ipd3  (New England Biolabs)


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    Name:
    pMAL Protein Fusion and Purification System
    Description:
    pMAL Protein Fusion and Purification System
    Catalog Number:
    e8200s
    Price:
    677
    Category:
    E coli Protein Expression Kits
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    Structured Review

    New England Biolabs ipd3
    pMAL Protein Fusion and Purification System
    pMAL Protein Fusion and Purification System
    https://www.bioz.com/result/ipd3/product/New England Biolabs
    Average 90 stars, based on 16579 article reviews
    Price from $9.99 to $1999.99
    ipd3 - by Bioz Stars, 2020-07
    90/100 stars

    Images

    1) Product Images from "DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways"

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    Journal: Nature Communications

    doi: 10.1038/ncomms12433

    MtDELLAs can link IPD3 and NSP2. ( a , b ) The dmi3-1 , ipd3-2 , nsp1-2 and nsp2-1 mutants show defect in NIN and ERN1 expression. The RNA was extracted from 6 individual plants of wild type or mutants. Error bars represent standard error ( n =3, where n denotes the number of technical repetition). The asterisk indicates a significant decrease relative to the control with Student's t -test (* P
    Figure Legend Snippet: MtDELLAs can link IPD3 and NSP2. ( a , b ) The dmi3-1 , ipd3-2 , nsp1-2 and nsp2-1 mutants show defect in NIN and ERN1 expression. The RNA was extracted from 6 individual plants of wild type or mutants. Error bars represent standard error ( n =3, where n denotes the number of technical repetition). The asterisk indicates a significant decrease relative to the control with Student's t -test (* P

    Techniques Used: Expressing

    MtDELLAs promote root nodule symbiosis. ( a ) Whole-root GUS activity of p DELLA1:GUS , p DELLA2:GUS , p DELLA3:GUS , p NSP2:GUS , p IPD3:GUS are shown. Scale bars correspond to 2 mm. The black bars indicate that the region is selected for the transversal section in b . ( b ) Thin transversal sections (70 μm) of p DELLA1:GUS , p DELLA2:GUS , p DELLA3:GUS , p NSP2:GUS , p IPD3:GUS roots are shown. Scale bars correspond to 100 μm. ( c ) Numbers of nodules on MtDELLAs RNAi plant roots 4 weeks post inoculation with S. meliloti . n ⩾8, where n denotes the number of plants. ( d ) qRT-PCR analysis of MtDELLAs transcript levels. The expression levels of MtDELLAs were detected in wild-type (R108) and della1, della2 and della3 mutants. Expression levels were normalized against the reference gene Elongation factor 1-alpha ( EF1-α ). The RNA was extracted from six individual plants of R108 or mutants. ( e , f ) Scores of nodule number in della2 / della3 double mutants ( e ) and della1 / della2 / della3 triple mutants ( f ). ( g ) Quantification of infection threads in della double and triple mutants. The infection thread (IT) was visualized by staining of S. meliloti expressing the LacZ reporter and was counted at 7 d.p.i. n ⩾13, where n denotes the number of plants. Relative ITs density indicates the number of infection threads per root length per plant, and they were normalized against the wild-type (R108). ( h ) Nodule development is impaired in della1 / della2 / della3 triple mutant. There is no nodule formed in 12 plants out of 21 della1-1/della2/della3-1 triple mutants. Occasionally pink nodules were formed in della triple mutants. Scale bars correspond to 500 μm. ( i ) Nodule sections of the pink nodule and the white nodule in the della triple mutant, nodules were stained with toluidine blue. Scale bars correspond to 200 μm. This is a representative experiment repeated three times in c and d , and twice in e , f and g . Error bars represent s.d. The asterisk indicates a significant decrease relative to wild type or vector control with Student's t -test (* P
    Figure Legend Snippet: MtDELLAs promote root nodule symbiosis. ( a ) Whole-root GUS activity of p DELLA1:GUS , p DELLA2:GUS , p DELLA3:GUS , p NSP2:GUS , p IPD3:GUS are shown. Scale bars correspond to 2 mm. The black bars indicate that the region is selected for the transversal section in b . ( b ) Thin transversal sections (70 μm) of p DELLA1:GUS , p DELLA2:GUS , p DELLA3:GUS , p NSP2:GUS , p IPD3:GUS roots are shown. Scale bars correspond to 100 μm. ( c ) Numbers of nodules on MtDELLAs RNAi plant roots 4 weeks post inoculation with S. meliloti . n ⩾8, where n denotes the number of plants. ( d ) qRT-PCR analysis of MtDELLAs transcript levels. The expression levels of MtDELLAs were detected in wild-type (R108) and della1, della2 and della3 mutants. Expression levels were normalized against the reference gene Elongation factor 1-alpha ( EF1-α ). The RNA was extracted from six individual plants of R108 or mutants. ( e , f ) Scores of nodule number in della2 / della3 double mutants ( e ) and della1 / della2 / della3 triple mutants ( f ). ( g ) Quantification of infection threads in della double and triple mutants. The infection thread (IT) was visualized by staining of S. meliloti expressing the LacZ reporter and was counted at 7 d.p.i. n ⩾13, where n denotes the number of plants. Relative ITs density indicates the number of infection threads per root length per plant, and they were normalized against the wild-type (R108). ( h ) Nodule development is impaired in della1 / della2 / della3 triple mutant. There is no nodule formed in 12 plants out of 21 della1-1/della2/della3-1 triple mutants. Occasionally pink nodules were formed in della triple mutants. Scale bars correspond to 500 μm. ( i ) Nodule sections of the pink nodule and the white nodule in the della triple mutant, nodules were stained with toluidine blue. Scale bars correspond to 200 μm. This is a representative experiment repeated three times in c and d , and twice in e , f and g . Error bars represent s.d. The asterisk indicates a significant decrease relative to wild type or vector control with Student's t -test (* P

