3). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 compared with the control.

    Techniques Used: Inhibition, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Real-time Polymerase Chain Reaction, Produced, Amplification, Polymerase Chain Reaction, Software, Sequencing, Fluorescence

    5) Product Images from "Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe"

    Article Title: Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe

    Journal: Genome Biology

    doi: 10.1186/s13059-015-0788-9

    Comparing replication origin usage in syntenic  L. mexicana  and  L. major  chromosomes that have undergone fusion or fission.  a  Graphs show replication origin localisation, evaluated by MFAseq, in  L. mexicana  ( Lmx ) chromosomes 8 and 20, which are syntenic with  L. major  ( Lmj ) chromosomes 29 and 8 and chromosomes 36 and 20, respectively (chromosome sizes are denoted in 0.25 Mb intervals). Blocks of synteny are boxed and their relative orientation indicated; the representation of early S/G2 DNA sequence read depth ratios ( L. mexicana green ,  L. major blue ) and coding sequence organisation are as detailed in Fig.   1  and the approximate location of the origin or syntenic non-origin loci is shown by  solid vertical lines  and  dotted vertical lines , respectively. (Figures S4 and S5 in Additional file   1  show MFAseq for all  L. mexicana  chromosomes and a genome-wide comparison with  L. major .)  b  Validation of replication origin activity in the  L. mexicana  and  L. major  chromosomes (shown in ( a )) by quantitative PCR, which was performed at a number of loci predicted to display origin activity in  L. major  and syntenic with  L. mexicana . At each locus the relative quantity of S phase ( black ) and G2 phase ( red ) DNA is shown: G2 values at each loci are set at 1, and the S phase samples are shown as a proportion of that value ( vertical lines  indicate standard deviation from at least three experimental repeats); for comparison, the MFAseq data (from ( a )) is shown in the background, and the right-hand synteny regions are distinguished from the left hand regions using  dotted lines  and  solid lines , respectively. Positions of the quantitative PCR loci in each chromosome are shown in megabases (x-axes)
    Figure Legend Snippet: Comparing replication origin usage in syntenic L. mexicana and L. major chromosomes that have undergone fusion or fission. a Graphs show replication origin localisation, evaluated by MFAseq, in L. mexicana ( Lmx ) chromosomes 8 and 20, which are syntenic with L. major ( Lmj ) chromosomes 29 and 8 and chromosomes 36 and 20, respectively (chromosome sizes are denoted in 0.25 Mb intervals). Blocks of synteny are boxed and their relative orientation indicated; the representation of early S/G2 DNA sequence read depth ratios ( L. mexicana green , L. major blue ) and coding sequence organisation are as detailed in Fig.  1 and the approximate location of the origin or syntenic non-origin loci is shown by solid vertical lines and dotted vertical lines , respectively. (Figures S4 and S5 in Additional file 1 show MFAseq for all L. mexicana chromosomes and a genome-wide comparison with L. major .) b Validation of replication origin activity in the L. mexicana and L. major chromosomes (shown in ( a )) by quantitative PCR, which was performed at a number of loci predicted to display origin activity in L. major and syntenic with L. mexicana . At each locus the relative quantity of S phase ( black ) and G2 phase ( red ) DNA is shown: G2 values at each loci are set at 1, and the S phase samples are shown as a proportion of that value ( vertical lines indicate standard deviation from at least three experimental repeats); for comparison, the MFAseq data (from ( a )) is shown in the background, and the right-hand synteny regions are distinguished from the left hand regions using dotted lines and solid lines , respectively. Positions of the quantitative PCR loci in each chromosome are shown in megabases (x-axes)

    Techniques Used: Sequencing, Genome Wide, Activity Assay, Real-time Polymerase Chain Reaction, Standard Deviation

    6) Product Images from "Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease"

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease

    Journal: Antiviral research

    doi: 10.1016/j.antiviral.2017.12.018

    Inhibition of the NS2B-NS3 interactions and protease activity (A)  Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66  to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.
    Figure Legend Snippet: Inhibition of the NS2B-NS3 interactions and protease activity (A) Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66 to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.

    Techniques Used: Inhibition, Activity Assay, Binding Assay

    Inhibition of ZIKV in cells relevant to ZIKV ( A ) qRT-PCR analysis of inhibition of viral RNA from ZIKV infected A549 cells by erythrosin B.  (B)  Immunofluorescence assay (IFA) of inhibition of viral protein expression by erythrosin B, using pan-flavivirus anti-E 4G2 antibody (green) (ATCC). Nuclei (blue) was stained in all IFA assays by the Hoechst stain solution.  (C) ). At 48 h post infection, cells were washed, harvested, and protected by protease inhibitor cocktail prior to lysis by SDS-PAGE loading buffer. Upon incubation at 95 °C for 10 min, sample was subjected to Western blot analysis with anti-ZIKV NS3 (GTX133309, GeneTex, Inc.) and anti-GAPDH (CB1001, EMD Millipore) as primary antibodies. ( D,E ) Viral plaque reduction assay for ZIKV-infected HPECs ( D ) and iPSC-derived hNPCs ( E ) by erythrosin B. ***, p
    Figure Legend Snippet: Inhibition of ZIKV in cells relevant to ZIKV ( A ) qRT-PCR analysis of inhibition of viral RNA from ZIKV infected A549 cells by erythrosin B. (B) Immunofluorescence assay (IFA) of inhibition of viral protein expression by erythrosin B, using pan-flavivirus anti-E 4G2 antibody (green) (ATCC). Nuclei (blue) was stained in all IFA assays by the Hoechst stain solution. (C) ). At 48 h post infection, cells were washed, harvested, and protected by protease inhibitor cocktail prior to lysis by SDS-PAGE loading buffer. Upon incubation at 95 °C for 10 min, sample was subjected to Western blot analysis with anti-ZIKV NS3 (GTX133309, GeneTex, Inc.) and anti-GAPDH (CB1001, EMD Millipore) as primary antibodies. ( D,E ) Viral plaque reduction assay for ZIKV-infected HPECs ( D ) and iPSC-derived hNPCs ( E ) by erythrosin B. ***, p

    Techniques Used: Inhibition, Quantitative RT-PCR, Infection, Immunofluorescence, Expressing, Staining, Protease Inhibitor, Lysis, SDS Page, Incubation, Western Blot, Derivative Assay

    Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.
    Figure Legend Snippet: Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.

    Techniques Used: Inhibition

    7) Product Images from "Cyclin-dependent kinase 4 signaling acts as a molecular switch between syngenic differentiation and neural transdifferentiation in human mesenchymal stem cells"

    Article Title: Cyclin-dependent kinase 4 signaling acts as a molecular switch between syngenic differentiation and neural transdifferentiation in human mesenchymal stem cells

    Journal: Cell Cycle

    doi: 10.4161/cc.23308

    Figure 4. Inhibition of CDK4 activity reduces the differentiation potential of adipose-derived MSCs into syngenic lineages. ( A ) hAD-MSCs were treated with CDK4 inhibitor (2 μM, Otava Ltd.) or control DMSO and then cultured in the adipogenic differentiation media. At 2 wk post-treatment, the MSCs showed the formation of lipid vacuoles, which were stained with Oil Red O. ( B ) hAD-MSCs were treated with CDK4 inhibitor (2 μM and 10 μM) or control DMSO for 24 h. The hAD-MSCs treated with the high concentration of CDK4 inhibitor showed a typical neural cell appearance. ( C and D ) hAD-MSCs were treated with CDK4 inhibitor (2 μM) or control DMSO and further cultured in the absence or presence of adipogenic differentiation media for 2 wk. The relative rate of adipogenic differentiation, the lipid vacuoles of which were stained with Oil Red O, was measured at OD500 nm ( C ). Adipogenic marker transcripts (Adiponectin, Lipoprotein lipase and PPARγ2) were amplified by RT-PCR from the MSCs as above. GAPDH mRNA was utilized as an internal control for RT-PCR ( D ).
    Figure Legend Snippet: Figure 4. Inhibition of CDK4 activity reduces the differentiation potential of adipose-derived MSCs into syngenic lineages. ( A ) hAD-MSCs were treated with CDK4 inhibitor (2 μM, Otava Ltd.) or control DMSO and then cultured in the adipogenic differentiation media. At 2 wk post-treatment, the MSCs showed the formation of lipid vacuoles, which were stained with Oil Red O. ( B ) hAD-MSCs were treated with CDK4 inhibitor (2 μM and 10 μM) or control DMSO for 24 h. The hAD-MSCs treated with the high concentration of CDK4 inhibitor showed a typical neural cell appearance. ( C and D ) hAD-MSCs were treated with CDK4 inhibitor (2 μM) or control DMSO and further cultured in the absence or presence of adipogenic differentiation media for 2 wk. The relative rate of adipogenic differentiation, the lipid vacuoles of which were stained with Oil Red O, was measured at OD500 nm ( C ). Adipogenic marker transcripts (Adiponectin, Lipoprotein lipase and PPARγ2) were amplified by RT-PCR from the MSCs as above. GAPDH mRNA was utilized as an internal control for RT-PCR ( D ).

    Techniques Used: Inhibition, Activity Assay, Derivative Assay, Cell Culture, Staining, Concentration Assay, Relative Rate, Marker, Amplification, Reverse Transcription Polymerase Chain Reaction

