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Hamsters received IN or IM vaccines on day 0 and then challenged on day 56 or day 174 with either SARS-CoV-2 Delta or Omicron. Samples were then taken at the indicated timepoints.

Journal: bioRxiv

Article Title: Intranasal Replicating Adenovirus type 4-SARS-CoV-2 Recombinants Induce Superior Immune Response Durability and Efficacy in Preclinical Testing Compared to Standard Intramuscular Vaccines

doi: 10.64898/2026.02.04.703921

Figure Lengend Snippet: Hamsters received IN or IM vaccines on day 0 and then challenged on day 56 or day 174 with either SARS-CoV-2 Delta or Omicron. Samples were then taken at the indicated timepoints.

Article Snippet: For surface staining cells were harvested using 0.01 M EDTA in PBS 48 h after infection and stained with mouse anti- SARS-CoV-2 spike monoclonal antibody (40592-MM117, Sino Biological, Inc., Beijing, China) (50 μL at 1.0 μg/mL final concentration) and then goat anti-mouse IgG PE-conjugated secondary antibody (BioLegend, San Diego, CA, USA) (100 μL at 2.0 μg/mL final concentration) at 37 °C for 30 minutes each.

Techniques: Vaccines