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MiddleBrook Pharmaceuticals medium
Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 2 article reviews
Price from $9.99 to $1999.99
medium - by Bioz Stars, 2020-04
95/100 stars

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Related Articles

Centrifugation:

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: Following exposure to NALC-NaOH at 23°C and 30°C, the cell pellets were harvested by centrifugation at 3,000 ×  g for 20 min at 4°C. .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ).

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: Following exposure to NALC-NaOH at 23°C and 30°C, the cell pellets were harvested by centrifugation at 3,000 × g for 20 min at 4°C. .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ).

In Vitro:

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ). .. All culture media (7H11 and MGIT) used for the in vitro M. tuberculosis experiments were free from selective antibiotics.

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ). .. All culture media (7H11 and MGIT) used for the in vitro M. tuberculosis experiments were free from selective antibiotics.

Article Title: In Vitro Study of Stepwise Acquisition of rv0678 and atpE Mutations Conferring Bedaquiline Resistance
Article Snippet: Exposure to increasing bedaquiline concentrations resulted in increasing phenotypic resistance (up to > 2 μg/ml) through MIC determination on solid medium (Middlebrook 7H10). rv0678 mutations were dynamic, while atpE mutations were fixed, once occurring. .. We present the following hypothesis for in vitro emergence of bedaquiline resistance: rv0678 mutations may be the first transient step in low-level resistance acquisition, followed by high-level resistance due to fixed atpE mutations.

Modification:

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin). .. Group typing was done using IS 1311 polymerase chain reaction restriction enzyme analysis (PCR-REA) directly from Map colonies.

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin). .. Slants were incubated at 37°C and observed every 2 wk for the appearance of colonies for a maximum of 36 wk.

Concentration Assay:

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: The 2-ml H37Rv culture at a concentration of ∼107 CFU/ml was processed with an equal volume of NALC-NaOH (1% final concentration of NaOH) at 23°C and 30°C for 20 min. For controls, another 2 ml of culture aliquots was treated with an equal volume of phosphate-buffered saline (PBS), pH 6.8, instead of NALC-NaOH ( ). .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ).

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: The 2-ml H37Rv culture at a concentration of ∼107 CFU/ml was processed with an equal volume of NALC-NaOH (1% final concentration of NaOH) at 23°C and 30°C for 20 min. For controls, another 2 ml of culture aliquots was treated with an equal volume of phosphate-buffered saline (PBS), pH 6.8, instead of NALC-NaOH ( ). .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ).

Mutagenesis:

Article Title: In Vitro Study of Stepwise Acquisition of rv0678 and atpE Mutations Conferring Bedaquiline Resistance
Article Snippet: M. tuberculosis mutant populations from each of five sequential steps in a passaging approach, using a pyrazinamide-resistant ATCC strain, were subjected to MIC determinations and whole-genome sequencing. .. Exposure to increasing bedaquiline concentrations resulted in increasing phenotypic resistance (up to > 2 μg/ml) through MIC determination on solid medium (Middlebrook 7H10). rv0678 mutations were dynamic, while atpE mutations were fixed, once occurring.

Article Title: A Riboswitch Regulates Expression of the Coenzyme B12-Independent Methionine Synthase in Mycobacterium tuberculosis: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551 ▿: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551 ▿ †
Article Snippet: We postulated that a mutation in the B12 riboswitch motif upstream of metE might relieve B12 -mediated suppression of metE and so allow growth of these “vitamin B12 suppressor” mutants. .. To ascertain the heritability of the suppressor phenotype, selected colonies were passaged in liquid medium (Middlebrook 7H9; Difco) without B12 supplement.

