mfe 296  (Millipore)


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  • 92
    Name:
    MFE 296
    Description:

    Catalog Number:
    cb_98031101
    Price:
    None
    Applications:
    Tumour biology studies, receptor analysis
    Buy from Supplier


    Structured Review

    Millipore mfe 296
    MFE 296

    https://www.bioz.com/result/mfe 296/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mfe 296 - by Bioz Stars, 2020-09
    92/100 stars

    Images

    1) Product Images from "Antitumor effects and molecular mechanisms of ponatinib on endometrial cancer cells harboring activating FGFR2 mutations"

    Article Title: Antitumor effects and molecular mechanisms of ponatinib on endometrial cancer cells harboring activating FGFR2 mutations

    Journal: Cancer Biology & Therapy

    doi: 10.1080/15384047.2015.1108492

    AP24534 inhibits the cell proliferation and FGFR2 kinase activity in endometrial cancer cells with activating FGFR2 mutations. (A) Cells were treated with indicated concentration of AP24534 for 48 h, and the cell viability was determined by the MTT reduction assay. We determined the value of IC 50 of each compound. The concentration of chemicals producing 50% inhibition of cell growth (IC 50 ) was estimated from semilog concentration-inhibition curves constructed using Prism software (GraphPad Software Inc..) (B) MFE-296 and AN3CA cells were treated with 0.1 μM of AP24534 for 48 h. The DNA content (propidium iodide) and cell cycle distribution was analyzed by flow cytometry as described in Material and Methods.
    Figure Legend Snippet: AP24534 inhibits the cell proliferation and FGFR2 kinase activity in endometrial cancer cells with activating FGFR2 mutations. (A) Cells were treated with indicated concentration of AP24534 for 48 h, and the cell viability was determined by the MTT reduction assay. We determined the value of IC 50 of each compound. The concentration of chemicals producing 50% inhibition of cell growth (IC 50 ) was estimated from semilog concentration-inhibition curves constructed using Prism software (GraphPad Software Inc..) (B) MFE-296 and AN3CA cells were treated with 0.1 μM of AP24534 for 48 h. The DNA content (propidium iodide) and cell cycle distribution was analyzed by flow cytometry as described in Material and Methods.

    Techniques Used: Activity Assay, Concentration Assay, MTT Assay, Inhibition, Construct, Software, Flow Cytometry, Cytometry

    AP24534 decreases the phosphorylation of ERK and/or Akt in endometrial cancer cells. ( A , C ) MFE-296 cells and MFE280 cells were treated with indicated concentration of AP24534 and PD17307 for 48 h. Expression levels of both phosphorylated and total Akt and ERK1/2 were measured by Western blot analysis. PTEN expression was determined under the same experimental condition. ( B ) AN3CA cells were treated with AP24534 and PD173074 of the indicated concentrations for 48 h, and then activation/expression of Akt and ERK1/2 were examined by performing western blotting. ( D ) MFE-296 and AN3CA cells were treated with AP24534 of the indicated concentrations for 48 h, and then phosphorylation of PLCγ, PKCα, and STAT5 were assessed by immunoblot analysis. ( E ) In addition, phospho-level of PLCγ, and PKCα was analyzed in MFE280 under the same experimental condition.
    Figure Legend Snippet: AP24534 decreases the phosphorylation of ERK and/or Akt in endometrial cancer cells. ( A , C ) MFE-296 cells and MFE280 cells were treated with indicated concentration of AP24534 and PD17307 for 48 h. Expression levels of both phosphorylated and total Akt and ERK1/2 were measured by Western blot analysis. PTEN expression was determined under the same experimental condition. ( B ) AN3CA cells were treated with AP24534 and PD173074 of the indicated concentrations for 48 h, and then activation/expression of Akt and ERK1/2 were examined by performing western blotting. ( D ) MFE-296 and AN3CA cells were treated with AP24534 of the indicated concentrations for 48 h, and then phosphorylation of PLCγ, PKCα, and STAT5 were assessed by immunoblot analysis. ( E ) In addition, phospho-level of PLCγ, and PKCα was analyzed in MFE280 under the same experimental condition.

    Techniques Used: Concentration Assay, Expressing, Western Blot, Activation Assay

    AP24534 inhibits the anchorage-independent growth of endometrial cancer cells. ( A ) MFE-296 cells were treated with AP24534 (0.01 and 0.1 μM) and PD 173074 (0.1 and 1 μM) twice a week for 3 wks in the soft-agar colony formation assay. ( B ) AN3CA cells were treated with AP24534 (0.001 and 0.01 μM) and PD173074 (0.01 and 0.1 μM) as described in Material and Methods. Colonies were counted using a digital microscope (Olympus DP71, USA) Colony counts from different treatment groups were subjected to statistical analysis. *, p
    Figure Legend Snippet: AP24534 inhibits the anchorage-independent growth of endometrial cancer cells. ( A ) MFE-296 cells were treated with AP24534 (0.01 and 0.1 μM) and PD 173074 (0.1 and 1 μM) twice a week for 3 wks in the soft-agar colony formation assay. ( B ) AN3CA cells were treated with AP24534 (0.001 and 0.01 μM) and PD173074 (0.01 and 0.1 μM) as described in Material and Methods. Colonies were counted using a digital microscope (Olympus DP71, USA) Colony counts from different treatment groups were subjected to statistical analysis. *, p

