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GE Healthcare megabace 1000 sequencer
Megabace 1000 Sequencer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/megabace 1000 sequencer/product/GE Healthcare
Average 97 stars, based on 9 article reviews
Price from $9.99 to $1999.99
megabace 1000 sequencer - by Bioz Stars, 2020-02
97/100 stars

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Related Articles

Clone Assay:

Article Title: Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14
Article Snippet: .. Random clones were sequenced using ET-Dye terminator chemistry, analyzed with an ABI 3700 sequencer (Applied Biosystems, Foster City, USA) and a MegaBACE 1000 sequencer (Amersham Biosciences, Sweden). ..

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: For automated DNA sequencing, extraction of plasmid DNA was performed from sixteen clones in 96-well microplates. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences). .. The computer program EGassembler from the Human Genome Center was used to trim the vector and mitochondrial sequences.

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: A PCR colony assay was performed on the clones using AD-LD 5′ and AD-LD 3′ supplied oligonucleotides for the pGADT7-Rec bait plasmid. .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis.

Article Title: Characterization of C1q in Teleosts
Article Snippet: Paragraph title: Cloning and Sequencing of Zebrafish c1qA, c1qB, and c1qC cDNAs ... All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia).

Article Title: Genome Sequence of an M3 Strain of Streptococcus pyogenes Reveals a Large-Scale Genomic Rearrangement in Invasive Strains and New Insights into Phage Evolution
Article Snippet: .. We constructed a pUC18-based library containing 1–2 kb and 4–5 kb inserts, and sequenced 48,000 clones (12.6-fold coverage) with Big-Dye terminator chemistry and an ABI 3700 sequencer (Applied Biosystems) and with ET-Dye terminator chemistry and a MegaBACE 1000 sequencer (Amersham Biosciences). .. The sequence was assembled using Phred/Phrap/Consed ( ; ; ).

Amplification:

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: .. The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers. ..

Article Title: Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago
Article Snippet: Successfully amplified products were sequenced for both complementary strands using the DYEnamic™ETDye terminator kit (Amersham Biosciences). .. Samples were run on MegaBACE™ 1000 sequencer (Amersham Biosciences) according to the manufacturer’s protocol.

Article Title: Considerations for monitoring population trends of colonial waterbirds using the effective number of breeders and census estimates. Considerations for monitoring population trends of colonial waterbirds using the effective number of breeders and census estimates
Article Snippet: .. Samples were amplified at 13 species‐specific microsatellite loci (Supporting Information Table ) and genotyped in a MegaBACE® 1000 sequencer (GE Healthcare). .. Genotypes were recorded using F ragment P rofiler ® v1.2 (GE Healthcare), checked for evidence of null alleles using M icro ‐C hecker v2.2.3 (van Oosterhout, Hutchinson, Wills, & Shipley, ), and checked for identical multilocus genotypes using C olony v2.0.6.1 (Jones & Wang, ).

Article Title: Sex, horizontal transmission, and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)
Article Snippet: .. PCR amplification and analysis of fragment sizes followed the protocol from using a MegaBACE 1000 Sequencer (GE Healthcare, Munich, Germany) for DNA fragment separation and the MegaBACE Fragment Profiler software for genotyping. .. DNA extraction of C. bombi Nuclear DNA of the parasite C. bombi was extracted using a modified Chelex protocol ( ) by homogenizing the abdomen (samples of 2008) or the gut (samples of 2009) of each individual in 500 and 300 μL aqua dest, respectively.

Article Title: Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.)
Article Snippet: PCR products were pooled with a loading buffer and size standard mix (MegaBACE 10× Running buffer and MegaBACE ET400-R Size Standard, GE Healthcare, formerly Amersham Biosciences) and electrophoresed using a MegaBACE 1000 sequencer (GE Healthcare). .. Scoring results were classified according to their amplification quality level, as outlined in Primmer & Merilä [ ]: 1: 1 or 2 alleles observed in a single individual, with little stuttering observed; 2: 1 or 2 alleles, moderate stutter; 3: 1 or 2 alleles, considerable stutter; 4: multiple bands and/or smear; 5: no amplification.

