macconkey broth  (Millipore)


Bioz Verified Symbol Millipore is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    MacConkey Broth
    Description:

    Catalog Number:
    70144
    Price:
    None
    Applications:
    A presumptive medium for the detection of the coliform group in water and milk.
    Buy from Supplier


    Structured Review

    Millipore macconkey broth
    Effect of growth medium, temperature and Fur complementation on lpf2 expression (A) Real time RT-PCR using RNA from EHECwt EDL933 strain grown in DMEM (pH 6.5) and <t>MacConkey</t> medium at 25°C and an OD 600 of 1.2, in iron rich and depleted conditions. Data were normalized to the EHECwt grown in DMEM (pH 6.5) medium in presence of iron and the variations in up- or down-regulation are related to this value. As a control for constitutive expression, the 16S RNA transcript was used. (B). Real time RT-PCR using RNA from EHECwt EDL933 and EHECΔ fur grown at 25°C and an OD 600 of 1.2 in DMEM (pH 6.5) with and without supplementation of 0.1 mM of iron, 0.15% of bile salts or both. Data were also normalized to the EHECwt grown in DMEM (pH 6.5). (C). Real time RT-PCR using RNA from EHECwt EDL933, EHECΔ fur , EHECΔ fur + fur and EHECΔ lpfA2 strains grown in MacConkey broth at 25°C and an OD 600 of 1.2, with and without iron. Data were normalized to the EHECwt grown in MacConkey medium in presence of iron. The EHEC lpfA2 mutant was used as negative control.

    https://www.bioz.com/result/macconkey broth/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    macconkey broth - by Bioz Stars, 2020-04
    99/100 stars

    Images

    1) Product Images from "Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7"

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7

    Journal: FEMS microbiology letters

    doi: 10.1111/1574-6968.12513

    Effect of growth medium, temperature and Fur complementation on lpf2 expression (A) Real time RT-PCR using RNA from EHECwt EDL933 strain grown in DMEM (pH 6.5) and MacConkey medium at 25°C and an OD 600 of 1.2, in iron rich and depleted conditions. Data were normalized to the EHECwt grown in DMEM (pH 6.5) medium in presence of iron and the variations in up- or down-regulation are related to this value. As a control for constitutive expression, the 16S RNA transcript was used. (B). Real time RT-PCR using RNA from EHECwt EDL933 and EHECΔ fur grown at 25°C and an OD 600 of 1.2 in DMEM (pH 6.5) with and without supplementation of 0.1 mM of iron, 0.15% of bile salts or both. Data were also normalized to the EHECwt grown in DMEM (pH 6.5). (C). Real time RT-PCR using RNA from EHECwt EDL933, EHECΔ fur , EHECΔ fur + fur and EHECΔ lpfA2 strains grown in MacConkey broth at 25°C and an OD 600 of 1.2, with and without iron. Data were normalized to the EHECwt grown in MacConkey medium in presence of iron. The EHEC lpfA2 mutant was used as negative control.
    Figure Legend Snippet: Effect of growth medium, temperature and Fur complementation on lpf2 expression (A) Real time RT-PCR using RNA from EHECwt EDL933 strain grown in DMEM (pH 6.5) and MacConkey medium at 25°C and an OD 600 of 1.2, in iron rich and depleted conditions. Data were normalized to the EHECwt grown in DMEM (pH 6.5) medium in presence of iron and the variations in up- or down-regulation are related to this value. As a control for constitutive expression, the 16S RNA transcript was used. (B). Real time RT-PCR using RNA from EHECwt EDL933 and EHECΔ fur grown at 25°C and an OD 600 of 1.2 in DMEM (pH 6.5) with and without supplementation of 0.1 mM of iron, 0.15% of bile salts or both. Data were also normalized to the EHECwt grown in DMEM (pH 6.5). (C). Real time RT-PCR using RNA from EHECwt EDL933, EHECΔ fur , EHECΔ fur + fur and EHECΔ lpfA2 strains grown in MacConkey broth at 25°C and an OD 600 of 1.2, with and without iron. Data were normalized to the EHECwt grown in MacConkey medium in presence of iron. The EHEC lpfA2 mutant was used as negative control.