Isolation:

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: To increase the likelihood of small ruminant Map strain isolation and following discussions with the manufacturer, the tubes were incubated for 3 wk at 35°C and then transferred to the MGIT 960 apparatus for 2 consecutive cycles of 7 wk of incubation. .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: To increase the likelihood of small ruminant Map strain isolation and following discussions with the manufacturer, the tubes were incubated for 3 wk at 35°C and then transferred to the MGIT 960 apparatus for 2 consecutive cycles of 7 wk of incubation. .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Passaging:

Article Title: In Vitro Study of Stepwise Acquisition of rv0678 and atpE Mutations Conferring Bedaquiline Resistance
Article Snippet: M. tuberculosis mutant populations from each of five sequential steps in a passaging approach, using a pyrazinamide-resistant ATCC strain, were subjected to MIC determinations and whole-genome sequencing. .. Exposure to increasing bedaquiline concentrations resulted in increasing phenotypic resistance (up to > 2 μg/ml) through MIC determination on solid medium (Middlebrook 7H10). rv0678 mutations were dynamic, while atpE mutations were fixed, once occurring.

Polymerase Chain Reaction:

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Presumptive positive vials were confirmed by acid fast stain and by PCR ( Taq Man Map Johne’s Reagents; Life Technologies, Mississauga, Ontario). .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Presumptive positive vials were confirmed by acid fast stain and by PCR ( Taq Man Map Johne’s Reagents; Life Technologies, Mississauga, Ontario). .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Incubation:

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ). .. The 7H11 medium was supplemented with OADC (Oxoid, United Kingdom), and the plates were observed weekly for any growth of M. tuberculosis colonies for up to 6 weeks.

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: To increase the likelihood of small ruminant Map strain isolation and following discussions with the manufacturer, the tubes were incubated for 3 wk at 35°C and then transferred to the MGIT 960 apparatus for 2 consecutive cycles of 7 wk of incubation. .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Article Title: Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability
Article Snippet: .. Quadruplicates of each dilution were inoculated on solid medium (Middlebrook 7H11; Becton, Dickinson and Company, MD, USA) using the method of Miles et al. ( ) and incubated at 37°C for determination of colony counts ( ). .. The 7H11 medium was supplemented with OADC (Oxoid, United Kingdom), and the plates were observed weekly for any growth of M. tuberculosis colonies for up to 6 weeks.

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: To increase the likelihood of small ruminant Map strain isolation and following discussions with the manufacturer, the tubes were incubated for 3 wk at 35°C and then transferred to the MGIT 960 apparatus for 2 consecutive cycles of 7 wk of incubation. .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

other:

Article Title: Novel Multipurpose Methodology for Detection of Mycobacteria in Pulmonary and Extrapulmonary Specimens by Smear Microscopy, Culture, and PCR †
Article Snippet: A similar observation was also made with USP-treated bacteria; after USP treatment, colonies appeared on solid medium (Middlebrook 7H10 or LJ) after a lag time of ∼7 to 10 days compared to untreated bacteria.

Sequencing:

Article Title: In Vitro Study of Stepwise Acquisition of rv0678 and atpE Mutations Conferring Bedaquiline Resistance
Article Snippet: M. tuberculosis mutant populations from each of five sequential steps in a passaging approach, using a pyrazinamide-resistant ATCC strain, were subjected to MIC determinations and whole-genome sequencing. .. Exposure to increasing bedaquiline concentrations resulted in increasing phenotypic resistance (up to > 2 μg/ml) through MIC determination on solid medium (Middlebrook 7H10). rv0678 mutations were dynamic, while atpE mutations were fixed, once occurring.

Ziehl-Neelsen Stain:

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Presumptive positive vials were confirmed by acid fast stain and by PCR ( Taq Man Map Johne’s Reagents; Life Technologies, Mississauga, Ontario). .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Article Title: Molecular characterization of Mycobacterium avium subspecies paratuberculosis C-type and S-type isolated from sheep and goats by using a combination of MIRU-VNTR loci
Article Snippet: Presumptive positive vials were confirmed by acid fast stain and by PCR ( Taq Man Map Johne’s Reagents; Life Technologies, Mississauga, Ontario). .. Aliquots of 100 μL of each dilution were then inoculated on 5 replicate slants of modified medium (MiddleBrook 7H10) with mycobactin ( ) and egg yolk medium (Herrold’s Egg Yolk medium; BD with mycobactin).

