Journal: AMB Express

Article Title: Anti-furfurative comparison of Kesh Kanti-Herbal Shampoos and synthetic shampoos against Malassezia furfur for dandruff management

doi: 10.1186/s13568-024-01818-w

Figure Lengend Snippet: Antimicrobial activity of shampoos against Malassezia furfur. Antifungal activity of various shampoos against M. furfur determined by disc diffusion assay. ( A ) Representative Dixon agar plates showing the growth of M. furfur and the resulting clear zones (highlighted with white circles) formed around the sterile discs loaded with shampoos diluted in sterile deionized water at a concentration (v/v) of 1%, 5%, 10%, 20% and 30%. Control and test shampoos: (a) Disc chart indicating shampoo concentration on each disc. C, is the control sterile disc loaded with sterile deionized water. Plates showing disc diffusion with indicated shampoo at the concentrations shown in the disc chart (b) KETO (2% Ketoconazole-containing shampoo, comparator) (c) HS_Adv (d) HS_M&P (e) HS_Aloe; (f) SYN_01 (g) SYN_02 (h) SYN_03. The zones of inhibition around each disc measured and represented ( B ) Graphical representation of diameter (millimeters, mm) of clear zone from by each shampoo ( n = 3). Error bars represent mean ± SD; the significance of data calculated with respect to zone of inhibition formed by respective shampoos at 1% dilution as, # p < 0.0001, *** p < 0.0005, ** p < 0.005, * p < 0.05 and ns, non-significant

Article Snippet: The Malassezia furfur was cultured on Dixon agar (HiMedia laboratories Pvt Ltd, Mumbai, India) containing Malt Extract 36.0 g/L, Peptone 36.0 g/L, Bile Desiccated 20.0 g/L, Tween 40 10.0 g/L and Glycerol mono-oleate 5.0 g/L.

Techniques: Activity Assay, Diffusion-based Assay, Sterility, Concentration Assay, Control, Inhibition

Journal: AMB Express

Article Title: Anti-furfurative comparison of Kesh Kanti-Herbal Shampoos and synthetic shampoos against Malassezia furfur for dandruff management

doi: 10.1186/s13568-024-01818-w

Figure Lengend Snippet: A brief 3-minute contact with shampoo is sufficient to curtail the viability of Malassezia furfur. ( A ) Experimental design to study the viability of M. furfur after a brief 3-minute incubation with 1% and 5% shampoo dilution. After incubation, the yeast cells were diluted and plated on Dixon agar plate for viability assessment ( B ) Representative images of M. furfur colonies on agar plates after treatment with various shampoos at 1% and 5% dilutions: Herbal shampoo group: HS_Adv, HS_M&P, HS_Aloe; synthetic shampoo group: SYN_01, SYN_02, SYN_03; and KETO (2% Ketoconazole containing shampoo, comparator), and UT (Untreated control). The reduction in the number of colonies indicates the efficacy of the shampoos in inhibiting fungal growth

Article Snippet: The Malassezia furfur was cultured on Dixon agar (HiMedia laboratories Pvt Ltd, Mumbai, India) containing Malt Extract 36.0 g/L, Peptone 36.0 g/L, Bile Desiccated 20.0 g/L, Tween 40 10.0 g/L and Glycerol mono-oleate 5.0 g/L.

Techniques: Incubation, Control

Journal: AMB Express

Article Title: Anti-furfurative comparison of Kesh Kanti-Herbal Shampoos and synthetic shampoos against Malassezia furfur for dandruff management

doi: 10.1186/s13568-024-01818-w

Figure Lengend Snippet: Malassezia furfur recovers from the 3-minute shampoo contact within 72 h ( A ) Experimental design to study the potential of M. furfur to recover from the brief 3-minute exposure with 1% and 5% shampoo dilutions. Immediately after shampoo exposure, M. furfur was incubated in growth media. Culture recovery was assessed by monitoring the absorbance at 600 nm followed by viability determination after 72 h by plating cultures on Dixon agar plates. ( B ) Optical density (absorbance) measurements at 600 nm indicate the growth of M. furfur after 24 h, 48 h, and 72 h of 1% shampoo contact. ( C ) Optical density (absorbance) measurements at 600 nm indicate the growth of M. furfur after 24 h, 48 h, and 72 h of 5% shampoo contact. ( D ) Representative images of M. furfur colonies on agar plates of culture recovered by 72 h of 3-minute contact with various shampoos: Herbal shampoo group: HS_Adv, HS_M&P, HS_Aloe; synthetic shampoo group: SYN_01, SYN_02, SYN_03; and KETO (2% Ketoconazole containing shampoo, comparator), and UT (Untreated). A decrease in colony number and optical density indicates effective inhibition of fungal regrowth. Error bars represent mean ± SD; the significance of data calculated with respect to untreated (UT) as, # p < 0.0001, *** p < 0.0005, ** p < 0.005, * p < 0.05 and ns, non-significant ( n = 3)

