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Millipore mg n vial 1 na15 no3
N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 <t>mg-N</t> vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).
Mg N Vial 1 Na15 No3, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mg n vial 1 na15 no3 - by Bioz Stars, 2022-11
94/100 stars

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1) Product Images from "Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1"

Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

Journal: bioRxiv

doi: 10.1101/180950

N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 mg-N vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).
Figure Legend Snippet: N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 mg-N vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).

Techniques Used: Cell Culture

Methylophaga nitratireducenticrescens JAM1 growth with N 2 O or NO 3 − as an electron acceptor Strain JAM1 was cultured with 36.4 mg-N vial -1 N 2 O (A) or 18.2 mg-N vial -1 NO 3 − (B) under anoxic conditions. N 2 O, NO 3 − and NO 2 − concentrations and growth were measured over different time intervals. Control (A): N 2 O injected in noninoculated vials. To minimize oxygen contamination, sampling was performed using a glove bag inflated with nitrogen gas. Data represent mean values ± standard deviation (SD; n=3).
Figure Legend Snippet: Methylophaga nitratireducenticrescens JAM1 growth with N 2 O or NO 3 − as an electron acceptor Strain JAM1 was cultured with 36.4 mg-N vial -1 N 2 O (A) or 18.2 mg-N vial -1 NO 3 − (B) under anoxic conditions. N 2 O, NO 3 − and NO 2 − concentrations and growth were measured over different time intervals. Control (A): N 2 O injected in noninoculated vials. To minimize oxygen contamination, sampling was performed using a glove bag inflated with nitrogen gas. Data represent mean values ± standard deviation (SD; n=3).

Techniques Used: Cell Culture, Injection, Sampling, Standard Deviation

N 2 O production by Methylophaga nitratiredu-centicrescens JAM1 Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ). NO 3 − , NO 2 − , N 2 O and N2 (panel A only) concentrations were measured over different time intervals. Data represent mean values ± SD (n=3).
Figure Legend Snippet: N 2 O production by Methylophaga nitratiredu-centicrescens JAM1 Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ). NO 3 − , NO 2 − , N 2 O and N2 (panel A only) concentrations were measured over different time intervals. Data represent mean values ± SD (n=3).

Techniques Used: Cell Culture

Influence of ammonium on NO3 − and NO2 − consumption, and N 2 O production and consumption by Methylophaga nitratireducenticrescens JAM1. Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ) in NH4Cl-free 1403 medium. NO 3 − , NO 2 − and N 2 O concentrations were measured over different time intervals. The results are derived from triplicate cultures. In panel A, asterisks denote the sampling times used for RNA extraction (see Figure 6 ). Data represent mean values ± SD (n=3).
Figure Legend Snippet: Influence of ammonium on NO3 − and NO2 − consumption, and N 2 O production and consumption by Methylophaga nitratireducenticrescens JAM1. Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ) in NH4Cl-free 1403 medium. NO 3 − , NO 2 − and N 2 O concentrations were measured over different time intervals. The results are derived from triplicate cultures. In panel A, asterisks denote the sampling times used for RNA extraction (see Figure 6 ). Data represent mean values ± SD (n=3).

Techniques Used: Cell Culture, Derivative Assay, Sampling, RNA Extraction

Relative transcript levels of cnorB1, cnorB2, nnrS and nosZ . Strain JAM1 was cultured under anoxic conditions in NH4Cl-free 1403 medium with 22 mg-N vial -1 NO 3 − . Growth patterns were similar to those shown in Figure 1B under the same conditions with regular 1403 medium. Samples were drawn from the pre-culture and during the growth phase (no N 2 O production), N 2 O-production phase, and N 2 O-consumption phase (see Figure 4D ), from which total RNA was extracted. Gene expression levels of cnorB1, cnorB2, nnrS and nosZ were measured by RT-qPCR and were reported as the gene copy number per copy of dnaG (reference gene). Student's t -tests were performed for each phase to draw comparisons to the pre-culture phase. *: 0.05
Figure Legend Snippet: Relative transcript levels of cnorB1, cnorB2, nnrS and nosZ . Strain JAM1 was cultured under anoxic conditions in NH4Cl-free 1403 medium with 22 mg-N vial -1 NO 3 − . Growth patterns were similar to those shown in Figure 1B under the same conditions with regular 1403 medium. Samples were drawn from the pre-culture and during the growth phase (no N 2 O production), N 2 O-production phase, and N 2 O-consumption phase (see Figure 4D ), from which total RNA was extracted. Gene expression levels of cnorB1, cnorB2, nnrS and nosZ were measured by RT-qPCR and were reported as the gene copy number per copy of dnaG (reference gene). Student's t -tests were performed for each phase to draw comparisons to the pre-culture phase. *: 0.05