TA Cloning:

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: PCR products purified by the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Buckinghamshire, UK) were used for cloning into TOPO TA Cloning ® vector (pCR2.1) from Invitrogen Corporation. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Construct:

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: cDNA libraries were constructed from D. yakuba embryonic (0-14 h) and adult (varying post-eclosion times) stages using the Uni-ZAP XR Library Construction Kit (Stratagene) according to the supplier's instructions. .. The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers.

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: A cDNA library of the M. scutellaris fat body was constructed using a SuperScript Plasmid System kit with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen). .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences).

Article Title: Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.)
Article Snippet: PCR protocols were constructed with annealing temperatures and durations of incubations from published recommendations for the source species in mind. .. PCR products were pooled with a loading buffer and size standard mix (MegaBACE 10× Running buffer and MegaBACE ET400-R Size Standard, GE Healthcare, formerly Amersham Biosciences) and electrophoresed using a MegaBACE 1000 sequencer (GE Healthcare).

Article Title: Genome Sequence of an M3 Strain of Streptococcus pyogenes Reveals a Large-Scale Genomic Rearrangement in Invasive Strains and New Insights into Phage Evolution
Article Snippet: .. We constructed a pUC18-based library containing 1–2 kb and 4–5 kb inserts, and sequenced 48,000 clones (12.6-fold coverage) with Big-Dye terminator chemistry and an ABI 3700 sequencer (Applied Biosystems) and with ET-Dye terminator chemistry and a MegaBACE 1000 sequencer (Amersham Biosciences). .. The sequence was assembled using Phred/Phrap/Consed ( ; ; ).

Real-time Polymerase Chain Reaction:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: Genotyping The chosen SNPs were genotyped using the real-time PCR TaqMan assay utilizing two differentially fluorescently labeled probes that permitted the detection of both alleles in a single reaction (Applied Biosystems INC, Foster City, CA, USA). .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare).

Incubation:

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: The reagents were incubated for 30 min at room temperature and used to transform E.coli electrocompetent TOP10 cells accordingly to the manufacturer instructions. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Modification:

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: Total RNA was extracted from 1g of fresh material using a modified guanidine isothiocyanate procedure (Stratagene). mRNA was isolated using the Poly(A) Quick mRNA Isolation Kit (Stratagene) according to the supplier's instructions. .. The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers.

Article Title: Sex, horizontal transmission, and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)
Article Snippet: Bumblebee genotyping Genomic DNA was extracted from a hind leg per individual using a modified Chelex protocol ( ). .. PCR amplification and analysis of fragment sizes followed the protocol from using a MegaBACE 1000 Sequencer (GE Healthcare, Munich, Germany) for DNA fragment separation and the MegaBACE Fragment Profiler software for genotyping.

Transformation Assay:

Article Title: Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.)
Article Snippet: However, all PCR reactions were transformed to 'touchdown' procedures; starting with a relatively high annealing temperature, gradually decreasing it for each cycle and eventually keeping a fixed annealing temperature for a number of cycles towards the end. .. PCR products were pooled with a loading buffer and size standard mix (MegaBACE 10× Running buffer and MegaBACE ET400-R Size Standard, GE Healthcare, formerly Amersham Biosciences) and electrophoresed using a MegaBACE 1000 sequencer (GE Healthcare).

Ligation:

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: Each ligation reaction used 4 μL of the PCR product, equivalent to 400 ng, 1 μL of salt solution (1.2 M NaCl, 0.06 M MgCl2 ) diluted 4x, 1 μL of TOPO vector and sufficient Milli Q water to complete a final volume of 16 μL. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

DNA Sequencing:

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: For automated DNA sequencing, extraction of plasmid DNA was performed from sixteen clones in 96-well microplates. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis. .. Sequence homologies to the genes of interest were performed by searching the GenBank database using the BLAST algorithm [ ].

Sequencing:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare). .. After the run in the MegaBACE sequencer, the electrofluorograms were visualized using Sequencher ™ v 4.1.4 (Gene Codes Corporation, Ann Arbor, USA ).

