m220 sonicator  (Covaris)


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    Structured Review

    Covaris m220 sonicator
    M220 Sonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m220 sonicator/product/Covaris
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m220 sonicator - by Bioz Stars, 2020-02
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
    Article Snippet: Paragraph title: RNA-Seq Analysis of Cloned Embryos and Blastocysts ... The cDNAs were then fragmented into approximately 200-bp fragments using an M220 sonicator (Covaris).

    Amplification:

    Article Title: Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
    Article Snippet: Complementary DNAs (cDNAs) were amplified using a SMARTer Ultra Low Input RNA cDNA preparation kit (Takara, 634890) according to the manufacturer's instructions. .. The cDNAs were then fragmented into approximately 200-bp fragments using an M220 sonicator (Covaris).

    Construct:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: Briefly, NimbleGen’s SeqCap EZ Choice technology was used to construct oligonucleotide probes complementary to the exons (Ensembl GRCh37) of 160 genes involved or predicted to be involved in Mendelian forms of cardiac disease ( ). .. Genomic DNA libraries were sheared by sonication on a M220 sonicator (Covaris), hybrdized to the capture probes, and libraries prepared from the captured material using the Kapa Biosystems (Wilmington, MA) Library Preparation Kit.

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry, with a 5 pM loading concentration and a 10-hour runtime.

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry with a 6 pM loading concentration and a 10-h runtime.

    Real-time Polymerase Chain Reaction:

    Article Title: A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
    Article Snippet: Chromatin were released and sheared to an average size of 500 bp using M220 sonicator (Covaris). .. The immunoprecipitated DNA fragments were quantified by qPCR.

    Formalin-fixed Paraffin-Embedded:

    Article Title: Comparative analysis of immune cells reveals a conserved regulatory lexicon
    Article Snippet: RNA-Seq libraries were prepared from 70 ng of starting RNA using the Ovation Human FFPE RNA-Seq Library System (NuGEN) or Ovation mouse RNA-Seq Library System (NuGEN), according to the manufacturer’s protocol. .. Fragmentation of the cDNA was achieved by sonication using the M220 sonicator (Covaris) with the following conditions: sonication time = 350 seconds; temp = 20°C; peak power = 50; duty factor = 20; cycles/burst = 200.

    Expressing:

    Article Title: Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
    Article Snippet: In the second round, gene expression was assessed in 2-cell embryos and blastocysts between SCNT-CROC + K and SCNT-CROC + K + M (50 2-cell embryos and 50 blastocysts per sample, repeated two times). .. The cDNAs were then fragmented into approximately 200-bp fragments using an M220 sonicator (Covaris).

    Flow Cytometry:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA). .. 101 bp single end sequencing was performed on a HiSeq 2500 sequencer in rapid run mode, with 7 samples per lane of the flow cell.

    Ligation:

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. After shearing, bands of ∼5 kb in length were used for library construction using the SMRTbell template prep kit 1.0 (PacBio) with two alterations, namely, an A-tailing step abd overhang adapter ligation, as opposed to blunt adapter ligation ( ).

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. After shearing, bands ∼5 kb long were used for library construction using the SMRTbell template prep kit v1.0 (PacBio) with two alterations, an A-tailing step as well as overhang adapter ligation, as opposed to blunt adapter ligation.

    Dissection:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: For analysis of splicing of ttna mutants, hearts and skeletal muscle (trunk) were isolated from ttna mutant and wild-type zebrafish embryos (72 hrpf) and adults using manual dissection after euthanasia. .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA).

    Infection:

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: Ocular infection by HSV-1 McKrae causes fatal encephalitis in approximately 50% of animals ( ). .. The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA).

    Sequencing:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: Paragraph title: Targeted capture-based sequencing and variant calling ... Genomic DNA libraries were sheared by sonication on a M220 sonicator (Covaris), hybrdized to the capture probes, and libraries prepared from the captured material using the Kapa Biosystems (Wilmington, MA) Library Preparation Kit.

    Article Title: Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
    Article Snippet: The cDNAs were then fragmented into approximately 200-bp fragments using an M220 sonicator (Covaris). .. The sequencing libraries were prepared using a ScriptSeq v2 kit (Illumina) according to the manufacturer's instructions.

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry, with a 5 pM loading concentration and a 10-hour runtime.

