mgcl buffer  (Millipore)


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  • 99
    Name:
    Tris Borate EDTA buffer
    Description:

    Catalog Number:
    t3913
    Price:
    None
    Applications:
    TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. Dilution of the TBE stock concentrates to a 1x TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5x or 10x stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
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    Structured Review

    Millipore mgcl buffer
    Tris Borate EDTA buffer

    https://www.bioz.com/result/mgcl buffer/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl buffer - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Nucleic Acid Electrophoresis:

    Article Title: Application of Polymerase Chain Reaction to Detect Burkholderia Pseudomallei and Brucella Species in Buffy Coat from Patients with Febrile Illness Among Rural and Peri-Urban Population
    Article Snippet: .. Analysis of amplification products An aliquot of 5 μL amplicon was analyzed by gel electrophoresis in 2% agarose (Sigma, MO, USA) prepared in Tris-Borate-EDTA buffer containing 0.5μg/mL of ethidium bromide (Sigma, MO, USA). ..

    Amplification:

    Article Title: Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane
    Article Snippet: .. The amplification products were resolved on 12% acrylamide gel with 1 × Tris borate EDTA buffer, stained with 0.5 µg/ml ethidium bromide (Sigma-Aldrich, USA) and images were captured using a gel documentation system (G:Box, Syngene, U.K.). .. Only clear and unambiguous amplified products were scored as dominant markers with 1 (present) or 0 (absent) score since it is difficult to identify and differentiate between amplicons from homologous chromosomes.

    Article Title: Application of Polymerase Chain Reaction to Detect Burkholderia Pseudomallei and Brucella Species in Buffy Coat from Patients with Febrile Illness Among Rural and Peri-Urban Population
    Article Snippet: .. Analysis of amplification products An aliquot of 5 μL amplicon was analyzed by gel electrophoresis in 2% agarose (Sigma, MO, USA) prepared in Tris-Borate-EDTA buffer containing 0.5μg/mL of ethidium bromide (Sigma, MO, USA). ..

    Mutagenesis:

    Article Title: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing
    Article Snippet: .. PCR samples were resolved on 0.5× acrylamide gels containing 12.5 ml MDE® (Mutation Detection Enhancement) gel solution (Lonza Group Ltd., Basel, Switzerland), 3 ml of 10× TBE (Tris/Borate/EDTA, pH 8.3) buffer, 34.28 ml deionised water, 20 μl tetramethylethylenediamine (TEMED; Sigma-Aldrich, St Louis, Missouri, UK) and 200 μl of freshly prepared 10% ammonium persulfate (APS; Sigma-Aldrich, St Louis, Missouri, UK). .. PCR samples were prepared for electrophoresis as follows; 3 μl of PCR product was mixed with 7 μl of denaturing loading buffer (95% formamide, 0.025% bromophenol blue, 0.025% xylene cyanol and 20 mM EDTA) (all reagents from Sigma-Aldrich, St Louis, Missouri, UK).

    Blocking Assay:

    Article Title: Steady-State Kinetic Analysis of DNA Polymerase Single-Nucleotide Incorporation Products
    Article Snippet: .. 40% Acrylamide / bis-acrylamide solution (19:1, w/w (5% crosslinker), electrophoresis purity reagent) (Bio-Rad Laboratories) Ammonium persulfate (Bio-Rad Laboratories) Aqueous ethanol (70% v/v, reagent grade) Bovine serum albumin (BSA), standard solution (2 mg/mL) (Pierce Protein Biology Products) Bromophenol blue (SigmaAldrich) 2’-Deoxyoribonucleoside triphosphate solutions (dNTP, 100 mM; New England Biolabs) D,L-Dithiothreitol (DTT) (Research Products International) DNA template (see Strategic Planning) Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA, SigmaAldrich) Formamide (Roche) Glycerol (SigmaAldrich) Human DNA polymerase η (hpol η; see Strategic Planning) Magnesium chloride solution (25 mM) (Applied Biosystems) N , N ,Ń,Ń-Tetramethylethylenediamine (TEMED; Bio-Rad Laboratories) Potassium chloride (SigmaAldrich) Primer (see Strategic Planning) Siliconizing reagent for glass (e.g. Sigmacote®; SigmaAldrich) Tris-HCl buffer (1 M), pH 7.5 (Trizma® hydrochloride buffer solution, SigmaAldrich) 10 X Tris-Borate-EDTA (TBE) buffer (0.89 M Tris·Cl, 0.89 M boric acid, 20 mM EDTA) (individual solid components, SigmaAldrich) Urea (electrophoresis grade, SigmaAldrich) Xylene cyanol FF (SigmaAldrich) Centrifuge Centrifuge tube holder (UNITED Laboratory Plastics) Cling plastic wrap Digital timer (model # 62344-641, VWR International) Disposable needle (18 G) Disposable syringe (25 mL) Dry block heater (e.g. VWR International, model # 946310) fitted with a modular heating block (e.g. VWR International, 30 wells to hold 0.65 mL centrifuge tubes) and thermometer (e.g. VWR International). .. Erlenmeyer flask (125 mL) Flat end sequencing tips (0.37 mm, 1-200 μL; Phenix Research Products) Graduated cylinder (5 L) GraphPad Prism® software (GraphPad Software) Image J software (National Institutes of Health) Lint-free tissue (e.g. Kimwipes, Kimberly-Clark) Magnetic stir plate and stir bar Microcentrifuge tubes (0.65 mL, VWR International) Microsoft ExcelTM (Microsoft Corp.) Nucleic acid Electrophoresis standard vertical apparatus (e.g. Sequi-Gen® GT DNA electrophoresis cell, 38 × 50 cm (Bio-Rad Laboratories)) Single edge razor blade or scalpel Standard set of micropipettors (1000 μL, 200 μL, 20 μL, 10 μL, and 2 μL) with disposable tips Typhoon Trio Variable Mode Imager (GE Healthcare Life Sciences) Vortex mixing device

