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Millipore mgcl
Mgcl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl/product/Millipore
Average 99 stars, based on 13 article reviews
Price from $9.99 to $1999.99
mgcl - by Bioz Stars, 2020-07
99/100 stars

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Related Articles

Microelectrode Array:

Article Title: STED and STORM Superresolution Imaging of Primary Cilia
Article Snippet: .. Imaging buffer for oxygen scavenging: (a) Glucose (Sigma-Aldrich, G5767). (b) Glucose oxidase (Sigma-Aldrich, G2133). (c) Catalase (Sigma-Aldrich, C40). (d) Mercaptoethylamine (MEA or Cysteamine, Sigma-Aldrich, 30070). (e) TN buffer : Tris–HCI, pH 8.0 (10 mM), NaCl (150 mM). .. Secondary antibodies: Oregon Green 488 conjugated IgG(Life Technologies) and biotin conjugated antibody (Sigma-Aldrich).

other:

Article Title: Srs2 prevents Rad51 filament formation by repetitive scrunching of DNA
Article Snippet: Standard Rad51 reaction buffer was 50 mM Tris-HCl (pH 7.5), 50 mM NaCl, 10 mM MgCl2 ; standard RecA reaction buffer was 100 mM NaAc, 25 mM Tris-HCl (pH 7.5), 10 mM MgAc, with an oxygen scavenging system containing 0.8% v/v dextrose, 1 mg/ml glucose oxidase, 0.03 mg/ml catalase , and 2-mercaptoethanol (1% v/v), all items were purchased from Sigma Aldrich (St. Louis, MO).

Imaging:

Article Title: NAP1L1 accelerates activation and decreases pausing to enhance nucleosome remodeling by CSB
Article Snippet: .. Single-molecule experiments were performed in an imaging buffer (12 mM HEPES pH 7.9, 10 mM Tris–HCl pH 7.5, 60 mM KCl, 4 mM MgCl2 , 3% glycerol) containing a gloxy oxygen scavenging system (a mixture of glucose oxidase (1 mg/ml, Sigma), catalase (0.04 mg/ml, Sigma), glucose (0.4% (w/v), Sigma), and Trolox (2 mM, Sigma)). .. Cy3 and Cy5 were alternatively excited with 532 and 633 nm lasers using the ALEX (Alternative Laser EXcitation) technique ( , ), and single-molecule fluorescence images were synchronously taken with the excitation laser switching at a frame rate of 10 Hz using a home-built prism-type total internal reflection fluorescence microscope equipped with an electron-multiplying charge coupled device camera (Ixon DV897; Andor Technology).

Article Title: p53 Dynamically Directs TFIID Assembly on Target Gene Promoters
Article Snippet: .. An oxygen-scavenging system consisting of 0.4% b- d -glucose, 1 mg/ml glucose oxidase (Sigma), and 0.4 mg/ml catalase (Sigma) was used for imaging. .. For experiments designed to measure p53's interaction with promoter DNA, Alexa Fluor 555- or Cy3- or Cy5-labeled p53 (8 nM, monomer) was added in the absence or presence of unlabeled TFIID (1 nM) to the flow cell containing surfaced-attached mapped promoter DNA and immediately imaged at 3 Hz.

Article Title: STED and STORM Superresolution Imaging of Primary Cilia
Article Snippet: .. Imaging buffer for oxygen scavenging: (a) Glucose (Sigma-Aldrich, G5767). (b) Glucose oxidase (Sigma-Aldrich, G2133). (c) Catalase (Sigma-Aldrich, C40). (d) Mercaptoethylamine (MEA or Cysteamine, Sigma-Aldrich, 30070). (e) TN buffer : Tris–HCI, pH 8.0 (10 mM), NaCl (150 mM). .. Secondary antibodies: Oregon Green 488 conjugated IgG(Life Technologies) and biotin conjugated antibody (Sigma-Aldrich).

Article Title: Effect of Single-Strand Break on Branch Migration and Folding Dynamics of Holliday Junctions
Article Snippet: .. A 25 pM solution of biotinylated HJs in TES buffer was added and incubated for 10 min. Measurements were performed in imaging buffer containing 10 mM Tris-HCl (pH 7.5–7.8), 0.4% (w/v) glucose, an oxygen-scavenging mixture of glucose oxidase (3430 U/100 μ L; Sigma-Aldrich) and catalase (52,000 U/100 μ L; Sigma-Aldrich/Fluka), and 1.5–2 mM Trolox (Sigma-Aldrich) as a free-radicals quencher ( ). .. Also, the imaging buffer contained varying amounts of MgCl2 according to the desired experimental conditions.

Incubation:

Article Title: A New Method for Inferring Hidden Markov Models from Noisy Time Sequences
Article Snippet: .. Holliday junctions (50 pM molecules) were added to the channel and incubated for 5 minutes before washing with T50 buffer supplemented with MgCl (as stated), an oxygen scavenger system (1 mg/ml glucose oxidase, 0.04 mg/ml catalase, and 0.8 mg/ml dextrose, Sigma) and anti-photobleaching reagents (1 mM methylviologen, 1 mM Ascorbic Acid, Sigma) . .. FRET spectra were obtained using a custom built objective-based total-internal-reflection fluorescence (TIRF) microscope which is very similar in design to one described in detail elsewhere .

Article Title: Effect of Single-Strand Break on Branch Migration and Folding Dynamics of Holliday Junctions
Article Snippet: .. A 25 pM solution of biotinylated HJs in TES buffer was added and incubated for 10 min. Measurements were performed in imaging buffer containing 10 mM Tris-HCl (pH 7.5–7.8), 0.4% (w/v) glucose, an oxygen-scavenging mixture of glucose oxidase (3430 U/100 μ L; Sigma-Aldrich) and catalase (52,000 U/100 μ L; Sigma-Aldrich/Fluka), and 1.5–2 mM Trolox (Sigma-Aldrich) as a free-radicals quencher ( ). .. Also, the imaging buffer contained varying amounts of MgCl2 according to the desired experimental conditions.

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    Millipore mgcl
    Mgcl, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl/product/Millipore
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mgcl - by Bioz Stars, 2020-07
    99/100 stars
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