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Promega mgcl 2
Mgcl 2, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl 2/product/Promega
Average 91 stars, based on 18 article reviews
Price from $9.99 to $1999.99
mgcl 2 - by Bioz Stars, 2020-07
91/100 stars

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Polymerase Chain Reaction:

Article Title: Characterisation of the sarcomeric myosin heavy chain multigene family in the laboratory guinea pig
Article Snippet: .. Polymerase Chain Reaction (PCR) End-point PCR reactions were performed on 5 μl cDNA in AmpliTaq Gold Buffer (ABI), 1.5 mM MgCl 2 , 0.2 mM dNTP mix (Promega), 0.25 mM forward and reverse oligonucleotide primers (Eurofins) and 1.25 U AmpliTaq (ABI) in a total reaction volume of 50 μl. ..

Article Title: Multicausal etiology of the enteric syndrome in rabbits from Mexico.
Article Snippet: .. The following reagents were added to 1 mg of DNA to make 25 l PCR solution: 2.5 mM MgCl 2 , 100 mM each dATP, dCTP, dGTP, dTTP, 1.5 U GoTaq Flexi DNA Polymerase (PROMEGA, USA), 5 l 5× Green GoTaq Flexi Buffer, and 0.8 mM of each primer. .. The amplification conditions included 35 cycles of denaturation at 96 • C for 45 s, annealing at 54 • C for 45 s and extension at 72 • C for 60 s. A sequenced sample was included as positive control and DNA obtained from rabbit blood was used as negative control.

Article Title: Genetic diversity in the yellow head nidovirus complex.
Article Snippet: .. In the PCR, 1 μl cDNA (equivalent to 50-150 ng RNA) was amplified in a 25 μl reaction containing 1 x Taq DNA polymerase buffer (10 mM Tris-HCl pH 9.0, 50 mM KCl, 0.1% Triton X-100), 1.5 mM MgCl 2 , 17.5 pmol each of primers 2s38M and 2a43M, 200 μM each dNTP and 1.25 U Taq DNA polymerase (Promega). .. The reaction was incubated at 94°C/1 min followed by 35 cycles of 94°C/30 s, 56°C/30 s, 72°C/90 s and then at 72°C/7 min. A 2 μl aliquot of PCR was added nested PCR (25 μl final) prepared as above but containing primers 2s113 and 2a114.

Article Title: A diagnostic oligonucleotide microarray for simultaneous detection of grapevine viruses.
Article Snippet: .. The RT reaction was for 10 min at 25 • C and 50 min at 37 • C in a total volume of 25 l with 200 U of SuperScript II (Invitrogen), 40 U of RNaseOUT (Invitrogen) and 1.6 mM dNTP mix. three l of the product was used for the PCR in a total volume of 50 l with 0.8 mM dNTP mix, 1.5 mM MgCl 2 , 0.5 M specific viral primers and 5 U of Taq DNA polymerase (Promega Corporation, Madison, WI, USA). .. Selected PCR products were purified and cloned for sequencing purposes in the pGEM-T easy vector (Promega).

Article Title: Distribution of nucleotide variants in the DNA sequence of ERCC1 and XRCC1 genes and the effect of phenotype in patients with gastric cancer
Article Snippet: .. A total of 27 PCR reactions were performed for each gene in each individual: 25 μL PCR reaction mixture; 100 ng of genomic DNA, 2.5 μL of 10× PCR buffer solution (Invitrogen Enhancer Buffer) 2.5 mM MgCl 2, 200 mM each of four dNTPs (Promega, Madison.US), 5 pmol forward and reverse primers each, and 1.0 U Platinum Taq Polymerase. .. PCR amplification was done by applying gradient thermal cycler program in Veriti PCR device.

Concentration Assay:

Article Title: Identification and characterization of RNA duplex unwinding and ATPase activities of an alphatetravirus superfamily 1 helicase.
Article Snippet: .. Preparation of double-stranded oligonucleotides Duplex unwinding assays 50 pmol of protein and 0.1 pmol of dsRNA or dsDNA substrate were added to a mixture containing a final concentration of 20 mM HEPES-KOH (pH 7.0), 2.5 mM MgCl 2 , 2 mM DTT, 50 mM KCl and 5U RNasin (Promega, Madison, WI). .. Reaction was initiated by the addition of 5 mM ATP and incubated at 37 1C for 1 h. To minimize reannealing of the unwound oligonucleotides, a 20-fold molar excess of unlabeled DNA trap, corresponding to release strand, was added at the same time as the reaction terminated.

Amplification:

Article Title: Genetic diversity in the yellow head nidovirus complex.
Article Snippet: .. In the PCR, 1 μl cDNA (equivalent to 50-150 ng RNA) was amplified in a 25 μl reaction containing 1 x Taq DNA polymerase buffer (10 mM Tris-HCl pH 9.0, 50 mM KCl, 0.1% Triton X-100), 1.5 mM MgCl 2 , 17.5 pmol each of primers 2s38M and 2a43M, 200 μM each dNTP and 1.25 U Taq DNA polymerase (Promega). .. The reaction was incubated at 94°C/1 min followed by 35 cycles of 94°C/30 s, 56°C/30 s, 72°C/90 s and then at 72°C/7 min. A 2 μl aliquot of PCR was added nested PCR (25 μl final) prepared as above but containing primers 2s113 and 2a114.

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    Promega mgcl 2
    Mgcl 2, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl 2/product/Promega
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    mgcl 2 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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