Techniques Used: Cell Culture, Expressing, Quantitative RT-PCR

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    Millipore mg n vial 1 na15 no3
    N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 <t>mg-N</t> vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).
    Mg N Vial 1 Na15 No3, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mg n vial 1 na15 no3/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mg n vial 1 na15 no3 - by Bioz Stars, 2022-11
    94/100 stars
      Buy from Supplier

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    N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 mg-N vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).

    Journal: bioRxiv

    Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

    doi: 10.1101/180950

    Figure Lengend Snippet: N 2 O consumption by Methylophaga nitratireducenticrescens JAM1 under oxic conditions Strain JAM1 was cultured with 3.5 mg-N vial -1 N 2 O or 22 mg-N vial -1 NO 3 − under oxic conditions. N 2 O and growth were measured over different time intervals. Data represent mean values ± SD (n=3).

    Article Snippet: 15 N-labeling of N2 O Strain JAM1 cultures were made with 22 mg-N vial-1 Na15 NO3 (Sigma-Aldrich) in NH4Cl-free medium or with 22 mg-N vial-1 Na14 NO3 and 20.7 mg-N vial-1 15 NH4Cl (Sigma-Aldrich).

    Techniques: Cell Culture

    Methylophaga nitratireducenticrescens JAM1 growth with N 2 O or NO 3 − as an electron acceptor Strain JAM1 was cultured with 36.4 mg-N vial -1 N 2 O (A) or 18.2 mg-N vial -1 NO 3 − (B) under anoxic conditions. N 2 O, NO 3 − and NO 2 − concentrations and growth were measured over different time intervals. Control (A): N 2 O injected in noninoculated vials. To minimize oxygen contamination, sampling was performed using a glove bag inflated with nitrogen gas. Data represent mean values ± standard deviation (SD; n=3).

    Journal: bioRxiv

    Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

    doi: 10.1101/180950

    Figure Lengend Snippet: Methylophaga nitratireducenticrescens JAM1 growth with N 2 O or NO 3 − as an electron acceptor Strain JAM1 was cultured with 36.4 mg-N vial -1 N 2 O (A) or 18.2 mg-N vial -1 NO 3 − (B) under anoxic conditions. N 2 O, NO 3 − and NO 2 − concentrations and growth were measured over different time intervals. Control (A): N 2 O injected in noninoculated vials. To minimize oxygen contamination, sampling was performed using a glove bag inflated with nitrogen gas. Data represent mean values ± standard deviation (SD; n=3).

    Article Snippet: 15 N-labeling of N2 O Strain JAM1 cultures were made with 22 mg-N vial-1 Na15 NO3 (Sigma-Aldrich) in NH4Cl-free medium or with 22 mg-N vial-1 Na14 NO3 and 20.7 mg-N vial-1 15 NH4Cl (Sigma-Aldrich).

    Techniques: Cell Culture, Injection, Sampling, Standard Deviation

    N 2 O production by Methylophaga nitratiredu-centicrescens JAM1 Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ). NO 3 − , NO 2 − , N 2 O and N2 (panel A only) concentrations were measured over different time intervals. Data represent mean values ± SD (n=3).

    Journal: bioRxiv

    Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

    doi: 10.1101/180950

    Figure Lengend Snippet: N 2 O production by Methylophaga nitratiredu-centicrescens JAM1 Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ). NO 3 − , NO 2 − , N 2 O and N2 (panel A only) concentrations were measured over different time intervals. Data represent mean values ± SD (n=3).