Article Title: Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14
Article Snippet: Paragraph title: Whole genome sequencing ... Random clones were sequenced using ET-Dye terminator chemistry, analyzed with an ABI 3700 sequencer (Applied Biosystems, Foster City, USA) and a MegaBACE 1000 sequencer (Amersham Biosciences, Sweden).

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: Paragraph title: cDNA Libraries and Sequencing ... The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers.

Article Title: Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago
Article Snippet: Paragraph title: MtDNA sequencing ... Samples were run on MegaBACE™ 1000 sequencer (Amersham Biosciences) according to the manufacturer’s protocol.

Article Title: Nonsynonymous variants in the SMAD6 gene predispose to congenital cardiovascular malformation
Article Snippet: .. Bidirectional sequencing was performed using MegaBACE 1000 sequencer (GE Healthcare). ..

Article Title: SNP discovery by mismatch-targeting of Mu transposition
Article Snippet: .. A MegaBace 1000 sequencer (GE Healthcare) with Big Dye Terminator chemistry was used for sequencing. .. Sequencing ladders were produced using the Sequenase 2.0 sequencing kit (USB).

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare). .. The files obtained in FASTA format corresponding to the clones were used for contig assembly by the Cap3 Sequence Assembly Program hosted at http://doua.prabi.fr/software/cap3 ( ).

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences). .. The computer program EGassembler from the Human Genome Center was used to trim the vector and mitochondrial sequences.

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis. .. Sequence homologies to the genes of interest were performed by searching the GenBank database using the BLAST algorithm [ ].

Article Title: Characterization of C1q in Teleosts
Article Snippet: .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia). .. A search for potential functional motifs was done in the PROSITE database ( ).

Article Title: Genome Sequence of an M3 Strain of Streptococcus pyogenes Reveals a Large-Scale Genomic Rearrangement in Invasive Strains and New Insights into Phage Evolution
Article Snippet: Paragraph title: Genome Sequencing and Annotation ... We constructed a pUC18-based library containing 1–2 kb and 4–5 kb inserts, and sequenced 48,000 clones (12.6-fold coverage) with Big-Dye terminator chemistry and an ABI 3700 sequencer (Applied Biosystems) and with ET-Dye terminator chemistry and a MegaBACE 1000 sequencer (Amersham Biosciences).

Software:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: Assays were performed on an ABI 7900 HT Fast PCR Real Time System (Applied Biosystems, Foster City, CA ), and the genotype assignments were conducted using the TaqMan ™ Genotyper Software , in a 384-well format and using manufacturer's instructions. .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare).

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare). .. Afterwards, the amino acid (aa) sequences obtained were searched against GenBank with blastx and the sequences with the highest value were selected and subjected to multiple sequence alignment using the ClustalX2.1 software ( .

Article Title: Sex, horizontal transmission, and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)
Article Snippet: .. PCR amplification and analysis of fragment sizes followed the protocol from using a MegaBACE 1000 Sequencer (GE Healthcare, Munich, Germany) for DNA fragment separation and the MegaBACE Fragment Profiler software for genotyping. .. DNA extraction of C. bombi Nuclear DNA of the parasite C. bombi was extracted using a modified Chelex protocol ( ) by homogenizing the abdomen (samples of 2008) or the gut (samples of 2009) of each individual in 500 and 300 μL aqua dest, respectively.

DNA Extraction:

Article Title: Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago
Article Snippet: Total DNA was isolated from buccal or blood samples using the POREGENE DNA isolation kit from Gentra Systems (Minneapolis, USA). .. Samples were run on MegaBACE™ 1000 sequencer (Amersham Biosciences) according to the manufacturer’s protocol.

Mutagenesis:

Article Title: Nonsynonymous variants in the SMAD6 gene predispose to congenital cardiovascular malformation
Article Snippet: Binomial probability indicates that if mutations in the screened sequence were present in 2.5% or greater of CVM cases, sequencing this number of probands would have 80% power of detecting at least one such mutation. .. Bidirectional sequencing was performed using MegaBACE 1000 sequencer (GE Healthcare).

Isolation:

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: Plasmids were isolated by applying an alkaline lyses-diatomaceous earth miniprep protocol optimized for 96-well plates. .. The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers.