    Electrophoresis:

    Article Title: Internally Cationic Polyamidoamine PAMAM-OH Dendrimers for siRNA Delivery: Effect of the Degree of Quaternization and Cancer Targeting
    Article Snippet: .. PCR products were separated in 4% NuSieve 3:1 Reliant-agarose gels (Lonza, Basel, Switzerland) in 1x Tris-borate EDTA buffer (0.089 mol/L Tris-borate, 0.002 mol/L EDTA, pH 8.3; Research Organics Inc., Cleveland, OH) by submarine electrophoresis. .. The gels were stained with ethidium bromide and digitally photographed.

    Article Title: Steady-State Kinetic Analysis of DNA Polymerase Single-Nucleotide Incorporation Products
    Article Snippet: .. 40% Acrylamide / bis-acrylamide solution (19:1, w/w (5% crosslinker), electrophoresis purity reagent) (Bio-Rad Laboratories) Ammonium persulfate (Bio-Rad Laboratories) Aqueous ethanol (70% v/v, reagent grade) Bovine serum albumin (BSA), standard solution (2 mg/mL) (Pierce Protein Biology Products) Bromophenol blue (SigmaAldrich) 2’-Deoxyoribonucleoside triphosphate solutions (dNTP, 100 mM; New England Biolabs) D,L-Dithiothreitol (DTT) (Research Products International) DNA template (see Strategic Planning) Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA, SigmaAldrich) Formamide (Roche) Glycerol (SigmaAldrich) Human DNA polymerase η (hpol η; see Strategic Planning) Magnesium chloride solution (25 mM) (Applied Biosystems) N , N ,Ń,Ń-Tetramethylethylenediamine (TEMED; Bio-Rad Laboratories) Potassium chloride (SigmaAldrich) Primer (see Strategic Planning) Siliconizing reagent for glass (e.g. Sigmacote®; SigmaAldrich) Tris-HCl buffer (1 M), pH 7.5 (Trizma® hydrochloride buffer solution, SigmaAldrich) 10 X Tris-Borate-EDTA (TBE) buffer (0.89 M Tris·Cl, 0.89 M boric acid, 20 mM EDTA) (individual solid components, SigmaAldrich) Urea (electrophoresis grade, SigmaAldrich) Xylene cyanol FF (SigmaAldrich) Centrifuge Centrifuge tube holder (UNITED Laboratory Plastics) Cling plastic wrap Digital timer (model # 62344-641, VWR International) Disposable needle (18 G) Disposable syringe (25 mL) Dry block heater (e.g. VWR International, model # 946310) fitted with a modular heating block (e.g. VWR International, 30 wells to hold 0.65 mL centrifuge tubes) and thermometer (e.g. VWR International). .. Erlenmeyer flask (125 mL) Flat end sequencing tips (0.37 mm, 1-200 μL; Phenix Research Products) Graduated cylinder (5 L) GraphPad Prism® software (GraphPad Software) Image J software (National Institutes of Health) Lint-free tissue (e.g. Kimwipes, Kimberly-Clark) Magnetic stir plate and stir bar Microcentrifuge tubes (0.65 mL, VWR International) Microsoft ExcelTM (Microsoft Corp.) Nucleic acid Electrophoresis standard vertical apparatus (e.g. Sequi-Gen® GT DNA electrophoresis cell, 38 × 50 cm (Bio-Rad Laboratories)) Single edge razor blade or scalpel Standard set of micropipettors (1000 μL, 200 μL, 20 μL, 10 μL, and 2 μL) with disposable tips Typhoon Trio Variable Mode Imager (GE Healthcare Life Sciences) Vortex mixing device

    Article Title: Inhibitory Effect of Substances Produced by Native Lactococcus lactis Strains of Tropical Fruits towards Food Pathogens
    Article Snippet: .. The PCR products were separated by electrophoresis on 1.2% agarose gels in 1× Tris-borate EDTA (TBE, pH 8.0) buffer (Sigma- Aldrich Co.). .. Gels were stained in TBE buffer containing 0.5 μg/mL ethidium bromide (Sigma-Aldrich Co.).