    Techniques Used: Expressing, Quantitative RT-PCR, Mutagenesis, Negative Control

    Related Articles

    Centrifugation:

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Article Snippet: One to three colonies were used to inoculate 30 ml of DMEM or MacConkey broth in iron-rich and iron-depleted conditions (0.125 μM 2,2′-dipirydil) or DMEM (pH 6.5) supplemented with either 0.1 mM de FeCl3 , 0.15% bile salts (Sigma) or both and incubated at 37°C or 25°C ( ). .. Two volumes of RNAProtect reagent (QIAGEN) were added to 1 volume of bacterial culture prior to harvesting 1×109 cells by centrifugation at 12,000 rpm for 10 min at 4°C.

    Modification:

    Article Title: Carbapenem-Resistant Bacteria Recovered from Faeces of Dairy Cattle in the High Plains Region of the USA
    Article Snippet: Selective isolation of CRB Twenty-five grams of faeces were enriched in 225 mL of MacConkey broth (EMD Millipore, Darmstadt, Germany) containing ertapenem (0.5 mg/L; Toronto Research Chemicals, Inc., Toronto, Canada), which was then hand homogenised before incubation at 37°C for 18 to 22 hours. .. Following incubation, 10 μL was streaked to two agar plates as an initial screening method for CRB, one MacConkey agar (EMD Millipore) plate containing ertapenem (1.0 mg/L; ME) and one modified-SUPERCARB (SC) agar plate.

    Concentration Assay:

    Article Title: Carbapenem-Resistant Bacteria Recovered from Faeces of Dairy Cattle in the High Plains Region of the USA
    Article Snippet: Selective isolation of CRB Twenty-five grams of faeces were enriched in 225 mL of MacConkey broth (EMD Millipore, Darmstadt, Germany) containing ertapenem (0.5 mg/L; Toronto Research Chemicals, Inc., Toronto, Canada), which was then hand homogenised before incubation at 37°C for 18 to 22 hours. .. In addition, the concentration of ertapenem was raised from 0.25 mg/L to 0.5 mg/L, to reflect a change in interpretive criteria for ertapenem [ ].

    Isolation:

    Article Title: Carbapenem-Resistant Bacteria Recovered from Faeces of Dairy Cattle in the High Plains Region of the USA
    Article Snippet: .. Selective isolation of CRB Twenty-five grams of faeces were enriched in 225 mL of MacConkey broth (EMD Millipore, Darmstadt, Germany) containing ertapenem (0.5 mg/L; Toronto Research Chemicals, Inc., Toronto, Canada), which was then hand homogenised before incubation at 37°C for 18 to 22 hours. .. Following incubation, 10 μL was streaked to two agar plates as an initial screening method for CRB, one MacConkey agar (EMD Millipore) plate containing ertapenem (1.0 mg/L; ME) and one modified-SUPERCARB (SC) agar plate.

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Article Snippet: Paragraph title: RNA isolation and cDNA synthesis ... One to three colonies were used to inoculate 30 ml of DMEM or MacConkey broth in iron-rich and iron-depleted conditions (0.125 μM 2,2′-dipirydil) or DMEM (pH 6.5) supplemented with either 0.1 mM de FeCl3 , 0.15% bile salts (Sigma) or both and incubated at 37°C or 25°C ( ).

    Spectrophotometry:

    Article Title: Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp ( Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics
    Article Snippet: Samples (25 g) were dispensed in 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, an aliquot of 1 ml of MacConkey broth was serially diluted on buffered peptone water and inoculated onto MacConkey agar plates supplemented with ceftriaxone, meropenem, or colistin (2 µg/ml each; Sigma-Aldrich, St. Louis, MO) and incubated at 37°C for 24 h. A ceftriaxone-resistant E. coli isolate (ECCO2) was identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF). .. Next, DNA quality and quantity were checked with a NanoDrop spectrophotometer (Thermo Scientific) and Qubit 2.0 fluorometer (Life Technologies), respectively.

