Structured Review

Merck KGaA mgcl
Mgcl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl/product/Merck KGaA
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mgcl - by Bioz Stars, 2020-07
90/100 stars

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Produced:

Article Title: Structural and functional studies of the glycoside hydrolase family 3 β-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii
Article Snippet: .. Crystallization drops were produced by mixing 1 µl 18.9 mg ml−1 protein solution in 25 mM bis-tris propane (Sigma–Aldrich, USA) pH 7.5 with 1 µl reservoir solution consisting of 0.15 M MgCl.6H2 O (Merck Millipore, Germany), 16%(w /v ) polyethylene glycol (PEG) 3350 (Hampton Research, USA). ..

Lysis:

Article Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
Article Snippet: .. For purification of full length constructs containing a TEV cleavable Z-basic-his tag, cell pellets were thawed and resuspended in lysis buffer (100 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 10 mM imidazole, 1 mM Tris (2-carboxyethyl) phosphene (TCEP), 0.1% DDM, 1 mM MgCl, 1 x set III protease inhibitors (Merck, Germany). .. Cells were lysed by sonication and 1 unit of Benzonase was added to lysates before the cell were debris pelleted by centrifugation.

Crystallization Assay:

Article Title: Structural and functional studies of the glycoside hydrolase family 3 β-glucosidase Cel3A from the moderately thermophilic fungus Rasamsonia emersonii
Article Snippet: .. Crystallization drops were produced by mixing 1 µl 18.9 mg ml−1 protein solution in 25 mM bis-tris propane (Sigma–Aldrich, USA) pH 7.5 with 1 µl reservoir solution consisting of 0.15 M MgCl.6H2 O (Merck Millipore, Germany), 16%(w /v ) polyethylene glycol (PEG) 3350 (Hampton Research, USA). ..

Construct:

Article Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
Article Snippet: .. For purification of full length constructs containing a TEV cleavable Z-basic-his tag, cell pellets were thawed and resuspended in lysis buffer (100 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 10 mM imidazole, 1 mM Tris (2-carboxyethyl) phosphene (TCEP), 0.1% DDM, 1 mM MgCl, 1 x set III protease inhibitors (Merck, Germany). .. Cells were lysed by sonication and 1 unit of Benzonase was added to lysates before the cell were debris pelleted by centrifugation.

Purification:

Article Title: SPRTN is a mammalian DNA-binding metalloprotease that resolves DNA-protein crosslinks
Article Snippet: .. For purification of full length constructs containing a TEV cleavable Z-basic-his tag, cell pellets were thawed and resuspended in lysis buffer (100 mM HEPES pH 7.5, 500 mM NaCl, 10% glycerol, 10 mM imidazole, 1 mM Tris (2-carboxyethyl) phosphene (TCEP), 0.1% DDM, 1 mM MgCl, 1 x set III protease inhibitors (Merck, Germany). .. Cells were lysed by sonication and 1 unit of Benzonase was added to lysates before the cell were debris pelleted by centrifugation.

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  • 90
    Merck KGaA mgcl
    Mgcl, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    92
    Merck KGaA mgcl ∙ 6h2 o
    Mgcl ∙ 6h2 O, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl ∙ 6h2 o/product/Merck KGaA
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mgcl ∙ 6h2 o - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

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