    Techniques Used: Activity Assay, Quantitative RT-PCR, Expressing, Infection, Staining, Mutagenesis, Plasmid Preparation

    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    CCaMK–IPD3–DELLA protein complex formation. ( a ) MtDELLAs increased the interaction between CCaMK and IPD3 in yeast three-hybrid assays. Data are presented as β-galactosidase activity. ( b ) CCaMK co-immunoprecipitates with IPD3 and DELLA2 in E. coli . CCaMK, IPD3 and MtDELLA2 are co-expressed in E. coli . ( c ) The effect of MtDELLAs on phosphorylation of IPD3 by CCaMK in vitro phosphorylation assays. The left histogram shows quantitative intensities of IPD3 phosphorylation calculated by ImageJ. The relative intensities were normalized against the control (bar1). ( d ) MtDELLA2 increased the phosphorylation of IPD3 by CCaMK. An increasing amount of MtDELLA2 was added to the reaction mix. The left histogram shows quantitative intensities of IPD3 phosphorylation calculated by ImageJ. The relative intensities were normalized against the bar1. Autoradiographs show the corresponding phosphorylated IPD3. Autoradiographs of kinase assays ( 32 P) (upper images) and Coomassie staining of the gels (lower image) in c and d . This is a representative experiment that was repeated twice in c and d . ( e ) CCaMK increased interaction of MtDELLA and IPD3 in yeast three-hybrid assay. Data are presented as β-galactosidase activity. ( f ) M. truncatula ipd3-2 roots were transformed with IPD3-S50A-S155A , IPD3-S50D-S155D and IPD3 . The pink nodules were formed on ipd3-2 mutants contained IPD3 and IPD3-S50D-S155D upon 35 day post inoculation with Sm1021 . Scale bars correspond to 500 μm. ( g ) Yeast three-hybrid assay showed sites S50 and S155 of IPD3 are critical for interaction of IPD3 and MtDELLA. Alanine replacement of S50 and S155 of IPD3 abolished the interaction with MtDELLA. Data are presented as β-galactosidase activity. Results represent the means of three experiments in a , e and g . Error bars represent standard error. The asterisk indicates a significant increase relative to the control with Student's t -test in a , e and g (* P
    Figure Legend Snippet: CCaMK–IPD3–DELLA protein complex formation. ( a ) MtDELLAs increased the interaction between CCaMK and IPD3 in yeast three-hybrid assays. Data are presented as β-galactosidase activity. ( b ) CCaMK co-immunoprecipitates with IPD3 and DELLA2 in E. coli . CCaMK, IPD3 and MtDELLA2 are co-expressed in E. coli . ( c ) The effect of MtDELLAs on phosphorylation of IPD3 by CCaMK in vitro phosphorylation assays. The left histogram shows quantitative intensities of IPD3 phosphorylation calculated by ImageJ. The relative intensities were normalized against the control (bar1). ( d ) MtDELLA2 increased the phosphorylation of IPD3 by CCaMK. An increasing amount of MtDELLA2 was added to the reaction mix. The left histogram shows quantitative intensities of IPD3 phosphorylation calculated by ImageJ. The relative intensities were normalized against the bar1. Autoradiographs show the corresponding phosphorylated IPD3. Autoradiographs of kinase assays ( 32 P) (upper images) and Coomassie staining of the gels (lower image) in c and d . This is a representative experiment that was repeated twice in c and d . ( e ) CCaMK increased interaction of MtDELLA and IPD3 in yeast three-hybrid assay. Data are presented as β-galactosidase activity. ( f ) M. truncatula ipd3-2 roots were transformed with IPD3-S50A-S155A , IPD3-S50D-S155D and IPD3 . The pink nodules were formed on ipd3-2 mutants contained IPD3 and IPD3-S50D-S155D upon 35 day post inoculation with Sm1021 . Scale bars correspond to 500 μm. ( g ) Yeast three-hybrid assay showed sites S50 and S155 of IPD3 are critical for interaction of IPD3 and MtDELLA. Alanine replacement of S50 and S155 of IPD3 abolished the interaction with MtDELLA. Data are presented as β-galactosidase activity. Results represent the means of three experiments in a , e and g . Error bars represent standard error. The asterisk indicates a significant increase relative to the control with Student's t -test in a , e and g (* P

