mab 8d7  (Hycult Biotech)

Journal: Human reproduction (Oxford, England)

Article Title: Effects of hepatitis B virus S protein on human sperm function.

doi: 10.1093/humrep/dep050

Figure Lengend Snippet: Figure 3 Confocal microscopy analysis showed the location of ASGP-R and HBs on the spermatozoa. Confocal laser microscopy images are repre- sentative of HBs-untreated (A) and HBs-treated (B) sperm cells by double-staining with FITC-conjugated anti-ASGP-R1 (8D7) MAb (green) and TRITC-labeled HBs MAb (red). (C) Enlarged photos from (B).

Article Snippet: After rinsing twice with PBS, they were blocked in 5% normal goat serum/PBS for 30 min, rinsed three times and then incubated with anti-HBs mouse MAb at 48C in a humid chamber for 16 h. The samples were rinsed and incubated with TRITC-conjugated goat anti-mouse IgG (Sigma-Aldrich) at room temperature for 40 min. For immunofluorescent localization of sperm asialoglycoprotein receptor (ASGP-R), the samples were incubated with FITC-conjugated anti-ASGP-R1 (8D7) mouse MAb (Santa Cruz Biotechnology, Inc., CA, USA) at room temperature for 1 h before mounting on coverslips with DABCO solution (Sigma-Aldrich).

Techniques: Confocal Microscopy, Microscopy, Double Staining, Labeling