Article Title: Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago
Article Snippet: Total DNA was isolated from buccal or blood samples using the POREGENE DNA isolation kit from Gentra Systems (Minneapolis, USA). .. Samples were run on MegaBACE™ 1000 sequencer (Amersham Biosciences) according to the manufacturer’s protocol.

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: mRNA was purified using a Micro-FastTrack 2.0 mRNA isolation kit (Invitrogen) and used to synthesize the first strand of cDNA. .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences).

Article Title: Characterization of C1q in Teleosts
Article Snippet: Total RNA was isolated from spleen and head kidney using TRIzol reagent (Invitrogen) and was reverse-transcribed using 3′-full RACE core set (Takara Bio, Inc.) following the manufacturer's instructions. .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia).

Labeling:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: Genotyping The chosen SNPs were genotyped using the real-time PCR TaqMan assay utilizing two differentially fluorescently labeled probes that permitted the detection of both alleles in a single reaction (Applied Biosystems INC, Foster City, CA, USA). .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare).

Purification:

Article Title: SNP discovery by mismatch-targeting of Mu transposition
Article Snippet: The 33 P-labeled MM1141 was purified and annealed with unlabeled MM1138 to generate a radiolabeled Mu end DNA fragment as described ( ). .. A MegaBace 1000 sequencer (GE Healthcare) with Big Dye Terminator chemistry was used for sequencing.

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: PCR products purified by the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare, Buckinghamshire, UK) were used for cloning into TOPO TA Cloning ® vector (pCR2.1) from Invitrogen Corporation. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Article Title: Color Discrimination in the Tufted Capuchin Monkey, Sapajus spp
Article Snippet: DNA was extracted from blood samples using a purification kit (Puregene DNA, Gentra System). .. The PCR products were directly sequenced using the DYEnamic ET Dye Terminator kit on a MegaBACE 1000 sequencer (GE Healthcare).

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: mRNA was purified using a Micro-FastTrack 2.0 mRNA isolation kit (Invitrogen) and used to synthesize the first strand of cDNA. .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences).

Article Title: Characterization of C1q in Teleosts
Article Snippet: .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia). .. A search for potential functional motifs was done in the PROSITE database ( ).

Polymerase Chain Reaction:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare). .. After the run in the MegaBACE sequencer, the electrofluorograms were visualized using Sequencher ™ v 4.1.4 (Gene Codes Corporation, Ann Arbor, USA ).

Article Title: Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14
Article Snippet: Random clones were sequenced using ET-Dye terminator chemistry, analyzed with an ABI 3700 sequencer (Applied Biosystems, Foster City, USA) and a MegaBACE 1000 sequencer (Amersham Biosciences, Sweden). .. Gaps in the sequence were closed either by sequencing through primer-walking the plasmid templates or by direct sequencing of combinatorial PCR products.

Article Title: An Evolutionary Analysis of Orphan Genes in Drosophila
Article Snippet: .. The clone inserts were fully sequenced on a MegaBACE 1000 sequencer (Molecular Dynamics-Amersham) directly from plasmids or from PCR products after amplification with standard T3/T7 primers and internal primers. ..

Article Title: Carriers of mitochondrial DNA macrohaplogroup L3 basal lineages migrated back to Africa from Asia around 70,000 years ago
Article Snippet: The PCR conditions and procedures for sequencing the mtDNA genome were as previously published [ ]. .. Samples were run on MegaBACE™ 1000 sequencer (Amersham Biosciences) according to the manufacturer’s protocol.

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: Each ligation reaction used 4 μL of the PCR product, equivalent to 400 ng, 1 μL of salt solution (1.2 M NaCl, 0.06 M MgCl2 ) diluted 4x, 1 μL of TOPO vector and sufficient Milli Q water to complete a final volume of 16 μL. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Article Title: Sex, horizontal transmission, and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)
Article Snippet: .. PCR amplification and analysis of fragment sizes followed the protocol from using a MegaBACE 1000 Sequencer (GE Healthcare, Munich, Germany) for DNA fragment separation and the MegaBACE Fragment Profiler software for genotyping. .. DNA extraction of C. bombi Nuclear DNA of the parasite C. bombi was extracted using a modified Chelex protocol ( ) by homogenizing the abdomen (samples of 2008) or the gut (samples of 2009) of each individual in 500 and 300 μL aqua dest, respectively.

