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PerkinElmer mgcl
Mgcl, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl/product/PerkinElmer
Average 96 stars, based on 9 article reviews
Price from $9.99 to $1999.99
mgcl - by Bioz Stars, 2020-07
96/100 stars

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Incubation:

Article Title: In Vitro Epsilon RNA-Dependent Protein Priming Activity of Human Hepatitis B Virus Polymerase
Article Snippet: .. One microliter of [α-32 P]dGTP (10 mCi/ml [3,000 Ci/mmol]; PerkinElmer) was then added, and the reaction mixtures were incubated at 25°C for 4 h with shaking. ..

Article Title: RNA/DNA hybrid binding affinity determines telomerase template-translocation efficiency
Article Snippet: .. The single-translocation reaction was initiated by adding 20 μl of incubated enzyme–primer mixture and 20 μl of pre-chilled nucleotide mixture containing 2 mM dATP, 4 μM dGTP, 0.66 μM α-32 P-dGTP (3000 Ci/mmol, 10 mCi/ml, Perkin-Elmer) and 2 μM competitive DNA primer 5′-(TTAGGG)3 -3′-amine in 1 × low-Mg2+ telomerase reaction buffer. .. Reaction was carried out on ice to slow down enzyme kinetics.

Activity Assay:

Article Title: Telomeric G-quadruplexes are a substrate and site of localization for human telomerase
Article Snippet: .. Telomerase activity assays The following reaction was prepared to give 15 μl per sample: between 1 nM and 2 μM of the specified oligonucleotide, 50 mM Tris-HCl pH 8.5, 1 mM MgCl2 , 5 mM DTT, 1 mM spermidine-HCl, 0.5 mM dTTP, 0.5 mM dATP, 4.6 μM non-radioactive dGTP and 0.33 μM [α-32 P]dGTP at 20 mCi ml−1 , 6,000 Ci mmol−1 (PerkinElmer Life Sciences), 10% glycerol and either 150 mM KCl for experiments with 7GGT or 2.5 mM SrCl2 for experiments with 22GGG. .. The reaction was initiated by adding 5 μl of purified human telomerase, and incubating at 30 °C for 1 h. The reaction was quenched by the addition of 80 μl of stop-buffer (50 mM Tris-HCl, pH 8.3, 20 mM EDTA and 0.2% SDS) and 1–2 × 103 c.p.m. of a 5′-32 P-labelled synthetic 100-mer DNA as an internal recovery standard.

In Vitro:

Article Title: RNA/DNA hybrid binding affinity determines telomerase template-translocation efficiency
Article Snippet: .. In brief, 2–3 μl of in vitro reconstituted telomerase was assayed in a 10-μl reaction containing 1 × telomerase reaction buffer, 1 mM dTTP, 1 mM dATP, 2 μM dGTP, 0.165 μM α-32 P-dGTP (3000 Ci/mmol, 10 mCi/ml, Perkin-Elmer) and 1 μM (TTAGGG)3 DNA primer. .. The reaction was incubated at 30°C for 60 min and terminated by phenol/chloroform extraction followed by ethanol precipitation.

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    PerkinElmer mgcl
    Mgcl, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl/product/PerkinElmer
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mgcl - by Bioz Stars, 2020-07
    92/100 stars
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