Derivative Assay:

Article Title: A Riboswitch Regulates Expression of the Coenzyme B12-Independent Methionine Synthase in Mycobacterium tuberculosis: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551 ▿: Implications for Differential Methionine Synthase Function in Strains H37Rv and CDC1551 ▿ †
Article Snippet: To ascertain the heritability of the suppressor phenotype, selected colonies were passaged in liquid medium (Middlebrook 7H9; Difco) without B12 supplement. .. We also sequenced 10 B12 suppressor mutants derived from H37Rv Δ metH (B).

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  • 93
    MiddleBrook Pharmaceuticals 7h10 oadc medium
    Mutations within HadA or HadC confer growth resistance during ISO treatment. Mid-log-phase cultures of wild-type (WT) M. tuberculosis and strains carrying either the C61G mutation in HadA (MTTR3) or the V85F mutation in HadC (MTTR18) were streaked onto Middlebrook <t>7H10</t> <t>OADC</t> plates supplemented with increasing concentrations of ISO (1, 2.5, and 5 μg/ml). Plates were incubated for 2 to 3 weeks at 37°C, after which growth was visualized.
    7h10 Oadc Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/7h10 oadc medium/product/MiddleBrook Pharmaceuticals
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    7h10 oadc medium - by Bioz Stars, 2020-04
    93/100 stars
      Buy from Supplier

    90
    MiddleBrook Pharmaceuticals nacl middlebrook 7h10 medium
    M. bolettii colonies on <t>7H10,</t> 7H10-1 <t>%-NaCl,</t> 7H10-2 %-NaCl and 7H10-3 %-NaCl
    Nacl Middlebrook 7h10 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nacl middlebrook 7h10 medium/product/MiddleBrook Pharmaceuticals
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nacl middlebrook 7h10 medium - by Bioz Stars, 2020-04
    90/100 stars
      Buy from Supplier

    99
    MiddleBrook Pharmaceuticals middlebrook 7h9 medium
    FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in <t>Middlebrook</t> <t>7H9</t> medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.
    Middlebrook 7h9 Medium, supplied by MiddleBrook Pharmaceuticals, used in various techniques. Bioz Stars score: 99/100, based on 188 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/middlebrook 7h9 medium/product/MiddleBrook Pharmaceuticals
    Average 99 stars, based on 188 article reviews
    Price from $9.99 to $1999.99
    middlebrook 7h9 medium - by Bioz Stars, 2020-04
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    Image Search Results


    Mutations within HadA or HadC confer growth resistance during ISO treatment. Mid-log-phase cultures of wild-type (WT) M. tuberculosis and strains carrying either the C61G mutation in HadA (MTTR3) or the V85F mutation in HadC (MTTR18) were streaked onto Middlebrook 7H10 OADC plates supplemented with increasing concentrations of ISO (1, 2.5, and 5 μg/ml). Plates were incubated for 2 to 3 weeks at 37°C, after which growth was visualized.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Point Mutations within the Fatty Acid Synthase Type II Dehydratase Components HadA or HadC Contribute to Isoxyl Resistance in Mycobacterium tuberculosis

    doi: 10.1128/AAC.01972-12

    Figure Lengend Snippet: Mutations within HadA or HadC confer growth resistance during ISO treatment. Mid-log-phase cultures of wild-type (WT) M. tuberculosis and strains carrying either the C61G mutation in HadA (MTTR3) or the V85F mutation in HadC (MTTR18) were streaked onto Middlebrook 7H10 OADC plates supplemented with increasing concentrations of ISO (1, 2.5, and 5 μg/ml). Plates were incubated for 2 to 3 weeks at 37°C, after which growth was visualized.

    Article Snippet: The MICs of ISO and TAC were determined on Middlebrook 7H10 OADC medium containing 20 μg/ml pantothenic acid and increasing drug concentrations.