    Purification:

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Article Snippet: One to three colonies were used to inoculate 30 ml of DMEM or MacConkey broth in iron-rich and iron-depleted conditions (0.125 μM 2,2′-dipirydil) or DMEM (pH 6.5) supplemented with either 0.1 mM de FeCl3 , 0.15% bile salts (Sigma) or both and incubated at 37°C or 25°C ( ). .. The bacterial pellet was re-suspended in RNeasy lysis buffer and RNA purified using the High Pure RNA Isolation Kit (Roche, Mannheim) with subsequent DNase treatment (Roche, Mannheim).

    Polymerase Chain Reaction:

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. Next, antimicrobial susceptibility profiles and MIC values of polymyxin B and colistin were determined by disk diffusion ( ) and a microdilution method , respectively; mcr-1 , extended-spectrum β-lactamase (ESBL), and plasmid-mediated AmpC β-lactamase genes were screened by PCR and sequencing ( , ).

    Incubation:

    Article Title: Carbapenem-Resistant Bacteria Recovered from Faeces of Dairy Cattle in the High Plains Region of the USA
    Article Snippet: .. Selective isolation of CRB Twenty-five grams of faeces were enriched in 225 mL of MacConkey broth (EMD Millipore, Darmstadt, Germany) containing ertapenem (0.5 mg/L; Toronto Research Chemicals, Inc., Toronto, Canada), which was then hand homogenised before incubation at 37°C for 18 to 22 hours. .. Following incubation, 10 μL was streaked to two agar plates as an initial screening method for CRB, one MacConkey agar (EMD Millipore) plate containing ertapenem (1.0 mg/L; ME) and one modified-SUPERCARB (SC) agar plate.

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: .. Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. Next, antimicrobial susceptibility profiles and MIC values of polymyxin B and colistin were determined by disk diffusion ( ) and a microdilution method , respectively; mcr-1 , extended-spectrum β-lactamase (ESBL), and plasmid-mediated AmpC β-lactamase genes were screened by PCR and sequencing ( , ).

    Article Title: Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp ( Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics
    Article Snippet: .. Samples (25 g) were dispensed in 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, an aliquot of 1 ml of MacConkey broth was serially diluted on buffered peptone water and inoculated onto MacConkey agar plates supplemented with ceftriaxone, meropenem, or colistin (2 µg/ml each; Sigma-Aldrich, St. Louis, MO) and incubated at 37°C for 24 h. A ceftriaxone-resistant E. coli isolate (ECCO2) was identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF). .. DNA extraction was performed using the PureLink quick gel extraction kit (Life Technologies, Carlsbad, CA).

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Article Snippet: .. One to three colonies were used to inoculate 30 ml of DMEM or MacConkey broth in iron-rich and iron-depleted conditions (0.125 μM 2,2′-dipirydil) or DMEM (pH 6.5) supplemented with either 0.1 mM de FeCl3 , 0.15% bile salts (Sigma) or both and incubated at 37°C or 25°C ( ). .. Two volumes of RNAProtect reagent (QIAGEN) were added to 1 volume of bacterial culture prior to harvesting 1×109 cells by centrifugation at 12,000 rpm for 10 min at 4°C.

    Diffusion-based Assay:

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. Next, antimicrobial susceptibility profiles and MIC values of polymyxin B and colistin were determined by disk diffusion ( ) and a microdilution method , respectively; mcr-1 , extended-spectrum β-lactamase (ESBL), and plasmid-mediated AmpC β-lactamase genes were screened by PCR and sequencing ( , ).

    Plasmid Preparation:

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. Next, antimicrobial susceptibility profiles and MIC values of polymyxin B and colistin were determined by disk diffusion ( ) and a microdilution method , respectively; mcr-1 , extended-spectrum β-lactamase (ESBL), and plasmid-mediated AmpC β-lactamase genes were screened by PCR and sequencing ( , ).