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: 101 bp paired end sequencing was performed on a HiSeq 2500 sequencer (Illumina, San Diego, CA). .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA).

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry with a 6 pM loading concentration and a 10-h runtime.

    Sonication:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: .. Genomic DNA libraries were sheared by sonication on a M220 sonicator (Covaris), hybrdized to the capture probes, and libraries prepared from the captured material using the Kapa Biosystems (Wilmington, MA) Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq 2500 sequencer with multiplexing of 24 samples per lane.

    Article Title: Comparative analysis of immune cells reveals a conserved regulatory lexicon
    Article Snippet: .. Fragmentation of the cDNA was achieved by sonication using the M220 sonicator (Covaris) with the following conditions: sonication time = 350 seconds; temp = 20°C; peak power = 50; duty factor = 20; cycles/burst = 200. .. The quality of the isolated RNA, as well as of the final libraries, was assessed using the 2100 Bioanalyzer (Agilent) and Qubit (Invitrogen).

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA). .. Sequencing libraries were prepared using the Ovation Ultralow Ultralow DR Library System (NuGEN Technologies, San Carlos, CA).

    Multiplexing:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: Genomic DNA libraries were sheared by sonication on a M220 sonicator (Covaris), hybrdized to the capture probes, and libraries prepared from the captured material using the Kapa Biosystems (Wilmington, MA) Library Preparation Kit. .. Libraries were sequenced on an Illumina HiSeq 2500 sequencer with multiplexing of 24 samples per lane.

    RNA Sequencing Assay:

    Article Title: Anti-apoptotic Regulation Contributes to the Successful Nuclear Reprogramming Using Cryopreserved Oocytes
    Article Snippet: Paragraph title: RNA-Seq Analysis of Cloned Embryos and Blastocysts ... The cDNAs were then fragmented into approximately 200-bp fragments using an M220 sonicator (Covaris).

    Article Title: Comparative analysis of immune cells reveals a conserved regulatory lexicon
    Article Snippet: RNA-Seq libraries were prepared from 70 ng of starting RNA using the Ovation Human FFPE RNA-Seq Library System (NuGEN) or Ovation mouse RNA-Seq Library System (NuGEN), according to the manufacturer’s protocol. .. Fragmentation of the cDNA was achieved by sonication using the M220 sonicator (Covaris) with the following conditions: sonication time = 350 seconds; temp = 20°C; peak power = 50; duty factor = 20; cycles/burst = 200.

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA). .. Sequencing libraries were prepared using the Ovation Ultralow Ultralow DR Library System (NuGEN Technologies, San Carlos, CA).

    Magnetic Beads:

    Article Title: Epigenetic regulation of RELN andGAD1 in the frontal cortex (FC) of autism spectrum disorder (ASD) subjects
    Article Snippet: Nuclei were isolated and chromatin was fragmented with a M220 Sonicator (Covaris, Woburn, MA). .. The immunoprecipitates were washed and collected using protein A/G coupled magnetic beads (Millipore, Billirica, MA).

    Mutagenesis:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: For analysis of splicing of ttna mutants, hearts and skeletal muscle (trunk) were isolated from ttna mutant and wild-type zebrafish embryos (72 hrpf) and adults using manual dissection after euthanasia. .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA).

    Isolation:

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The genomic DNA was isolated using the PureLink viral DNA minikit (Invitrogen, Carlsbad, CA), following the manufacturer’s protocol. .. The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA).

    Article Title: Epigenetic regulation of RELN andGAD1 in the frontal cortex (FC) of autism spectrum disorder (ASD) subjects
    Article Snippet: .. Nuclei were isolated and chromatin was fragmented with a M220 Sonicator (Covaris, Woburn, MA). .. Chromatin was immunoprecipitated using antibodies against DNMT1 (Novus Biologicals, Littleton, CO) and MECP2 (Diagenode, Denville, NJ).

    Article Title: Comparative analysis of immune cells reveals a conserved regulatory lexicon
    Article Snippet: Total RNA was isolated from frozen dendritic cell pellets using the RNeasy mini plus kit (QIAGEN). .. Fragmentation of the cDNA was achieved by sonication using the M220 sonicator (Covaris) with the following conditions: sonication time = 350 seconds; temp = 20°C; peak power = 50; duty factor = 20; cycles/burst = 200.