    Figure 1. Adipose-derived MSCs selectively commit to transdifferentiate into neural cells following CDK inhibition. ( A ) Phase contrast image of human adipose-derived MSCs (hAD-MSCs). hAD-MSCs were isolated from the fatty portion of liposuction aspirates. ( B ) hAD-MSCs were labeled with FITC- or PE-coupled antibodies specific for CD34, CD44, CD45, CD49d, CD73 (not shown), CD90 and CD106 antibodies or immunoglobulin isotype control. The surface phenotype was analyzed by FACS. Colored histograms illustrate the control immunoglobulin and open histograms illustrate the specific antibodies, as indicated. Isolated MSCs are positive for the MSC markers (CD44, CD49d and CD90), whereas they are negative for hematopoietic stem cell markers (CD34, CD45 and CD106). Blue-colored shadows represent the isotype control and green represent the staining against each specified antibodies. ( C ) Adipogenic differentiation resulted in the formation of lipid vacuoles, which were stained with Oil Red O. Osteogenic differentiation showed a highly enriched extracellular matrix stained with von Kossa. Neurogenic differentiation led to a typical neural cell appearance (i.e., a condensed cell body, spherical and refractile), and were immunostained with anti-MAP2 antibody. ( D ) hAD-MSCs were isolated from six different donors, and characterized as described in ( B and C ). hAD-MSCs were treated with 10 μM CDK4i for 24 h and digitally imaged. Treatment with CDK4i induced the morphologic neural transdifferentiation with high efficiency. ( E ) Quantified comparison of cell death and neural transdifferentiation of hAD-MSCs following CDK inhibitor (PurA, 25 μM and CDK4i, 10 μM) treatment. The rate of differentiating hAD-MSCs was randomly counted from 10 different microscopic images (X200) per sample. Averages were obtained from six different MSCs and donors. ( F ) hAD-MSCs were treated with Purvalanol A (PurA, 25 μM) and CDK4i (10 μM). Cells were harvested, stained with propidium iodide and analyzed by flow cytometry to determine their DNA contents. ( G ) Phase contrast images of human fetal white matter progenitor/precursor cells (NPCs), human U251 glioblastoma cells and human AD-MSCs. NPCs were cultured in DMEM (NPC) or DMEM supplemented with ITSFn medium (NPC w/ ITSFn). ( H ) RT-PCR analysis for neural progenitor/precursor cell markers (NCAM, Msi1, and Sox2), a marker for stem cell multi-potency (Nestin) and mesenchymal stem cell surface markers (CD90 and CD105). GAPDH mRNA was utilized as a control.
    Figure Legend Snippet: Figure 1. Adipose-derived MSCs selectively commit to transdifferentiate into neural cells following CDK inhibition. ( A ) Phase contrast image of human adipose-derived MSCs (hAD-MSCs). hAD-MSCs were isolated from the fatty portion of liposuction aspirates. ( B ) hAD-MSCs were labeled with FITC- or PE-coupled antibodies specific for CD34, CD44, CD45, CD49d, CD73 (not shown), CD90 and CD106 antibodies or immunoglobulin isotype control. The surface phenotype was analyzed by FACS. Colored histograms illustrate the control immunoglobulin and open histograms illustrate the specific antibodies, as indicated. Isolated MSCs are positive for the MSC markers (CD44, CD49d and CD90), whereas they are negative for hematopoietic stem cell markers (CD34, CD45 and CD106). Blue-colored shadows represent the isotype control and green represent the staining against each specified antibodies. ( C ) Adipogenic differentiation resulted in the formation of lipid vacuoles, which were stained with Oil Red O. Osteogenic differentiation showed a highly enriched extracellular matrix stained with von Kossa. Neurogenic differentiation led to a typical neural cell appearance (i.e., a condensed cell body, spherical and refractile), and were immunostained with anti-MAP2 antibody. ( D ) hAD-MSCs were isolated from six different donors, and characterized as described in ( B and C ). hAD-MSCs were treated with 10 μM CDK4i for 24 h and digitally imaged. Treatment with CDK4i induced the morphologic neural transdifferentiation with high efficiency. ( E ) Quantified comparison of cell death and neural transdifferentiation of hAD-MSCs following CDK inhibitor (PurA, 25 μM and CDK4i, 10 μM) treatment. The rate of differentiating hAD-MSCs was randomly counted from 10 different microscopic images (X200) per sample. Averages were obtained from six different MSCs and donors. ( F ) hAD-MSCs were treated with Purvalanol A (PurA, 25 μM) and CDK4i (10 μM). Cells were harvested, stained with propidium iodide and analyzed by flow cytometry to determine their DNA contents. ( G ) Phase contrast images of human fetal white matter progenitor/precursor cells (NPCs), human U251 glioblastoma cells and human AD-MSCs. NPCs were cultured in DMEM (NPC) or DMEM supplemented with ITSFn medium (NPC w/ ITSFn). ( H ) RT-PCR analysis for neural progenitor/precursor cell markers (NCAM, Msi1, and Sox2), a marker for stem cell multi-potency (Nestin) and mesenchymal stem cell surface markers (CD90 and CD105). GAPDH mRNA was utilized as a control.

    Techniques Used: Derivative Assay, Inhibition, Isolation, Labeling, FACS, Staining, Flow Cytometry, Cytometry, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Marker

    Figure 3. Targeted inhibition of CDK4 expression induces the neural transdifferentiation. ( A ) To deplete the endogenous CDK1, CDK2 and CDK4, ADSCs were transduced with recombinant adenovirus expressing GFP fused-shCDK1 (rAd-GFP-shCDK1), -shCDK2 (rAd-GFP-shCDK2) or -shCDK4 (rAd-GFP-shCDK4), respectively, in conjugation with HP4-PTD. Forty-eight hours post-transduction, cell lysates were prepared and immunoblotted with anti-CDK1, anti-CDK2, anti-CDK4 and anti-actin antibodies. ( B ) hAD-MSCs were transduced with rAd-GFP [as a control (data not shown)], rAd-GFP-shCDK1, rAd-GFP-shCDK2 or rAd-GFP-shCDK4 and rAd-H2B-RFP to visualize DNA. Transduced hAD-MSCs were cultured in normal culture medium and digitally monitored by time-lapse microscopy. Images were taken at 6 min intervals, showing the false-colored GFP and RFP emissions. The red-colored arrows indicate the neural cells induced by CDK4 depletion. ( C ) Neural sub-type marker transcripts (Nestin, S100, NL3 and GFAP) were amplified by RT-PCR from hAD-MSCs transduced with rAd-GFP or rAd-GFP-shCDK4. GAPDH mRNA was utilized as an internal control for RT-PCR.
    Figure Legend Snippet: Figure 3. Targeted inhibition of CDK4 expression induces the neural transdifferentiation. ( A ) To deplete the endogenous CDK1, CDK2 and CDK4, ADSCs were transduced with recombinant adenovirus expressing GFP fused-shCDK1 (rAd-GFP-shCDK1), -shCDK2 (rAd-GFP-shCDK2) or -shCDK4 (rAd-GFP-shCDK4), respectively, in conjugation with HP4-PTD. Forty-eight hours post-transduction, cell lysates were prepared and immunoblotted with anti-CDK1, anti-CDK2, anti-CDK4 and anti-actin antibodies. ( B ) hAD-MSCs were transduced with rAd-GFP [as a control (data not shown)], rAd-GFP-shCDK1, rAd-GFP-shCDK2 or rAd-GFP-shCDK4 and rAd-H2B-RFP to visualize DNA. Transduced hAD-MSCs were cultured in normal culture medium and digitally monitored by time-lapse microscopy. Images were taken at 6 min intervals, showing the false-colored GFP and RFP emissions. The red-colored arrows indicate the neural cells induced by CDK4 depletion. ( C ) Neural sub-type marker transcripts (Nestin, S100, NL3 and GFAP) were amplified by RT-PCR from hAD-MSCs transduced with rAd-GFP or rAd-GFP-shCDK4. GAPDH mRNA was utilized as an internal control for RT-PCR.

    Techniques Used: Inhibition, Expressing, Transduction, Recombinant, Conjugation Assay, Cell Culture, Time-lapse Microscopy, Marker, Amplification, Reverse Transcription Polymerase Chain Reaction

    8) Product Images from "Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome"

    Article Title: Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome

    Journal: Endocrinology

    doi: 10.1210/en.2015-1413

    Expression of fibrotic proteins in the CD34 negative -enriched omental ASC fraction. A, Correlation between mRNA expression levels of NOX5 and Col1a1 (R = 0.555; P = .077), Col3a1 (R = 0.445; P = .170), Col5a1 (R = 0.627; P = .039), and Col6a3 (R = 0.645; P = .032). B, Correlation between HOMA-IR and Col1a1 (R = 0.600; P = .051), Col3a1 (R = 0.773; P = .005), Col5a1 (R = 0.445; P = .170), and Col6a3 (R = 0.045; P = .894). C, mRNA expression levels of Col1a1, Col3a1, Col5a1, and Col6a3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Col1a1, Col3a1, Col5a1, and Col6a3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P
    Figure Legend Snippet: Expression of fibrotic proteins in the CD34 negative -enriched omental ASC fraction. A, Correlation between mRNA expression levels of NOX5 and Col1a1 (R = 0.555; P = .077), Col3a1 (R = 0.445; P = .170), Col5a1 (R = 0.627; P = .039), and Col6a3 (R = 0.645; P = .032). B, Correlation between HOMA-IR and Col1a1 (R = 0.600; P = .051), Col3a1 (R = 0.773; P = .005), Col5a1 (R = 0.445; P = .170), and Col6a3 (R = 0.045; P = .894). C, mRNA expression levels of Col1a1, Col3a1, Col5a1, and Col6a3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Col1a1, Col3a1, Col5a1, and Col6a3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P

    Techniques Used: Expressing, Quantitative RT-PCR, Mass Spectrometry, MANN-WHITNEY

    9) Product Images from "The Effects of Knockdown of Rho-Associated Kinase 1 and Zipper-Interacting Protein Kinase on Gene Expression and Function in Cultured Human Arterial Smooth Muscle Cells"

    Article Title: The Effects of Knockdown of Rho-Associated Kinase 1 and Zipper-Interacting Protein Kinase on Gene Expression and Function in Cultured Human Arterial Smooth Muscle Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0116969

    Effects of ROCK1 and ZIPK knockdown on IL-6 and IL-1β mRNA expression and IL-6 protein secretion. (A) CASMCs were transfected with siRNA to ZIPK or ROCK1 or with negative control siRNA. Cells were lysed 48 h later for qRT-PCR to quantify IL-6 and IL-1β mRNA levels. Values represent means ± SEM ( n = 7 for IL-6 and n = 6 for IL-1β). *significantly different from control (p
    Figure Legend Snippet: Effects of ROCK1 and ZIPK knockdown on IL-6 and IL-1β mRNA expression and IL-6 protein secretion. (A) CASMCs were transfected with siRNA to ZIPK or ROCK1 or with negative control siRNA. Cells were lysed 48 h later for qRT-PCR to quantify IL-6 and IL-1β mRNA levels. Values represent means ± SEM ( n = 7 for IL-6 and n = 6 for IL-1β). *significantly different from control (p

    Techniques Used: Expressing, Transfection, Negative Control, Quantitative RT-PCR

    10) Product Images from "Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression"

    Article Title: Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5161-13.2014

    Neuronal gp130 was essential for Trpa1 mRNA expression in DRG. A , TaqMan mRNA analysis revealed significantly lower Trpa1 mRNA levels in DRG explants and primary neuronal cultures of SNS-gp130 −/− mice (DRG from thoracic and lumbar levels). B , Trpa1 mRNA expression was significantly reduced in lumbar DRG explants from SNS-gp130 −/− mice compared with gp130 fl/fl and SNS-Cre , which were not significantly different. Furthermore, Trpv1 and Trpv4 mRNA levels were similar between gp130 fl/fl and SNS-gp130 −/− mice. C , Trpa1 mRNA expression of lumbar DRG explants within the first 14 postnatal days. In SNS-gp130 −/− mice, Trpa1 mRNA expression was already reduced at P1–P14. Expression levels are normalized to adult gp130 fl/fl mice. * p
    Figure Legend Snippet: Neuronal gp130 was essential for Trpa1 mRNA expression in DRG. A , TaqMan mRNA analysis revealed significantly lower Trpa1 mRNA levels in DRG explants and primary neuronal cultures of SNS-gp130 −/− mice (DRG from thoracic and lumbar levels). B , Trpa1 mRNA expression was significantly reduced in lumbar DRG explants from SNS-gp130 −/− mice compared with gp130 fl/fl and SNS-Cre , which were not significantly different. Furthermore, Trpv1 and Trpv4 mRNA levels were similar between gp130 fl/fl and SNS-gp130 −/− mice. C , Trpa1 mRNA expression of lumbar DRG explants within the first 14 postnatal days. In SNS-gp130 −/− mice, Trpa1 mRNA expression was already reduced at P1–P14. Expression levels are normalized to adult gp130 fl/fl mice. * p

    Techniques Used: Expressing, Mouse Assay

    11) Product Images from "Effect of interleukin (IL)-35 on IL-17 expression and production by human CD4+ T cells"

    Article Title: Effect of interleukin (IL)-35 on IL-17 expression and production by human CD4+ T cells

    Journal: PeerJ

    doi: 10.7717/peerj.2999

    Effect of IL-35 on expression of IL17A , RORA and RORC mRNA. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 2 h. IL17A (A), RORA (B) and RORC (C) mRNA expression was determined and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P
    Figure Legend Snippet: Effect of IL-35 on expression of IL17A , RORA and RORC mRNA. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 2 h. IL17A (A), RORA (B) and RORC (C) mRNA expression was determined and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P

    Techniques Used: Expressing, Cell Differentiation, Cell Culture

    Effect of IL-35 on IL-17A production in Th17 cell. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 24 h and IL-17A production in the culture supernatant was evaluated. Values are shown as the mean ± standard error of three independent experiments. ∗ P
    Figure Legend Snippet: Effect of IL-35 on IL-17A production in Th17 cell. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 24 h and IL-17A production in the culture supernatant was evaluated. Values are shown as the mean ± standard error of three independent experiments. ∗ P

    Techniques Used: Cell Differentiation, Cell Culture

    Percentage of IL-17A, IFN- γ and Foxp3 expressing CD4 + T cells in peripheral blood from healthy volunteers and CP patients. Representative dot plots of IL-17A or IFN- γ positive CD4 + T cells and IL-17A or Foxp3 positive CD4 + T cells in the peripheral blood from healthy volunteers and CP patients. Dot plots are representative of three individuals per group.
    Figure Legend Snippet: Percentage of IL-17A, IFN- γ and Foxp3 expressing CD4 + T cells in peripheral blood from healthy volunteers and CP patients. Representative dot plots of IL-17A or IFN- γ positive CD4 + T cells and IL-17A or Foxp3 positive CD4 + T cells in the peripheral blood from healthy volunteers and CP patients. Dot plots are representative of three individuals per group.

