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Roche mgcl 2
Mgcl 2, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl 2/product/Roche
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mgcl 2 - by Bioz Stars, 2020-07
91/100 stars

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Centrifugation:

Article Title: Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity
Article Snippet: .. Briefly, cells (≈5 × 106 ) collected by centrifugation after scraping, were homogenized on ice in 1 mL of 50 mM Tris, pH 7.5, 100 mM NaCl, 2 mM MgCl 2, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 10% glycerol, and Roche protease inhibitor mixture. .. After centrifugation (10,000 × g , 10 min), supernatants were incubated (1.5 h, 4 °C) with 40 μL of CL4B Sepharose beads (50% slurry, Amersham).

Amplification:

Article Title: High prevalence of Trypanosoma vegrandis in bats from Western Australia.
Article Snippet: .. PCR amplification with the T. vegrandis-specific primers was performed with the final mix containing 1 l of first round gel purified PCR product, 0.8 M of each primer, 0.02 U/l Kapa Taq (Kapa Biosystems, Massachusetts, U.S.A.), a final concentration of 2.5 mM of each dNTP and 2.5 l of 10× buffer (Kapa Biosystems, Massachusetts, U.S.A.) with 1.5 mM MgCl 2 (Kapa Biosystems, Massachusetts, U.S.A.) and Baxter Ultra-Pure H2O to a volume of 25 l. Cycling conditions consisted of an initial PCR round of 94 • C for 5 min, 50 • C for 2 min and 72 • C for 4 min followed by 45 cycles of 94 • C for 30 s, 60 • C for 30 s and 72 • C for 30 s. The PCR was completed with a final extension of 7 min at 72 • C. .. 18S rRNA amplification and sequencing For amplification of Trypanosoma spp. external forward primer S-LF and external reverse primer S-762 was used in the primary PCR while two pairs of internal primers S-825 and S-LIR (producing a ∼959 bp) and S-823 and S-662 (producing a ∼900 bp) were used in secondary reactions as previously described by Maslov et al. (1996) and McInnes et al. (2009) .

Article Title: A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.
Article Snippet: .. Amplification was performed in a Perkin-Elmer, Applied Biosystems 9700 thermocycler using 50 L reactions comprising 1× RT-PCR reaction buffer containing 1.5 mM MgCl 2 , 200 M dNTPs, 10 mM DTT, 20 U of RNasin, 0.2 M of each primer and 1 L of Titan enzyme mix (Roche). ..

Concentration Assay:

Article Title: High prevalence of Trypanosoma vegrandis in bats from Western Australia.
Article Snippet: .. PCR amplification with the T. vegrandis-specific primers was performed with the final mix containing 1 l of first round gel purified PCR product, 0.8 M of each primer, 0.02 U/l Kapa Taq (Kapa Biosystems, Massachusetts, U.S.A.), a final concentration of 2.5 mM of each dNTP and 2.5 l of 10× buffer (Kapa Biosystems, Massachusetts, U.S.A.) with 1.5 mM MgCl 2 (Kapa Biosystems, Massachusetts, U.S.A.) and Baxter Ultra-Pure H2O to a volume of 25 l. Cycling conditions consisted of an initial PCR round of 94 • C for 5 min, 50 • C for 2 min and 72 • C for 4 min followed by 45 cycles of 94 • C for 30 s, 60 • C for 30 s and 72 • C for 30 s. The PCR was completed with a final extension of 7 min at 72 • C. .. 18S rRNA amplification and sequencing For amplification of Trypanosoma spp. external forward primer S-LF and external reverse primer S-762 was used in the primary PCR while two pairs of internal primers S-825 and S-LIR (producing a ∼959 bp) and S-823 and S-662 (producing a ∼900 bp) were used in secondary reactions as previously described by Maslov et al. (1996) and McInnes et al. (2009) .

Protease Inhibitor:

Article Title: A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
Article Snippet: .. Generation of RIG-I Mutants Protein Purification and Analysis (His 6 )-Flag-tagged RIG-I(WT) and RIG-I(H830A) were transiently overexpressed in HEK293 blue cells and lysed in a CHAPS-containing lysis buffer (150 mM NaCl, 50 mM Tris/HCl [pH 7.4], 2 mM MgCl 2 , 1 mM DTT, 1% CHAPS) including protease inhibitor cocktail (Roche). .. The lysate was incubated overnight at 4 C with anti-FLAG beads (Sigma).