    Techniques Used: Soft Agar Assay, Microscopy

    AP24534 inhibits the migration and invasion of endometrial cancer cells. ( A ) MFE-296 and AN3CA cells were treated with indicated concentration of AP24534 and PD173074. Cell migration was measured by using the Culture-Inserts, and wound closure was monitored by photography at 36 h following treatment with each compound. ( B ) After treatment of MFE-296 and AN3CA cells with AP24534 (1 μM) and PD173074 (1 μM) for 48 h, an in vitro invasion assay was performed using a 24-well Transwell unit with polycarbonate filters having a diameter of 6.5 mm and a pore size of 8.0 μm, and the number of invading cells per field was counted under light microscopy. *, significantly different between the groups compared ( p
    Figure Legend Snippet: AP24534 inhibits the migration and invasion of endometrial cancer cells. ( A ) MFE-296 and AN3CA cells were treated with indicated concentration of AP24534 and PD173074. Cell migration was measured by using the Culture-Inserts, and wound closure was monitored by photography at 36 h following treatment with each compound. ( B ) After treatment of MFE-296 and AN3CA cells with AP24534 (1 μM) and PD173074 (1 μM) for 48 h, an in vitro invasion assay was performed using a 24-well Transwell unit with polycarbonate filters having a diameter of 6.5 mm and a pore size of 8.0 μm, and the number of invading cells per field was counted under light microscopy. *, significantly different between the groups compared ( p

    Techniques Used: Migration, Concentration Assay, In Vitro, Invasion Assay, Light Microscopy

    (A) MFE-296 cells were treated with the indicated concentrations of AP24534 (0.1 and 1 μM) or PD173074 (1 μM). FGFR2 was immunoprecipitated with FGFR2 antibody and FGFR2 kinase assay was performed as describe in Materials Methods section. *, p
    Figure Legend Snippet: (A) MFE-296 cells were treated with the indicated concentrations of AP24534 (0.1 and 1 μM) or PD173074 (1 μM). FGFR2 was immunoprecipitated with FGFR2 antibody and FGFR2 kinase assay was performed as describe in Materials Methods section. *, p

    Techniques Used: Immunoprecipitation, Kinase Assay

    Related Articles

    Modification:

    Article Title: Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer
    Article Snippet: .. These cells and the Ishikawa 3-H-12 (Sigma-Aldrich, 99040201) and MFE-296 (Sigma-Aldrich, 98031101) cells were grown in Dulbecco's modified Eagle's medium (DMEM; Sigma-Aldrich, 12007559) supplemented with 10% fetal bovine serum (FBS; Invitrogen, 10270106), 1 mmol/L HEPES (Sigma-Aldrich, H3375), 1 mmol/L sodium pyruvate (Sigma-Aldrich, P2256), 2 mmol/L L -glutamine (Sigma-Aldrich, C59202), 1% penicillin/streptomycin (Sigma-Aldrich, P4333) at 37°C with saturating humidity and 5% CO2 . .. For retro-orbital metastatic assay MFE-296 cells were transfected with the luciferase reporter pLKO.Luc (kindly provided by Prof Eloi Garí, Institut de Recerca Biomèdica de Lleida, Catalonia) and selected with puromycin (Sigma-Aldrich, P7255), and maintained in regular medium.

    Transfection:

    Article Title: Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer
    Article Snippet: .. For retro-orbital metastatic assay MFE-296 cells were transfected with the luciferase reporter pLKO.Luc (kindly provided by Prof Eloi Garí, Institut de Recerca Biomèdica de Lleida, Catalonia) and selected with puromycin (Sigma-Aldrich, P7255), and maintained in regular medium. ..

    Luciferase:

    Article Title: Autophagy orchestrates adaptive responses to targeted therapy in endometrial cancer
    Article Snippet: .. For retro-orbital metastatic assay MFE-296 cells were transfected with the luciferase reporter pLKO.Luc (kindly provided by Prof Eloi Garí, Institut de Recerca Biomèdica de Lleida, Catalonia) and selected with puromycin (Sigma-Aldrich, P7255), and maintained in regular medium. ..

    other:

    Article Title: The FOXA2 transcription factor is frequently somatically mutated in uterine carcinosarcomas and carcinomas
    Article Snippet: MFE-280 and MFE-296 were purchased from Sigma-Aldrich.

    Cell Culture:

    Article Title: Analytic validation and real-time clinical application of an amplicon-based targeted gene panel for advanced cancer
    Article Snippet: .. MFE-296 cells (Sigma Aldrich) were cultured in minimal essential media (MEM) (Sigma Aldrich) supplemented with 2 mM L-Glutamine (Sigma Aldrich) and 10% Fetal Bovine Serum (Sigma Aldrich). .. HCC827 cells (ATCC CRL-2868) were obtained from ATCC and cultured in RPMI-1640 medium (Life Technologies, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS) (Sigma Aldrich).