Article Snippet: The Malassezia furfur was cultured on Dixon agar (HiMedia laboratories Pvt Ltd, Mumbai, India) containing Malt Extract 36.0 g/L, Peptone 36.0 g/L, Bile Desiccated 20.0 g/L, Tween 40 10.0 g/L and Glycerol mono-oleate 5.0 g/L.

Techniques: Incubation, Inhibition

Journal: AMB Express

Article Title: Anti-furfurative comparison of Kesh Kanti-Herbal Shampoos and synthetic shampoos against Malassezia furfur for dandruff management

doi: 10.1186/s13568-024-01818-w

Figure Lengend Snippet: Recurrent exposure to both herbal and synthetic shampoos impacts the growth, recovery and viability of M. furfur. ( A ) Schematic illustrating the experimental design wherein M. furfur cultures were incubated for 3 min with 1% and 5% shampoo dilutions (1st exposure), followed by 24 h recovery in growth media. The next day, the recovered culture was subjected to absorbance-based growth assessment and viability testing by dropping the equal volume of cultures. After absorbance and patch plating, the leftover cultures were again incubated with 1% and 5% shampoo dilutions for 3 min (2nd exposure), followed by recovery as above. After 24 h, subsequently, after growth and viability evaluation, the yeast cells were again incubated with 1% and 5% shampoo dilutions for 3 min (3rd exposure), followed by recovery for another 24 h. Next day, absorbance-based growth assessment and viability testing by patch plating were done. ( B ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 1st shampoo exposure at 1% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). ( C ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 1st shampoo exposure at 5% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). ( D ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 2nd shampoo exposure at 1% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). ( E ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 2nd shampoo exposure at 5% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). ( F ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 3rd shampoo exposure at 1% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). ( G ) Graph representing absorbance (at 600 nm) of the culture after 24 h recovery following 3rd shampoo exposure at 5% dilution. Patch plating results of the cultures at 3 dilutions, 10 −2 (top lane), 10 −1 (middle lane) and no dilution (lower lane). The values in the graph represent Error bars represent mean ± SD; the significance of data calculated with respect to untreated (UT) as, # p < 0.0001 and ns, non-significant ( n = 3)

Article Snippet: The Malassezia furfur was cultured on Dixon agar (HiMedia laboratories Pvt Ltd, Mumbai, India) containing Malt Extract 36.0 g/L, Peptone 36.0 g/L, Bile Desiccated 20.0 g/L, Tween 40 10.0 g/L and Glycerol mono-oleate 5.0 g/L.

Techniques: Incubation

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Evaluation of Malassezia furfur pathogenicity in Caenorhabditis elegans . (A) Survival curves of N2 (wild-type) worms fed Escherichia coli OP50 (OP, n = 55) or M. furfur ( M. furfur , n = 55). (B) Survival curves of N2 worms fed live OP or live M. furfur , and heat-killed (HK)-OP or HK- M. furfur (OP, n = 78; HK-OP, n = 69; M. furfur , n = 58; HK- M. furfur , n = 43). For (A) and (B), young adult worms (3-d-old) represent day 0 of observation. Survival rates were calculated using the Kaplan–Meier method and asterisks indicate a significant difference compared to OP-fed worms (control) using the log-rank (Mantel-Cox) test. ***, P < 0.001. (C) Body size of worms fed OP, HK-OP, M. furfur , or HK- M. furfur from 4 to 6 d of age. Asterisks indicate significant differences compared to OP-fed worms (control). (D) The intestinal barrier function was assessed by examining the percentage of worms showing leakage of dye into the body-cavity (Smurf assay). Worms fed OP, HK-OP, M. furfur , or HK- M. furfur were examined from 4 to 6 d of age. For (C) and (D), results are shown as individual plots and means ± standard error of mean (SEM). Statistical analysis was performed with one-way ANOVA and Tukey’s multiple comparison tests. ***, P < 0.001; **, P < 0.01; *, P < 0.05. (E) Representative images of 6-d-old worms fed OP, HK-OP, M. furfur , or HK- M. furfur and stained with blue dye. Scale bar, 100 µm.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques: Control, Comparison, Staining

