triton x 100 tbs  (Roche)


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    Structured Review

    Roche triton x 100 tbs
    Triton X 100 Tbs, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 2093 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/triton x 100 tbs/product/Roche
    Average 91 stars, based on 2093 article reviews
    Price from $9.99 to $1999.99
    triton x 100 tbs - by Bioz Stars, 2020-09
    91/100 stars

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    Centrifugation:

    Article Title: Molecular Characterization of the Host Defense Activity of the Barrier to Autointegration Factor against Vaccinia Virus ▿
    Article Snippet: .. Lysis was performed for 10 min on ice in lysis buffer (50 mM Tris [pH 7.4], 75 mM NaCl, 1 mM EDTA, 4% sucrose, and 0.5% Triton X-100) supplemented with fresh 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease and phosphatase inhibitors (Complete Mini and PhosStop tablets; Roche), after which nuclei were removed following centrifugation at 800 × g . .. For DNA binding assays, 50 μl of native dsDNA cellulose beads (Amersham) was added to the lysates and incubated overnight at 4°C with end-over-end rotation.

    Amplification:

    Article Title: Addition of a Single gp120 Glycan Confers Increased Binding to Dendritic Cell-Specific ICAM-3-Grabbing Nonintegrin and Neutralization Escape to Human Immunodeficiency Virus Type 1
    Article Snippet: .. Specific cDNAs were amplified according to the manufacturer's specifications in a 100-μl reaction mixture containing 5 μl of cDNA; 10 mM Tris-HCl (pH 8.5); 50 mM KCl; 1.5 mM MgCl2 ; 0.1% Triton X-100; 200 μM (each) dATP, dGTP, dCTP, and dTTP; 20 pmol of each primer; and 2.5 U of EXPAND high-fidelity DNA polymerase (Roche Diagnostic Corp., Indianapolis, Ind.). .. PCR primers hybridizing to the 5′- and 3′-untranslated regions of rhesus and human DC-SIGN were as follows (restriction sites for cloning are underlined): first round, DC-SIGNf1 (5′-TCT GGA CAC TGG GGG AGA GTG G-3′) and DC-SIGNb1 (5′-GGA TGG AGA GAA GGA ACT GTA G-3′); second round, DC-SIGNf2 (5′-TCGAG GGATCCGAATTC GGA GAG TGG GGT GAC ATG AGT G-3′) and DC-SIGNb2 (5′-TCGA GCGGCCGCTCTAGA GCT TAA AAG GGG GTG AAG TTC TG-3′).

    In Situ:

    Article Title: wrwyrggrywrw is a single-chain functional analog of the Holliday junction-binding homodimer, (wrwycr)2
    Article Snippet: .. The cultures were then pelleted, fixed with 4% paraformaldehyde, permeabilized using a solution of 0.1% Triton X-100, 0.1% sodium citrate, and assayed using the In Situ Cell Death Detection Kit Fluorescein (Roche) according to the manufacturer’s protocol, except that we resuspended our samples in a final volume of 25 μL of the detection solution and counterstained cells with 0.5 μM TOTO-3 (Life Technologies Inc.) for 10 minutes to monitor the presence of the chromosome. .. After treatment, cells were pelleted and resuspended in 1X PBS and quantified by flow cytometry using the blue laser (488 nm; FITC channel) for fluorescein, and the red laser (633 nm; APC channel) for TOTO-3.

    Radio Immunoprecipitation:

    Article Title: Nucling, a novel protein associated with NF-?B, regulates endotoxin-induced apoptosis in vivo
    Article Snippet: .. Briefly, whole liver cells were lysed in radioimmunoprecipitation assay (RIPA) buffer (1% sodium deoxycholate, 0.1% sodium dodecyl sulphate (SDS), 1% Triton X-100, 50 mM Tris–HCl, pH 7.4, 150 mM NaCl and 1 mM phenylmethylsulfonyl fluoride) with proteinase inhibitor cocktail (Roche Diagnostics, Tokyo, Japan) and phosphatase inhibitor (Nacalai Tesque, Kyoto, Japan). .. The protein concentrations of the extracts were estimated using a BCA kit (Pierce, Rockford, IL, USA).

