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Eppendorf AG mastercycler epgradient thermalcyclers
Mastercycler Epgradient Thermalcyclers, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mastercycler epgradient thermalcyclers/product/Eppendorf AG
Average 78 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mastercycler epgradient thermalcyclers - by Bioz Stars, 2020-02
78/100 stars

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DNA Extraction:

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: Paragraph title: DNA extraction and genotyping ... The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany).

Amplification:

Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
Article Snippet: Paragraph title: PCR amplification and SNP discovery ... All PCRs and sequencing reactions were carried out in 96-well plates (MicroAmp® 96-well plates, Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany) and each reaction was performed in 10 μl with the following mixture composition: 20 ng of DNA, 1.5 mM MgCl2 , 0.4 μM of each primer and 0.25 U Taq HotStart DNA polymerase QIAGEN (Valencia, USA).

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: .. The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany). .. Each reaction was performed in 10 μl with the following mixture composition: 20 ng of DNA, 1.5 mM of MgCl2 , 0.4 μM of the first primer, 0.2 μM of the second primer with M13 complementary tail, 0.2 μM of M13 fluorescent labelled primer, and 0.25 U of Taq HotStart DNA polymerase QIAGEN (Valencia, USA).

Isolation:

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: DNA extraction and genotyping Leaves from 5 plants were pooled and genomic DNA was isolated from 6 mg of lyophilized material in 96-well microtube plates using Macherey Nagel® NucleoSpin Plant II kit and following manufacturer's instructions. .. The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany).

Sequencing:

Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
Article Snippet: .. All PCRs and sequencing reactions were carried out in 96-well plates (MicroAmp® 96-well plates, Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany) and each reaction was performed in 10 μl with the following mixture composition: 20 ng of DNA, 1.5 mM MgCl2 , 0.4 μM of each primer and 0.25 U Taq HotStart DNA polymerase QIAGEN (Valencia, USA). .. The reaction mixtures for Vrn-H1 and HvCbf6 amplifications were supplemented with 3 mM of MgCl2 and 1× Q Solution QIAGEN (Valencia, USA), respectively.

Concentration Assay:

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: The quality and the concentration of the genomic DNA were assessed using electrophoretic analysis and Picogreen® technology (Invitrogen, San Diego, CA), respectively. .. The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany).

Polymerase Chain Reaction:

Article Title: Genetic variants of HvCbf14 are statistically associated with frost tolerance in a European germplasm collection of Hordeum vulgare
Article Snippet: Paragraph title: PCR amplification and SNP discovery ... All PCRs and sequencing reactions were carried out in 96-well plates (MicroAmp® 96-well plates, Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany) and each reaction was performed in 10 μl with the following mixture composition: 20 ng of DNA, 1.5 mM MgCl2 , 0.4 μM of each primer and 0.25 U Taq HotStart DNA polymerase QIAGEN (Valencia, USA).

Article Title: Molecular diversity, population structure, and linkage disequilibrium in a worldwide collection of tobacco (Nicotiana tabacum L.) germplasm
Article Snippet: All SSR loci considered in this study were amplified using a three-primer system for indirect labelling PCR fragments [ ]. .. The amplification of SSR loci was carried out in 384-well plates (Applied Biosystems, Foster City, USA) in Eppendorf Mastercycler EPgradient thermalcyclers (Eppendorf, Hamburg, Germany).

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    Eppendorf AG mastercycler epgradient thermalcyclers
    Mastercycler Epgradient Thermalcyclers, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mastercycler epgradient thermalcyclers/product/Eppendorf AG
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mastercycler epgradient thermalcyclers - by Bioz Stars, 2020-02
    78/100 stars
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