    Article Snippet: 15 N-labeling of N2 O Strain JAM1 cultures were made with 22 mg-N vial-1 Na15 NO3 (Sigma-Aldrich) in NH4Cl-free medium or with 22 mg-N vial-1 Na14 NO3 and 20.7 mg-N vial-1 15 NH4Cl (Sigma-Aldrich).

    Techniques: Cell Culture

    Influence of ammonium on NO3 − and NO2 − consumption, and N 2 O production and consumption by Methylophaga nitratireducenticrescens JAM1. Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ) in NH4Cl-free 1403 medium. NO 3 − , NO 2 − and N 2 O concentrations were measured over different time intervals. The results are derived from triplicate cultures. In panel A, asterisks denote the sampling times used for RNA extraction (see Figure 6 ). Data represent mean values ± SD (n=3).

    Journal: bioRxiv

    Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

    doi: 10.1101/180950

    Figure Lengend Snippet: Influence of ammonium on NO3 − and NO2 − consumption, and N 2 O production and consumption by Methylophaga nitratireducenticrescens JAM1. Strain JAM1 was cultured under anoxic (A) or oxic (B) conditions with NO 3 − (22 mg-N vial -1 ) in NH4Cl-free 1403 medium. NO 3 − , NO 2 − and N 2 O concentrations were measured over different time intervals. The results are derived from triplicate cultures. In panel A, asterisks denote the sampling times used for RNA extraction (see Figure 6 ). Data represent mean values ± SD (n=3).

    Article Snippet: 15 N-labeling of N2 O Strain JAM1 cultures were made with 22 mg-N vial-1 Na15 NO3 (Sigma-Aldrich) in NH4Cl-free medium or with 22 mg-N vial-1 Na14 NO3 and 20.7 mg-N vial-1 15 NH4Cl (Sigma-Aldrich).

    Techniques: Cell Culture, Derivative Assay, Sampling, RNA Extraction

    Relative transcript levels of cnorB1, cnorB2, nnrS and nosZ . Strain JAM1 was cultured under anoxic conditions in NH4Cl-free 1403 medium with 22 mg-N vial -1 NO 3 − . Growth patterns were similar to those shown in Figure 1B under the same conditions with regular 1403 medium. Samples were drawn from the pre-culture and during the growth phase (no N 2 O production), N 2 O-production phase, and N 2 O-consumption phase (see Figure 4D ), from which total RNA was extracted. Gene expression levels of cnorB1, cnorB2, nnrS and nosZ were measured by RT-qPCR and were reported as the gene copy number per copy of dnaG (reference gene). Student's t -tests were performed for each phase to draw comparisons to the pre-culture phase. *: 0.05

    Journal: bioRxiv

    Article Title: Denitrifying metabolism of the methylotrophic marine bacterium Methylophaga nitratireducenticrescens strain JAM1

    doi: 10.1101/180950

    Figure Lengend Snippet: Relative transcript levels of cnorB1, cnorB2, nnrS and nosZ . Strain JAM1 was cultured under anoxic conditions in NH4Cl-free 1403 medium with 22 mg-N vial -1 NO 3 − . Growth patterns were similar to those shown in Figure 1B under the same conditions with regular 1403 medium. Samples were drawn from the pre-culture and during the growth phase (no N 2 O production), N 2 O-production phase, and N 2 O-consumption phase (see Figure 4D ), from which total RNA was extracted. Gene expression levels of cnorB1, cnorB2, nnrS and nosZ were measured by RT-qPCR and were reported as the gene copy number per copy of dnaG (reference gene). Student's t -tests were performed for each phase to draw comparisons to the pre-culture phase. *: 0.05

    Article Snippet: 15 N-labeling of N2 O Strain JAM1 cultures were made with 22 mg-N vial-1 Na15 NO3 (Sigma-Aldrich) in NH4Cl-free medium or with 22 mg-N vial-1 Na14 NO3 and 20.7 mg-N vial-1 15 NH4Cl (Sigma-Aldrich).

    Techniques: Cell Culture, Expressing, Quantitative RT-PCR