Article Title: Color Discrimination in the Tufted Capuchin Monkey, Sapajus spp
Article Snippet: .. The PCR products were directly sequenced using the DYEnamic ET Dye Terminator kit on a MegaBACE 1000 sequencer (GE Healthcare). .. The amino acid sequences from exons 3 and 5 were used to estimate the maximum absorption peak of the pigment within a range of values identified by Asenjo et al. for variant opsins containing the same amino acids at those positions.

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis. .. Sequence homologies to the genes of interest were performed by searching the GenBank database using the BLAST algorithm [ ].

Article Title: Characterization of C1q in Teleosts
Article Snippet: .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia). .. A search for potential functional motifs was done in the PROSITE database ( ).

Article Title: Cross-species amplification of 36 cyprinid microsatellite loci in Phoxinus phoxinus (L.) and Scardinius erythrophthalmus (L.)
Article Snippet: .. PCR products were pooled with a loading buffer and size standard mix (MegaBACE 10× Running buffer and MegaBACE ET400-R Size Standard, GE Healthcare, formerly Amersham Biosciences) and electrophoresed using a MegaBACE 1000 sequencer (GE Healthcare). .. Genotypes were scored using Genetic Profiler 1.5 (GE Healthcare).

Article Title: Genome Sequence of an M3 Strain of Streptococcus pyogenes Reveals a Large-Scale Genomic Rearrangement in Invasive Strains and New Insights into Phage Evolution
Article Snippet: We constructed a pUC18-based library containing 1–2 kb and 4–5 kb inserts, and sequenced 48,000 clones (12.6-fold coverage) with Big-Dye terminator chemistry and an ABI 3700 sequencer (Applied Biosystems) and with ET-Dye terminator chemistry and a MegaBACE 1000 sequencer (Amersham Biosciences). .. Gaps in the sequence were filled by direct PCR sequencing, using primers constructed to anneal to each end of neighboring contigs.

Gel Extraction:

Article Title: Characterization of C1q in Teleosts
Article Snippet: .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia). .. A search for potential functional motifs was done in the PROSITE database ( ).

cDNA Library Assay:

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: A cDNA library of the M. scutellaris fat body was constructed using a SuperScript Plasmid System kit with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen). .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences).

Plasmid Preparation:

Article Title: Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14
Article Snippet: Random clones were sequenced using ET-Dye terminator chemistry, analyzed with an ABI 3700 sequencer (Applied Biosystems, Foster City, USA) and a MegaBACE 1000 sequencer (Amersham Biosciences, Sweden). .. Gaps in the sequence were closed either by sequencing through primer-walking the plasmid templates or by direct sequencing of combinatorial PCR products.

Article Title: Functional complementation of Leishmania(Leishmania) amazonensis AP endonuclease gene (lamap) in Escherichia coli mutant strains challenged with DNA damage agents
Article Snippet: About 300 ng of DNA and 2.5 pmol of primers were used in sequencing reactions with the DYEnamic ET Dye Terminator Kit (Amersham Biosciences, Amersham, UK) combined with the F-M13 primers (M13 universal 5’-GTAAAACGACGGCCAGT-3’) and M13 -R (5’-M13 reverse CAGGAAACAGCTATGAC-3‘), allowing the extension from the primers and vector-Lamap2F-Bg and Lamap2R-Bg specific for lamap gene. .. Automated sequencing was performed on a MegaBace 1000 sequencer (GE Healthcare).

Article Title: Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
Article Snippet: A cDNA library of the M. scutellaris fat body was constructed using a SuperScript Plasmid System kit with Gateway Technology for cDNA Synthesis and Cloning (Invitrogen). .. The clones were sequenced using DYEnamic ET Dye Terminator Cycle Sequencing for MegaBace DNA Analysis Systems kits (GE Healthcare) according to the manufacturers recommendations and analyzed with a MegaBace 1000 sequencer (Amersham Biosciences).