    Polymerase Chain Reaction:

    Article Title: Detection of three closely located single nucleotide polymorphisms in the EAAT2 promoter: comparison of single-strand conformational polymorphism (SSCP), pyrosequencing and Sanger sequencing
    Article Snippet: .. PCR samples were resolved on 0.5× acrylamide gels containing 12.5 ml MDE® (Mutation Detection Enhancement) gel solution (Lonza Group Ltd., Basel, Switzerland), 3 ml of 10× TBE (Tris/Borate/EDTA, pH 8.3) buffer, 34.28 ml deionised water, 20 μl tetramethylethylenediamine (TEMED; Sigma-Aldrich, St Louis, Missouri, UK) and 200 μl of freshly prepared 10% ammonium persulfate (APS; Sigma-Aldrich, St Louis, Missouri, UK). .. PCR samples were prepared for electrophoresis as follows; 3 μl of PCR product was mixed with 7 μl of denaturing loading buffer (95% formamide, 0.025% bromophenol blue, 0.025% xylene cyanol and 20 mM EDTA) (all reagents from Sigma-Aldrich, St Louis, Missouri, UK).

    Article Title: Internally Cationic Polyamidoamine PAMAM-OH Dendrimers for siRNA Delivery: Effect of the Degree of Quaternization and Cancer Targeting
    Article Snippet: .. PCR products were separated in 4% NuSieve 3:1 Reliant-agarose gels (Lonza, Basel, Switzerland) in 1x Tris-borate EDTA buffer (0.089 mol/L Tris-borate, 0.002 mol/L EDTA, pH 8.3; Research Organics Inc., Cleveland, OH) by submarine electrophoresis. .. The gels were stained with ethidium bromide and digitally photographed.

    Article Title: Inhibitory Effect of Substances Produced by Native Lactococcus lactis Strains of Tropical Fruits towards Food Pathogens
    Article Snippet: .. The PCR products were separated by electrophoresis on 1.2% agarose gels in 1× Tris-borate EDTA (TBE, pH 8.0) buffer (Sigma- Aldrich Co.). .. Gels were stained in TBE buffer containing 0.5 μg/mL ethidium bromide (Sigma-Aldrich Co.).

    Acrylamide Gel Assay:

    Article Title: Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane
    Article Snippet: .. The amplification products were resolved on 12% acrylamide gel with 1 × Tris borate EDTA buffer, stained with 0.5 µg/ml ethidium bromide (Sigma-Aldrich, USA) and images were captured using a gel documentation system (G:Box, Syngene, U.K.). .. Only clear and unambiguous amplified products were scored as dominant markers with 1 (present) or 0 (absent) score since it is difficult to identify and differentiate between amplicons from homologous chromosomes.

    Staining:

    Article Title: Identification of marker-trait associations for morphological descriptors and yield component traits in sugarcane
    Article Snippet: .. The amplification products were resolved on 12% acrylamide gel with 1 × Tris borate EDTA buffer, stained with 0.5 µg/ml ethidium bromide (Sigma-Aldrich, USA) and images were captured using a gel documentation system (G:Box, Syngene, U.K.). .. Only clear and unambiguous amplified products were scored as dominant markers with 1 (present) or 0 (absent) score since it is difficult to identify and differentiate between amplicons from homologous chromosomes.

    Clear Native PAGE:

    Article Title: Crystal structures of the Arabidopsis thaliana proliferating cell nuclear antigen 1 and 2 proteins complexed with the human p21 C-terminal segment
    Article Snippet: .. His At PCNA1, At PCNA1, His At PCNA2, At PCNA2, and the heterotrimer were separated by 4% native PAGE in 1× TBE buffer and electrotransferred onto a PVDF membrane (0.2 μm) (Millipore), as described previously. .. After the transfer, the membrane was blocked with PBS containing 10% (w/v) fat-free milk and 0.1% (v/v) Tween 20 for 1 h. The membrane was incubated with a tetra-His horseradish peroxidase antibody conjugate for 1 h at room temperature (Qiagen, dilution 1:1000).

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  • Team
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  • 99
    Millipore mgcl buffer
    Mgcl Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl buffer/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mgcl buffer - by Bioz Stars, 2020-07
    99/100 stars
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