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Lifespan assay of Caenorhabditis elegans loss-of-function mutants fed Malassezia furfur . (A) Survival curves of N2 (wild-type) and nsy-1 loss-of-function mutant worms fed M. furfur (N2, n = 43; nsy-1 , n = 40). (B) Survival curves of N2 and p38 pathway loss-of-function mutants ( sek-1 and pmk-1 ) worms fed M. furfur (N2, n = 56; sek-1 , n = 53; pmk-1 , n = 60). (C) Survival curves of N2 and c-Jun N-Terminal Kinase (JNK) pathway loss-of-function mutants ( mkk-4 and jnk-1 ) worms fed M. furfur (N2, n = 38; mkk-4 , n = 28; jnk-1 , n = 61). For (A) through (C), young adult worms (3-d-old) represent day 0 of observation. Survival rates were calculated using the Kaplan–Meier method and asterisks indicate a significant difference compared to N2 worms (control) using the log-rank (Mantel-Cox) test. ***, P < 0.001; **, P < 0.01; *, P < 0.05; N.S., not significant.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques: Mutagenesis, Control

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Expression of genes related to top-ranked Gene Ontology terms that were enriched in the N2 (wild-type) Malassezia furfur -fed Caenorhabditis elegans group. Genes related to C-type lectin domain-containing protein (A), antimicrobial peptide (B), other functions in response to biotic stimulus excluding those for C-type lectin or antimicrobial peptide (C), and unknown detailed function (D) are shown. For (A) through (D), real-time PCR was used to determine mRNA expression levels of genes in N2 and nsy-1 loss-of-function mutants fed Escherichia coli OP50 (OP) or M. furfur relative to the control group (N2_OP). Data are presented as means ± standard error of mean (SEM) of three independent experiments, each normalized to three reference genes ( act-1 , cyc-1 , or tba-1 ). Asterisks indicate statistically significant differences between N2_OP and N2_ M. furfur groups. ***, P < 0.001; **, P < 0.01; *, P < 0.05. Daggers indicate statistically significant differences between N2_ M. furfur and nsy-1 _ M. furfur groups. †††, P < 0.001; ††, P < 0.01; †, P < 0.05.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Effects of lactic acid bacteria on the lifespan of Caenorhabditis elegans fed Malassezia furfur. (A) Survival curves of N2 (wild-type) worms fed with a 1:1 mixture of Escherichia coli OP50 and M. furfur (OP + M. furfur (1:1), n = 74), a 1:1 mixture of Lacticaseibacillus rhamnosus and M. furfur (LR + M. furfur (1:1), n = 59), or only M. furfur ( M. furfur , n = 39). (B) Survival curves of N2 worms fed a 1:1 mixture of OP and M. furfur (OP + M. furfur (1:1), n = 78), a 1:1 mixture of Lactobacillus helveticus and M. furfur (LH + M. furfur (1:1), n = 51), a 1:1 mixture of Lactiplantibacillus plantarum and M. furfur (LP + M. furfur (1:1), n = 63), or only M. furfur ( M. furfur , n = 43). For (A) and (B), young adult worms (3-d-old) represent day 0 of observation. Survival rates were calculated using the Kaplan–Meier method and asterisks indicate a significant difference compared to OP + M. furfur -fed worms, using the log-rank (Mantel–Cox) test. ***, P < 0.001; **, P < 0.01.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques: Bacteria

