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Journal: eLife
Article Title: Human receptive endometrial assembloid for deciphering the implantation window
doi: 10.7554/eLife.90729
Figure Lengend Snippet: ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).
Article Snippet: Antibody ,
Techniques: Construct, Cell Culture, Gene Expression, Transformation Assay, Biomarker Discovery, Immunofluorescence, In Vitro, Comparison
Journal: The FASEB Journal
Article Title: Methylglyoxal Affects Dopamine Homeostasis in SH‐SY5Y Cells Through the Modulation of miR‐190a and miR‐214
doi: 10.1096/fj.202501805R
Figure Lengend Snippet: MAO‐B, COMT and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
Article Snippet: The primary antibodies used were: anti‐Carboxymethyl Lysine antibody (Abcam, Trumpington, Cambridge, UK #27684), anti‐MGO (Abcam, Trumpington, Cambridge, UK #243074), DDC (DDC D6N8N, Cell Signaling, Leiden, Netherlands, #13561); TH (monoclonal anti‐Tyrosine hydroxylase clone TH‐2, Sigma Aldrich #T1299); COMT (COMT D4N6M, Cell Signaling #14368S); α‐Synuclein (α‐Synuclein Antibody, Cell Signaling #2642S);
Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control