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Journal: ACS Omega
Article Title: Des-O-Methyllasiodiplodin Isolated from Xanthostemon chrysanthus Ameliorates Inflammation by Regulating Autophagy
doi: 10.1021/acsomega.5c12134
Figure Lengend Snippet: Regulatory effect of DML on autophagy in LPS-induced RAW264.7 cells. (A) Autophagosomes were determined by MDC staining under a confocal microscope. Scale bars, 25 μm. (B) The fluorescence intensity of autophagosomes by MDC staining per image in (A). The mRNA expression of LC3 B (C) and p62 (D). (E) LC3 I, LC3 II, and p62 protein levels. Internal reference was β-actin. (F) The statistical analysis of LC3 II/LC3 I and p62. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, and *** P < 0.001, compared with the control group. # p < 0.05 and ## p < 0.01, compared with the LPS group. § p < 0.05, §§§ p < 0.001, and §§§§ p < 0.0001, compared with the CQ group. @ p < 0.05 and @@@ p < 0.001, compared with the CQ + LPS group.
Article Snippet: Reactive Oxygen Species Assay Kit, DAF-FM DA probe, Autophagy Staining Assay Kit with
Techniques: Staining, Confocal, Microscopy, Fluorescence, Expressing, Analysis, Control