m220  (Covaris)


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    Structured Review

    Covaris m220
    M220, supplied by Covaris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m220/product/Covaris
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m220 - by Bioz Stars, 2020-05
    90/100 stars

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    Sonication:

    Article Title: Epstein-Barr virus nuclear protein EBNA3C directly induces expression of AID and somatic mutations in B cells
    Article Snippet: .. Nuclei were resuspended in 1 ml of sonication buffer (50 mM Hepes, pH 7.8, 140 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 1 mM PMSF, 1 µg/ml aprotinin, and 1 µg/ml pepstatin A) and sonicated for 1 h using an M220 (75-W peak power and 26 duty cycle for 200 cycles/burst at 6°C set temperature; Covaris). .. Thereafter, a ChIP assay kit (17-295; EMD Millipore) was used according to the manufacturer’s protocol.

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    Covaris covaris m220
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    Covaris M220, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/covaris m220/product/Covaris
    Average 99 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    covaris m220 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    93
    Covaris m220 fragmentation protocol
    Comparison of three Illumina library construction protocols. <t>Covaris</t> <t>M220</t> was used to fragment DNA in TruSeq Nano.
    M220 Fragmentation Protocol, supplied by Covaris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m220 fragmentation protocol/product/Covaris
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m220 fragmentation protocol - by Bioz Stars, 2020-05
    93/100 stars
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    Comparison of three Illumina library construction protocols. Covaris M220 was used to fragment DNA in TruSeq Nano.

    Journal: Human immunology

    Article Title: Impact of Three Illumina Library Construction Methods on GC Bias and HLA Genotype Calling

    doi: 10.1016/j.humimm.2014.12.016

    Figure Lengend Snippet: Comparison of three Illumina library construction protocols. Covaris M220 was used to fragment DNA in TruSeq Nano.

    Article Snippet: Aiming for an insert size of 550 bp, 200 ng of pooled PCR products were sheared by sonication using Covaris M220 (Covaris, Woburn, MA) with duty factor 20%, peak incident power 50 W, and 200 cycles per burst for 60 sec. Amplicon fragments underwent further purification with Agencourt AMPure XP beads in 1.6× reaction volume.

    Techniques:

    Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Journal: Genes

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples

    doi: 10.3390/genes9010049

    Figure Lengend Snippet: Novel probe capture next generation sequencing (NGS) assay for sequencing mitochondrial (mt) and nuclear DNA. To circumvent choosing between mitochondrial and nuclear analysis of degraded or limited forensic DNA samples, a capture enrichment based library preparation method can be implemented. In this method: ( 1 ) DNA libraries are prepared by fragmenting the DNA samples using Covaris ® M220 Focused-ultrasonicator TM (Covaris). DNA fragments are ligated with dual-index adapters, and the amplified DNA libraries are size-selected using SPRIselect ® beads (Beckman Coulter Life Sciences, Indianapolis, IN, USA). ( 2 ) Shotgun libraries are enriched using a DNA probe based method of targeted capture enrichment for the whole mtgenome and eight categories of nuclear single nucleotide polymorphism SNP markers (426 SNPs). ( 3 ) The enriched samples are then sequenced on a NGS platform. ( 4 ) The nuclear SNP data is analyzed using NextGENe (SoftGenetics LLC, State College, PA, USA), while the mitochondrial data is analyzed using NextGENe, GeneMarker ® HTS (SoftGenetics LLC), and Mixemt [ 50 ].

    Article Snippet: Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp.

    Techniques: Next-Generation Sequencing, Sequencing, Amplification