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c1q mab  (Hycult Biotech)


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    Hycult Biotech c1q mab
    C1q Mab, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c1q mab/product/Hycult Biotech
    Average 92 stars, based on 4 article reviews
    c1q mab - by Bioz Stars, 2026-03
    92/100 stars

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    Irradiated strains from the MTBC lineages L1, L2, L3, L4S (specialist), L4G (generalist), L5, L6 and H37Rv were incubated with recombinant (r) MBL ( A ), rficolin-2 ( B ), purified (p) C1q ( C ), rficolin-1 ( D ), rficolin-3 ( E ), rMASP-1 ( F ), rMASP-3 ( G ), and rCL-11 ( H ). Complement binding was measured by flow cytometry using specific antibodies and fluorophore-labelled secondary antibodies. Negative controls were processed identically but in the absence of PRM or MASPs. MFI levels indicate the binding after background subtraction and the data represent the means of at least three independent experiments ± SEM.

    Journal: bioRxiv

    Article Title: C1q in non-immune human serum has a non-redundant complement function towards clinical Mycobacterium tuberculosis complex strains

    doi: 10.1101/2024.11.01.621467

    Figure Lengend Snippet: Irradiated strains from the MTBC lineages L1, L2, L3, L4S (specialist), L4G (generalist), L5, L6 and H37Rv were incubated with recombinant (r) MBL ( A ), rficolin-2 ( B ), purified (p) C1q ( C ), rficolin-1 ( D ), rficolin-3 ( E ), rMASP-1 ( F ), rMASP-3 ( G ), and rCL-11 ( H ). Complement binding was measured by flow cytometry using specific antibodies and fluorophore-labelled secondary antibodies. Negative controls were processed identically but in the absence of PRM or MASPs. MFI levels indicate the binding after background subtraction and the data represent the means of at least three independent experiments ± SEM.

    Article Snippet: Inhibition of MBL and C1q was performed using anti-MBL-inhibitory mAb 3F8 [ ], and anti-C1q mAb clone CLB/C1q85 isotype IgG1 (MW1828, Sanquin, Amsterdam, Netherlands).

    Techniques: Irradiation, Incubation, Recombinant, Purification, Binding Assay, Flow Cytometry

    Twenty-one irradiated clinical strains covering the M. tuberculosis lineages L1, L2, L3, L4S (specialist), L4G (generalist), M. africanum lineages L5 and L6 and H37Rv were incubated with recombinant (r)MBL (A) , or purified (p)C1q (B) . Complement binding was measured by flow cytometry using specific antibodies and fluorophore-labelled secondary antibodies. Negative controls were processed identically but in the absence of rMBL and pC1q. MFI levels indicate the binding after background subtraction and the data represent the means of at least three independent experiments ± SEM.

    Journal: bioRxiv

    Article Title: C1q in non-immune human serum has a non-redundant complement function towards clinical Mycobacterium tuberculosis complex strains

    doi: 10.1101/2024.11.01.621467

    Figure Lengend Snippet: Twenty-one irradiated clinical strains covering the M. tuberculosis lineages L1, L2, L3, L4S (specialist), L4G (generalist), M. africanum lineages L5 and L6 and H37Rv were incubated with recombinant (r)MBL (A) , or purified (p)C1q (B) . Complement binding was measured by flow cytometry using specific antibodies and fluorophore-labelled secondary antibodies. Negative controls were processed identically but in the absence of rMBL and pC1q. MFI levels indicate the binding after background subtraction and the data represent the means of at least three independent experiments ± SEM.

    Article Snippet: Inhibition of MBL and C1q was performed using anti-MBL-inhibitory mAb 3F8 [ ], and anti-C1q mAb clone CLB/C1q85 isotype IgG1 (MW1828, Sanquin, Amsterdam, Netherlands).

    Techniques: Irradiation, Incubation, Recombinant, Purification, Binding Assay, Flow Cytometry