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(A) Wild-type mice were administered TLR ligands, such as LPS (TLR4/MD-2), Pam3CSK4 (TLR1/TLR2), poly(I:C) (TLR3), R848 (TLR7), and CpG-B <t>(TLR9),</t> on days 1 and 2. Dot plots show hepatic macrophages in wild-type mice on day 3. (B) Histograms show staining of TLRs with antibodies against each TLR (red) and isotype-matched antibody (black).
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(A) Wild-type mice were administered TLR ligands, such as LPS (TLR4/MD-2), Pam3CSK4 (TLR1/TLR2), poly(I:C) (TLR3), R848 (TLR7), and CpG-B (TLR9), on days 1 and 2. Dot plots show hepatic macrophages in wild-type mice on day 3. (B) Histograms show staining of TLRs with antibodies against each TLR (red) and isotype-matched antibody (black).

Journal: bioRxiv

Article Title: Microbiome ssRNA as an environmental cue to activate TLR13-dependent tissue-protective programs in CD5L hi hepatic macrophages

doi: 10.1101/2023.05.11.540294

Figure Lengend Snippet: (A) Wild-type mice were administered TLR ligands, such as LPS (TLR4/MD-2), Pam3CSK4 (TLR1/TLR2), poly(I:C) (TLR3), R848 (TLR7), and CpG-B (TLR9), on days 1 and 2. Dot plots show hepatic macrophages in wild-type mice on day 3. (B) Histograms show staining of TLRs with antibodies against each TLR (red) and isotype-matched antibody (black).

Article Snippet: Phycoerythrin (PE)-conjugated mouse anti-TLR3 mAb (PaT3), PE-conjugated mouse anti-TLR7 mAb (A94B10), and PE-conjugated mouse anti-TLR9 mAb (J15A7) were purchased from BD Biosciences (Franklin Lakes, NJ, USA).

Techniques: Staining