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: For analysis of splicing of ttna mutants, hearts and skeletal muscle (trunk) were isolated from ttna mutant and wild-type zebrafish embryos (72 hrpf) and adults using manual dissection after euthanasia. .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA).

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: The genomic DNA was isolated by using a PureLink viral DNA minikit (Invitrogen, Carlsbad, CA) following the manufacturer’s protocols. .. Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA).

    Purification:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA). .. Sequencing libraries were prepared using the Ovation Ultralow Ultralow DR Library System (NuGEN Technologies, San Carlos, CA).

    Immunoprecipitation:

    Article Title: A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
    Article Snippet: Chromatin were released and sheared to an average size of 500 bp using M220 sonicator (Covaris). .. The immunoprecipitation and DNA recovery procedures were as described ( ) (Supplementary Materials and Methods).

    Article Title: Epigenetic regulation of RELN andGAD1 in the frontal cortex (FC) of autism spectrum disorder (ASD) subjects
    Article Snippet: Nuclei were isolated and chromatin was fragmented with a M220 Sonicator (Covaris, Woburn, MA). .. Chromatin was immunoprecipitated using antibodies against DNMT1 (Novus Biologicals, Littleton, CO) and MECP2 (Diagenode, Denville, NJ).

    Chromatin Immunoprecipitation:

    Article Title: A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
    Article Snippet: Paragraph title: Chromatin immunoprecipitation ... Chromatin were released and sheared to an average size of 500 bp using M220 sonicator (Covaris).

    Article Title: Epigenetic regulation of RELN andGAD1 in the frontal cortex (FC) of autism spectrum disorder (ASD) subjects
    Article Snippet: Paragraph title: 2.4. Chromatin immunoprecipitation assays ... Nuclei were isolated and chromatin was fragmented with a M220 Sonicator (Covaris, Woburn, MA).

    Negative Control:

    Article Title: A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
    Article Snippet: Chromatin were released and sheared to an average size of 500 bp using M220 sonicator (Covaris). .. Intergenic region of S. pombe chromosome I (position 465226–465326) used as negative control.

    Selection:

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. Size selection and subsequent cleanup were performed using a combination of BluePippin (Sage Science, Beverly, MA) with a miniTUBE Red (Covaris) and AMPure PB beads (PacBio, Menlo Park, CA), respectively.

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. Size selection and subsequent cleanup were performed using a combination of BluePippin (Sage Science, Beverly, MA) with the red miniTUBE (Covaris) and AMPure PB beads (PacBio, Menlo Park, CA), respectively.

    Concentration Assay:

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain McKrae
    Article Snippet: The viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry, with a 5 pM loading concentration and a 10-hour runtime.

    Article Title: Complete Genome Sequence of Herpes Simplex Virus 1 Strain MacIntyre
    Article Snippet: Viral DNA shearing was carried out using an M220 sonicator (Covaris, Woburn, MA). .. The constructed libraries were then sequenced using PacBio long-read sequencing on a Sequel system using v3.0 chemistry with a 6 pM loading concentration and a 10-h runtime.

    Lysis:

    Article Title: A Pap1–Oxs1 signaling pathway for disulfide stress in Schizosaccharomyces pombe
    Article Snippet: Nucleic acid extraction by enzymatic lysis was conducted according to the Fission Yeast Handbook. .. Chromatin were released and sheared to an average size of 500 bp using M220 sonicator (Covaris).

    Variant Assay:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: Paragraph title: Targeted capture-based sequencing and variant calling ... Genomic DNA libraries were sheared by sonication on a M220 sonicator (Covaris), hybrdized to the capture probes, and libraries prepared from the captured material using the Kapa Biosystems (Wilmington, MA) Library Preparation Kit.

    Fluorescence In Situ Hybridization:

    Article Title: An internal promoter underlies the difference in disease severity between N- and C-terminal truncation mutations of Titin in zebrafish
    Article Snippet: Pooled hearts and skeletal muscle from 10 fish were used for subsequent library preparation. .. RNA was recovered from the aqueous phase and purified purified by column (RNA Clean and Concentrator, Zymo Research, R1016) according to manufacturer’s protocol. cDNA was prepared using the Ovation RNA-Seq System V2 (NuGEN Technologies) with sonication into 200bp fragments using an M220 sonicator (Covaris, Woburn, MA).

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