maxima h minus m mulv reverse transcriptase  (Thermo Fisher)


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    Name:
    Maxima H Minus Reverse Transcriptase 200 U µL
    Description:
    Thermo Scientific Maxima H Minus Reverse Transcriptase RT was developed through in vitro evolution of M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M MuLV RT The engineered enzyme features dramatically improved thermostability 50X higher processivity robustness and increased synthesis rate compared to wild type M MuLV RT The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb Due to its high thermostability the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors such as guanidine formamide and ethanol Highlights• Thermostable 90 active after incubation at 50°C for 60 minutes in a reaction mixture • Active up to 65°C• High yields of full length cDNA up to 20 kb• High sensitivity reproducible cDNA synthesis from a wide range of starting total RNA amounts 1 pg to 5 µg • Efficient complete cDNA synthesis in 15 to 30 minutes• Increased resistance to common reaction inhibitors• Incorporates modified nucleotidesApplications• First strand cDNA synthesis for RT PCR and real time RT PCR • Reverse transcription at elevated temperatures to reduce effects of secondary structure • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • Analysis of RNA by primer extension Note• Also available Maxima H Minus First Strand cDNA Synthesis Kit
    Catalog Number:
    ep0751
    Price:
    None
    Applications:
    Cloning|PCR & Real-Time PCR|RT-PCR|Reverse Transcription|Two-Step RT-PCR|cDNA Libraries & Library Construction
    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher maxima h minus m mulv reverse transcriptase
    Thermo Scientific Maxima H Minus Reverse Transcriptase RT was developed through in vitro evolution of M MuLV RT The enzyme possesses an RNA dependent and DNA dependent polymerase activity but lacks RNase H activity due to mutation in RNase H domain of M MuLV RT The engineered enzyme features dramatically improved thermostability 50X higher processivity robustness and increased synthesis rate compared to wild type M MuLV RT The eliminated RNase H activity enables the enzyme to produce very long RNA transcripts up to 20 kb Due to its high thermostability the enzyme maintains full activity during the entire reverse transcription reaction and generates high yields of cDNA The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers The extremely high processivity of Maxima H Minus enzyme results in increased resistance to common reaction inhibitors such as guanidine formamide and ethanol Highlights• Thermostable 90 active after incubation at 50°C for 60 minutes in a reaction mixture • Active up to 65°C• High yields of full length cDNA up to 20 kb• High sensitivity reproducible cDNA synthesis from a wide range of starting total RNA amounts 1 pg to 5 µg • Efficient complete cDNA synthesis in 15 to 30 minutes• Increased resistance to common reaction inhibitors• Incorporates modified nucleotidesApplications• First strand cDNA synthesis for RT PCR and real time RT PCR • Reverse transcription at elevated temperatures to reduce effects of secondary structure • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • Analysis of RNA by primer extension Note• Also available Maxima H Minus First Strand cDNA Synthesis Kit
    https://www.bioz.com/result/maxima h minus m mulv reverse transcriptase/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    maxima h minus m mulv reverse transcriptase - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons
    Article Snippet: .. qRT-PCR was performed using KAPA SYBR FAST ABI Prism qPCR master mix (Kapa Biosystems) on cDNA synthesized using oligo (dT)18 primer and Maxima H-Minus reverse transcriptase (Thermo Scientific). cDNAs were generated from 1 μg of Trizol-isolated RNA from 10-20 pairs of dissected pupal retinas per biological replicate. .. Retinas were dissected on ice and tissue flash-frozen in Trizol.

    Generated:

    Article Title: Viral Polymerase-Helicase Complexes Regulate Replication Fidelity To Overcome Intracellular Nucleotide Depletion
    Article Snippet: .. To estimate the population diversity of variants by deep sequencing, cDNA libraries were prepared using Maxima H Minus reverse transcriptase (Invitrogen) from RNA extracted from virus generated and passaged three times in BHK-21 cells. .. The viral genome was PCR amplified by using a high-fidelity polymerase (Phusion) to generate one amplicon spanning the majority of the CHIKV genome.

