Journal: Cell Biology and Toxicology

Article Title: SENP3 mediated DeSUMOylation of macrophage derived CCL17 accelerates atherosclerosis via regulation of Treg

doi: 10.1007/s10565-025-10099-3

Figure Lengend Snippet: Macrophages stimulated by oxLDL affect the chemotaxis of Treg through CCL17 and CCL22 competition. Macrophages were stimulated with oxLDL, and Treg cells were co-cultured with macrophage supernatant. A Immunofluorescence detection of co-localization of CCL22 and macrophages (CD68) in plaque tissue of AS mice. B The content of CCL22 in the supernatant of macrophages was detected by ELISA. C Transwell detected the effect of macrophages on the chemotaxis of Treg. ( D - E ) Transwell detected the migration of Treg cells. * p < 0.05, ** p < 0.01, *** p < 0.001. N = 3

Article Snippet: Prior to the transwell migration assay, the induced Tregs were treated with recombinant mouse CCL17 (MCE, HY-P71891A) or recombinant mouse CCL22 (MCE, HY-P7248) or anti-CCL17 (R&D, Catalog #: MAB529) and anti-CCL22 antibodies (R&D, Catalog #: MAB529).

Techniques: Chemotaxis Assay, Cell Culture, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Migration

Journal: Nature Communications

Article Title: An elite allele TaDT1 - A hapI enhances drought tolerance via mediating autophagic pathways in wheat

doi: 10.1038/s41467-025-61943-3

Figure Lengend Snippet: a TaDT1-A displays transcriptional activation activity toward TaATG8 -promoter-LUC ( proATG8 -LUC) via the W-Box motif in wheat protoplasts ( n = 6 biological replicates). b Validation of TaDT1-A binding sites in the TaATG8 promoter under different treatment conditions in different plants via ChIP–qPCR analysis. The top diagram shows the fragments used for ChIP–qPCR ( n = 3 biological replicates). c EMSAs showing that TaDT1-A directly binds to the W-Box motif of the promoter of TaATG8 . The affinity-purified fusion protein GST-TaDT1-A was incubated with biotin-labeled probes. d Ten-day-old seedlings of the indicated genotypes were treated with or without 300 mM mannitol for 6 h and stained with MDC, and the middle zones of the leaves were observed via a confocal microscope (LSM880; Carl Zeiss, Heidenheim, Germany). Bar, 100 μm. The white triangle represents the autophagosome. e Representative transmission electron microscopy (TEM) images of autophagic structures in the mesophyll cells of transgenic plants subjected to different treatments. Red arrows indicate autophagic bodies. Bar, 5 μm. f Autophagosomes from ( d ) were quantified ( n = 3 biological replicates). g Drought stress tolerance of TaATG8 knockout transgenic, TaATG8 overexpression, D-ko12, D-ko12/A8-O2 and WT plants. Bar, 3 cm. h Ten-day-old seedlings of the indicated genotypes were treated with or without 300 mM mannitol for 6 h and stained with MDC, and the middle zones of the leaves were observed via a confocal microscope (LSM880; Carl Zeiss, Heidenheim, Germany). Bar, 100 μm. The white arrow represents the autophagosome. i Representative transmission electron microscopy (TEM) images of autophagic structures in the mesophyll cells of transgenic plants subjected to different treatments. Red arrows indicate autophagic bodies. Bars, 5 μm. Values are presented as means ± SD. For ( b ), *, **, and *** indicate significant differences at P < 0.05, P < 0.01, and P < 0.001 using the two-sided Student’s t -test. For ( a , f ), different letters were used to denote statistically significant differences, which were determined using two-way ANOVA with Tukey’s multiple comparisons test ( P < 0.05). Source data are provided as a file.

Article Snippet: Then the leaves of different genotypes in normal or drought conditions were excised and followed by incubation with 0.05 mM MDC (MedChemExpress, Monmouth Junction, NJ, USA) for 30 min in the dark.

Techniques: Activation Assay, Activity Assay, Biomarker Discovery, Binding Assay, ChIP-qPCR, Affinity Purification, Incubation, Labeling, Staining, Microscopy, Transmission Assay, Electron Microscopy, Transgenic Assay, Knock-Out, Over Expression