    Techniques: Mutagenesis, Incubation

    M. bolettii colonies on 7H10, 7H10-1 %-NaCl, 7H10-2 %-NaCl and 7H10-3 %-NaCl

    Journal: BMC Research Notes

    Article Title: Inverse correlation between salt tolerance and host-adaptation in mycobacteria

    doi: 10.1186/s13104-016-2054-y

    Figure Lengend Snippet: M. bolettii colonies on 7H10, 7H10-1 %-NaCl, 7H10-2 %-NaCl and 7H10-3 %-NaCl

    Article Snippet: As for M. bolettii , the size of colonies increased from 1.16 ± 0.4 mm in the Middlebrook 7H10 control medium up to 2.95 ± 0.9 mm in 3 % NaCl Middlebrook 7H10 medium (P < 0.05, student’s t test), then decreased down to 0.4 ± 0.2 mm in 7 % NaCl Middlebrook 7H10 medium (P < 0.05, student’s t test) (Fig. ; Table ).

    Techniques:

    FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in Middlebrook 7H9 medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Mycobacterium avium subsp. paratuberculosis (Map) Fatty Acids Profile Is Strain-Dependent and Changes Upon Host Macrophages Infection

    doi: 10.3389/fcimb.2017.00089

    Figure Lengend Snippet: FAs of the K10 (Type C) and 2349/06-1 (Type S) isolates of Map that showed statistically significant differences in abundance between the two isolates . BOMAC and MOCL-4 cells were infected with the K10 and 2349/06-1 isolates of Map at MOI of 1:10. At 4 h p.i., the medium containing the extracellular bacteria was collected, centrifuged at 2000 g for 15 min and the FAMEs of the bacterial pellets extracted and analyzed by GC. FAs of the K10 and 2349/06-1 isolates of Map grown in Middlebrook 7H9 medium that showed statistically significant differences in abundance are also included in the figure. Relative amount of each FA for each experimental condition (7H9 medium or extracellular) is presented as the percentage of the total FAs content. Statistically significant differences are indicated with an asterisk.

    Article Snippet: The relative FAs composition of the two isolates recovered from infected BOMAC and MOCL-4 cells was determined by gas chromatography and compared with that of extracellular bacteria and that of bacteria grown in Middlebrook 7H9 medium.

    Techniques: Infection

    Live cell imaging of Msm MLP cell undergoing ACD in the presence of rifampicin, its lineage and data on the time taken by the rifampicin-stressed sister daughter cells (post-birth) generated from ACD . (A) Live cell imaging panels of Msm cells exposed to 12.5 μg/ml rifampicin for a period of 50 min. Rifampicin exposure was given for 50 min in agarose pad, t = 3:10-4:00 (red square), replaced with Middlebrook 7H9 medium after t = 4:00 and monitored for 10 h. Cell, marked with blue color, underwent asymmetric division ge nerating a short daughter cell and a normal/long-sized daughter cell. The arrows show the division sites in the respective frames. Each cell is represented in different color and as the cell divided, the daughter cells have been given a different color in the cartoon representation. The panel (A) corresponds to the images in the Video S2 . (B) The lineage of the growth and division of the rifampicin-stressed Msm cell undergoing ACD (shown in the panel A ). (C) Time taken for the onset of the next division of rifampicin-treated sister daughter cells (normal/long-sized cell and short cell) generated from ACD, data shown in (D) . The sister cells have been marked in the same color, in (C) . The * sign in (D) indicates the ACD-generated short cell that never grew in the observed time period.

    Journal: Frontiers in Microbiology

    Article Title: Mycobacterial Cultures Contain Cell Size and Density Specific Sub-populations of Cells with Significant Differential Susceptibility to Antibiotics, Oxidative and Nitrite Stress