    Article Title: Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons
    Article Snippet: .. qRT-PCR was performed using KAPA SYBR FAST ABI Prism qPCR master mix (Kapa Biosystems) on cDNA synthesized using oligo (dT)18 primer and Maxima H-Minus reverse transcriptase (Thermo Scientific). cDNAs were generated from 1 μg of Trizol-isolated RNA from 10-20 pairs of dissected pupal retinas per biological replicate. .. Retinas were dissected on ice and tissue flash-frozen in Trizol.

    Quantitative RT-PCR:

    Article Title: Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons
    Article Snippet: .. qRT-PCR was performed using KAPA SYBR FAST ABI Prism qPCR master mix (Kapa Biosystems) on cDNA synthesized using oligo (dT)18 primer and Maxima H-Minus reverse transcriptase (Thermo Scientific). cDNAs were generated from 1 μg of Trizol-isolated RNA from 10-20 pairs of dissected pupal retinas per biological replicate. .. Retinas were dissected on ice and tissue flash-frozen in Trizol.

    Real-time Polymerase Chain Reaction:

    Article Title: Wallenda/DLK protein levels are temporally downregulated by Tramtrack69 to allow R7 growth cones to become stationary boutons
    Article Snippet: .. qRT-PCR was performed using KAPA SYBR FAST ABI Prism qPCR master mix (Kapa Biosystems) on cDNA synthesized using oligo (dT)18 primer and Maxima H-Minus reverse transcriptase (Thermo Scientific). cDNAs were generated from 1 μg of Trizol-isolated RNA from 10-20 pairs of dissected pupal retinas per biological replicate. .. Retinas were dissected on ice and tissue flash-frozen in Trizol.

    Sequencing:

    Article Title: Viral Polymerase-Helicase Complexes Regulate Replication Fidelity To Overcome Intracellular Nucleotide Depletion
    Article Snippet: .. To estimate the population diversity of variants by deep sequencing, cDNA libraries were prepared using Maxima H Minus reverse transcriptase (Invitrogen) from RNA extracted from virus generated and passaged three times in BHK-21 cells. .. The viral genome was PCR amplified by using a high-fidelity polymerase (Phusion) to generate one amplicon spanning the majority of the CHIKV genome.

    Incubation:

    Article Title: Compartmentalized gut lymph node drainage dictates adaptive immune responses
    Article Snippet: .. The eluted sample was then incubated for 3 min at 72°C, followed by 1 min on ice, then 7.5 μl of mix 2 was added (3 μl 5X FS Buffer SS, 0.375 μl 100 mM DTT, 0.375 μl RNasin plus, 0.5 μl P1-RNA-TSO (40uM), 0.75 μl Maxima RT Minus H (Thermo Scientific, EP0751), 1.8 μl 5M Betaine (Sigma, B0300), 0.9 μl 50 mM MgCl2 and 0.175 μl RNase free ddH2 O. ..

    Polymerase Chain Reaction:

    Article Title: Molecular investigation of a full-length genome of a Q1-like IBV strain isolated in Italy in 2013.
    Article Snippet: .. Reverse transcription was performed with the commercial kit Maxima H Minus Reverse Transcriptase (ThermoFisher Scientific, Carlsbad, CA) while PCR was performed using the Phusion Hot Start II High-Fidelity DNA Polymerase kit (ThermoFisher Scientific, Carlsbad, CA). .. Both RT and PCR phases were thoroughly optimized with respect to primers (available on request), thermal profiles and reagents, so as to achieve an acceptable final yield in absence of non-specific products.

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  • 88
    Thermo Fisher maxima h minus reverse transcriptase 200 u µl
    Maxima H Minus Reverse Transcriptase 200 U µl, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/maxima h minus reverse transcriptase 200 u µl/product/Thermo Fisher
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    maxima h minus reverse transcriptase 200 u µl - by Bioz Stars, 2020-07
    88/100 stars
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