    Mass Spectrometry:

    Article Title: Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp ( Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics
    Article Snippet: .. Samples (25 g) were dispensed in 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, an aliquot of 1 ml of MacConkey broth was serially diluted on buffered peptone water and inoculated onto MacConkey agar plates supplemented with ceftriaxone, meropenem, or colistin (2 µg/ml each; Sigma-Aldrich, St. Louis, MO) and incubated at 37°C for 24 h. A ceftriaxone-resistant E. coli isolate (ECCO2) was identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF). .. DNA extraction was performed using the PureLink quick gel extraction kit (Life Technologies, Carlsbad, CA).

    Gel Extraction:

    Article Title: Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp ( Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics
    Article Snippet: Samples (25 g) were dispensed in 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, an aliquot of 1 ml of MacConkey broth was serially diluted on buffered peptone water and inoculated onto MacConkey agar plates supplemented with ceftriaxone, meropenem, or colistin (2 µg/ml each; Sigma-Aldrich, St. Louis, MO) and incubated at 37°C for 24 h. A ceftriaxone-resistant E. coli isolate (ECCO2) was identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF). .. DNA extraction was performed using the PureLink quick gel extraction kit (Life Technologies, Carlsbad, CA).

    Sequencing:

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. Next, antimicrobial susceptibility profiles and MIC values of polymyxin B and colistin were determined by disk diffusion ( ) and a microdilution method , respectively; mcr-1 , extended-spectrum β-lactamase (ESBL), and plasmid-mediated AmpC β-lactamase genes were screened by PCR and sequencing ( , ).

    Article Title: Genome Sequencing of an Escherichia coli Sequence Type 617 Strain Isolated from Beach Ghost Shrimp ( Callichirus major) from a Heavily Polluted Ecosystem Reveals a Wider Resistome against Heavy Metals and Antibiotics
    Article Snippet: Here, we present the draft genome sequence of an MDR E. coli isolate (ECCO2) recovered from beach ghost shrimp ( Callichirus major ) in an impacted ecosystem. .. Samples (25 g) were dispensed in 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, an aliquot of 1 ml of MacConkey broth was serially diluted on buffered peptone water and inoculated onto MacConkey agar plates supplemented with ceftriaxone, meropenem, or colistin (2 µg/ml each; Sigma-Aldrich, St. Louis, MO) and incubated at 37°C for 24 h. A ceftriaxone-resistant E. coli isolate (ECCO2) was identified by matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF).

    Lysis:

    Article Title: Environmental regulation of the long polar fimbriae 2 of enterohemorrhagic Escherichia coli O157:H7
    Article Snippet: One to three colonies were used to inoculate 30 ml of DMEM or MacConkey broth in iron-rich and iron-depleted conditions (0.125 μM 2,2′-dipirydil) or DMEM (pH 6.5) supplemented with either 0.1 mM de FeCl3 , 0.15% bile salts (Sigma) or both and incubated at 37°C or 25°C ( ). .. The bacterial pellet was re-suspended in RNeasy lysis buffer and RNA purified using the High Pure RNA Isolation Kit (Roche, Mannheim) with subsequent DNase treatment (Roche, Mannheim).

    Pulsed-Field Gel:

    Article Title: Chicken Meat as a Reservoir of Colistin-Resistant Escherichia coli Strains Carrying mcr-1 Genes in South America
    Article Snippet: Samples (25 g) were dispensed in sterile plastic bags (Whirl-Pak; Nasco, WI) containing 225 ml of MacConkey broth and incubated at 37°C for 24 h. After incubation, a 1-ml aliquot of MacConkey broth was serially diluted in buffered peptone water, inoculated onto MacConkey agar plates containing colistin (2 μg/ml) (Sigma-Aldrich, St. Louis, MO), and incubated at 37°C for 24 h ( ). .. These isolates were found to be genetically unrelated by pulsed-field gel electrophoresis (PFGE) ( ) and were not related to other mcr-1 -positive E. coli isolates previously identified in food-producing animals ( ) and humans ( ) in Brazil.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore macconkey broth
    Macconkey Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/macconkey broth/product/Millipore
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    macconkey broth - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    92
    Millipore sterile macconkey enrichment broth
    Sterile Macconkey Enrichment Broth, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sterile macconkey enrichment broth/product/Millipore
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sterile macconkey enrichment broth - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    Image Search Results