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PerkinElmer mgcl 2
Mgcl 2, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mgcl 2/product/PerkinElmer
Average 91 stars, based on 13 article reviews
Price from $9.99 to $1999.99
mgcl 2 - by Bioz Stars, 2020-07
91/100 stars

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Amplification:

Article Title: High incidence of human bocavirus infection in children in Spain.
Article Snippet: .. A whole of 5 l of total nucleic acid extracted were added to 45 l of reaction mixture containing 60 mM Tris-HCl (pH 8.5), 15 mM (NH 4 ) 2 SO 4 , 2 mM MgCl 2 , 200 M of each dNTP, 2.5 units of Taq polymerase (Amplitaq, Perkin-Elmer Cetus) and 10 pmol of primers HBOV1f (5 -CACAGGAGCMGGAGYCGCAG) and HBOV1r (5 -CCAAGATATYTRTATCCAGG) for HBoV amplification and 10 pmol of primers RTS (5 -GCTTGGGCGTGTCTCAAAATCT) and RTA (5 -GTCGCCACGGTTGATGAGAGCT) for the internal control amplification. .. Amplifications were carried out in a Peltier Thermal Cycler PTC-200 (MJ Research).

Article Title: Strain typing of Mycoplasma cynos isolates from dogs with respiratory disease.
Article Snippet: .. Amplification was performed in a 50-ml total reaction volume containing 100 ng of DNA sample, 10 mM Tris-HCl (pH 9.0), 1.5 mM MgCl 2 , 50 mM KCl, 0.1% Triton X-100, 0.2 mM each deoxynucleoside triphosphate, and 0.5 U of TaqGold (PerkinElmer). .. Cycling conditions included an initial denaturation step at 94 8C for 5 min, followed by 40 cycles of 94 8C for 15 s, 37 8C for 60 s and 72 8C for 90 s. The last cycle included a final elongation at 72 8C for 7 min. PCR products were resolved by electrophoresis on 10 cm 2% agarose gels at 60 mA for 1.5 h, stained with ethidium bromide and visualized under UV illumination.

Purification:

Article Title: A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
Article Snippet: .. In this assay purified HF-RIG-I was incubated with concentrations of biotinylated RNA for 1 hr at 37 C in buffer (50 mM KCl, 5 mM HEPES [pH 7.0], 3 mM MgCl 2 , 0.5 mM DTT, 0.01% Tween, 1% BSA) and subsequently incubated for 30 min at 25 C with HF-RIG-I-binding Nickel Chelate acceptor beads (Perkin-Elmer) and biotin-RNA-binding Streptavidine donor beads (Perkin Elmer). .. Pull-Down of Endogenous RNA by RIG-I-CTD For the affinity purification of endogenous RNA, a RNA pull-down was conducted using the RNA-binding domain CTD.

Polymerase Chain Reaction:

Article Title: Prevalence and genetic pattern of feline coronaviruses in urban cat populations.
Article Snippet: .. The reaction mixtures contained 5 mL of 10 X GeneAmp PCR buffer II (Perkin-Elmer Cetus), 15 pmol of each primer (OFIP1A and OFIP2A in the first reaction), 2.5 mL of each deoxynucleotide triphosphate (10 mM; Pharmacia), 2.5 mM MgCl 2 , AmpliTaq DNA polymerase (1 U; Perkin-Elmer Cetus), and 5 mL of cDNA. .. Two drops of mineral oil (Sigma) were added to prevent evaporation.

Article Title: Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis.
Article Snippet: .. Reverse transcriptase-polymerase chain reaction (RT-PCR) The components for the first round of the PCR were combined together into a master mix adjusted to give reaction conditions: 20 mM Tris±HCl (pH 8.4), 50 mM KCl, 2.0 mM MgCl 2 , 200 mM dNTP mix, 0.1 U ml À1 Taq DNA polymerase (AmpliTaq Gold Perkin Elmer), 2 ml (5% (v/v)) of the cDNA solution and 0.3 mM FCoV primers (Nos. .. DEPC-treated water was added to make a final volume of 50 ml and the PCR was performed in a temperature cycler with a heated lid (Touchdown±Hybaid).

Article Title: Development of a real-time RT-PCR method for detection of porcine rubulavirus (PoRV-LPMV).
Article Snippet: .. The first PCR was performed in a reaction mix containing 1× PCR buffer, 1.5 mM MgCl 2 , 0.2 M dNTP mix, 2.5 U Ampli Taq DNA polymerase (Perkin Elmer Cetus, Norwalk, CA, USA), 0.2 pmol of each external primer and 2 l of P gene plasmid. ..

Incubation:

Article Title: A Conserved Histidine in the RNA Sensor RIG-I Controls Immune Tolerance to N1-2'O-Methylated Self RNA.
Article Snippet: .. In this assay purified HF-RIG-I was incubated with concentrations of biotinylated RNA for 1 hr at 37 C in buffer (50 mM KCl, 5 mM HEPES [pH 7.0], 3 mM MgCl 2 , 0.5 mM DTT, 0.01% Tween, 1% BSA) and subsequently incubated for 30 min at 25 C with HF-RIG-I-binding Nickel Chelate acceptor beads (Perkin-Elmer) and biotin-RNA-binding Streptavidine donor beads (Perkin Elmer). .. Pull-Down of Endogenous RNA by RIG-I-CTD For the affinity purification of endogenous RNA, a RNA pull-down was conducted using the RNA-binding domain CTD.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Detection of feline coronaviruses by culture and reverse transcriptase-polymerase chain reaction of blood samples from healthy cats and cats with clinical feline infectious peritonitis.
Article Snippet: .. Reverse transcriptase-polymerase chain reaction (RT-PCR) The components for the first round of the PCR were combined together into a master mix adjusted to give reaction conditions: 20 mM Tris±HCl (pH 8.4), 50 mM KCl, 2.0 mM MgCl 2 , 200 mM dNTP mix, 0.1 U ml À1 Taq DNA polymerase (AmpliTaq Gold Perkin Elmer), 2 ml (5% (v/v)) of the cDNA solution and 0.3 mM FCoV primers (Nos. .. DEPC-treated water was added to make a final volume of 50 ml and the PCR was performed in a temperature cycler with a heated lid (Touchdown±Hybaid).

Plasmid Preparation:

Article Title: Development of a real-time RT-PCR method for detection of porcine rubulavirus (PoRV-LPMV).
Article Snippet: .. The first PCR was performed in a reaction mix containing 1× PCR buffer, 1.5 mM MgCl 2 , 0.2 M dNTP mix, 2.5 U Ampli Taq DNA polymerase (Perkin Elmer Cetus, Norwalk, CA, USA), 0.2 pmol of each external primer and 2 l of P gene plasmid. ..

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    PerkinElmer mgcl
    Mgcl, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl/product/PerkinElmer
    Average 92 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    mgcl - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    PerkinElmer mgcl 2
    Mgcl 2, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mgcl 2/product/PerkinElmer
    Average 91 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mgcl 2 - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

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