    Protease Inhibitor:

    Article Title: A Novel Cell Lysis Approach Reveals That Caspase-2 Rapidly Translocates from the Nucleus to the Cytoplasm in Response to Apoptotic Stimuli
    Article Snippet: .. The cells were then washed with ice-cold isotonic saline and lysed in ice-cold lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM KCl, 0.5% Triton X-100 with Roche mini-complete protease inhibitor) by repeated vortexing at maximal setting and keeping the cells on ice for 10 min. Lysing cells with Triton X-100 at isotonic salt concentrations has been shown to be a rapid and effective way to isolate purified nuclei . .. The lysates were centrifuged for 10 min at 5,000 g, the extra-nuclear faction was transferred to a new tube and the nuclear pellets were washed with lysis buffer.

    Article Title: Identification of Domains and Residues within the ? Subunit of Eukaryotic Translation Initiation Factor 2B (eIF2B?) Required for Guanine Nucleotide Exchange Reveals a Novel Activation Function Promoted by eIF2B Complex Formation
    Article Snippet: .. Purified eIF2Bɛ proteins (200 nM), 100 nM purified eIF2B complex, or an equivalent concentration of control FLAG peptide was incubated with 20 μl (wet volume) of anti-FLAG M2 affinity resin (Eastman Kodak) with rotation for 2 h at 4°C in 100 μl of buffer A (100 mM KCl, 20 mM Tris-HCl [pH 7.5], 2 mM MgCl2 , 5 mM β-mercaptoethanol, 0.1% Triton X-100) in the presence of Complete EDTA-free protease inhibitor (Roche Diagnostics) and 10 μg of BSA. .. Beads were washed three times with 100 μl of buffer A. Purified eIF2 at concentrations of 0.625 to 40 nM (as indicated in the figure legends) was then added to the beads in 100 μl of buffer A in the presence of 10 μg of BSA and rotated for 2 h at 4°C.

    Article Title: Misfolded Polyglutamine, Polyalanine, and Superoxide Dismutase 1 Aggregate via Distinct Pathways in the Cell *
    Article Snippet: .. In essence, the cell pellets were extruded 25 times through a 27-gauge needle in an ice-cold solution of 20 m m Tris, 2 m m MgCl2 , 150 m m NaCl, 1% w/v Triton X-100, pH 8.0, supplemented with EDTA-free protease inhibitor mixture (Roche Applied Science) and 20 units/ml Benzonase. .. Total protein levels in the cell lysate were measured using a bicinchoninic acid assay with bovine serum albumin as the mass standard.

    Purification:

    Article Title: A Novel Cell Lysis Approach Reveals That Caspase-2 Rapidly Translocates from the Nucleus to the Cytoplasm in Response to Apoptotic Stimuli
    Article Snippet: .. The cells were then washed with ice-cold isotonic saline and lysed in ice-cold lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM KCl, 0.5% Triton X-100 with Roche mini-complete protease inhibitor) by repeated vortexing at maximal setting and keeping the cells on ice for 10 min. Lysing cells with Triton X-100 at isotonic salt concentrations has been shown to be a rapid and effective way to isolate purified nuclei . .. The lysates were centrifuged for 10 min at 5,000 g, the extra-nuclear faction was transferred to a new tube and the nuclear pellets were washed with lysis buffer.

    Article Title: Identification of Domains and Residues within the ? Subunit of Eukaryotic Translation Initiation Factor 2B (eIF2B?) Required for Guanine Nucleotide Exchange Reveals a Novel Activation Function Promoted by eIF2B Complex Formation
    Article Snippet: .. Purified eIF2Bɛ proteins (200 nM), 100 nM purified eIF2B complex, or an equivalent concentration of control FLAG peptide was incubated with 20 μl (wet volume) of anti-FLAG M2 affinity resin (Eastman Kodak) with rotation for 2 h at 4°C in 100 μl of buffer A (100 mM KCl, 20 mM Tris-HCl [pH 7.5], 2 mM MgCl2 , 5 mM β-mercaptoethanol, 0.1% Triton X-100) in the presence of Complete EDTA-free protease inhibitor (Roche Diagnostics) and 10 μg of BSA. .. Beads were washed three times with 100 μl of buffer A. Purified eIF2 at concentrations of 0.625 to 40 nM (as indicated in the figure legends) was then added to the beads in 100 μl of buffer A in the presence of 10 μg of BSA and rotated for 2 h at 4°C.

