m13mp18 rfi dsdna  (New England Biolabs)


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    Name:
    M13mp18 RF I DNA
    Description:
    M13mp18 RF I DNA 10 ug
    Catalog Number:
    n4018s
    Price:
    87
    Size:
    10 ug
    Category:
    Genomic DNA
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    Structured Review

    New England Biolabs m13mp18 rfi dsdna
    M13mp18 RF I DNA
    M13mp18 RF I DNA 10 ug
    https://www.bioz.com/result/m13mp18 rfi dsdna/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m13mp18 rfi dsdna - by Bioz Stars, 2020-08
    94/100 stars

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    Related Articles

    Ligation:

    Article Title: Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus and Rhodothermus marinus DNA ligases to other thermophilic ligases
    Article Snippet: .. Our system for the analysis of the rate and fidelity of DNA ligases is centred around the sequential ligation of oligonucleotides from a complete library, using M13mp18 ssDNA as a template. .. This process of sequential ligation is directed by the 32 P-labelled primer M13P (Fig. ).

    Article Title: Optimised ligation of oligonucleotides by thermal ligases: comparison of Thermus scotoductus and Rhodothermus marinus DNA ligases to other thermophilic ligases
    Article Snippet: .. Each library was incorporated separately into the following ligation reactions in which the libraries were present at different concentrations ranging from 1.3 × 10–1 to 15.7 nmol of library (2–120 fmol of each individual oligonucleotide), 15 fmol of M13mp18 ssDNA (New England Biolabs), 60 fmol of γ-32 P-labelled M13P oligonucleotide, 60 fmol of unlabelled M13P oligonucleotide, 0.5× buffer (10 mM Tris–HCl, pH 8.3, 25 mM KCl, 5 mM MgCl2 , 0.5 mM EDTA, 0.5 mM NAD+ , 5 mM DTT, 0.25% Triton X-100; Advanced Biotechnologies Ltd), 1.3 pmol Tth DNA ligase, in a total volume of 10 µl. ..

    Generated:

    Article Title: DNA-nanostructure-assembly by sequential spotting
    Article Snippet: .. DNA preparation The DNA-construct was generated by digesting 10 μg M13mp18 RF I DNA plasmid (New England - BioLabs GmbH, 65926 Frankfurt a. M., Germany) simultaneously with the restriction enzymes PstI, Acc65I and BamHI (New England - BioLabs GmbH, 65926 Frankfurt a. M., Germany) in NEBuffer-3 at 37°C for 2 h. Then the enzymes were inactivated by heating the batch to 80°C for 20 minutes and finally cooling down slowly (1 K/min.). .. Parallel to this, hybridization of the adapter segments in Tris-Cl buffer (100 mM Tris-Cl; 600 mM NaCl; pH 7.4) took place by heating the oligonucleotides up to 90°C for 5 minutes (see figure ) and cooling down slowly (1 K/min.).

    Labeling:

    Article Title: ATP?S Disrupts Human Immunodeficiency Virus Type 1 Virion Core Integrity
    Article Snippet: .. After incubating at 37°C for 1 h, unincorporated nucleotide was removed by passing the primer through a MicroSpin S-200 HR column and the labeled primer was annealed to 0.25 μg of M13mp18 DNA (New England Biolabs) by heating to 96°C and slowly cooling to room temperature; 0.5 μg of reverse transcriptase was added to the labeled template-primer complex in 25 mM Tris-HCl (pH 8.0)-75 mM KCl-8 mM MgCl2 -2 mM dithiothreitol-100 μg of bovine serum albumin per ml-10 mM CHAPS. ..

    Incubation:

    Article Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
    Article Snippet: .. M13mp18 was then added to the 30 L reaction mixture and incubated for an additional 45 minutes. .. A fraction of the reaction was taken out every 5 minutes, quenched in 6X purple gel loading dye (New England Biolabs Inc.), and subsequently analyzed in 1% agarose gel (Fisher Scientific) .

    Sequencing:

    Article Title: The Initial Response of a Eukaryotic Replisome to DNA Damage
    Article Snippet: .. Primase assay Primed template was prepared by annealing oligonucleotide JY180 (500 nM) (sequence: ) to M13mp18 ssDNA (50 nM) (New England Biolabs) in 10 mM Tris-Cl pH 7.6, 5 mM EDTA and 100 mM NaCl. .. Unannealed oligonucleotide was removed with an S400 column (GE Healthcare).

    Plasmid Preparation:

    Article Title: DNA-nanostructure-assembly by sequential spotting
    Article Snippet: .. DNA preparation The DNA-construct was generated by digesting 10 μg M13mp18 RF I DNA plasmid (New England - BioLabs GmbH, 65926 Frankfurt a. M., Germany) simultaneously with the restriction enzymes PstI, Acc65I and BamHI (New England - BioLabs GmbH, 65926 Frankfurt a. M., Germany) in NEBuffer-3 at 37°C for 2 h. Then the enzymes were inactivated by heating the batch to 80°C for 20 minutes and finally cooling down slowly (1 K/min.). .. Parallel to this, hybridization of the adapter segments in Tris-Cl buffer (100 mM Tris-Cl; 600 mM NaCl; pH 7.4) took place by heating the oligonucleotides up to 90°C for 5 minutes (see figure ) and cooling down slowly (1 K/min.).

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  • 94
    New England Biolabs m13mp18 rfi dsdna
    M13mp18 Rfi Dsdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m13mp18 rfi dsdna/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m13mp18 rfi dsdna - by Bioz Stars, 2020-08
    94/100 stars
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