    doi: 10.3389/fmicb.2017.00463

    Figure Lengend Snippet: Live cell imaging of Msm MLP cell undergoing ACD in the presence of rifampicin, its lineage and data on the time taken by the rifampicin-stressed sister daughter cells (post-birth) generated from ACD . (A) Live cell imaging panels of Msm cells exposed to 12.5 μg/ml rifampicin for a period of 50 min. Rifampicin exposure was given for 50 min in agarose pad, t = 3:10-4:00 (red square), replaced with Middlebrook 7H9 medium after t = 4:00 and monitored for 10 h. Cell, marked with blue color, underwent asymmetric division ge nerating a short daughter cell and a normal/long-sized daughter cell. The arrows show the division sites in the respective frames. Each cell is represented in different color and as the cell divided, the daughter cells have been given a different color in the cartoon representation. The panel (A) corresponds to the images in the Video S2 . (B) The lineage of the growth and division of the rifampicin-stressed Msm cell undergoing ACD (shown in the panel A ). (C) Time taken for the onset of the next division of rifampicin-treated sister daughter cells (normal/long-sized cell and short cell) generated from ACD, data shown in (D) . The sister cells have been marked in the same color, in (C) . The * sign in (D) indicates the ACD-generated short cell that never grew in the observed time period.

    Article Snippet: Determination of the regeneration potential, heat sensitivity, and viability of SCs and NCs The cells from SCF1 and SCF2 mixture and from NCF were independently inoculated into fresh Middlebrook 7H9 medium as well as plated on Middlebrook 7H10 agar medium, and examined the cells from MLP culture and from colonies from the plates, under microscope (DIC).

    Techniques: Live Cell Imaging, Generated

    Panels from Video S1 on the live cell imaging of Msm short cells generating micro colony and population regeneration potential of the cells in the SCF and NCF . (A) An Msm cell elongates and divides symmetrically to generate two short daughter cells. One of the short daughter cells (green), grew and divided asymmetrically to generate two unequal-sized daughter cells (indicated in cyan and pink). The other short daughter cell (red), grew and divided symmetrically to generate two comparably-sized short daughter cells (indicated in white and yellow). The daughter cells from the earlier asymmetric division (cyan) further divided symmetrically to generate two daughter cells. The short daughter cell (yellow) further grew and divided symmetrically to generate two daughter cells. Arrows show the site of constriction. Cells of length ≤ 2.60 ± 0.25 μm were considered short cells (as per Figure 1B ). (B) Fraction enriched for Msm short cells. (C) Cells from mid-log phase population. (D) Population generated from the short-cells-enriched fraction after reinoculation into Middlebrook 7H9 liquid medium. (E) Population generated from plating of the cells in the short-cells-enriched fraction on Middlebrook 7H10 agar. The compositions of the cells in (D,E) are similar to that in (C) . Arrows indicate short cells. Length of Msm short cells are shown in (B) . (F–I) Acid-fast stained cells in the SCF and NCF and the respective population generated from them after reinoculation into Middlebrook 7H9 media and incubated till the cultures reached 0.6 OD 600nm .

    Journal: Frontiers in Microbiology

    Article Title: Mycobacterial Cultures Contain Cell Size and Density Specific Sub-populations of Cells with Significant Differential Susceptibility to Antibiotics, Oxidative and Nitrite Stress

    doi: 10.3389/fmicb.2017.00463

    Figure Lengend Snippet: Panels from Video S1 on the live cell imaging of Msm short cells generating micro colony and population regeneration potential of the cells in the SCF and NCF . (A) An Msm cell elongates and divides symmetrically to generate two short daughter cells. One of the short daughter cells (green), grew and divided asymmetrically to generate two unequal-sized daughter cells (indicated in cyan and pink). The other short daughter cell (red), grew and divided symmetrically to generate two comparably-sized short daughter cells (indicated in white and yellow). The daughter cells from the earlier asymmetric division (cyan) further divided symmetrically to generate two daughter cells. The short daughter cell (yellow) further grew and divided symmetrically to generate two daughter cells. Arrows show the site of constriction. Cells of length ≤ 2.60 ± 0.25 μm were considered short cells (as per Figure 1B ). (B) Fraction enriched for Msm short cells. (C) Cells from mid-log phase population. (D) Population generated from the short-cells-enriched fraction after reinoculation into Middlebrook 7H9 liquid medium. (E) Population generated from plating of the cells in the short-cells-enriched fraction on Middlebrook 7H10 agar. The compositions of the cells in (D,E) are similar to that in (C) . Arrows indicate short cells. Length of Msm short cells are shown in (B) . (F–I) Acid-fast stained cells in the SCF and NCF and the respective population generated from them after reinoculation into Middlebrook 7H9 media and incubated till the cultures reached 0.6 OD 600nm .