    Techniques Used: Expressing

    12) Product Images from "Effect of interleukin (IL)-35 on IL-17 expression and production by human CD4+ T cells"

    Article Title: Effect of interleukin (IL)-35 on IL-17 expression and production by human CD4+ T cells

    Journal: PeerJ

    doi: 10.7717/peerj.2999

    Effect of IL-35 on expression of IL17A , RORA and RORC mRNA. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 2 h. IL17A (A), RORA (B) and RORC (C) mRNA expression was determined and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P
    Figure Legend Snippet: Effect of IL-35 on expression of IL17A , RORA and RORC mRNA. After Th17 cell differentiation by cytokine cocktail, cells were cultured with or without rIL-35 (1 ng/mL) for 2 h. IL17A (A), RORA (B) and RORC (C) mRNA expression was determined and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P

    Techniques Used: Expressing, Cell Differentiation, Cell Culture

    Expression levels of RORA and RORC mRNA in two Th17 cell differentiation strategies. CD4 + T cells in peripheral blood from three healthy volunteers were incubated with a cytokine cocktail or rIL-23 for Th17 cell differentiation. RORA (A) and RORC (B) mRNA levels were evaluated and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P
    Figure Legend Snippet: Expression levels of RORA and RORC mRNA in two Th17 cell differentiation strategies. CD4 + T cells in peripheral blood from three healthy volunteers were incubated with a cytokine cocktail or rIL-23 for Th17 cell differentiation. RORA (A) and RORC (B) mRNA levels were evaluated and normalized to ACTB . Values are shown as the mean ± standard error of three independent experiments. ∗∗ P

    Techniques Used: Expressing, Cell Differentiation, Incubation

    13) Product Images from "Serum microRNA-125a-5p as a potential biomarker of HCV-associated hepatocellular carcinoma"

    Article Title: Serum microRNA-125a-5p as a potential biomarker of HCV-associated hepatocellular carcinoma

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10385

    Expression of serum miR-125a-5p in patients with hepatitis C virus-associated liver diseases. (A) Relative expression levels. The expression levels of miR-125a-5p were significantly upregulated in patients with early and advanced stage HCC, compared with patients with CH or LC. There was no significant difference between patients with CH and LC. The relative expression levels of miR-125a-5p was determined by calculating 2 −ΔΔCq , where ΔCq=Cq miR-125a-5p -Cq cel-miR-39 , ΔΔCq=ΔCq-mean ΔCq of patients with CH. The horizontal lines represent the mean relative expression levels. *P
    Figure Legend Snippet: Expression of serum miR-125a-5p in patients with hepatitis C virus-associated liver diseases. (A) Relative expression levels. The expression levels of miR-125a-5p were significantly upregulated in patients with early and advanced stage HCC, compared with patients with CH or LC. There was no significant difference between patients with CH and LC. The relative expression levels of miR-125a-5p was determined by calculating 2 −ΔΔCq , where ΔCq=Cq miR-125a-5p -Cq cel-miR-39 , ΔΔCq=ΔCq-mean ΔCq of patients with CH. The horizontal lines represent the mean relative expression levels. *P

    Techniques Used: Expressing

    14) Product Images from "Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages"

    Article Title: Pseudomonas aeruginosa N-3-oxo-dodecanoyl-homoserine Lactone Elicits Changes in Cell Volume, Morphology, and AQP9 Characteristics in Macrophages

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2016.00032

    AQP9 intensity profiles across the leading and trailing regions in macrophages . The experiment, measurements and the quantifications of AQP9 intensity profiles over the cell in the direction of polarization were performed as shown by white arrow in Figure 4A . AQP9 intensity difference in the first 40% of cell leading region (left row) and last 40% of cell trailing region (right row) in macrophages after 3O-C 12 -HSL-treatments for 1, 4, and 24 h are shown in (A), (B) , and (C) , respectively. Values are the mean ± SE. Significant differences are indicated with * when P
    Figure Legend Snippet: AQP9 intensity profiles across the leading and trailing regions in macrophages . The experiment, measurements and the quantifications of AQP9 intensity profiles over the cell in the direction of polarization were performed as shown by white arrow in Figure 4A . AQP9 intensity difference in the first 40% of cell leading region (left row) and last 40% of cell trailing region (right row) in macrophages after 3O-C 12 -HSL-treatments for 1, 4, and 24 h are shown in (A), (B) , and (C) , respectively. Values are the mean ± SE. Significant differences are indicated with * when P

    Techniques Used:

    Nanoscale visualization of AQP9 dynamic in lamellipodial area in 3O-C 12 -HSL stimulated macrophages . Macrophages were stimulated with 10 μM 3O-C 12 -HSL for 1, 4, or 24 h, or 0.02% DMSO as a vehicle control (Control), stained for AQP9 (green) and analyzed with STED nanoscopy. (A) Main panels, bar 5 μm. (B) Inserts, image size is 15.5 × 6.2 μm. (C) AQP9 fluorescence intensity profiles measured as indicated by colored arrows in (A, B) in the direction from lamellipodial area to the edge of macrophages on the representative images.
    Figure Legend Snippet: Nanoscale visualization of AQP9 dynamic in lamellipodial area in 3O-C 12 -HSL stimulated macrophages . Macrophages were stimulated with 10 μM 3O-C 12 -HSL for 1, 4, or 24 h, or 0.02% DMSO as a vehicle control (Control), stained for AQP9 (green) and analyzed with STED nanoscopy. (A) Main panels, bar 5 μm. (B) Inserts, image size is 15.5 × 6.2 μm. (C) AQP9 fluorescence intensity profiles measured as indicated by colored arrows in (A, B) in the direction from lamellipodial area to the edge of macrophages on the representative images.

    Techniques Used: Staining, Fluorescence

    Nanoscale imaging of AQP9 architecture in 3O-C 12 -HSL-stimulated macrophages. (A) Macrophages were stimulated with 10μM 3O-C 12 -HSL for 1, 4 or 24 h, or 0.02% DMSO as a vehicle control (Control), stained for AQP9 (green) and analyzed with STED nanoscopy. The two left panels show the images after Huygens deconvolution (gold) with improved visualization quality. Bar 5 μm. (B) AQP9 fluorescence intensity profiles over the cells, measured as indicated by colored arrows in (A) in the direction from nuclear to lamellipodial area on the representative images.
    Figure Legend Snippet: Nanoscale imaging of AQP9 architecture in 3O-C 12 -HSL-stimulated macrophages. (A) Macrophages were stimulated with 10μM 3O-C 12 -HSL for 1, 4 or 24 h, or 0.02% DMSO as a vehicle control (Control), stained for AQP9 (green) and analyzed with STED nanoscopy. The two left panels show the images after Huygens deconvolution (gold) with improved visualization quality. Bar 5 μm. (B) AQP9 fluorescence intensity profiles over the cells, measured as indicated by colored arrows in (A) in the direction from nuclear to lamellipodial area on the representative images.

    Techniques Used: Imaging, Staining, Fluorescence

    3O-C 12 -HSL increases the expression of AQP9 in macrophages. (A) Changes in AQP9 mRNA levels of macrophages. Cells were treated either with diluent (Control) or with 10, 50, and 100 μM 3O-C 12 -HSL for 1, 4, and 24 h, and qPCR was performed using GAPDH as a reference gene. Two-way ANOVA suggested that the concentration but not the different times of observation primarily affected the expression rate. Significant difference is indicated with * when P
    Figure Legend Snippet: 3O-C 12 -HSL increases the expression of AQP9 in macrophages. (A) Changes in AQP9 mRNA levels of macrophages. Cells were treated either with diluent (Control) or with 10, 50, and 100 μM 3O-C 12 -HSL for 1, 4, and 24 h, and qPCR was performed using GAPDH as a reference gene. Two-way ANOVA suggested that the concentration but not the different times of observation primarily affected the expression rate. Significant difference is indicated with * when P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Concentration Assay

    Visualization of AQP9 cellular distribution in 3O-C 12 -HSL-stimulated macrophages. (A) Macrophages were either untreated (Untreated control), or treated with diluent (Control), or stimulated with 10, 50, and 100 μM 3O-C 12 -HSL for 1, 4, or 24 h, stained for AQP9 (red) and analyzed by LSCM. Staining with secondary antibody (Sec. control) as a control. The data are from one of six independent experiments. Bar 10 μm. (B) Quantification of AQP9 immunofluorescence intensity measured as a total integrated intensity density of whole macrophage area. Columns represent the means ± SE. Significant differences are indicated with * when P
    Figure Legend Snippet: Visualization of AQP9 cellular distribution in 3O-C 12 -HSL-stimulated macrophages. (A) Macrophages were either untreated (Untreated control), or treated with diluent (Control), or stimulated with 10, 50, and 100 μM 3O-C 12 -HSL for 1, 4, or 24 h, stained for AQP9 (red) and analyzed by LSCM. Staining with secondary antibody (Sec. control) as a control. The data are from one of six independent experiments. Bar 10 μm. (B) Quantification of AQP9 immunofluorescence intensity measured as a total integrated intensity density of whole macrophage area. Columns represent the means ± SE. Significant differences are indicated with * when P

    Techniques Used: Staining, Size-exclusion Chromatography, Immunofluorescence

    Effect of 3O-C 12 -HSL on AQP9-related cell size of macrophages . Cell were either treated with diluent 0.02% DMSO (Control) for 24 h, or stimulated with 10, 50, and 100 μM 3O-C 12 -HSL for 24 h, stained for AQP9, analyzed by LSCM as shown in Figure 4A . (A) Quantification of AQP9-related cell area. (B) Quantification of the approximate cell length of AQP9-stained macrophages (measured as indicated by white arrow in Figure 4A in the direction of polarization). Columns represent the means ± SE. Data from at least four different experiments performed on separate days from six different donors, and at least 100 cells in total per condition. Significant differences are indicated with * when P
    Figure Legend Snippet: Effect of 3O-C 12 -HSL on AQP9-related cell size of macrophages . Cell were either treated with diluent 0.02% DMSO (Control) for 24 h, or stimulated with 10, 50, and 100 μM 3O-C 12 -HSL for 24 h, stained for AQP9, analyzed by LSCM as shown in Figure 4A . (A) Quantification of AQP9-related cell area. (B) Quantification of the approximate cell length of AQP9-stained macrophages (measured as indicated by white arrow in Figure 4A in the direction of polarization). Columns represent the means ± SE. Data from at least four different experiments performed on separate days from six different donors, and at least 100 cells in total per condition. Significant differences are indicated with * when P

    Techniques Used: Staining

    15) Product Images from "Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples"

    Article Title: Evaluation of a single-tube fluorogenic RT-PCR assay for detection of bovine respiratory syncytial virus in clinical samples

    Journal: Journal of Virological Methods

    doi: 10.1016/j.jviromet.2004.09.016

    BRSV nested PCR results from analyses on the same RNA templates as described in Fig. 2 . The products of PCR 1 (a) and PCR 2 (b) are shown by gel electrophoresis. Predicted product size is 711 bp for PCR 1 and 481 bp for PCR 2. The detection limit is 10 4 times diluted sample (number 4 on gel b). Numbers 1–9 correspond to the dilutions from 10 to 10 9 ; number 10 is negative control.
    Figure Legend Snippet: BRSV nested PCR results from analyses on the same RNA templates as described in Fig. 2 . The products of PCR 1 (a) and PCR 2 (b) are shown by gel electrophoresis. Predicted product size is 711 bp for PCR 1 and 481 bp for PCR 2. The detection limit is 10 4 times diluted sample (number 4 on gel b). Numbers 1–9 correspond to the dilutions from 10 to 10 9 ; number 10 is negative control.