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Evaluation of lactic acid bacteria protection against Malassezia furfur virulence in Caenorhabditis elegans . (A) Survival curves of N2 (wild-type) worms fed only Escherichia coli OP50 (OP, n = 77), a 1:1 mixture of OP and M. furfur (OP + M. furfur (1:1), n = 79), a 1:1 mixture of Lacticaseibacillus rhamnosus and M. furfur (LR + M. furfur (1:1), n = 50), or only M. furfur ( M. furfur , n = 43). (B) Survival curves of N2 worms fed a mixture with a high proportion of M. furfur (OP, n = 79; OP + M. furfur (1:4), n = 79; LR + M. furfur (1:4), n = 55; M. furfur , n = 46). For (A) and (B), young adult worms (3-d-old) represent day 0 of observation. Survival rates were calculated using the Kaplan–Meier method and asterisks indicate a significant difference compared to OP + M. furfur -fed worms using the log-rank (Mantel–Cox) test. ***, P < 0.001; *, P < 0.05. (C) Body size of worms fed OP, OP + M. furfur (1:4), LR + M. furfur (1:4), or only M. furfur from 4 to 6 d of age. Asterisks indicate significant differences compared to OP + M. furfur (1:4)-fed worms. (D) The percentage of body-cavity leakage worms fed OP, OP + M. furfur (1:4), or LR + M. furfur (1:4) was compared to those fed M. furfur at 6 d of age. (E) The percentage of worms with body-cavity leakage fed OP and LR + M. furfur (1:4) was compared to those fed OP + M. furfur (1:4) at 10 and 13 d of age. For (C) through (E), results are shown as individual plots and means ± standard error of mean (SEM). Statistical analysis was performed using one-way ANOVA and Tukey’s multiple comparison tests. ***, P < 0.001; **, P < 0.01; *, P < 0.05; N.S., not significant. (F) Representative images of 10-d-old worms fed OP, OP + M. furfur (1:4), or LR + M. furfur (1:4) and stained with blue dye. Scale bar, 100 µm.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques: Bacteria, Comparison, Staining

Journal: bioRxiv

Article Title: Pathogenicity and intestinal barrier disruptive ability of Malassezia furfur in an alternative model host Caenorhabditis elegans is partially alleviated by Lacticaseibacillus rhamnosus

doi: 10.1101/2024.10.17.618914

Figure Lengend Snippet: Schematic representation of the pathogenicity of Malassezia furfur in Caenorhabditis elegans . (A) The protective effect of Lacticaseibacillus rhamnosus . (B) The signaling pathway.

Article Snippet: Malassezia furfur was cultured in CHROMagar Malassezia/Candida media (Kanto Chemical, Tokyo, Japan) at 30 °C for 72 h. Lactic acid bacteria were cultivated anaerobically in No. 804 medium (LR and LH), and in MRS medium ( L. plantarum; Kanto Chemical).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: Antifungal activity of MG and HL against M. furfur .

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques: Activity Assay

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: Effect of treatment with MG, HL, and ML on the time-kill curve of M. furfur .

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: SEM images of M. furfur treated with MG, HL, and ML.

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: TEM images of M. furfur treated with MG, HL, and ML.

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: PI staining images of Effect of M. furfur treated with MG, HL, and ML (x10).

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques: Staining

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: Effect of intracellular protein content of M. furfur treated with MG, HL, and ML (compared to control, ** p < 0.01; compared to the MG 3.125 mg/mL group, # p < 0.05, ## p < 0.01; compared to HL 6.25 mg/mL group, △△ p < 0.01).

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques: Control

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: Effect of ergosterol content of M. furfur treated with MG, HL, and ML (compared to control, ** p < 0.01; compared to the MG 3.125 mg/mL group, # p < 0.05, ## p < 0.01; compared to HL 6.25 mg/mL group, △△ p < 0.01).

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques: Control

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: GO enrichment analysis of differentially expressed genes in M. furfur treated with MG, HL, and ML.

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: KEGG pathway enrichment analysis of differentially expressed genes in M. furfur treated with MG, HL, and ML.

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques:

Journal: Frontiers in Microbiology

Article Title: In vitro and in vivo synergistic inhibition of Malassezia furfur targeting cell membranes by Rosa rugosa Thunb. and Coptidis Rhizoma extracts

doi: 10.3389/fmicb.2024.1456240

Figure Lengend Snippet: The fungal burden and the intensity of skin tissue inflammation in mice infected with M. furfur after the treatment of ML. (A) Flowchart of animal experiment; (B) chart of fungal burden (compared with the control, * p < 0.05, ** p < 0.01; compared with the model, # p < 0.05, ## p < 0.01; compared with the MG, △ p < 0.05, △△ p < 0.01; compared with the HL, ▽ p < 0.05, ▽▽ p < 0.01; compared with the ML Lowdose, ○ p < 0.05); (C) HE pathological sections (x10).

Article Snippet: Malassezia furfur ( M. furfur , BNCC324536) was procured from Beina biology-Henan industrial microbial strain engineering technology research center (Henan, China); ATCC Modified Dixon medium (mDixon) was acquired from Qingdao Haibo Biotechnology Co., Ltd. (Qingdao, China); Propidium Iodide (PI) fluorescent dye was sourced from Yesen Bio-tech (Shanghai, China); BCA Protein Assay Kit was obtained from Beyotime (Haimen, China).

Techniques: Infection, Control