Structured Review

GenScript mfei
Mfei, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mfei/product/GenScript
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
mfei - by Bioz Stars, 2020-01
92/100 stars

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Mutagenesis:

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments ( GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit ( New England Biolabs ), creating pEL558-pEL561.

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561.

Construct:

Article Title:
Article Snippet: For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments ( GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit ( New England Biolabs ), creating pEL558-pEL561. .. The integrity of all DNA constructs was verified by DNA sequencing.

Article Title:
Article Snippet: For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561. .. The integrity of all DNA constructs was verified by DNA sequencing.

Generated:

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments ( GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit ( New England Biolabs ), creating pEL558-pEL561.

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561.

DNA Sequencing:

Article Title:
Article Snippet: For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments ( GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit ( New England Biolabs ), creating pEL558-pEL561. .. The integrity of all DNA constructs was verified by DNA sequencing.

Article Title:
Article Snippet: For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561. .. The integrity of all DNA constructs was verified by DNA sequencing.

Plasmid Preparation:

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments ( GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit ( New England Biolabs ), creating pEL558-pEL561.

Article Title:
Article Snippet: The CEN ARS HIS3 V5 HTA1(N39C)-HTB1 plasmid (pEL440), was generated by site-directed mutagenesis of pEL305 template. .. For the other V5 HTA1(Cys)-HTB1 plasmids, pEL305 was linearized with BstAPI and MfeI, and synthetic double-stranded DNA fragments (GenScript ) containing individual cysteine substitution at L1 with at least 60 bp of overlapping regions on both sides were recombined using the Gibson assembly kit (New England Biolabs ), creating pEL558-pEL561.

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    GenScript mfei
    Mfei, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mfei/product/GenScript
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mfei - by Bioz Stars, 2020-01
    92/100 stars
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