    Techniques Used: Nested PCR, Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Negative Control

    Standard curve for calculation of BRSV-fRT PCR efficiency. The curve is generated by analyses of a 10-fold dilution series of an RNA template (dilutions 1:1 to 10 3 , five replicates of each dilution).
    Figure Legend Snippet: Standard curve for calculation of BRSV-fRT PCR efficiency. The curve is generated by analyses of a 10-fold dilution series of an RNA template (dilutions 1:1 to 10 3 , five replicates of each dilution).

    Techniques Used: Polymerase Chain Reaction, Generated

    16) Product Images from "Serum microRNA-125a-5p as a potential biomarker of HCV-associated hepatocellular carcinoma"

    Article Title: Serum microRNA-125a-5p as a potential biomarker of HCV-associated hepatocellular carcinoma

    Journal: Oncology Letters

    doi: 10.3892/ol.2019.10385

    Expression of serum miR-125a-5p in patients with hepatitis C virus-associated liver diseases. (A) Relative expression levels. The expression levels of miR-125a-5p were significantly upregulated in patients with early and advanced stage HCC, compared with patients with CH or LC. There was no significant difference between patients with CH and LC. The relative expression levels of miR-125a-5p was determined by calculating 2 −ΔΔCq , where ΔCq=Cq miR-125a-5p -Cq cel-miR-39 , ΔΔCq=ΔCq-mean ΔCq of patients with CH. The horizontal lines represent the mean relative expression levels. *P
    Figure Legend Snippet: Expression of serum miR-125a-5p in patients with hepatitis C virus-associated liver diseases. (A) Relative expression levels. The expression levels of miR-125a-5p were significantly upregulated in patients with early and advanced stage HCC, compared with patients with CH or LC. There was no significant difference between patients with CH and LC. The relative expression levels of miR-125a-5p was determined by calculating 2 −ΔΔCq , where ΔCq=Cq miR-125a-5p -Cq cel-miR-39 , ΔΔCq=ΔCq-mean ΔCq of patients with CH. The horizontal lines represent the mean relative expression levels. *P

    Techniques Used: Expressing

    17) Product Images from "Expression of BMI-1 and Mel-18 in breast tissue - a diagnostic marker in patients with breast cancer"

    Article Title: Expression of BMI-1 and Mel-18 in breast tissue - a diagnostic marker in patients with breast cancer

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-10-686

    Quantitative real-time PCR shows the same inverse relationship between the transcriptional levels of Bmi-1 and Mel-18 as the microarray data . The qPCR results were calculated using the comparative C T -method (-ΔΔC T ) while the microarray results are given as the difference in expression of the two genes (log 2 (expression of Bmi-1/expression of Mel-18)).
    Figure Legend Snippet: Quantitative real-time PCR shows the same inverse relationship between the transcriptional levels of Bmi-1 and Mel-18 as the microarray data . The qPCR results were calculated using the comparative C T -method (-ΔΔC T ) while the microarray results are given as the difference in expression of the two genes (log 2 (expression of Bmi-1/expression of Mel-18)).

    Techniques Used: Real-time Polymerase Chain Reaction, Microarray, Expressing

    18) Product Images from "Aging and Replicative Senescence Have Related Effects on Human Stem and Progenitor Cells"

    Article Title: Aging and Replicative Senescence Have Related Effects on Human Stem and Progenitor Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0005846

    QRT-PCR validation of differential gene expression. Age associated gene expression changes in MSC were validated by quantitative RT-PCR. 10 genes were selected that have been differentially expressed in microarray data (A): regeneration associated muscle protease (RAMP); maternally expressed 3 (MEG3); interleukin 13 receptor, alpha 2 (IL13RA2); S100 calcium binding protein A4 (S100A4) and triggering receptor expressed on myeloid cells 1 (TREM1) were age-induced. Homeobox B3 (HOXB3) and Homeobox B7 (HOXB7); midline 1 (MID1); small nuclear RNA activating complex, polypeptide 5 (SNAPC5) and peroxisome proliferator-activated receptor gamma (PPARG) were age-repressed. Furthermore, we have validated age associated changes in HPC for 9 genes (B): S100 calcium binding protein A10 (S100A10); vimentin (VIM); myeloid-associated differentiation marker (MYADM); pim-1 oncogene (PIM1) and annexin A2 (ANXA2) were age-induced. Timeless interacting protein (TIPIN); myosin regulatory light chain interacting protein (MYLIP); lymphocyte transmembrane adaptor 1 (LAX1) and Early growth response 1 (ERG1) were age-repressed. Protocadherin 9 (PCDH9) was not amplified in HPC from elderly donors whereas interleukine 7 receptor (IL7R) was not amplified in young samples (not presented in the figure). Differential gene expression was always calculated in relation to the mean of young samples. The mean fold-ratio (±SD) is demonstrated for median aged and old donor samples. RT-PCR results (red) were always in line with microarray data (blue) for all genes tested.
    Figure Legend Snippet: QRT-PCR validation of differential gene expression. Age associated gene expression changes in MSC were validated by quantitative RT-PCR. 10 genes were selected that have been differentially expressed in microarray data (A): regeneration associated muscle protease (RAMP); maternally expressed 3 (MEG3); interleukin 13 receptor, alpha 2 (IL13RA2); S100 calcium binding protein A4 (S100A4) and triggering receptor expressed on myeloid cells 1 (TREM1) were age-induced. Homeobox B3 (HOXB3) and Homeobox B7 (HOXB7); midline 1 (MID1); small nuclear RNA activating complex, polypeptide 5 (SNAPC5) and peroxisome proliferator-activated receptor gamma (PPARG) were age-repressed. Furthermore, we have validated age associated changes in HPC for 9 genes (B): S100 calcium binding protein A10 (S100A10); vimentin (VIM); myeloid-associated differentiation marker (MYADM); pim-1 oncogene (PIM1) and annexin A2 (ANXA2) were age-induced. Timeless interacting protein (TIPIN); myosin regulatory light chain interacting protein (MYLIP); lymphocyte transmembrane adaptor 1 (LAX1) and Early growth response 1 (ERG1) were age-repressed. Protocadherin 9 (PCDH9) was not amplified in HPC from elderly donors whereas interleukine 7 receptor (IL7R) was not amplified in young samples (not presented in the figure). Differential gene expression was always calculated in relation to the mean of young samples. The mean fold-ratio (±SD) is demonstrated for median aged and old donor samples. RT-PCR results (red) were always in line with microarray data (blue) for all genes tested.

    Techniques Used: Quantitative RT-PCR, Expressing, Microarray, Binding Assay, Marker, Amplification, Reverse Transcription Polymerase Chain Reaction

    19) Product Images from "NOTCH SIGNALLING MODULATES HYPOXIA-INDUCED NEUROENDOCRINE DIFFERENTIATION OF HUMAN PROSTATE CANCER CELLS"

    Article Title: NOTCH SIGNALLING MODULATES HYPOXIA-INDUCED NEUROENDOCRINE DIFFERENTIATION OF HUMAN PROSTATE CANCER CELLS

    Journal: Molecular cancer research : MCR

    doi: 10.1158/1541-7786.MCR-11-0296

    A–C. LNCaP cells virally transduced with LacZ or with a dominant negative form of Hes1 (dnHes1), in frame with a HA-tag, were analyzed for Ngn3 and CGA mRNA expression 4 days after transduction, by quantitative real time PCR ( A–B ) and
    Figure Legend Snippet: A–C. LNCaP cells virally transduced with LacZ or with a dominant negative form of Hes1 (dnHes1), in frame with a HA-tag, were analyzed for Ngn3 and CGA mRNA expression 4 days after transduction, by quantitative real time PCR ( A–B ) and

    Techniques Used: Transduction, Dominant Negative Mutation, Expressing, Real-time Polymerase Chain Reaction

    20) Product Images from "Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae"

    Article Title: Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0174069

    Percentage of dead seedling by P . nicotianae (A) and quantification of P . nicotianae (B) for each treatment after harvesting. (P_CPTh): compost and black peat fortified with T . harzianum ; (P_CPTa): compost and black peat fortified with T . asperellum (P_CP): compost and black peat (P_P): Peat, inoculated with P . nicotianae .
    Figure Legend Snippet: Percentage of dead seedling by P . nicotianae (A) and quantification of P . nicotianae (B) for each treatment after harvesting. (P_CPTh): compost and black peat fortified with T . harzianum ; (P_CPTa): compost and black peat fortified with T . asperellum (P_CP): compost and black peat (P_P): Peat, inoculated with P . nicotianae .

    Techniques Used:

    Composition of fungal communities at phylum level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Composition of fungal communities at phylum level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Principal components analysis of fungal communities (genera) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Principal components analysis of fungal communities (genera) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Principal components analysis of bacterial communities (phylum) (A) and genera (B) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Principal components analysis of bacterial communities (phylum) (A) and genera (B) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Composition of Bacterial communities at phylum/class level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Composition of Bacterial communities at phylum/class level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    21) Product Images from "Changes in Maternal liver Cyp2c and Cyp2d Expression and Activity During Rat Pregnancy"

    Article Title: Changes in Maternal liver Cyp2c and Cyp2d Expression and Activity During Rat Pregnancy

    Journal: Biochemical pharmacology

    doi: 10.1016/j.bcp.2008.01.012

    Correlation between the fold difference in Cyp2d2 and nuclear receptor mRNA levels for non-pregnant, day 9, and day 19 pregnant rats. The fold difference in Cyp2d2 mRNA levels was plotted versus the fold difference in A) HNF3β, B) RARα,
    Figure Legend Snippet: Correlation between the fold difference in Cyp2d2 and nuclear receptor mRNA levels for non-pregnant, day 9, and day 19 pregnant rats. The fold difference in Cyp2d2 mRNA levels was plotted versus the fold difference in A) HNF3β, B) RARα,

    Techniques Used:

    22) Product Images from "LuxS-Based Signaling Affects Streptococcus mutans Biofilm Formation"

    Article Title: LuxS-Based Signaling Affects Streptococcus mutans Biofilm Formation

    Journal:

    doi: 10.1128/AEM.71.5.2372-2380.2005

    Real-time quantitative RT-PCR analysis of gtfB , gtfC , and gtfD gene expression at different growth stages of S. mutans GS-5 and the luxS mutant. The relative quantities of each gtf cDNA from S. mutans GS-5 and the luxS mutant were assessed by TaqMan assays.
    Figure Legend Snippet: Real-time quantitative RT-PCR analysis of gtfB , gtfC , and gtfD gene expression at different growth stages of S. mutans GS-5 and the luxS mutant. The relative quantities of each gtf cDNA from S. mutans GS-5 and the luxS mutant were assessed by TaqMan assays.