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: A PCR colony assay was performed on the clones using AD-LD 5′ and AD-LD 3′ supplied oligonucleotides for the pGADT7-Rec bait plasmid. .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis.

Article Title: Characterization of C1q in Teleosts
Article Snippet: .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia). .. A search for potential functional motifs was done in the PROSITE database ( ).

TaqMan Assay:

Article Title: Association of Genetic Variants with Self-Assessed Color Categories in Brazilians
Article Snippet: .. The classification accuracy of each TaqMan assay was validated by cycle sequencing (forward and reverse) of PCR fragments containing the polymorphisms studied using the DYEnamicTM ET Dye Terminator Kit, (GE Healthcare) standard procedure and a MegaBACE™ 1000 sequencer (GE Healthcare). .. After the run in the MegaBACE sequencer, the electrofluorograms were visualized using Sequencher ™ v 4.1.4 (Gene Codes Corporation, Ann Arbor, USA ).

Multiplex Assay:

Article Title: Sex, horizontal transmission, and multiple hosts prevent local adaptation of Crithidia bombi, a parasite of bumblebees (Bombus spp.)
Article Snippet: The genotyping multiplex PCR primer set (fluorescent-labeled B10, B11, B124, B126, and B100) is based on , . .. PCR amplification and analysis of fragment sizes followed the protocol from using a MegaBACE 1000 Sequencer (GE Healthcare, Munich, Germany) for DNA fragment separation and the MegaBACE Fragment Profiler software for genotyping.

Agarose Gel Electrophoresis:

Article Title: Characterization of C1q in Teleosts
Article Snippet: PCR products were loaded on a 1.2% (w/v) agarose gel and visualized by staining with 0.1 mg/ml ethidium bromide. .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia).

Chromosome Walking:

Article Title: Genome evolution driven by host adaptations results in a more virulent and antimicrobial-resistant Streptococcus pneumoniae serotype 14
Article Snippet: Random clones were sequenced using ET-Dye terminator chemistry, analyzed with an ABI 3700 sequencer (Applied Biosystems, Foster City, USA) and a MegaBACE 1000 sequencer (Amersham Biosciences, Sweden). .. Gaps in the sequence were closed either by sequencing through primer-walking the plasmid templates or by direct sequencing of combinatorial PCR products.

Colony Assay:

Article Title: Intermolecular interactions of the malate synthase of Paracoccidioides spp
Article Snippet: A PCR colony assay was performed on the clones using AD-LD 5′ and AD-LD 3′ supplied oligonucleotides for the pGADT7-Rec bait plasmid. .. The PCR products of the identified transformants were subjected to DNA sequencing using a MegaBACE 1000 sequencer (GE Healthcare®) for automated sequence analysis.

Produced:

Article Title: SNP discovery by mismatch-targeting of Mu transposition
Article Snippet: A MegaBace 1000 sequencer (GE Healthcare) with Big Dye Terminator chemistry was used for sequencing. .. Sequencing ladders were produced using the Sequenase 2.0 sequencing kit (USB).

Staining:

Article Title: Characterization of C1q in Teleosts
Article Snippet: PCR products were loaded on a 1.2% (w/v) agarose gel and visualized by staining with 0.1 mg/ml ethidium bromide. .. All PCR products were purified using a gel extraction kit (Qiagen), cloned into the pUCm-T vector (Takara Bio, Inc.), and then sequenced on MegaBACE 1000 Sequencer (GE Healthcare) using a DYEnamic ET dye terminator cycle sequencing kit (Pharmacia).

Variant Assay:

Article Title: Color Discrimination in the Tufted Capuchin Monkey, Sapajus spp
Article Snippet: The PCR products were directly sequenced using the DYEnamic ET Dye Terminator kit on a MegaBACE 1000 sequencer (GE Healthcare). .. The amino acid sequences from exons 3 and 5 were used to estimate the maximum absorption peak of the pigment within a range of values identified by Asenjo et al. for variant opsins containing the same amino acids at those positions.

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