Article Title: Interaction of phosphodiesterase 3A with brefeldin A-inhibited guanine nucleotide-exchange proteins BIG1 and BIG2 and effect on ARF1 activity
Article Snippet: .. Briefly, cells (≈5 × 106 ) collected by centrifugation after scraping, were homogenized on ice in 1 mL of 50 mM Tris, pH 7.5, 100 mM NaCl, 2 mM MgCl 2, 0.1% SDS, 0.5% sodium deoxycholate, 1% Triton X-100, 10% glycerol, and Roche protease inhibitor mixture. .. After centrifugation (10,000 × g , 10 min), supernatants were incubated (1.5 h, 4 °C) with 40 μL of CL4B Sepharose beads (50% slurry, Amersham).

Article Title: RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana
Article Snippet: .. Total protein was extracted with the immunoprecipitation (IP) buffer containing 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 4 mM MgCl 2, 5 mM DTT, 0.1% Triton-100, and the complete protease inhibitor cocktail (Roche). .. Cleared protein extracts were immunoprecipitated with agarose-conjugated antibodies against Flag (Sigma M8823) or Myc (Sigma A7470) as described ( ).

Purification:

Article Title: High prevalence of Trypanosoma vegrandis in bats from Western Australia.
Article Snippet: .. PCR amplification with the T. vegrandis-specific primers was performed with the final mix containing 1 l of first round gel purified PCR product, 0.8 M of each primer, 0.02 U/l Kapa Taq (Kapa Biosystems, Massachusetts, U.S.A.), a final concentration of 2.5 mM of each dNTP and 2.5 l of 10× buffer (Kapa Biosystems, Massachusetts, U.S.A.) with 1.5 mM MgCl 2 (Kapa Biosystems, Massachusetts, U.S.A.) and Baxter Ultra-Pure H2O to a volume of 25 l. Cycling conditions consisted of an initial PCR round of 94 • C for 5 min, 50 • C for 2 min and 72 • C for 4 min followed by 45 cycles of 94 • C for 30 s, 60 • C for 30 s and 72 • C for 30 s. The PCR was completed with a final extension of 7 min at 72 • C. .. 18S rRNA amplification and sequencing For amplification of Trypanosoma spp. external forward primer S-LF and external reverse primer S-762 was used in the primary PCR while two pairs of internal primers S-825 and S-LIR (producing a ∼959 bp) and S-823 and S-662 (producing a ∼900 bp) were used in secondary reactions as previously described by Maslov et al. (1996) and McInnes et al. (2009) .

Protein Purification:

Article Title: A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
Article Snippet: .. Generation of RIG-I Mutants Protein Purification and Analysis (His 6 )-Flag-tagged RIG-I(WT) and RIG-I(H830A) were transiently overexpressed in HEK293 blue cells and lysed in a CHAPS-containing lysis buffer (150 mM NaCl, 50 mM Tris/HCl [pH 7.4], 2 mM MgCl 2 , 1 mM DTT, 1% CHAPS) including protease inhibitor cocktail (Roche). .. The lysate was incubated overnight at 4 C with anti-FLAG beads (Sigma).

Immunoprecipitation:

Article Title: RISC-interacting clearing 3’- 5’ exoribonucleases (RICEs) degrade uridylated cleavage fragments to maintain functional RISC in Arabidopsis thaliana
Article Snippet: .. Total protein was extracted with the immunoprecipitation (IP) buffer containing 50 mM Tris-HCl, pH 7.5, 300 mM NaCl, 4 mM MgCl 2, 5 mM DTT, 0.1% Triton-100, and the complete protease inhibitor cocktail (Roche). .. Cleared protein extracts were immunoprecipitated with agarose-conjugated antibodies against Flag (Sigma M8823) or Myc (Sigma A7470) as described ( ).

Incubation:

Article Title: Transepithelial transport of HIV-1 by M cells is receptor-mediated
Article Snippet: .. CD4+ T, monocyte read-out cells were recovered on day 7 postinfection and DNA was extracted from these cells and from Caco-2 or M cell monolayers by an overnight incubation at 57°C with 1.5 mM MgCl 2 , 0.5% Tween 20, 0.5% Nonidet P-40, 12.5 μg of Proteinase K (Roche Molecular Biochemicals). .. DNA was amplified by PCR with SK38 and SK39 gag-specific primers ( ).