    Techniques Used: Quantitative RT-PCR, Expressing, Mutagenesis

    23) Product Images from "Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression"

    Article Title: Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.5161-13.2014

    Reduced Trpa1 mRNA levels in DRG sections of SNS-gp130 −/− mice. A , ISH with Trpa1 antisense riboprobe produced a selective staining of Trpa1 -expressing neurons, whereas sense probes did not show specific staining in the lumbar DRG sections. Scale bar, 50 μm. B , Samples of gp130 fl/fl and SNS-gp130 −/− lumbar DRG sections with Trpa1 antisense riboprobe display reduced Trpa1 mRNA labeling in SNS-gp130 −/− sections. Scale bar, 50 μm. C , Quantification of the ISH labeling in lumbar gp130 fl/fl and SNS-gp130 −/− sections. SNS-gp130 −/− sections reveal significantly lower numbers of Trpa1 expressing neurons. * p
    Figure Legend Snippet: Reduced Trpa1 mRNA levels in DRG sections of SNS-gp130 −/− mice. A , ISH with Trpa1 antisense riboprobe produced a selective staining of Trpa1 -expressing neurons, whereas sense probes did not show specific staining in the lumbar DRG sections. Scale bar, 50 μm. B , Samples of gp130 fl/fl and SNS-gp130 −/− lumbar DRG sections with Trpa1 antisense riboprobe display reduced Trpa1 mRNA labeling in SNS-gp130 −/− sections. Scale bar, 50 μm. C , Quantification of the ISH labeling in lumbar gp130 fl/fl and SNS-gp130 −/− sections. SNS-gp130 −/− sections reveal significantly lower numbers of Trpa1 expressing neurons. * p

    Techniques Used: Mouse Assay, In Situ Hybridization, Produced, Staining, Expressing, Labeling

    Trpa1 deficiency was predominantly observed in IB4-binding nociceptive neurons. A , B , Staining for IB4+ neurons in gp130 fl/fl and SNS-gp130 −/− DRG sections. Scale bar, 50 μm. C , The percentage of IB4-binding neurons was similar in both genotypes. D , E , FISH for Trpa1 mRNA in gp130 fl/fl and SNS-gp130 −/− DRG sections. Scale bar, 50 μm. F , Quantification of FISH shows that the number of Trpa1 mRNA-expressing neurons ( Trpa1 + ) was significantly reduced in SNS-gp130 −/− mice. G , H , Colabeling of Trpa1 mRNA expressing (magenta) and IB4-binding (green) neurons in both genotypes. Scale bar, 50 μm. I , In control mice, Trpa1 mRNA was predominantly expressed in IB4-positive neurons. In SNS-gp130 −/− mice, reduction of Trpa1 was most noticeable in the IB4-positive population. n.s., Not significant ( p > 0.05). * p
    Figure Legend Snippet: Trpa1 deficiency was predominantly observed in IB4-binding nociceptive neurons. A , B , Staining for IB4+ neurons in gp130 fl/fl and SNS-gp130 −/− DRG sections. Scale bar, 50 μm. C , The percentage of IB4-binding neurons was similar in both genotypes. D , E , FISH for Trpa1 mRNA in gp130 fl/fl and SNS-gp130 −/− DRG sections. Scale bar, 50 μm. F , Quantification of FISH shows that the number of Trpa1 mRNA-expressing neurons ( Trpa1 + ) was significantly reduced in SNS-gp130 −/− mice. G , H , Colabeling of Trpa1 mRNA expressing (magenta) and IB4-binding (green) neurons in both genotypes. Scale bar, 50 μm. I , In control mice, Trpa1 mRNA was predominantly expressed in IB4-positive neurons. In SNS-gp130 −/− mice, reduction of Trpa1 was most noticeable in the IB4-positive population. n.s., Not significant ( p > 0.05). * p

    Techniques Used: Binding Assay, Staining, Fluorescence In Situ Hybridization, Expressing, Mouse Assay

    Neuronal gp130 was essential for Trpa1 mRNA expression in DRG. A , TaqMan mRNA analysis revealed significantly lower Trpa1 mRNA levels in DRG explants and primary neuronal cultures of SNS-gp130 −/− mice (DRG from thoracic and lumbar levels). B , Trpa1 mRNA expression was significantly reduced in lumbar DRG explants from SNS-gp130 −/− mice compared with gp130 fl/fl and SNS-Cre , which were not significantly different. Furthermore, Trpv1 and Trpv4 mRNA levels were similar between gp130 fl/fl and SNS-gp130 −/− mice. C , Trpa1 mRNA expression of lumbar DRG explants within the first 14 postnatal days. In SNS-gp130 −/− mice, Trpa1 mRNA expression was already reduced at P1–P14. Expression levels are normalized to adult gp130 fl/fl mice. * p
    Figure Legend Snippet: Neuronal gp130 was essential for Trpa1 mRNA expression in DRG. A , TaqMan mRNA analysis revealed significantly lower Trpa1 mRNA levels in DRG explants and primary neuronal cultures of SNS-gp130 −/− mice (DRG from thoracic and lumbar levels). B , Trpa1 mRNA expression was significantly reduced in lumbar DRG explants from SNS-gp130 −/− mice compared with gp130 fl/fl and SNS-Cre , which were not significantly different. Furthermore, Trpv1 and Trpv4 mRNA levels were similar between gp130 fl/fl and SNS-gp130 −/− mice. C , Trpa1 mRNA expression of lumbar DRG explants within the first 14 postnatal days. In SNS-gp130 −/− mice, Trpa1 mRNA expression was already reduced at P1–P14. Expression levels are normalized to adult gp130 fl/fl mice. * p

    Techniques Used: Expressing, Mouse Assay

    24) Product Images from "Influence of surgical manipulation and surgical modality on the molecular detection of circulating tumor cells from colorectal cancer"

    Article Title: Influence of surgical manipulation and surgical modality on the molecular detection of circulating tumor cells from colorectal cancer

    Journal: Journal of the Korean Surgical Society

    doi: 10.4174/jkss.2012.82.6.356

    Comparison of circulating tumor markers (sampled from the inferior mesenteric vein) between the pre-mobilization and post-mobilization time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20. a) P
    Figure Legend Snippet: Comparison of circulating tumor markers (sampled from the inferior mesenteric vein) between the pre-mobilization and post-mobilization time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20. a) P

    Techniques Used:

    Comparison of circulating tumor markers (sampled from the peripheral vein) between the pre-operative and post-operative time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20. a) P
    Figure Legend Snippet: Comparison of circulating tumor markers (sampled from the peripheral vein) between the pre-operative and post-operative time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20. a) P

    Techniques Used:

    Comparison of circulating tumor markers (sampled from the peripheral vein) between the pre-operative and post-operative day 4 time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20.
    Figure Legend Snippet: Comparison of circulating tumor markers (sampled from the peripheral vein) between the pre-operative and post-operative day 4 time-points. CEA, carcinoembryonic antigen; CK20, cytokeratin-20.

    Techniques Used:

    25) Product Images from "Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions"

    Article Title: Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2019.e01246

    Expression of TNF-α in hepatopancreas and kidney of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene TNF-α was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P
    Figure Legend Snippet: Expression of TNF-α in hepatopancreas and kidney of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene TNF-α was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P

    Techniques Used: Expressing

    26) Product Images from "The Effects of Knockdown of Rho-Associated Kinase 1 and Zipper-Interacting Protein Kinase on Gene Expression and Function in Cultured Human Arterial Smooth Muscle Cells"

    Article Title: The Effects of Knockdown of Rho-Associated Kinase 1 and Zipper-Interacting Protein Kinase on Gene Expression and Function in Cultured Human Arterial Smooth Muscle Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0116969

    Effects of ROCK1 and ZIPK knockdown on cell proliferation. BrDU incorporation was observed by anti-BrDU staining in UASMC 48 h following transfection with siRNA to ZIPK or ROCK1 or with negative control siRNA. (A) Representative fields of cells are shown stained with anti-BrDU (top panels) or Hoechst nuclear stain (middle panels) with merged images shown in the bottom panels. (B) Quantitative data showing the numbers of BrDU-positive cells (left panel), total numbers of cells (middle panel) and % BrDU-positive cells (right panel). Fields were chosen to contain approximately the same number of cells (as shown in the middle panel) although, as shown by the MTT assay ( Fig. 5 ), ROCK1 knockdown reduced overall cell viability. Values represent means ± SEM ( n = 4 independent experiments, in each of which 3 fields of cells were counted for control, ZIPK knockdown and ROCK1 knockdown). *significantly different from control (p
    Figure Legend Snippet: Effects of ROCK1 and ZIPK knockdown on cell proliferation. BrDU incorporation was observed by anti-BrDU staining in UASMC 48 h following transfection with siRNA to ZIPK or ROCK1 or with negative control siRNA. (A) Representative fields of cells are shown stained with anti-BrDU (top panels) or Hoechst nuclear stain (middle panels) with merged images shown in the bottom panels. (B) Quantitative data showing the numbers of BrDU-positive cells (left panel), total numbers of cells (middle panel) and % BrDU-positive cells (right panel). Fields were chosen to contain approximately the same number of cells (as shown in the middle panel) although, as shown by the MTT assay ( Fig. 5 ), ROCK1 knockdown reduced overall cell viability. Values represent means ± SEM ( n = 4 independent experiments, in each of which 3 fields of cells were counted for control, ZIPK knockdown and ROCK1 knockdown). *significantly different from control (p

    Techniques Used: BrdU Incorporation Assay, BrdU Staining, Transfection, Negative Control, Staining, MTT Assay

    Effects of ROCK1 and ZIPK knockdown on IL-1β protein expression. IL-1β protein expression in control cells and cells transfected with ZIPK or ROCK1 siRNA was examined by western blotting. (A) Two representative western blots showing the levels of IL-1β in control UASMC and in UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. Two loading controls were used: α-actin and SM-22. (B) Quantification of IL-1β expression levels relative to α-actin (upper panel) and SM-22 (lower panel) in control, ZIPK- and ROCK1-knockdown UASMC. Values represent means ± SEM ( n = 13 for α-actin and n = 9 for SM-22). *significantly different from control ( p
    Figure Legend Snippet: Effects of ROCK1 and ZIPK knockdown on IL-1β protein expression. IL-1β protein expression in control cells and cells transfected with ZIPK or ROCK1 siRNA was examined by western blotting. (A) Two representative western blots showing the levels of IL-1β in control UASMC and in UASMC 48 h after transfection with siRNAs to ZIPK or ROCK1. Two loading controls were used: α-actin and SM-22. (B) Quantification of IL-1β expression levels relative to α-actin (upper panel) and SM-22 (lower panel) in control, ZIPK- and ROCK1-knockdown UASMC. Values represent means ± SEM ( n = 13 for α-actin and n = 9 for SM-22). *significantly different from control ( p

    Techniques Used: Expressing, Transfection, Western Blot

    Gene expression profiles. Heat maps to indicate the most differentially expressed genes in (A) ROCK1 knockdown ( > 2.4-fold change) and (B) ZIPK knockdown ( > 2-fold change) CASMC. Colored bands represent the change of the indicated gene expression: down-regulation green and up-regulation red. The key to decipher the color is shown below the clustering image. Results from the triplicate analyses are included.
    Figure Legend Snippet: Gene expression profiles. Heat maps to indicate the most differentially expressed genes in (A) ROCK1 knockdown ( > 2.4-fold change) and (B) ZIPK knockdown ( > 2-fold change) CASMC. Colored bands represent the change of the indicated gene expression: down-regulation green and up-regulation red. The key to decipher the color is shown below the clustering image. Results from the triplicate analyses are included.

