mgso4  (Integrated DNA Technologies)


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    Structured Review

    Integrated DNA Technologies mgso4
    Mgso4, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgso4/product/Integrated DNA Technologies
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2020-08
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: Molecular genetic diagnostic techniques in choroideremia
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    IA:

    Article Title: Loop-Mediated Isothermal Amplification Assays for Detecting Shiga Toxin-Producing Escherichia coli in Ground Beef and Human Stools
    Article Snippet: .. Following optimization, the final LAMP reaction mix (25 μl) for stx 1 and stx 2 consisted of 1× ThermoPol reaction buffer (New England BioLabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM F3 and B3 (Integrated DNA Technologies, Coralville, IA), 1.8 μM FIP and BIP, 1 μM LF and LB, 10 U of Bst DNA polymerase (New England BioLabs), and 2 μl of DNA template. ..

    Article Title: Molecular genetic diagnostic techniques in choroideremia
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Article Title: Evaluation of a Loop-Mediated Isothermal Amplification Suite for the Rapid, Reliable, and Robust Detection of Shiga Toxin-Producing Escherichia coli in Produce
    Article Snippet: .. Briefly, the LAMP reagent mix (25 μl) contained 1× ThermoPol reaction buffer (New England BioLabs, Ipswich, MA), 6 mM MgSO4 , 1.2 mM each deoxynucleoside triphosphate (dNTP), 0.1 μM each outer primer (Integrated DNA Technologies, Coralville, IA), 1.8 μM each inner primer, 1 μM each loop primer, 10 U of Bst DNA polymerase (New England BioLabs), and 2 μl of template DNA. .. All LAMP reactions were carried out at 65°C (63°C for the O157 LAMP) for 1 h and terminated at 80°C for 5 min in an LA-320C real-time turbidimeter (Eiken Chemical Co., Ltd., Tokyo, Japan).

    Labeling:

    Article Title: Is detection of intraperitoneal exfoliated tumor cells after surgical resection of rectal cancer a prognostic factor of survival?
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in a Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title: Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture micro-dissection and cycling temperature capillary electrophoresis
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA (~5 ng), 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in an Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Purification:

    Article Title: In vitro synthesis of gene-length single-stranded DNA
    Article Snippet: .. For AccuStart HiFi reactions, the standard aPCR reaction was set using 1x AccuStart HiFi buffer (60 mM Tris-SO4 pH 8.9 at 25 °C, 18 mM (NH4 )2 SO4 ) with 2 mM MgSO4 , 200 µM dNTPs, 1 µM forward primer, 20 nM reverse primer, and 30 ng template DNA (single-stranded M13mp18 genomic DNA from NEB or synthetic double stranded gBlock from Integrated DNA Technologies, Gibson assembled dsDNA , or Phusion generated and purified double stranded DNA template), brought to 50 µL with nuclease-free water (IDT), with 0.22 µL AccuStart HiFi enzyme added to begin synthesis. ..

    Polymerase Chain Reaction:

    Article Title: Is detection of intraperitoneal exfoliated tumor cells after surgical resection of rectal cancer a prognostic factor of survival?
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA, 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in a Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title: Mapping mitochondrial heteroplasmy in a Leydig tumor by laser capture micro-dissection and cycling temperature capillary electrophoresis
    Article Snippet: .. The PCR reaction mixture contained 0.1 μl of extracted DNA (~5 ng), 0.8 mM dNTPs (0.2 mM of each dNTP) (VWR, Oslo, Norway), 1X Thermopol Buffer, 2 mM MgSO4 , 0.075unit Taq/μl, 0.15 μM of each forward, reverse and fluorescently labeled primer (Integrated DNA Technologies, Leuven, Belgium) and total reaction volume of 10 μl. .. The temperature cycling was performed in an Eppendorf Mastercycler ep gradient S (Eppendorf, Hamburg, Germany) with an initial denaturation 94 °C for 240 s followed by cycling 38 times under the following conditions, denaturation at 94 °C for 15 s, annealing for 40 s with temperature given in Table and elongation at 72 °C for 150 s.

    Article Title: Structure, subunit organization and behavior of the asymmetric Type IIT restriction endonuclease BbvCI
    Article Snippet: .. PCR reactions were performed in 100 μl volumes containing 72.5 μl dH2 O; 10 microliters 10× ThermoPol buffer (final concentrations: 20 mM Tris–HCl, 10 mM KCl, 10 mM (NH4 )2 SO4 , 2 mM MgSO4 , 0.1% Triton X-100, pH 8.8 @ 25°C); 5 μl (0.5 μg) template DNA, 5 μl (0.6 μg) of each primer (IDT); and 2.5 μl (5 units) of Deep Vent Exo+ polymerase (NEB). .. Amplifications were carried out in an Applied Biosystems 2720 thermocycler, typically using the following protocol: 94°C 1 min; 25 cycles of (94°C 30 s; 56°C 30 s; 72°C 1 min); 72°C 7 min; 4°C.

    Article Title: Asymmetric bidirectional replication at the human DBF4 origin
    Article Snippet: .. One-way PCR reactions were carried out in a final volume of 30 μl containing 1×PCR buffer (ID Labs Biotechnology), 200 μM dNTPs, 3 mM MgSO4 , 400 nM digoxigenin-labeled oligonucleotides (Integrated DNA Technologies), 1 M betaine (Sigma) and 1 unit of IDPol DNA polymerase (ID Labs Biotechnology). ..

    Article Title: Molecular genetic diagnostic techniques in choroideremia
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

    Generated:

    Article Title: In vitro synthesis of gene-length single-stranded DNA
    Article Snippet: .. For AccuStart HiFi reactions, the standard aPCR reaction was set using 1x AccuStart HiFi buffer (60 mM Tris-SO4 pH 8.9 at 25 °C, 18 mM (NH4 )2 SO4 ) with 2 mM MgSO4 , 200 µM dNTPs, 1 µM forward primer, 20 nM reverse primer, and 30 ng template DNA (single-stranded M13mp18 genomic DNA from NEB or synthetic double stranded gBlock from Integrated DNA Technologies, Gibson assembled dsDNA , or Phusion generated and purified double stranded DNA template), brought to 50 µL with nuclease-free water (IDT), with 0.22 µL AccuStart HiFi enzyme added to begin synthesis. ..

    Sequencing:

    Article Title: Molecular genetic diagnostic techniques in choroideremia
    Article Snippet: .. PCR amplification and sequencing using genomic DNA For a 50 µl reaction, the following reagents were included: 10 µl of 10X Pfx Amplification Buffer ( Invitrogen , Burlington, ON), 1.5 µl of 10 mM dNTP mix, 0.75 µl of 50 mM MgSO4 , 1.5 µl each of 10 µM sense and antisense primer ( Integrated DNA Technologies , Coralville, IA; ), 0.4 µl of Platinum Pfx DNA polymerase (Invitrogen), 100 ng of genomic DNA template, and sterile H2 O. .. A touchdown PCR protocol was used in a thermal cycler (BioRad).

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  • News
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  • Team
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  • Bioz Stars
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  • 92
    Integrated DNA Technologies mgso4
    Mgso4, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgso4/product/Integrated DNA Technologies
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    mgso4 - by Bioz Stars, 2020-08
    92/100 stars
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