Polymerase Chain Reaction:

Article Title: High prevalence of Trypanosoma vegrandis in bats from Western Australia.
Article Snippet: .. PCR amplification with the T. vegrandis-specific primers was performed with the final mix containing 1 l of first round gel purified PCR product, 0.8 M of each primer, 0.02 U/l Kapa Taq (Kapa Biosystems, Massachusetts, U.S.A.), a final concentration of 2.5 mM of each dNTP and 2.5 l of 10× buffer (Kapa Biosystems, Massachusetts, U.S.A.) with 1.5 mM MgCl 2 (Kapa Biosystems, Massachusetts, U.S.A.) and Baxter Ultra-Pure H2O to a volume of 25 l. Cycling conditions consisted of an initial PCR round of 94 • C for 5 min, 50 • C for 2 min and 72 • C for 4 min followed by 45 cycles of 94 • C for 30 s, 60 • C for 30 s and 72 • C for 30 s. The PCR was completed with a final extension of 7 min at 72 • C. .. 18S rRNA amplification and sequencing For amplification of Trypanosoma spp. external forward primer S-LF and external reverse primer S-762 was used in the primary PCR while two pairs of internal primers S-825 and S-LIR (producing a ∼959 bp) and S-823 and S-662 (producing a ∼900 bp) were used in secondary reactions as previously described by Maslov et al. (1996) and McInnes et al. (2009) .

Article Title: Molecular confirmation of an adenovirus in brushtail possums (Trichosurus vulpecula).
Article Snippet: .. For the degenerate primer PCR, virus resuspended in PBS or viral DNA extracted by Dynabeads ® (1.0 ml) was added to the PCR reaction mix containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 1.5 mM MgCl 2 , 100 mM each dNTP, 0.5 U Taq DNA polymerase (Roche) and 1.0 mM of the degenerate primers in a final volume of 12.5 ml. .. Following an initial incubation at 94°C for 1.5 min, PCR was performed for 30 cycles at 94°C 5 s, 50°C 5 s, and 72°C 30 s. For the PoAdV-1 primer-specific long PCR, virus resus-pended in PBS (1.0 ml) was added to the PCR reaction mix containing 20 mM Tris-HCl pH 8.0, 10 mM KCl, 6 mM (NH 4 ) 2 SO 4 , 2 mM MgCl 2 , 0.1% Triton X-100, 10 mg/ml nuclease-free BSA, 100 mM each dNTP, 0.5 U native Pfu DNA polymerase (Stratagene), and 0.2 mM PoAdV E2B F (5%-GTC TAT AAA AGG AAC GTC ATC AG-3%) and PoAdV penton R (5%-CTT TTC TGT TAC TTG TTT ACT CAC-3%) primers in a final volume of 12.5 ml.

Article Title: Amplification refractory mutation system PCR assays for the detection of variola and Orthopoxvirus.
Article Snippet: .. All PCR assays used 320 nmol of dNTPs (Roche), 3 mM MgCl 2 and 0.05 U/l Taq DNA polymerase (Roche). .. Variola virus PCR assays contained between 0.3 and 2.8 ng of infected cell culture DNA per reaction.

Lysis:

Article Title: A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
Article Snippet: .. Generation of RIG-I Mutants Protein Purification and Analysis (His 6 )-Flag-tagged RIG-I(WT) and RIG-I(H830A) were transiently overexpressed in HEK293 blue cells and lysed in a CHAPS-containing lysis buffer (150 mM NaCl, 50 mM Tris/HCl [pH 7.4], 2 mM MgCl 2 , 1 mM DTT, 1% CHAPS) including protease inhibitor cocktail (Roche). .. The lysate was incubated overnight at 4 C with anti-FLAG beads (Sigma).

Reverse Transcription Polymerase Chain Reaction:

Article Title: A simple method for preparing synthetic controls for conventional and real-time PCR for the identification of endemic and exotic disease agents.
Article Snippet: .. Amplification was performed in a Perkin-Elmer, Applied Biosystems 9700 thermocycler using 50 L reactions comprising 1× RT-PCR reaction buffer containing 1.5 mM MgCl 2 , 200 M dNTPs, 10 mM DTT, 20 U of RNasin, 0.2 M of each primer and 1 L of Titan enzyme mix (Roche). ..

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    Roche lysis buffer
    Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 10808 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lysis buffer/product/Roche
    Average 99 stars, based on 10808 article reviews
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    lysis buffer - by Bioz Stars, 2020-07
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    Roche mgcl 2
    Mgcl 2, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl 2/product/Roche
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mgcl 2 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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