Structured Review

Meridian Life Science mgcl
Mgcl, supplied by Meridian Life Science, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl/product/Meridian Life Science
Average 92 stars, based on 9 article reviews
Price from $9.99 to $1999.99
mgcl - by Bioz Stars, 2020-07
92/100 stars

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Polymerase Chain Reaction:

Article Title: The evolution of imprinting: chromosomal mapping of orthologues of mammalian imprinted domains in monotreme and marsupial mammals
Article Snippet: .. GNAS , GRB10 and IGF2R were amplified in a 25 μl reaction containing 1× PCR Buffer (Bioline), 1.5 mM MgCl, 250 μM dNTPs, 1.5 U Taq polymerase (Bioline), 0.3 μM of each p rimer and 50 ng gDNA. .. PCR cycling was, 94°C for 5 min, 35 cycles at 94°C for 30 sec, annealing temperature (specific for each primer see table ) for 30 sec, 72°C for 30 sec and 5 min at 72°C.

Article Title: Tracing the Tiger: Population Genetics Provides Valuable Insights into the Aedes (Stegomyia) albopictus Invasion of the Australasian Region
Article Snippet: .. The final PCR mixture contained 1× Mytaq buffer (Bioline)(containing pre-optimized concentrations of MgCl and dNTPs), 0.4 µM of each primer, 0.5–1.0 unit of MyTaq polymerase (Bioline) and 5.0–10.0 ng of extracted genomic DNA (1 µl of extraction). ..

Article Title: A phylogenetic analysis of the Primnoidae (Anthozoa: Octocorallia: Calcaxonia) with analyses of character evolution and a key to the genera and subgenera
Article Snippet: .. PCR amplifications were performed in 10 μL reactions with 1 μL of unquantified genomic DNA and final concentrations of 3 pmol of each primer, 500 μM dNTPS, 3 mM MgCl, 0.25 mg/μL (0.25 μL of 10 mg/μL stock) bovine serum albumin (BSA), and 0.05 U/μL of Biolase™ DNA polymerase (Bioline, Inc) with manufacturer provided buffers. .. Thermal cycler parameters included an initial denaturing step of 94 °C for 3 min, followed by 35 or 40 rounds of 94 °C for 30 s, 50 °C for 30 or 60 s, 74 °C for 60 s, and a final extension step of 72 °C for 5 min. ExoSAP-IT® (Affymetrix), diluted to a 1:3 concentration of enzyme to purified water and run using a heat profile of 37 °C for 30 min followed by 80 °C for 20 min, was used to neutralize the PCR products.

Amplification:

Article Title: The evolution of imprinting: chromosomal mapping of orthologues of mammalian imprinted domains in monotreme and marsupial mammals
Article Snippet: .. GNAS , GRB10 and IGF2R were amplified in a 25 μl reaction containing 1× PCR Buffer (Bioline), 1.5 mM MgCl, 250 μM dNTPs, 1.5 U Taq polymerase (Bioline), 0.3 μM of each p rimer and 50 ng gDNA. .. PCR cycling was, 94°C for 5 min, 35 cycles at 94°C for 30 sec, annealing temperature (specific for each primer see table ) for 30 sec, 72°C for 30 sec and 5 min at 72°C.