    Techniques Used: Expressing

    Knockdown of ROCK1 and ZIPK at the protein level in CASMC. CASMC were transfected with siRNAs to ROCK1 or ZIPK or with negative control siRNAs. Cells were lysed 48 h later for western blotting with anti-ROCK1 and anti-ZIPK. Loading levels were normalized using anti-GAPDH. Representative results are shown for two of a total of 10 independent experiments. Quantitative data are presented in Table 2 .
    Figure Legend Snippet: Knockdown of ROCK1 and ZIPK at the protein level in CASMC. CASMC were transfected with siRNAs to ROCK1 or ZIPK or with negative control siRNAs. Cells were lysed 48 h later for western blotting with anti-ROCK1 and anti-ZIPK. Loading levels were normalized using anti-GAPDH. Representative results are shown for two of a total of 10 independent experiments. Quantitative data are presented in Table 2 .

    Techniques Used: Transfection, Negative Control, Western Blot

    Effects of ROCK1 and ZIPK knockdown on vascular smooth muscle cell viability. The MTT cell viability assay was performed on CASMC and UASMC 48 h after transfection with siRNA to ROCK1 or ZIPK or with negative control siRNA. Values represent the means ± SEM ( n = 8). *significantly different from control ( p
    Figure Legend Snippet: Effects of ROCK1 and ZIPK knockdown on vascular smooth muscle cell viability. The MTT cell viability assay was performed on CASMC and UASMC 48 h after transfection with siRNA to ROCK1 or ZIPK or with negative control siRNA. Values represent the means ± SEM ( n = 8). *significantly different from control ( p

    Techniques Used: MTT Assay, Viability Assay, Transfection, Negative Control

    Effects of ROCK1 and ZIPK knockdown on IL-6 and IL-1β mRNA expression and IL-6 protein secretion. (A) CASMCs were transfected with siRNA to ZIPK or ROCK1 or with negative control siRNA. Cells were lysed 48 h later for qRT-PCR to quantify IL-6 and IL-1β mRNA levels. Values represent means ± SEM ( n = 7 for IL-6 and n = 6 for IL-1β). *significantly different from control (p
    Figure Legend Snippet: Effects of ROCK1 and ZIPK knockdown on IL-6 and IL-1β mRNA expression and IL-6 protein secretion. (A) CASMCs were transfected with siRNA to ZIPK or ROCK1 or with negative control siRNA. Cells were lysed 48 h later for qRT-PCR to quantify IL-6 and IL-1β mRNA levels. Values represent means ± SEM ( n = 7 for IL-6 and n = 6 for IL-1β). *significantly different from control (p

    Techniques Used: Expressing, Transfection, Negative Control, Quantitative RT-PCR

    27) Product Images from "Endogenous Tumor Necrosis Factor α (TNFα) Requires TNF Receptor Type 2 to Generate Heat Hyperalgesia in a Mouse Cancer Model"

    Article Title: Endogenous Tumor Necrosis Factor α (TNFα) Requires TNF Receptor Type 2 to Generate Heat Hyperalgesia in a Mouse Cancer Model

    Journal: The Journal of Neuroscience

    doi: 10.1523/JNEUROSCI.4476-07.2008

    Heat hyperalgesia in the mice with tumor depended on TRPV1. A , Western blot analysis of TRPV1 expression in L3, L4, and L5 DRG neurons from control and tumor mice. TRPV1 protein level is 2.1-fold increased in tumor compared with naive mice. B , TRPV1 levels are significantly increased in tumor-bearing wild-type ( n = 6) but not TNFR2 −/− ( n = 3) mice. C , Immunocytochemistry illustrates an increase in TRPV1 levels after TNFα (10 ng/ml) stimulation of DRG neurons in culture. Scale bar, 50 μm. The intensity of TRPV1 labeling increased significantly 1 and 2 h after TNFα (10 ng/ml) stimulation ( p
    Figure Legend Snippet: Heat hyperalgesia in the mice with tumor depended on TRPV1. A , Western blot analysis of TRPV1 expression in L3, L4, and L5 DRG neurons from control and tumor mice. TRPV1 protein level is 2.1-fold increased in tumor compared with naive mice. B , TRPV1 levels are significantly increased in tumor-bearing wild-type ( n = 6) but not TNFR2 −/− ( n = 3) mice. C , Immunocytochemistry illustrates an increase in TRPV1 levels after TNFα (10 ng/ml) stimulation of DRG neurons in culture. Scale bar, 50 μm. The intensity of TRPV1 labeling increased significantly 1 and 2 h after TNFα (10 ng/ml) stimulation ( p

    Techniques Used: Mouse Assay, Western Blot, Expressing, Immunocytochemistry, Labeling

    28) Product Images from "Impact of Exogenous Galectin-9 on Human T Cells"

    Article Title: Impact of Exogenous Galectin-9 on Human T Cells

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M115.661272

    Gal-9 induces IL-2 and IFNγ production in Jurkat and peripheral T cells. A, quantitative real time PCR determination of IL-2 mRNA expression in Jurkat, JCaM1.6 ( upper histogram ), or J31.13 ( lower histogram ) cells treated with either gal-9 (30
    Figure Legend Snippet: Gal-9 induces IL-2 and IFNγ production in Jurkat and peripheral T cells. A, quantitative real time PCR determination of IL-2 mRNA expression in Jurkat, JCaM1.6 ( upper histogram ), or J31.13 ( lower histogram ) cells treated with either gal-9 (30

    Techniques Used: Real-time Polymerase Chain Reaction, Expressing

    29) Product Images from "Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1"

    Article Title: Functional Characterization of New Polyketide Synthase Genes Involved in Ochratoxin A Biosynthesis in Aspergillus Ochraceus fc-1

    Journal: Toxins

    doi: 10.3390/toxins7082723

    ( A ) The changes in the amount of OTA product at different time points during the growth of A. ochraceus fc-1. OTA concentrations were determined by HPLC-FLD using an OTA standard; ( B ) Relative expression of the AoOTApks-1 and -2 genes was assayed at different time points using a 7500 real-time PCR system. The glyceraldehyde 3-phosphate dehydrogenase (GADPH) gene was used as a control.
    Figure Legend Snippet: ( A ) The changes in the amount of OTA product at different time points during the growth of A. ochraceus fc-1. OTA concentrations were determined by HPLC-FLD using an OTA standard; ( B ) Relative expression of the AoOTApks-1 and -2 genes was assayed at different time points using a 7500 real-time PCR system. The glyceraldehyde 3-phosphate dehydrogenase (GADPH) gene was used as a control.

    Techniques Used: High Performance Liquid Chromatography, Expressing, Real-time Polymerase Chain Reaction

    30) Product Images from "RPAD (RNase R Treatment, Polyadenylation, and Poly(A)+ RNA Depletion) Method to Isolate Highly Pure Circular RNA"

    Article Title: RPAD (RNase R Treatment, Polyadenylation, and Poly(A)+ RNA Depletion) Method to Isolate Highly Pure Circular RNA

    Journal: Methods (San Diego, Calif.)

    doi: 10.1016/j.ymeth.2018.10.022

    Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.
    Figure Legend Snippet: Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.

    Techniques Used: Quantitative RT-PCR

    31) Product Images from "Nicotinamide Exacerbates Hypoxemia in Ventilator-Induced Lung Injury Independent of Neutrophil Infiltration"

    Article Title: Nicotinamide Exacerbates Hypoxemia in Ventilator-Induced Lung Injury Independent of Neutrophil Infiltration

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0123460

    Nicotinamide and mechanical ventilation decrease lung expression of Cebpe . Mice were anesthetized and placed on mechanical ventilation as described in Fig 1 and received either PBS (PBS+MV) or nicotinamide at 400 mg/kg (NAM+MV) after 1 hour of mechanical ventilation. Control mice were allowed to continue spontaneously breathing and received PBS or nicotinamide at the same time as mice on mechanical ventilation (PBS, NAM). Mice were euthanized at the end of 6 hours mechanical ventilation, and the left lung was placed in RNeasy buffer and homogenized. RT-PCR was performed using TaqMan primers for (A) Nampt ; (B) Sirt1 ; (C) Cebpa ; (D) Cebpb ; and (E) Cebpe Significance is indicated for comparisons between PBS and PBS+MV, and between PBS+MV and NAM+MV unless otherwise shown. * p
    Figure Legend Snippet: Nicotinamide and mechanical ventilation decrease lung expression of Cebpe . Mice were anesthetized and placed on mechanical ventilation as described in Fig 1 and received either PBS (PBS+MV) or nicotinamide at 400 mg/kg (NAM+MV) after 1 hour of mechanical ventilation. Control mice were allowed to continue spontaneously breathing and received PBS or nicotinamide at the same time as mice on mechanical ventilation (PBS, NAM). Mice were euthanized at the end of 6 hours mechanical ventilation, and the left lung was placed in RNeasy buffer and homogenized. RT-PCR was performed using TaqMan primers for (A) Nampt ; (B) Sirt1 ; (C) Cebpa ; (D) Cebpb ; and (E) Cebpe Significance is indicated for comparisons between PBS and PBS+MV, and between PBS+MV and NAM+MV unless otherwise shown. * p

    Techniques Used: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction

    32) Product Images from "Uncoupling protein 2 regulates daily rhythms of insulin secretion capacity in MIN6 cells and isolated islets from male mice"

    Article Title: Uncoupling protein 2 regulates daily rhythms of insulin secretion capacity in MIN6 cells and isolated islets from male mice

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2017.04.008

    Dynamic expression pattern of Ucp2 regulates the temporal capacity of GSIS in MIN6 cells. (A) Ucp2 mRNA expression levels at 4 h intervals over 36 h in synchronized MIN6 cells. Ucp2 mRNA shown is expressed relative to Eif 2α levels and normalized to mRNA levels at time 0 (immediately after synchronization). N = 4–6 independent experiments. (B) i. GSIS capacity of synchronized MIN6 cells every 4 h over 36 h as measured by static incubation assay. Open circles represent insulin secreted when MIN6 cells are exposed to low (2.8 mM) glucose. Closed circles represent insulin secretion when MIN6 cells are exposed to high glucose (16.7 mM). N = 7–8 independent experiments. ii. Average GSIS capacity over segmented 12 h periods. GSIS is presented as the fold-change in insulin secretion above basal (2.8 mM glucose) when stimulated with high (16.7 mM) glucose. (C) GSIS capacity at 4 and 16 h post-synchronization in MIN6 cells treated with (hatched bars) and without genipin (solid black bars), a UCP2 activity inhibitor. Genipin was applied at a final concentration of 50 μM, 1 h before start of GSIS assay and remained present throughout the assay. N = 5. *p
    Figure Legend Snippet: Dynamic expression pattern of Ucp2 regulates the temporal capacity of GSIS in MIN6 cells. (A) Ucp2 mRNA expression levels at 4 h intervals over 36 h in synchronized MIN6 cells. Ucp2 mRNA shown is expressed relative to Eif 2α levels and normalized to mRNA levels at time 0 (immediately after synchronization). N = 4–6 independent experiments. (B) i. GSIS capacity of synchronized MIN6 cells every 4 h over 36 h as measured by static incubation assay. Open circles represent insulin secreted when MIN6 cells are exposed to low (2.8 mM) glucose. Closed circles represent insulin secretion when MIN6 cells are exposed to high glucose (16.7 mM). N = 7–8 independent experiments. ii. Average GSIS capacity over segmented 12 h periods. GSIS is presented as the fold-change in insulin secretion above basal (2.8 mM glucose) when stimulated with high (16.7 mM) glucose. (C) GSIS capacity at 4 and 16 h post-synchronization in MIN6 cells treated with (hatched bars) and without genipin (solid black bars), a UCP2 activity inhibitor. Genipin was applied at a final concentration of 50 μM, 1 h before start of GSIS assay and remained present throughout the assay. N = 5. *p

