mgso4  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies mgso4
    Mgso4, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgso4/product/Integrated DNA Technologies
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2020-01
    93/100 stars

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    Related Articles

    DNA Extraction:

    Article Title:
    Article Snippet: To analyze RIPs of leading strand synthesis, 2 μM emetine (Sigma) was added to the cell culture media for 1 h before DNA isolation. .. One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology).

    Negative Control:

    Article Title:
    Article Snippet: One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology). .. As a negative control, a sample without template DNA was included in every experiment.

    Amplification:

    Article Title:
    Article Snippet: First round PCR Amplification of mtDNA was performed using a two step nested PCR protocol to avoid amplification of homologous regions in the nuclear DNA. .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl.

    Article Title:
    Article Snippet: Five sets of mitochondrial specific primer pairs were used, resulting in amplification product between 714 and 928 base pair in length (see Table ). .. The PCR reaction mixture contained 0.1 μl of extracted DNA (~5 ng), 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl.

    Article Title:
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Agarose Gel Electrophoresis:

    Article Title:
    Article Snippet: PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. Samples were loaded onto a 1.5% agarose gel and run for 25 min at 120 V. Subsequent treatment with ethidium bromide allowed visualization of the PCR product and gel purification.

    Cell Culture:

    Article Title:
    Article Snippet: To analyze RIPs of leading strand synthesis, 2 μM emetine (Sigma) was added to the cell culture media for 1 h before DNA isolation. .. One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology).

    Labeling:

    Article Title:
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in a Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title:
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA (~5 ng), 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in an Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Purification:

    Article Title:
    Article Snippet: .. For AccuStart HiFi reactions, the standard aPCR reaction was set using 1x AccuStart HiFi buffer (60 mM Tris-SO4 pH 8.9 at 25 °C, 18 mM (NH4 )2 SO4 ) with 2 mM MgSO4 , 200 µM dNTPs, 1 µM forward primer, 20 nM reverse primer, and 30 ng template DNA (single-stranded M13mp18 genomic DNA from NEB or synthetic double stranded gBlock from Integrated DNA Technologies, Gibson assembled dsDNA , or Phusion generated and purified double stranded DNA template), brought to 50 µL with nuclease-free water (IDT), with 0.22 µL AccuStart HiFi enzyme added to begin synthesis. ..

    IA:

    Article Title:
    Article Snippet: .. Following optimization, the final LAMP reaction mix (25 μl) for stx 1 and stx 2 consisted of 1× ThermoPol reaction buffer (New England BioLabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM F3 and B3 (Integrated DNA Technologies, Coralville, IA), 1.8 μM FIP and BIP, 1 μM LF and LB, 10 U of Bst DNA polymerase (New England BioLabs), and 2 μl of DNA template. ..

    Article Title:
    Article Snippet: .. The core reagent mix in a total volume of 25 μl contained 1× ThermoPol reaction buffer (New England Biolabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM F3 and B3 (Integrated DNA Technologies, Coralville, IA), 1.8 μM FIP and BIP, 1 μM Loop-F and Loop-B, 100 μg/ml luciferin potassium salt (Sigma-Aldrich, St. Louis, MO), 0.25 mM APS (Sigma-Aldrich), 0.5 U/ml ATP sulfurylase (New England Biolabs), 5.6 μg/ml Ultra-Glo firefly luciferase (Promega, Madison, WI), 10 U of Bst DNA polymerase (New England Biolabs), and 2 μl of DNA template (S . ..

    Article Title:
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Article Title:
    Article Snippet: .. Briefly, the LAMP reagent mix (25 μl) contained 1× ThermoPol reaction buffer (New England BioLabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM each outer primer (Integrated DNA Technologies, Coralville, IA), 1.8 μM each inner primer, 1 μM each loop primer, 10 U of Bst DNA polymerase (New England BioLabs), and 2 μl of template DNA. .. All LAMP reactions were carried out at 65°C (63°C for the O157 LAMP) for 1 h and terminated at 80°C for 5 min in an LA-320C real-time turbidimeter (Eiken Chemical Co., Ltd., Tokyo, Japan).

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in a Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title:
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA (~5 ng), 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in an Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title:
    Article Snippet: .. One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology). ..

    Article Title:
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Generated:

    Article Title:
    Article Snippet: .. For AccuStart HiFi reactions, the standard aPCR reaction was set using 1x AccuStart HiFi buffer (60 mM Tris-SO4 pH 8.9 at 25 °C, 18 mM (NH4 )2 SO4 ) with 2 mM MgSO4 , 200 µM dNTPs, 1 µM forward primer, 20 nM reverse primer, and 30 ng template DNA (single-stranded M13mp18 genomic DNA from NEB or synthetic double stranded gBlock from Integrated DNA Technologies, Gibson assembled dsDNA , or Phusion generated and purified double stranded DNA template), brought to 50 µL with nuclease-free water (IDT), with 0.22 µL AccuStart HiFi enzyme added to begin synthesis. ..

    Luciferase:

    Article Title:
    Article Snippet: .. The core reagent mix in a total volume of 25 μl contained 1× ThermoPol reaction buffer (New England Biolabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM F3 and B3 (Integrated DNA Technologies, Coralville, IA), 1.8 μM FIP and BIP, 1 μM Loop-F and Loop-B, 100 μg/ml luciferin potassium salt (Sigma-Aldrich, St. Louis, MO), 0.25 mM APS (Sigma-Aldrich), 0.5 U/ml ATP sulfurylase (New England Biolabs), 5.6 μg/ml Ultra-Glo firefly luciferase (Promega, Madison, WI), 10 U of Bst DNA polymerase (New England Biolabs), and 2 μl of DNA template (S . ..

    Lamp Assay:

    Article Title:
    Article Snippet: Each LAMP assay employed five to six specially designed LAMP primers (see Table S1 in the supplemental material), two outer primers, two inner primers, and one or two loop primers that recognized specific regions of the target DNA sequences. .. Briefly, the LAMP reagent mix (25 μl) contained 1× ThermoPol reaction buffer (New England BioLabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM each outer primer (Integrated DNA Technologies, Coralville, IA), 1.8 μM each inner primer, 1 μM each loop primer, 10 U of Bst DNA polymerase (New England BioLabs), and 2 μl of template DNA.

    Touchdown PCR:

    Article Title:
    Article Snippet: PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Sequencing:

    Article Title:
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Nested PCR:

    Article Title:
    Article Snippet: First round PCR Amplification of mtDNA was performed using a two step nested PCR protocol to avoid amplification of homologous regions in the nuclear DNA. .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl.

    Gel Purification:

    Article Title:
    Article Snippet: PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. Samples were loaded onto a 1.5% agarose gel and run for 25 min at 120 V. Subsequent treatment with ethidium bromide allowed visualization of the PCR product and gel purification.

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  • Team
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  • Bioz Stars
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  • 93
    Integrated DNA Technologies mgso4
    Mgso4, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgso4/product/Integrated DNA Technologies
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2020-01
    93/100 stars
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