mg1655 atcc 700926  (ATCC)

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: Strains and plasmids used in this study.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques:

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: MG1655 was challenged with sufficient octanoic acid to inhibit growth by 23% (10 mM) in MOPS minimal media at pH 7.0, 37°C, 150 rpm. This diagram shows central metabolism and highlights the regulatory effect of regulators with significantly perturbed activity during C8 challenge, as identified by network component analysis. Dashed lines indicate regulatory connections that were proposed in our previous analysis . Mechanisms for changes in transcription factor activity are discussed in the text.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Activity Assay

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: E. coli MG1655 pJTD1 was grown to midlog in minimal media at pH(HCl). Values are the average of 4 biological replicates, with error bars indicating the standard deviation.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Standard Deviation

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: Measurements of the intracellular pH of E. coli MG1655 pJTD1 during C8 challenge while grown in the presence of supplemental arginine and glutamate. The cells were incubated for 3°C in MOPS media to allow utilization of the amino acid-dependent acid resistance systems. All concentrations are 10 mM.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Incubation

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: GABA measurements of MG1655 during log phase growth in MOPS with 2% dextrose at 37°C, 150 rpm. All concentrations are 10 mM. GABA: γ-amino butyric acid.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques:

Journal: PLoS ONE

Article Title: Transcriptomic Analysis of Carboxylic Acid Challenge in Escherichia coli : Beyond Membrane Damage

doi: 10.1371/journal.pone.0089580

Figure Lengend Snippet: a: Membrane lipid profile of MG1655 and strains with altered cfa expression. Strains were incubated with 0–30 mM C8, pH = 7.0. Inset: specific growth rate in the log phase of E. coli with varying cfa expression. C16:0- palmitic acid, C16:1- palmitoleic acid, C17cyc- cyclopropane C17:0, C18:1- vaccenic acid, C18:0- stearic acid, C19cyc- cyclopropane C19:0. The complete lipid profiles are shown in of the Supporting Information. Membrane properties are calculated from a to obtain: b: saturated:unsaturated lipid ratio and c: average lipid length.

Article Snippet: MG1655 ATCC#700926 , F- lambda- ilvG- rfb-50 rph-1 , Wildtype.

Techniques: Expressing, Incubation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Unraveling the Gentamicin Drug Product Complexity Reveals Variation in Microbiological Activities and Nephrotoxicity

doi: 10.1128/AAC.00533-20

Figure Lengend Snippet: Microbiological activities of gentamicin congeners against Gram-negative wild-type strains and an isogenic E. coli panel that harbored key aminoglycoside-modifying enzymes

Article Snippet: However, gentamicin C1a had an MIC that was 4-fold more potent than that of the gentamicin mixture, which was significantly different. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Panel Geometric mean MIC value (mg/liter) a Mix b C1a C2 C2a C1 Wild type E. coli ATCC 25922 0.5 0.5 0.5 0.5 0.5 P. aeruginosa ATCC 27853 0.5 0.25 0.25 0.25 1 A. baumannii M2 c 1 0.25 0.5 0.5 2 Isogenic e E. coli ATCC 700926 + empty vector d 0.25 0.125 0.125 0.125 0.125 E. coli ATCC 700926 + aac(6′)-Ib 1 4 0.5 32 0.25 E. coli ATCC 700926 + aac(3)-III 4 2 4 4 32 E. coli ATCC 700926 + aph(3′)-Ia 0.5 0.125 0.25 0.25 0.5 Open in a separate window a MIC values are the geometric mean of multiple independent measurements rounded to the nearest CLSI standard dilution increment. b “Mix” refers to batch of laboratory-grade gentamicin used to purify the gentamicin congeners. c Wild-type A. baumannii M2 has been previously characterized ( 34 ). d Parent E. coli strain containing the empty version of the pBBR1MCS-4 vector ( 35 ) used to carry the aminoglycoside-modifying enzyme genes in the isogenic panel. e Nine additional isogenic E. coli isolates with either aac(6′)-aph(2′′) , aph(3′)-II , aph(3′)-III , aph(3′)-IV , aph(3′)-V , aph(3′)-VII , aac(2′)-I , aac(3)-I , or aac(3)-X were also tested, and the MICs for each congener fell within ±1 log 2 dilution of the gentamicin “Mix” MIC.

Techniques: Plasmid Preparation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Unraveling the Gentamicin Drug Product Complexity Reveals Variation in Microbiological Activities and Nephrotoxicity

doi: 10.1128/AAC.00533-20

Figure Lengend Snippet: Microbiological activities of laboratory-grade gentamicin compared to artificial mixtures of gentamicin congeners that maximized gentamicin C1 and C2 (artificial mix 1) or minimized gentamicin C1 and C2 (artificial mix 2) within USP allowable ranges

Article Snippet: However, gentamicin C1a had an MIC that was 4-fold more potent than that of the gentamicin mixture, which was significantly different. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Panel Geometric mean MIC value (mg/liter) a Mix b C1a C2 C2a C1 Wild type E. coli ATCC 25922 0.5 0.5 0.5 0.5 0.5 P. aeruginosa ATCC 27853 0.5 0.25 0.25 0.25 1 A. baumannii M2 c 1 0.25 0.5 0.5 2 Isogenic e E. coli ATCC 700926 + empty vector d 0.25 0.125 0.125 0.125 0.125 E. coli ATCC 700926 + aac(6′)-Ib 1 4 0.5 32 0.25 E. coli ATCC 700926 + aac(3)-III 4 2 4 4 32 E. coli ATCC 700926 + aph(3′)-Ia 0.5 0.125 0.25 0.25 0.5 Open in a separate window a MIC values are the geometric mean of multiple independent measurements rounded to the nearest CLSI standard dilution increment. b “Mix” refers to batch of laboratory-grade gentamicin used to purify the gentamicin congeners. c Wild-type A. baumannii M2 has been previously characterized ( 34 ). d Parent E. coli strain containing the empty version of the pBBR1MCS-4 vector ( 35 ) used to carry the aminoglycoside-modifying enzyme genes in the isogenic panel. e Nine additional isogenic E. coli isolates with either aac(6′)-aph(2′′) , aph(3′)-II , aph(3′)-III , aph(3′)-IV , aph(3′)-V , aph(3′)-VII , aac(2′)-I , aac(3)-I , or aac(3)-X were also tested, and the MICs for each congener fell within ±1 log 2 dilution of the gentamicin “Mix” MIC.

Techniques: