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    Name:
    Vascular Endothelial Growth Factor from mouse
    Description:
    Vascular endothelial growth factor is also known as VPF VEGF and MVCD1 It is secreted from endothelial cells and pericytes in response to hypoxia It helps in inducing angiogenesis and microvascular hyperpermeability VEGF levels are associated with vasculitic neuropathy and may be used to predict this disease It may also act as a marker in the development and progression of early precancerous lesions of oesophagus
    Catalog Number:
    v4512
    Price:
    None
    Applications:
    Vascular endothelial growth factor (VEGF) supports development of new blood vessels during embryonic development and after vascular injury.Vascular endothelial growth factor from mouse was used for ELISA and in matrigel plugs (matrigel was supplemented with VEGF).
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    Structured Review

    Millipore materials vegf a
    Influence of vascular endothelial growth factor <t>(VEGF),</t> axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p
    Vascular endothelial growth factor is also known as VPF VEGF and MVCD1 It is secreted from endothelial cells and pericytes in response to hypoxia It helps in inducing angiogenesis and microvascular hyperpermeability VEGF levels are associated with vasculitic neuropathy and may be used to predict this disease It may also act as a marker in the development and progression of early precancerous lesions of oesophagus
    https://www.bioz.com/result/materials vegf a/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    materials vegf a - by Bioz Stars, 2020-09
    99/100 stars

    Related Products / Commonly Used Together

    axitinib

    Images

    1) Product Images from "Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells"

    Article Title: Vascular Endothelial Growth Factor, Irradiation, and Axitinib Have Diverse Effects on Motility and Proliferation of Glioblastoma Multiforme Cells

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2017.00182

    Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p
    Figure Legend Snippet: Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the motility of glioblastoma multiforme cell lines U-251 and U-373. The motility of the cells was analyzed by time-laps videography. Cells were tracked and analyzed with the ibidi chemotaxis and migration tool. (A) Examples from an image stack of Videography. Some cells migrate fast (2), some slowly (3), some even do not migrate (1). Dividing cells (4) often keep contact over longer periods of time (4a, 4b). Scale bars: 50 µm. (B) Migration of U-373 glioblastoma cells under varied conditions. Depicted are the tracked cells in representative fields of view. The software merges all starting points in the origin to get an explicit view of the paths migrated by the cells. It is clearly visible that the migration is undirected (an advantage of videography over other methods to analyze migration). It is notable that some irradiated cells are able to escape the inhibition by axitinib. (C,D) The motility of U-251 and U-373 cells is increased by VEGF as well as by irradiation. In U-373, a combination of both leads to a significant increase in velocity compared to VEGF alone (D) , in U-251 no additive effects could be observed. In contrast, axitinib diminishes the motility of untreated cells and the elevated motility after irradiation as well. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. (E,F) 24 h after 2 Gy irradiation the amount of VEGF in the supernatant of U-251 and U-373 was analyzed. In U-251 there were no significant changes detectable, whereas in U-373 cells VEGF was significantly increased (F) . Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by * p

    Techniques Used: Irradiation, Chemotaxis Assay, Migration, Software, Inhibition

    Expression of vascular endothelial growth factor (VEGF)-R2 ( KDR ) in U-251 and U-373 glioblastoma cell lines. (A,B) VEGF-R1 ( FLT 1) as well as VEGF-R2 ( KDR) are expressed in both cell lines U-251 (A) and U-373 (B) . β -Actin was used as housekeeping gene. PCR products were isolated and confirmed by DNA sequencing. (C,D) Sequence of amplified PCR product for FLT1 (C) and KDR (D) matches their specific database entries ( FLT1 – NM_002019.4; KDR – NM_002253.2) and were proven to be unique in human glioblastoma cell lines U-251 as well as in U-373 by comparison with a database (Blast 2.2, U.S. National Centre for Biotechnology Information, Bethesda, MD, USA) (E) . Semi-quantitative analysis of gene expression normalized to β -Actin and compared to the expression of U-251-FLT (100%). Data are shown as mean ± SEM. n = 3. (F) Both glioblastoma multiforme cell lines express VEGF-R2 (green dots, arrows) in the cytoplasm as well as along the cell membrane. Counterstaining of the actin cytoskeleton is given in red as well as cell nuclei staining with DAPI in blue. Scale bars: 10 µm.
    Figure Legend Snippet: Expression of vascular endothelial growth factor (VEGF)-R2 ( KDR ) in U-251 and U-373 glioblastoma cell lines. (A,B) VEGF-R1 ( FLT 1) as well as VEGF-R2 ( KDR) are expressed in both cell lines U-251 (A) and U-373 (B) . β -Actin was used as housekeeping gene. PCR products were isolated and confirmed by DNA sequencing. (C,D) Sequence of amplified PCR product for FLT1 (C) and KDR (D) matches their specific database entries ( FLT1 – NM_002019.4; KDR – NM_002253.2) and were proven to be unique in human glioblastoma cell lines U-251 as well as in U-373 by comparison with a database (Blast 2.2, U.S. National Centre for Biotechnology Information, Bethesda, MD, USA) (E) . Semi-quantitative analysis of gene expression normalized to β -Actin and compared to the expression of U-251-FLT (100%). Data are shown as mean ± SEM. n = 3. (F) Both glioblastoma multiforme cell lines express VEGF-R2 (green dots, arrows) in the cytoplasm as well as along the cell membrane. Counterstaining of the actin cytoskeleton is given in red as well as cell nuclei staining with DAPI in blue. Scale bars: 10 µm.

    Techniques Used: Expressing, Polymerase Chain Reaction, Isolation, DNA Sequencing, Sequencing, Amplification, Staining

    Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the proliferation of glioblastoma multiforme cell lines U-251 and U-373. (A,B) Axitinib impairs the proliferation of irradiated and non-irradiated U-251 and U-373 cells. VEGF, irradiation, or the combination of both has no significant effect on cell proliferation. The proliferation of the cells was analyzed by a modified MTS-test. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by *** p
    Figure Legend Snippet: Influence of vascular endothelial growth factor (VEGF), axitinib, or irradiation with 2 Gy photons on the proliferation of glioblastoma multiforme cell lines U-251 and U-373. (A,B) Axitinib impairs the proliferation of irradiated and non-irradiated U-251 and U-373 cells. VEGF, irradiation, or the combination of both has no significant effect on cell proliferation. The proliferation of the cells was analyzed by a modified MTS-test. VEGF and axitinib were added in concentrations of 0.1 and 10 µg/ml, respectively. Data are shown as mean ± SEM. Data were tested for significance using one-way ANOVA with Bonferroni multiple comparison post-test. Significant differences are indicated by *** p

    Techniques Used: Irradiation, Modification

    Potential signaling in human glioblastoma cell lines after treatment with vascular endothelial growth factor (VEGF), irradiation and axitinib. VEGF activates multiple pathways including the Cdc42 and PLC pathways concerning cell migration and proliferation. It is supposed that stimulating effects of irradiation are mediated via enhanced synthesis of VEGF. Irradiation elevates VEGF biosynthesis of glioblastoma multiforme cells via MAPK activation. (A) Activation of the Cdc42 pathway by VEGF leads to an increased activation of Arp 2/3, HSP27, and ADF/cofilin resulting in an enhanced motility. Blockade of the VEGF-R2 by axitinib might decrease the activation of the Cdc42 pathway due to theoretical consideration resulting in a crucial decreased cell motility. (B) VEGF activates the PLC pathway which is involved in cell proliferation while axitinib might deactivate this pathway with impairment of cell proliferation. While motility is increased by VEGF, no positive effect on proliferation could be observed. This might be due to fully activated VEGF-pathways even under control conditions. Continuous arrows: evidence of the effect, dotted arrows: assumption of the effect.
    Figure Legend Snippet: Potential signaling in human glioblastoma cell lines after treatment with vascular endothelial growth factor (VEGF), irradiation and axitinib. VEGF activates multiple pathways including the Cdc42 and PLC pathways concerning cell migration and proliferation. It is supposed that stimulating effects of irradiation are mediated via enhanced synthesis of VEGF. Irradiation elevates VEGF biosynthesis of glioblastoma multiforme cells via MAPK activation. (A) Activation of the Cdc42 pathway by VEGF leads to an increased activation of Arp 2/3, HSP27, and ADF/cofilin resulting in an enhanced motility. Blockade of the VEGF-R2 by axitinib might decrease the activation of the Cdc42 pathway due to theoretical consideration resulting in a crucial decreased cell motility. (B) VEGF activates the PLC pathway which is involved in cell proliferation while axitinib might deactivate this pathway with impairment of cell proliferation. While motility is increased by VEGF, no positive effect on proliferation could be observed. This might be due to fully activated VEGF-pathways even under control conditions. Continuous arrows: evidence of the effect, dotted arrows: assumption of the effect.

    Techniques Used: Irradiation, Planar Chromatography, Migration, Activation Assay

    2) Product Images from "Influence of vascular endothelial growth factor and radiation on gap junctional intercellular communication in glioblastoma multiforme cell lines"

    Article Title: Influence of vascular endothelial growth factor and radiation on gap junctional intercellular communication in glioblastoma multiforme cell lines

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.219030

    Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on GJIC of U-87 cell lines. (A–F) VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Scale bar: 50 μm. (G) Quantitative results. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. *** P
    Figure Legend Snippet: Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on GJIC of U-87 cell lines. (A–F) VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Scale bar: 50 μm. (G) Quantitative results. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. *** P

    Techniques Used: Irradiation

    Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on cell proliferation of U-87 cells (A) and influence of VEGF combined with axitinib on U-251 cell proliferation (B). VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test.*** P
    Figure Legend Snippet: Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on cell proliferation of U-87 cells (A) and influence of VEGF combined with axitinib on U-251 cell proliferation (B). VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test.*** P

    Techniques Used: Irradiation

    Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on GJIC of U-251 cell lines. (A–F) VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Scale bar: 50 μm. (G) Quantitative results. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. * P
    Figure Legend Snippet: Influence of VEGF, the VEGF-R2 inhibitor axitinib, or irradiation with 2 Gy photons on GJIC of U-251 cell lines. (A–F) VEGF was added at 0.1 μg/mL, axitinib at 100 μg/mL. Scale bar: 50 μm. (G) Quantitative results. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. * P

    Techniques Used: Irradiation

    Influence of VEGF and VEGF combined with the VEGF-R2 inhibitor axitinib on GJIC of U-251 cell lines. VEGF was added in a concentration of 0.1 μg/mL, axitinib of 100 μg/mL. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. *** P
    Figure Legend Snippet: Influence of VEGF and VEGF combined with the VEGF-R2 inhibitor axitinib on GJIC of U-251 cell lines. VEGF was added in a concentration of 0.1 μg/mL, axitinib of 100 μg/mL. Data were expressed as the mean ± standard error, and analyzed by unpaired t -test. *** P

    Techniques Used: Concentration Assay

    Related Articles

    Immunohistochemistry:

    Article Title: Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases
    Article Snippet: .. Immunohistochemistry and immunocytochemistry Proliferation (PCNA; rabbit polyclonal, Santa Cruz sc-7907 ), apoptosis (active caspase 3; rabbit polyclonal, R & D Systems AF835 ), angiogenesis (CD34 neovascularisation marker; rat anti-mouse, Abd Serotec MCA18256), and VEGF (CalBiochem, PC315) were assessed in PFA-fixed paraffin embedded tissues. .. PCNA was used at the concentration of 0.143 μg/ml, active caspase-3 at 1.0 μg/ml, CD34 at 0.1 μg/ml, and VEGF at 1.5 μg/ml.

    Immunocytochemistry:

    Article Title: Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases
    Article Snippet: .. Immunohistochemistry and immunocytochemistry Proliferation (PCNA; rabbit polyclonal, Santa Cruz sc-7907 ), apoptosis (active caspase 3; rabbit polyclonal, R & D Systems AF835 ), angiogenesis (CD34 neovascularisation marker; rat anti-mouse, Abd Serotec MCA18256), and VEGF (CalBiochem, PC315) were assessed in PFA-fixed paraffin embedded tissues. .. PCNA was used at the concentration of 0.143 μg/ml, active caspase-3 at 1.0 μg/ml, CD34 at 0.1 μg/ml, and VEGF at 1.5 μg/ml.

    In Vitro:

    Article Title: Morphological Plasticity of Emerging Purkinje Cells in Response to Exogenous VEGF
    Article Snippet: .. In Vitro Treatment of PCs The impact of VEGF (V4512, Sigma Aldrich) on the morphology of PC dendrites was investigated in cerebellar slice cultures of p1 rats. .. In comparison to controls, slice cultures were incubated with VEGF (c = 0.1 μg/ml) nutrient medium as described previously (Jin et al., ), with a mixture of VEGF (c = 0.1 μg/ml) and axitinb (c = 0.01 mg/ml), or solely with axitinib (c = 0.01 mg/ml) for 48 h. Afterwards they were fixed with 4% paraformaldehyde (PFA) for 1 h and washed with phosphate-buffered saline (PBS).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Obligate Roles for p16Ink4a and p19Arf-p53 in the Suppression of Murine Pancreatic Neoplasia
    Article Snippet: .. TGF-α and vascular endothelial growth factor (VEGF) levels were measured using enzyme-linked immunosorbent assay (ELISA) kits (Oncogene Research). ..

    Concentration Assay:

    Article Title: Triple-marker cardiac MRI detects sequential tissue changes of healing myocardium after a hydrogel-based therapy
    Article Snippet: .. After one hour of stirring, the liquefied UPy-hydrogel was mixed with mouse recombinant VEGF and IGF1 (V4512–5UG and I8779–50UG, Sigma-Aldrich, St Louis, MO, USA) to a concentration of 500 ng/ml of both GF. .. In addition, a second batch of the UPyGF -hydrogel was mixed with 13,6 μg/ml USPIOS (Sinerem, Guerbet, Villepoint, France) for in vivo tracking after delivery.

    Incubation:

    Article Title: THE NOVEL LUPUS ANTIGEN RELATED PROTEIN ACHERON ENHANCES THE DEVELOPMENT OF HUMAN BREAST CANCER
    Article Snippet: .. Proteins were transferred to a nylon membrane (Invitrogen, Carlsbad, CA) and incubated with one of several antisera: rabbit anti-Achn (1:100; Schwartz laboratory), rabbit anti-GFP (1:500) (Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-MMP-9 (1:500), (VWR, West Chester, PA), VEGF (1:200) (Sigma, St. Loius, MO), and actin (1:1000) (Sigma). .. HRP-labeled goat anti-rabbit or mouse antisera (1:10,000) and an ECL kit (VWR) were used for detection.

    Article Title: Rational design of temperature-sensitive blood-vessel-embolic nanogels for improving hypoxic tumor microenvironment after transcatheter arterial embolization
    Article Snippet: .. Endogenous peroxidase was blocked with methanol containing 3% hydrogen peroxide for 10 min. For various immunofluorescent analyses, the slices were incubated overnight at 4 o C with a 150-fold dilution of mouse anti-Ki67 antigen (Dako, Carpinteria, CA, USA), a 100-fold dilution of mouse anti-HIF-1α (Thermo, IL, USA), a 150-fold dilution of mouse anti-VEGF (Millipore, Billerica, MA, USA), and a 50-fold dilution of mouse anti-human CD31 (Dako, Copenhagen, Denmark) monoclonal antibodies. .. The slices were washed with PBS 3 times for 5 min each and incubated at 37 o C sequentially with a 150-fold dilution of goat anti-rabbit secondary antibody (Aspen, Wuhan, China) for 50 min and a 200-fold dilution of fluorescent secondary antibody fluorescein isothiocyanate (FITC) (1 : 200, Wuhan Servicebio Biological Technology Co., Ltd., Wuhan, China) or Cyanine 3 (Cy3) (1 : 50, Wuhan Servicebio Biological Technology Co., Ltd.) for 60 min. Lastly, the slices were stained with DAPI to label the nuclei of tumor cells.

    Marker:

    Article Title: Targeting the angiotensin II type 2 receptor (AT2R) in colorectal liver metastases
    Article Snippet: .. Immunohistochemistry and immunocytochemistry Proliferation (PCNA; rabbit polyclonal, Santa Cruz sc-7907 ), apoptosis (active caspase 3; rabbit polyclonal, R & D Systems AF835 ), angiogenesis (CD34 neovascularisation marker; rat anti-mouse, Abd Serotec MCA18256), and VEGF (CalBiochem, PC315) were assessed in PFA-fixed paraffin embedded tissues. .. PCNA was used at the concentration of 0.143 μg/ml, active caspase-3 at 1.0 μg/ml, CD34 at 0.1 μg/ml, and VEGF at 1.5 μg/ml.

    Injection:

    Article Title: A novel antiangiogenic peptide derived from hepatocyte growth factor inhibits neovascularization in vitro and in vivo
    Article Snippet: .. PBS and anti-mouse VEGF antibodies (VEGFab, a neutralizing antibody to VEGF that recognizes the mouse; Sigma-Aldrich) were injected as controls. .. PBS and peptide injections were performed twice on P12 and P14, and a VEGFab injection was given once on P12, according to Geisen et al. [ ].

    Recombinant:

    Article Title: Triple-marker cardiac MRI detects sequential tissue changes of healing myocardium after a hydrogel-based therapy
    Article Snippet: .. After one hour of stirring, the liquefied UPy-hydrogel was mixed with mouse recombinant VEGF and IGF1 (V4512–5UG and I8779–50UG, Sigma-Aldrich, St Louis, MO, USA) to a concentration of 500 ng/ml of both GF. .. In addition, a second batch of the UPyGF -hydrogel was mixed with 13,6 μg/ml USPIOS (Sinerem, Guerbet, Villepoint, France) for in vivo tracking after delivery.

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    Millipore materials recombinant human vascular endothelial growth factor 165
    Materials Recombinant Human Vascular Endothelial Growth Factor 165, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/materials recombinant human vascular endothelial growth factor 165/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    materials recombinant human vascular endothelial growth factor 165 - by Bioz Stars, 2020-09
    96/100 stars
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