    Article Snippet: Determination of the regeneration potential, heat sensitivity, and viability of SCs and NCs The cells from SCF1 and SCF2 mixture and from NCF were independently inoculated into fresh Middlebrook 7H9 medium as well as plated on Middlebrook 7H10 agar medium, and examined the cells from MLP culture and from colonies from the plates, under microscope (DIC).

    Techniques: Live Cell Imaging, Generated, Staining, Incubation

    Live cell imaging of Msm MLP cells undergoing ACD and SCD in the presence of H 2 O 2 and their respective lineage. (A) Live cell imaging panels of Msm cells exposed to 0.8 mM H 2 O 2 for a period of 1 h, immediately after the first division and was replaced with Middlebrook 7H9 medium after 1 h of exposure. (ACD; the cell on the right side in the panels, SCD; the lower of the two cells on the left side in the panels). H 2 O 2 was introduced at 50th min (indicated by the red square at the lower bottom of the panels) and was replaced with the medium at 1 h 50th min (indicated by the green square at the lower bottom of the panels). The treated cells were observed for about 9 h. The panel (A) corresponds to the images in the Video S3 . (B) The lineage of the growth and division of the H 2 O 2 -stressed Msm cell undergoing ACD (shown in the right side of the panel A ). (C) The lineage of the growth and division of the H 2 O 2 -stressed Msm cell undergoing SCD (shown in the left side of the panel A ). The zero time point does not correlate with the birth of the starting mother cell. The cell lengths given are from the DIC images, but not drawn to scale. The time of generation of daughter cells from asymmetric cell division (ACD) and symmetric cell division (SCD) has been indicated for each division. The time point of exposure to the stress and its withdrawal have been indicated with red arrows.

    Journal: Frontiers in Microbiology

    Article Title: Mycobacterial Cultures Contain Cell Size and Density Specific Sub-populations of Cells with Significant Differential Susceptibility to Antibiotics, Oxidative and Nitrite Stress

    doi: 10.3389/fmicb.2017.00463

    Figure Lengend Snippet: Live cell imaging of Msm MLP cells undergoing ACD and SCD in the presence of H 2 O 2 and their respective lineage. (A) Live cell imaging panels of Msm cells exposed to 0.8 mM H 2 O 2 for a period of 1 h, immediately after the first division and was replaced with Middlebrook 7H9 medium after 1 h of exposure. (ACD; the cell on the right side in the panels, SCD; the lower of the two cells on the left side in the panels). H 2 O 2 was introduced at 50th min (indicated by the red square at the lower bottom of the panels) and was replaced with the medium at 1 h 50th min (indicated by the green square at the lower bottom of the panels). The treated cells were observed for about 9 h. The panel (A) corresponds to the images in the Video S3 . (B) The lineage of the growth and division of the H 2 O 2 -stressed Msm cell undergoing ACD (shown in the right side of the panel A ). (C) The lineage of the growth and division of the H 2 O 2 -stressed Msm cell undergoing SCD (shown in the left side of the panel A ). The zero time point does not correlate with the birth of the starting mother cell. The cell lengths given are from the DIC images, but not drawn to scale. The time of generation of daughter cells from asymmetric cell division (ACD) and symmetric cell division (SCD) has been indicated for each division. The time point of exposure to the stress and its withdrawal have been indicated with red arrows.

    Article Snippet: Determination of the regeneration potential, heat sensitivity, and viability of SCs and NCs The cells from SCF1 and SCF2 mixture and from NCF were independently inoculated into fresh Middlebrook 7H9 medium as well as plated on Middlebrook 7H10 agar medium, and examined the cells from MLP culture and from colonies from the plates, under microscope (DIC).

    Techniques: Live Cell Imaging