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(A) TIME ANXA2 shRNA1, TIME ANXA2 shRNA2 or TIME ANXA2 scramble cells were treated with 10 Gy IR for the times indicated; (B-C) MCF7 ANXA2 shRNA1, MCF7 ANXA2 shRNA2 or MCF7 ANXA2 scramble cells were treated with (B) 10 Gy IR for the times indicated or (C) 1 mM H 2 O 2 for the times indicated. (A–C) 20 µg of each cell lysate was subjected to SDS-PAGE followed by western blot analysis with the antibodies indicated.

Journal: PLoS ONE

Article Title: Genotoxic Agents Promote the Nuclear Accumulation of Annexin A2: Role of Annexin A2 in Mitigating DNA Damage

doi: 10.1371/journal.pone.0050591

Figure Lengend Snippet: (A) TIME ANXA2 shRNA1, TIME ANXA2 shRNA2 or TIME ANXA2 scramble cells were treated with 10 Gy IR for the times indicated; (B-C) MCF7 ANXA2 shRNA1, MCF7 ANXA2 shRNA2 or MCF7 ANXA2 scramble cells were treated with (B) 10 Gy IR for the times indicated or (C) 1 mM H 2 O 2 for the times indicated. (A–C) 20 µg of each cell lysate was subjected to SDS-PAGE followed by western blot analysis with the antibodies indicated.

Article Snippet: The following antibodies were used for immunoprecipitation studies: annexin A2: D1/274.5 mouse monoclonal (made in house), S100A10: #610071 (BD Transduction laboratories), JunD: #sc-44 (SCBT) and Trx: sc-20146 (SCBT).

Techniques: SDS Page, Western Blot