    Techniques Used: Expressing, Incubation, Activity Assay, Concentration Assay

    33) Product Images from "Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene"

    Article Title: Human malaria diagnosis using a single-step direct-PCR based on the Plasmodium cytochrome oxidase III gene

    Journal: Malaria Journal

    doi: 10.1186/s12936-016-1185-x

    Lowest parasitaemia detected for the COX-III single direct-PCR and the 18s-rRNA nested-PCR on positive controls. a The lowest for P. vivax was 2 parasites/μL using the COX-III based PCR (expected fragment around 500 bp). b For P. falciparum (with COX-III PCR) was 0.6 parasites/μL. Adding one blood spot from healthy donors A and B to the parasite dilutions did not affect the PCR performance. Donor’s blood did not amplify any bands. c Performance of the 18s-rRNA genus-malaria nested PCR on nine positive control samples collected in the field (band of 235 bp). Non-specific bands are present. d Plasmodium vivax (121 bp), P. falciparum (200 bp) and a mixed infection detected in positive controls using the species-specific nested PCR
    Figure Legend Snippet: Lowest parasitaemia detected for the COX-III single direct-PCR and the 18s-rRNA nested-PCR on positive controls. a The lowest for P. vivax was 2 parasites/μL using the COX-III based PCR (expected fragment around 500 bp). b For P. falciparum (with COX-III PCR) was 0.6 parasites/μL. Adding one blood spot from healthy donors A and B to the parasite dilutions did not affect the PCR performance. Donor’s blood did not amplify any bands. c Performance of the 18s-rRNA genus-malaria nested PCR on nine positive control samples collected in the field (band of 235 bp). Non-specific bands are present. d Plasmodium vivax (121 bp), P. falciparum (200 bp) and a mixed infection detected in positive controls using the species-specific nested PCR

    Techniques Used: Polymerase Chain Reaction, Nested PCR, Positive Control, Infection

    Performance of the 18s-rRNA nested-PCR and COX-III single direct-PCR on samples from the Solomon Islands. a Strong non-specific bands in 14 samples were amplified when the genus-malaria 18s-rRNA nested PCR was used. The expected diagnostic band for Plasmodium spp is 235 bp ( red arrow ). b Nonspecific bands close to the 121 bp diagnostic bands for P. vivax ( blue arrow ); only the sample 13 shown a robust band. c Eighteen samples tested for Plasmodium spp using the COX-III gene, a band of approximately 500 bp ( green arrow ) represent positive samples
    Figure Legend Snippet: Performance of the 18s-rRNA nested-PCR and COX-III single direct-PCR on samples from the Solomon Islands. a Strong non-specific bands in 14 samples were amplified when the genus-malaria 18s-rRNA nested PCR was used. The expected diagnostic band for Plasmodium spp is 235 bp ( red arrow ). b Nonspecific bands close to the 121 bp diagnostic bands for P. vivax ( blue arrow ); only the sample 13 shown a robust band. c Eighteen samples tested for Plasmodium spp using the COX-III gene, a band of approximately 500 bp ( green arrow ) represent positive samples

    Techniques Used: Nested PCR, Polymerase Chain Reaction, Amplification, Diagnostic Assay

    Flowchart of PCR techniques and alignment of the Plasmodium cytochrome oxidase gene III PCR products. For the cytochrome oxidase III (COX-III) gene the expected sizes of PCR fragments are: P. vivax (506 bp), P. falciparum (508 bp), P. malariae (504 bp), P. knowlesi (499 bp), P. ovale wallikeri and P. ovale curtisi (506 bp). With the COX-III gene sequence alignment, 140 polymorphisms (28 % of nucleotides) provide information for species diagnosis (there are eight blocks of highly polymorphic regions). For differentiation between P. ovale wallikeri and P. ovale curtisi, 16 SNPs were found ( green arrows ). When using 18s-rRNA nested PCR, additional Nest2 PCRs with species-specific primers are required for diagnosis of P. malariae, P. ovale wallikeri, P. ovale curtisi and P. knowlesi
    Figure Legend Snippet: Flowchart of PCR techniques and alignment of the Plasmodium cytochrome oxidase gene III PCR products. For the cytochrome oxidase III (COX-III) gene the expected sizes of PCR fragments are: P. vivax (506 bp), P. falciparum (508 bp), P. malariae (504 bp), P. knowlesi (499 bp), P. ovale wallikeri and P. ovale curtisi (506 bp). With the COX-III gene sequence alignment, 140 polymorphisms (28 % of nucleotides) provide information for species diagnosis (there are eight blocks of highly polymorphic regions). For differentiation between P. ovale wallikeri and P. ovale curtisi, 16 SNPs were found ( green arrows ). When using 18s-rRNA nested PCR, additional Nest2 PCRs with species-specific primers are required for diagnosis of P. malariae, P. ovale wallikeri, P. ovale curtisi and P. knowlesi

    Techniques Used: Polymerase Chain Reaction, Sequencing, Nested PCR

    34) Product Images from "Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae"

    Article Title: Relationship of microbial communities and suppressiveness of Trichoderma fortified composts for pepper seedlings infected by Phytophthora nicotianae

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0174069

    Percentage of dead seedling by P . nicotianae (A) and quantification of P . nicotianae (B) for each treatment after harvesting. (P_CPTh): compost and black peat fortified with T . harzianum ; (P_CPTa): compost and black peat fortified with T . asperellum (P_CP): compost and black peat (P_P): Peat, inoculated with P . nicotianae .
    Figure Legend Snippet: Percentage of dead seedling by P . nicotianae (A) and quantification of P . nicotianae (B) for each treatment after harvesting. (P_CPTh): compost and black peat fortified with T . harzianum ; (P_CPTa): compost and black peat fortified with T . asperellum (P_CP): compost and black peat (P_P): Peat, inoculated with P . nicotianae .

    Techniques Used:

    Composition of fungal communities at phylum level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Composition of fungal communities at phylum level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Principal components analysis of fungal communities (genera) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Principal components analysis of fungal communities (genera) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Principal components analysis of bacterial communities (phylum) (A) and genera (B) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Principal components analysis of bacterial communities (phylum) (A) and genera (B) of all treatments after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    Composition of Bacterial communities at phylum/class level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .
    Figure Legend Snippet: Composition of Bacterial communities at phylum/class level of each treatment after harvesting. (CPTh): compost and black peat fortified with T . harzianum ; (CPTa): compost and black peat fortified with T . asperellum (CP): compost and black peat (P): peat. Treatments with the letter P_ ahead were the same but inoculated with P . nicotianae .

    Techniques Used:

    35) Product Images from "Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease"

    Article Title: Erythrosin B is a potent and broad-spectrum orthosteric inhibitor of the flavivirus NS2B-NS3 protease

    Journal: Antiviral research

    doi: 10.1016/j.antiviral.2017.12.018

    Inhibition of the NS2B-NS3 interactions and protease activity (A) Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66 to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.
    Figure Legend Snippet: Inhibition of the NS2B-NS3 interactions and protease activity (A) Dose-dependent inhibition of SLC upon binding of NLuc-NS2B 49-66 to GST-CLuc-NS3 by erythrosin B. N = 3. (B) Dose-response inhibitions of the DENV2 His-NS2B/His-MBP-NS3 protease activity by erythrosin B. N = 3. ( C ) Dose-response inhibitions of the ZIKV His-NS2B/GST-NS3 protease activity by erythrosin B. N = 3.

    Techniques Used: Inhibition, Activity Assay, Binding Assay

    Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.
    Figure Legend Snippet: Inhibition of NS2B-NS3 protease complex via a non-competitive mechanism ( A,B ) Inhibition kinetic experiment of the DENV2 His-NS2B/His-MBP-NS3 ( A ) and the ZIKV His-NS2B/GST-NS3 ( B ) heterocomplexes by erythrosin B. Lineweaver–Burk plots of kinetics experimental data for inhibition of the His-NS2B/His-MBP-NS3 hetero protease complexes by erythrosin B. The DENV2 MBP-NS3 or ZIKV GST-NS3 (100 nM) was mixed with erythrosin B (3 μM or various concentrations) for 30 min. The DENV2 His-NS2B or ZIKV His-NS2B (1 μM) were added together with the Abz substrate at various concentrations (800 μM–25 μM in 2-fold dilutions). ( C ) Superimposition of bound EB (yellow) with the NS2B co-factor (cyan) of DENV3 (PDB: 3U1I). NS3 residues in contact with EB were in stick representation (grey). ( D ) Ribbon presentation of erythrosin B (green) docked into NS3pro of DENV3 (PDB: 3U1I). Key interaction residues are highlighted in stick presentation. Hydrogen bonds are shown in yellow dotted lines and π - π stacking is shown in blue line. ( E ) Ribbon presentation of erythrosin B (green) docked into NS3pro of ZIKV (PDB: 5LC0). The corresponding NS3pro β -strand hairpin loops on ZIKV are shown in green and grey. Key interaction residues are highlighted in stick presentation. Hydrogen bonds are also shown in yellow dotted lines and π - π stacking is shown in blue lines.

    Techniques Used: Inhibition

    36) Product Images from "Nicotinamide Exacerbates Hypoxemia in Ventilator-Induced Lung Injury Independent of Neutrophil Infiltration"

    Article Title: Nicotinamide Exacerbates Hypoxemia in Ventilator-Induced Lung Injury Independent of Neutrophil Infiltration

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0123460

    Nicotinamide and mechanical ventilation decrease lung expression of Cebpe . Mice were anesthetized and placed on mechanical ventilation as described in Fig 1 and received either PBS (PBS+MV) or nicotinamide at 400 mg/kg (NAM+MV) after 1 hour of mechanical ventilation. Control mice were allowed to continue spontaneously breathing and received PBS or nicotinamide at the same time as mice on mechanical ventilation (PBS, NAM). Mice were euthanized at the end of 6 hours mechanical ventilation, and the left lung was placed in RNeasy buffer and homogenized. RT-PCR was performed using TaqMan primers for (A) Nampt ; (B) Sirt1 ; (C) Cebpa ; (D) Cebpb ; and (E) Cebpe Significance is indicated for comparisons between PBS and PBS+MV, and between PBS+MV and NAM+MV unless otherwise shown. * p
    Figure Legend Snippet: Nicotinamide and mechanical ventilation decrease lung expression of Cebpe . Mice were anesthetized and placed on mechanical ventilation as described in Fig 1 and received either PBS (PBS+MV) or nicotinamide at 400 mg/kg (NAM+MV) after 1 hour of mechanical ventilation. Control mice were allowed to continue spontaneously breathing and received PBS or nicotinamide at the same time as mice on mechanical ventilation (PBS, NAM). Mice were euthanized at the end of 6 hours mechanical ventilation, and the left lung was placed in RNeasy buffer and homogenized. RT-PCR was performed using TaqMan primers for (A) Nampt ; (B) Sirt1 ; (C) Cebpa ; (D) Cebpb ; and (E) Cebpe Significance is indicated for comparisons between PBS and PBS+MV, and between PBS+MV and NAM+MV unless otherwise shown. * p

