m220 focused ultrasonicators  (Covaris)


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    Name:
    M220 Focused ultrasonicator
    Description:
    designed for Next Gen Sequencing applications requiring fragment sizes between 150bp and 5kb Compact size and ease of use make the M220 Focused ultrasonicator the ideal DNA shearing solution for MiSeq and PGM users
    Catalog Number:
    500295pgd
    Price:
    None
    Size:
    33cm W 38 7cm D 27 3cm H
    Category:
    Instrument
    Quantity:
    1 0
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    Structured Review

    Covaris m220 focused ultrasonicators
    M220 Focused ultrasonicator
    designed for Next Gen Sequencing applications requiring fragment sizes between 150bp and 5kb Compact size and ease of use make the M220 Focused ultrasonicator the ideal DNA shearing solution for MiSeq and PGM users
    https://www.bioz.com/result/m220 focused ultrasonicators/product/Covaris
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m220 focused ultrasonicators - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Selection:

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples
    Article Snippet: .. Nuclear DNA Sensitivity and Size Selection Study The single source male control DNA NA24129 (Coriell Institute for Medical Research, Camden, NJ, USA) was mechanically fragmented using the Covaris® M220 Focused-ultrasonicatorTM (Covaris, Woburn, MA, USA) to an average size of 175 bp with a range from 25 bp to 250 bp. .. To test short template sample analysis, size selection was carried out at ≤75 bp using the Pippin Prep® (Sage Science, Beverly, MA, USA).

    Article Title: Applications of Probe Capture Enrichment Next Generation Sequencing for Whole Mitochondrial Genome and 426 Nuclear SNPs for Forensically Challenging Samples
    Article Snippet: .. DNA Fragmentation and Library Preparation All DNA samples, except the samples in the nuclear DNA sensitivity and size selection study, were mechanically sheared to 250 bp following the manufacturer’s guideline for the Covaris® M220 Focused-ultrasonicatorTM for the 50 µL screw capped tube. .. DNA libraries were then prepared using KAPA Hyper Prep Kit for Illumina® platforms (KAPA Biosystems, Roche® , San Francisco, CA, USA) following the manufacturer’s protocol with optimized PCR cycle numbers ( ).

    Polymerase Chain Reaction:

    Article Title: RAPTURE (RAD capture) panel facilitates analyses characterizing sea lamprey reproductive ecology and movement dynamics, et al. RAPTURE (RAD capture) panel facilitates analyses characterizing sea lamprey reproductive ecology and movement dynamics
    Article Snippet: .. RAD capture libraries were prepared using the library preparation protocol described above for the discovery libraries; however, libraries were sheared using the default protocol for 300 base pair fragments using a Covaris M220 Ultrasonicator (Covaris), and following PCR, equal amounts of each library (50 ng) were pooled before proceeding with a MyBaits hybridization capture reaction. ..

    Isolation:

    Article Title: The Core Subunit of A Chromatin-Remodeling Complex, ZmCHB101, Plays Essential Roles in Maize Growth and Development
    Article Snippet: .. Chromatin was isolated and sheared to 200–800 bp with M220 Focused-ultrasonicator (Covaris). .. Soluble chromatin was incubated with anti-H3 antibody (Abcom, ab1791) or rabbit serum overnight at 4 °C.

    Magnetic Beads:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Sequencing:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Modification:

    Article Title: Genomic Analysis Reveals Novel Diversity among the 1976 Philadelphia Legionnaires’ Disease Outbreak Isolates and Additional ST36 Strains
    Article Snippet: .. In preparation for Illumina MiSeq sequencing, 2 μg of gDNA was sheared to an average fragment length of 600 bp using a Covaris M220 ultrasonicator (Woburn, MA), cleaned and concentrated with Agencourt AMPure XP magnetic beads (cat. no. A63880, Beckman Coulter, Indianapolis, IN) as per the manufacturer’s instructions with one minor modification (80% ethanol used for both washes), and eluted in Tris-HCl, pH 8.0. .. DNA quality (260:280 and 260:230) was measured on a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE) and initial average fragment size was verified on the Agilent Tapestation 2200 (Santa Clara, CA) using the D1000 ScreenTape and reagents (cat. no. 5067–5582 and 5067–5583) with an external ladder.

    Sonication:

    Article Title: Human DNA-PK activates a STING-independent DNA sensing pathway.
    Article Snippet: .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection. .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection.

    Chromatin Immunoprecipitation:

    Article Title: Human DNA-PK activates a STING-independent DNA sensing pathway.
    Article Snippet: .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection. .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection.

    Plasmid Preparation:

    Article Title: Human DNA-PK activates a STING-independent DNA sensing pathway.
    Article Snippet: .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection. .. Detection of intracellular DNA by the cGAS-STING pathway activates a type I interferon-mediated innate immune response that protects from virus infection.

    Hybridization:

    Article Title: RAPTURE (RAD capture) panel facilitates analyses characterizing sea lamprey reproductive ecology and movement dynamics, et al. RAPTURE (RAD capture) panel facilitates analyses characterizing sea lamprey reproductive ecology and movement dynamics
    Article Snippet: .. RAD capture libraries were prepared using the library preparation protocol described above for the discovery libraries; however, libraries were sheared using the default protocol for 300 base pair fragments using a Covaris M220 Ultrasonicator (Covaris), and following PCR, equal amounts of each library (50 ng) were pooled before proceeding with a MyBaits hybridization capture reaction. ..

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  • 99
    Covaris m220 focused ultrasonicator
    M220 Focused Ultrasonicator, supplied by Covaris, used in various techniques. Bioz Stars score: 99/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m220 focused ultrasonicator/product/Covaris
    Average 99 stars, based on 52 article reviews
    Price from $9.99 to $1999.99
    m220 focused ultrasonicator - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

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