    Concentration Assay:

    Article Title: Identification of Domains and Residues within the ? Subunit of Eukaryotic Translation Initiation Factor 2B (eIF2B?) Required for Guanine Nucleotide Exchange Reveals a Novel Activation Function Promoted by eIF2B Complex Formation
    Article Snippet: .. Purified eIF2Bɛ proteins (200 nM), 100 nM purified eIF2B complex, or an equivalent concentration of control FLAG peptide was incubated with 20 μl (wet volume) of anti-FLAG M2 affinity resin (Eastman Kodak) with rotation for 2 h at 4°C in 100 μl of buffer A (100 mM KCl, 20 mM Tris-HCl [pH 7.5], 2 mM MgCl2 , 5 mM β-mercaptoethanol, 0.1% Triton X-100) in the presence of Complete EDTA-free protease inhibitor (Roche Diagnostics) and 10 μg of BSA. .. Beads were washed three times with 100 μl of buffer A. Purified eIF2 at concentrations of 0.625 to 40 nM (as indicated in the figure legends) was then added to the beads in 100 μl of buffer A in the presence of 10 μg of BSA and rotated for 2 h at 4°C.

    Incubation:

    Article Title: Identification of Domains and Residues within the ? Subunit of Eukaryotic Translation Initiation Factor 2B (eIF2B?) Required for Guanine Nucleotide Exchange Reveals a Novel Activation Function Promoted by eIF2B Complex Formation
    Article Snippet: .. Purified eIF2Bɛ proteins (200 nM), 100 nM purified eIF2B complex, or an equivalent concentration of control FLAG peptide was incubated with 20 μl (wet volume) of anti-FLAG M2 affinity resin (Eastman Kodak) with rotation for 2 h at 4°C in 100 μl of buffer A (100 mM KCl, 20 mM Tris-HCl [pH 7.5], 2 mM MgCl2 , 5 mM β-mercaptoethanol, 0.1% Triton X-100) in the presence of Complete EDTA-free protease inhibitor (Roche Diagnostics) and 10 μg of BSA. .. Beads were washed three times with 100 μl of buffer A. Purified eIF2 at concentrations of 0.625 to 40 nM (as indicated in the figure legends) was then added to the beads in 100 μl of buffer A in the presence of 10 μg of BSA and rotated for 2 h at 4°C.

    Staining:

    Article Title: The g5R (D250) Gene of African Swine Fever Virus Encodes a Nudix Hydrolase That Preferentially Degrades Diphosphoinositol Polyphosphates
    Article Snippet: .. At various times postinfection (7, 12, and 16 h) cells were fixed in 4% paraformaldehyde, permeabilized in 0.2% Triton X-100, and stained with rat anti-HA monoclonal antibody (1 in 600 dilution; Roche) and Alexa Fluor 568 goat anti-rat immunoglobulin G conjugate (1 in 800 dilution; Molecular Probes). .. Cells were visualized with a Leica TCS NT confocal microscope.

    Lysis:

    Article Title: A Novel Cell Lysis Approach Reveals That Caspase-2 Rapidly Translocates from the Nucleus to the Cytoplasm in Response to Apoptotic Stimuli
    Article Snippet: .. The cells were then washed with ice-cold isotonic saline and lysed in ice-cold lysis buffer (50 mM Tris-HCl (pH 7.4), 150 mM KCl, 0.5% Triton X-100 with Roche mini-complete protease inhibitor) by repeated vortexing at maximal setting and keeping the cells on ice for 10 min. Lysing cells with Triton X-100 at isotonic salt concentrations has been shown to be a rapid and effective way to isolate purified nuclei . .. The lysates were centrifuged for 10 min at 5,000 g, the extra-nuclear faction was transferred to a new tube and the nuclear pellets were washed with lysis buffer.

    Article Title: Molecular Characterization of the Host Defense Activity of the Barrier to Autointegration Factor against Vaccinia Virus ▿
    Article Snippet: .. Lysis was performed for 10 min on ice in lysis buffer (50 mM Tris [pH 7.4], 75 mM NaCl, 1 mM EDTA, 4% sucrose, and 0.5% Triton X-100) supplemented with fresh 1 mM phenylmethylsulfonyl fluoride (PMSF) and protease and phosphatase inhibitors (Complete Mini and PhosStop tablets; Roche), after which nuclei were removed following centrifugation at 800 × g . .. For DNA binding assays, 50 μl of native dsDNA cellulose beads (Amersham) was added to the lysates and incubated overnight at 4°C with end-over-end rotation.

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  • 85
    Roche mab against brutp
    Mab Against Brutp, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mab against brutp/product/Roche
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mab against brutp - by Bioz Stars, 2020-09
    85/100 stars
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