    Techniques Used: Activity Assay, In Vitro, Staining, Hybrid Assay, Transformation Assay

    Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    2) Product Images from "DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways"

    Article Title: DELLA proteins are common components of symbiotic rhizobial and mycorrhizal signalling pathways

    Journal: Nature Communications

    doi: 10.1038/ncomms12433

    Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and MtCYCLOPS/IPD3. ( a ) Yeast two-hybrid assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pGADT7 (AD) and pGBKT7 (BD) vectors were plated onto SD/-Leu-Trp (-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound with HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP (N-terminal half of YFP) and IPD3-cYFP (C-terminal half of YFP). No protein interactions were detected in the other three combinations: MtDELLAs-nYFP--cYFP, IPD3-cYFP--nYFP and cYFP--nYFP. Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.
    Figure Legend Snippet: Interactions between MtDELLAs and NSP2. ( a ) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). ( b ) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. ( c ) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP ( Fig. 5c ). Scale bars, 20 μm.

    Techniques Used: Construct, Expressing, Bimolecular Fluorescence Complementation Assay, Fluorescence, Transformation Assay

    Related Articles

    Affinity Chromatography:

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    Purification:

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    Article Title: The Integrative Conjugative Element (ICE) of Mycoplasma agalactiae: Key Elements Involved in Horizontal Dissemination and Influence of Coresident ICEs
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    Article Title: Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase
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    Article Title: Structural and functional evaluation of de novo-designed, two-component nanoparticle carriers for HIV Env trimer immunogens
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    Produced:

    Article Title: The Integrative Conjugative Element (ICE) of Mycoplasma agalactiae: Key Elements Involved in Horizontal Dissemination and Influence of Coresident ICEs
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    Expressing:

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    Article Title: Characterization of Mannitol-2-Dehydrogenase in Saccharina japonica: Evidence for a New Polyol-Specific Long-Chain Dehydrogenases/Reductase
    Article Snippet: .. Recombinant Expression and Purification of SjM2DH pMAL Protein Fusion & Purification System (NEB #E8200S) was applied to perform the prokaryotic expression of SjM2DH in E. coli . .. The restriction sites of SjM2DH sequence were analyzed with the on-line tool WatCut ( http://watcut.uwaterloo.ca/watcut/watcut/template.php ).

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    Recombinant:

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    Over Expression:

    Article Title: Molecular and functional insights into a novel teleost malectin from big-belly seahorse Hippocampus abdominalis
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    New England Biolabs mbp dmi3
    Mbp Dmi3, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbp dmi3/product/New England Biolabs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mbp dmi3 - by Bioz Stars, 2020-07
    92/100 stars
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