    Techniques Used: Expressing, Mouse Assay, Reverse Transcription Polymerase Chain Reaction

    C/EBPε-deficient mice have decreased PMNs but develop equivalent hypoxemia and edema with VILI. (A) Wild-type (WT+MV, open circles) and Cebpe knockout ( Cebpe +MV, closed diamonds) mice were anesthetized and placed on mechanical ventilation (MV) with tidal volumes of 20 ml/kg and zero PEEP for a total of 6 hours. Oxygen saturation was measured each hour for six hours. n = 5/group. (B) Wild type and C/EBPε-deficient mice were anesthetized and placed on MV as described in (A) (WT+MV, +MV); control mice were not anesthetized or mechanically ventilated (WT, KO). All mice were euthanized at the end of 6 hours mechanical ventilation or spontaneous breathing (control mice). Bronchoalveolar lavage (BAL) fluid was assayed for: (B) protein concentration; and (C) neutrophils (PMNs). n = 4 /group (non-ventilated controls) and 5/group (mechanical ventilation). Significance is indicated for comparisons between WT+MV and Cebpe KO+MV groups in all graphs. * p
    Figure Legend Snippet: C/EBPε-deficient mice have decreased PMNs but develop equivalent hypoxemia and edema with VILI. (A) Wild-type (WT+MV, open circles) and Cebpe knockout ( Cebpe +MV, closed diamonds) mice were anesthetized and placed on mechanical ventilation (MV) with tidal volumes of 20 ml/kg and zero PEEP for a total of 6 hours. Oxygen saturation was measured each hour for six hours. n = 5/group. (B) Wild type and C/EBPε-deficient mice were anesthetized and placed on MV as described in (A) (WT+MV, +MV); control mice were not anesthetized or mechanically ventilated (WT, KO). All mice were euthanized at the end of 6 hours mechanical ventilation or spontaneous breathing (control mice). Bronchoalveolar lavage (BAL) fluid was assayed for: (B) protein concentration; and (C) neutrophils (PMNs). n = 4 /group (non-ventilated controls) and 5/group (mechanical ventilation). Significance is indicated for comparisons between WT+MV and Cebpe KO+MV groups in all graphs. * p

    Techniques Used: Mouse Assay, Knock-Out, Protein Concentration

    37) Product Images from "RPAD (RNase R Treatment, Polyadenylation, and Poly(A)+ RNA Depletion) Method to Isolate Highly Pure Circular RNA"

    Article Title: RPAD (RNase R Treatment, Polyadenylation, and Poly(A)+ RNA Depletion) Method to Isolate Highly Pure Circular RNA

    Journal: Methods (San Diego, Calif.)

    doi: 10.1016/j.ymeth.2018.10.022

    Schematic of the RPAD method. , RNAs isolated from above steps are used in RT-qPCR for validation of linear RNA depletion.
    Figure Legend Snippet: Schematic of the RPAD method. , RNAs isolated from above steps are used in RT-qPCR for validation of linear RNA depletion.

    Techniques Used: Isolation, Quantitative RT-PCR

    Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.
    Figure Legend Snippet: Depletion of linear RNA by RNase R digestion. RT-qPCR analysis of subsets of linear rRNA, mRNAs and circRNAs (A), as well as snRNAs and 5S rRNA (B) in HeLa cell RNA samples left untreated or treated with RNase R. In (A) since the circRNA levels were not affected by RNase R treatment, mRNAs left after RNase R treatment were normalized to the levels of the corresponding circRNAs. Data represent the means ± SEM from 4 or more experiments.

    Techniques Used: Quantitative RT-PCR

    38) Product Images from "Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer"

    Article Title: Growth inhibitory and chemo-sensitization effects of naringenin, a natural flavanone purified from Thymus vulgaris, on human breast and colorectal cancer

    Journal: Cancer Cell International

    doi: 10.1186/s12935-015-0194-0

    Nar enhances the chemosensitivity of human colorectal cancer cells to DNA-acting drugs. Human colorectal cancer cells SW1116 were plated (27 × 10 3 cells/well) into a 96-well plate at 37°C in a non-CO 2 incubator. At 18 h after starting the culture, the cells were treated for 24 h with Nar (1 mM) and various concentrations of camptothecin, CPT doxorubicin, DOX, 5-fluorouracil, 5FU cisplatin, CIP, etoposide, ETP ellipticine, ELP (1 × 10 −10 – 1 × 10 −3 M), carboplatin, CAP (1 × 10 −10 – 3.5 × 10 −4 M) and cyclophosphamide, CPA (1 × 10 −11 – 1 × 10 −5 M). Cell proliferation was monitored using an MTT assay.
    Figure Legend Snippet: Nar enhances the chemosensitivity of human colorectal cancer cells to DNA-acting drugs. Human colorectal cancer cells SW1116 were plated (27 × 10 3 cells/well) into a 96-well plate at 37°C in a non-CO 2 incubator. At 18 h after starting the culture, the cells were treated for 24 h with Nar (1 mM) and various concentrations of camptothecin, CPT doxorubicin, DOX, 5-fluorouracil, 5FU cisplatin, CIP, etoposide, ETP ellipticine, ELP (1 × 10 −10 – 1 × 10 −3 M), carboplatin, CAP (1 × 10 −10 – 3.5 × 10 −4 M) and cyclophosphamide, CPA (1 × 10 −11 – 1 × 10 −5 M). Cell proliferation was monitored using an MTT assay.

    Techniques Used: Cycling Probe Technology, MTT Assay

    Nar enhances the chemosensitivity of human breast cancer cells to DNA-acting drugs. Human breast cancer cells HTB26 were plated (27 × 10 3 cells/well) into a 96-well plate at 37°C in a non-CO 2 incubator. At 18 h after starting the culture, the cells were treated for 24 h with Nar (1 mM) and various concentrations of camptothecin, CPT, doxorubicin, DOX, 5-fluorouracil, 5FU, cisplatin, CIP, etoposide, ETP, ellipticine, ELP (1 × 10 −10 – 1 × 10 −3 M), carboplatin, CAP (1 × 10 −10 – 3.5 × 10 −4 M) and cyclophosphamide, CPA (1 × 10 −11 – 1 × 10 −5 M). Cell proliferation was monitored using an MTT assay.
    Figure Legend Snippet: Nar enhances the chemosensitivity of human breast cancer cells to DNA-acting drugs. Human breast cancer cells HTB26 were plated (27 × 10 3 cells/well) into a 96-well plate at 37°C in a non-CO 2 incubator. At 18 h after starting the culture, the cells were treated for 24 h with Nar (1 mM) and various concentrations of camptothecin, CPT, doxorubicin, DOX, 5-fluorouracil, 5FU, cisplatin, CIP, etoposide, ETP, ellipticine, ELP (1 × 10 −10 – 1 × 10 −3 M), carboplatin, CAP (1 × 10 −10 – 3.5 × 10 −4 M) and cyclophosphamide, CPA (1 × 10 −11 – 1 × 10 −5 M). Cell proliferation was monitored using an MTT assay.

    Techniques Used: Cycling Probe Technology, MTT Assay

    Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.
    Figure Legend Snippet: Time and dose-dependent anti-proliferative effects and inhibition of colony formation of Nar on human colorectal and breast cancer cell lines. Human colorectal cancer cells (SW1116, SW837) (A a- e) , human breast cancer cells (HTB 26, HTB132) (B a- e) and normal human fibroblast cells (CRL1554) (A, B a- e) were plated (27 × 10 3 cells/well) in 96-well plates in CO 2 and non-CO 2 incubators, depending on type of media and cells, at 37°C for 18 h. The cells were then treated with various concentrations of Nar (0.05 – 4.0 mM) for 3–24 h. Cell growth was monitored using an MTT assay. Untreated and Nar-treated colorectal cancer cells SW1116 (Ca) , SW837 (Cb) and Nar-treated breast cancer cells HTB26 (Cc) , HTB132 (Cd) were trypsinized, counted and plated (500 cells /well) in 6-well plates. Following 10–14 days of incubation in non-CO 2 incubator at 37°C, the colonies were fixed and stained with crystal violet. The stained colonies were counted and compared with the untreated control.

    Techniques Used: Inhibition, MTT Assay, Incubation, Staining

    39) Product Images from "Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions"

    Article Title: Validation of growth enhancing, immunostimulatory and disease resistance properties of Achyranthes aspera in Labeo rohita fry in pond conditions

    Journal: Heliyon

    doi: 10.1016/j.heliyon.2019.e01246

    Expression of lysozyme C and lysozyme G in hepatopancreas of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene lysozyme C/G was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P
    Figure Legend Snippet: Expression of lysozyme C and lysozyme G in hepatopancreas of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene lysozyme C/G was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P

    Techniques Used: Expressing

    Expression of lysozyme C and lysozyme G in kidney of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene lysozyme C/G was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P
    Figure Legend Snippet: Expression of lysozyme C and lysozyme G in kidney of L. rohita fed with three different diets and challenged with A. hydrophila . The relative expression of the target gene lysozyme C/G was normalized to the expression of β-actin (internal control) and expressed as fold changes relative to the control diet fed group. Vertical bars with different superscripts are significantly ( P

    Techniques Used: Expressing

    40) Product Images from "Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome"

    Article Title: Differences in the Osteogenic Differentiation Capacity of Omental Adipose-Derived Stem Cells in Obese Patients With and Without Metabolic Syndrome

    Journal: Endocrinology

    doi: 10.1210/en.2015-1413

    Expression of fibrotic proteins in the CD34 negative -enriched omental ASC fraction. A, Correlation between mRNA expression levels of NOX5 and Col1a1 (R = 0.555; P = .077), Col3a1 (R = 0.445; P = .170), Col5a1 (R = 0.627; P = .039), and Col6a3 (R = 0.645; P = .032). B, Correlation between HOMA-IR and Col1a1 (R = 0.600; P = .051), Col3a1 (R = 0.773; P = .005), Col5a1 (R = 0.445; P = .170), and Col6a3 (R = 0.045; P = .894). C, mRNA expression levels of Col1a1, Col3a1, Col5a1, and Col6a3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Col1a1, Col3a1, Col5a1, and Col6a3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P
    Figure Legend Snippet: Expression of fibrotic proteins in the CD34 negative -enriched omental ASC fraction. A, Correlation between mRNA expression levels of NOX5 and Col1a1 (R = 0.555; P = .077), Col3a1 (R = 0.445; P = .170), Col5a1 (R = 0.627; P = .039), and Col6a3 (R = 0.645; P = .032). B, Correlation between HOMA-IR and Col1a1 (R = 0.600; P = .051), Col3a1 (R = 0.773; P = .005), Col5a1 (R = 0.445; P = .170), and Col6a3 (R = 0.045; P = .894). C, mRNA expression levels of Col1a1, Col3a1, Col5a1, and Col6a3 in the CD34 negative -enriched omental ASC fraction of subjects grouped by metabolic profile. Col1a1, Col3a1, Col5a1, and Col6a3 mRNA values were quantified by RT-qPCR and normalized to mRNA RPL13A. Values are reported as mean ± SE (Nw, n = 3; Non-MS, n = 4; MS, n = 4). *, Results significantly different (Mann-Whitney; P

    Techniques Used: Expressing, Quantitative RT-PCR, Mass Spectrometry, MANN-WHITNEY

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