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93
Santa Cruz Biotechnology mao a
( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), <t>n=4</t> <t>(MAO-A</t> and DPP4).
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Santa Cruz Biotechnology sc 271123 rrid ab 10609510
( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), <t>n=4</t> <t>(MAO-A</t> and DPP4).
Sc 271123 Rrid Ab 10609510, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology mao b
<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
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Proteintech rabbit anti mao b
<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
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<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
Anti Mao B, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology anti mao b antibody
<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
Anti Mao B Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology anti mao b
<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
Anti Mao B, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mao b/product/Santa Cruz Biotechnology
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Santa Cruz Biotechnology primary antibodies against maoa
<t>MAO‐B,</t> <t>COMT</t> and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.
Primary Antibodies Against Maoa, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).

Journal: eLife

Article Title: Human receptive endometrial assembloid for deciphering the implantation window

doi: 10.7554/eLife.90729

Figure Lengend Snippet: ( A ) Human endometrial assembloids constructed from adult stem cells were treated with expansion medium (ExM) (CTRL) or subjected to hormonal stimulation. Timeline of endometrial assembloid cultured by ExM (CTRL), ovarian steroid hormones simulating secretory phase (SEC), ovarian steroid hormones combining PRL and placental hormones to mimic the window of implantation (WOI). ( B ) Endometrial assembloids from the CTRL, SEC, and WOI groups, which were subjected to hormone treatment on Days 0, 2, and 8, exhibited comparable growth patterns throughout the culture period. Scale bar = 200 μm. ( C ) The dynamic changes of the counts of assembloids over time in each hormone regimen. ( D ) The dynamic changes of the area of assembloids over time in each hormone regimen. ( E ) Heatmap showing receptivity-related gene expression profile of assembloids in each hormone regimen. The color represents log-transformed fold change of gene expression. ( F ) Validation of receptivity markers (IGFBP1, MAOA, and DPP4) with immunofluorescence (IF) in the CTRL, SEC, and WOI endometrial assembloids in vitro. Nuclei were counterstained with DAPI. Scale bar = 30 μm. The bar chart displays the quantitative comparison of receptivity markers among three groups. * p ≤0.05, ** p ≤0.005, *** p ≤0.0005, **** p ≤0.0001.n=4 (CTRL) and 5 (SEC and WOI) (IGFBP1), n=4 (MAO-A and DPP4).

Article Snippet: Antibody , MAO-A (Mouse monoclonal) , Santa Cruz , Cat#: sc-271123 RRID: AB_10609510 , IF (1:50).

Techniques: Construct, Cell Culture, Gene Expression, Transformation Assay, Biomarker Discovery, Immunofluorescence, In Vitro, Comparison

MAO‐B, COMT and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.

Journal: The FASEB Journal

Article Title: Methylglyoxal Affects Dopamine Homeostasis in SH‐SY5Y Cells Through the Modulation of miR‐190a and miR‐214

doi: 10.1096/fj.202501805R

Figure Lengend Snippet: MAO‐B, COMT and α‐Syn expression in response to MGO. MAO‐B (a) ( n = 4), COMT (c) ( n = 4), and SNCA (e) ( n = 4) mRNA expression were measured by real‐time PCR in differentiated SH‐SY5Y cells treated or not with 400 μM MGO for 48 h. MAO‐B (b) ( n = 4), COMT (d) ( n = 4) and α‐Syn (f) ( n = 4) protein levels were measured by Western blot using Vinculin or β‐Actin as loading control. A representative blot and the densitometric analysis are shown.

Article Snippet: The primary antibodies used were: anti‐Carboxymethyl Lysine antibody (Abcam, Trumpington, Cambridge, UK #27684), anti‐MGO (Abcam, Trumpington, Cambridge, UK #243074), DDC (DDC D6N8N, Cell Signaling, Leiden, Netherlands, #13561); TH (monoclonal anti‐Tyrosine hydroxylase clone TH‐2, Sigma Aldrich #T1299); COMT (COMT D4N6M, Cell Signaling #14368S); α‐Synuclein (α‐Synuclein Antibody, Cell Signaling #2642S); MAO‐B (MAO‐B D‐6, Santa Cruz Biotechnology, Dallas, Texas, USA, #sc515354); β‐actin (Santa Cruz Biotechnology #sc47778); vinculin (7F9, Santa Cruz Biotechnology #sc73614); secondary antibodies were: anti‐mouse (Goat anti Mouse IgG (H/L): HRP, Bio‐Rad #STAR117P); anti‐rabbit (Goat anti Rabbit IgG (H/L):HRP, Bio‐Rad #STAR124P).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Control