Structured Review

Thermo Fisher lu6
Schematic map of the cloned region of Bacillus sp. LU11 genomic DNA. The cloned 5196 bp Cla I fragment and part of the inverse PCR product are combined together. The arrows indicate open reading frames (ORFs) which were found in the cloned region. Designations for genetic markers are: bspLU11IIIMa , gene encoding M. Bsp LU11IIIa m 6 A MTase; bspLU11IIIMb , gene encoding M. Bsp LU11IIIb m 5 C MTase; bspLU11IIIR , gene encoding R. Bsp LU11III ENase. Annealing sites for oligonucleotides 39, 40, 257, LU1, LU3, <t>LU6,</t> LU9 and LU10 are shown by small arrows.
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Images

1) Product Images from "Characterization of the type IV restriction modification system BspLU11III from Bacillus sp. LU11"

Article Title: Characterization of the type IV restriction modification system BspLU11III from Bacillus sp. LU11

Journal: Nucleic Acids Research

doi:

Schematic map of the cloned region of Bacillus sp. LU11 genomic DNA. The cloned 5196 bp Cla I fragment and part of the inverse PCR product are combined together. The arrows indicate open reading frames (ORFs) which were found in the cloned region. Designations for genetic markers are: bspLU11IIIMa , gene encoding M. Bsp LU11IIIa m 6 A MTase; bspLU11IIIMb , gene encoding M. Bsp LU11IIIb m 5 C MTase; bspLU11IIIR , gene encoding R. Bsp LU11III ENase. Annealing sites for oligonucleotides 39, 40, 257, LU1, LU3, LU6, LU9 and LU10 are shown by small arrows.
Figure Legend Snippet: Schematic map of the cloned region of Bacillus sp. LU11 genomic DNA. The cloned 5196 bp Cla I fragment and part of the inverse PCR product are combined together. The arrows indicate open reading frames (ORFs) which were found in the cloned region. Designations for genetic markers are: bspLU11IIIMa , gene encoding M. Bsp LU11IIIa m 6 A MTase; bspLU11IIIMb , gene encoding M. Bsp LU11IIIb m 5 C MTase; bspLU11IIIR , gene encoding R. Bsp LU11III ENase. Annealing sites for oligonucleotides 39, 40, 257, LU1, LU3, LU6, LU9 and LU10 are shown by small arrows.

Techniques Used: Clone Assay, Inverse PCR

Related Articles

Sequencing:

Article Title: Characterization of a Laboratory-Derived, High-Level Ampicillin-Resistant Salmonella enterica Serovar Typhimurium Strain That Caused Meningitis in an Infant
Article Snippet: .. Since the preliminary sequence data obtained by using the first primer suggested that the plasmid was likely a commercial cloning vector, we further used the M13/pUC sequencing primers (forward and reverse) (MBI Fermentas Inc.) to amplify and sequence the multiple cloning site region of the plasmid. .. Sequencing was performed on an ABI 373A automatic sequencer (Perkin-Elmer, Applied Biosystems).

Clone Assay:

Article Title: Mechanism of RNA Recombination in Carmo- and Tombusviruses: Evidence for Template Switching by the RNA-Dependent RNA Polymerase In Vitro †
Article Snippet: .. A representative number of independent clones were sequenced using the M13/pUC19 reverse primer (Gibco BRL). .. First, standard TCV RdRp reactions (including [32 P]UTP, see above) were performed with AU1/art and Mot1/pr templates separately, followed by removal of the free nucleotides by passing the reaction mixtures through Micro Bio-Spin columns (Bio-Rad).

Article Title: Characterization of a Laboratory-Derived, High-Level Ampicillin-Resistant Salmonella enterica Serovar Typhimurium Strain That Caused Meningitis in an Infant
Article Snippet: .. Since the preliminary sequence data obtained by using the first primer suggested that the plasmid was likely a commercial cloning vector, we further used the M13/pUC sequencing primers (forward and reverse) (MBI Fermentas Inc.) to amplify and sequence the multiple cloning site region of the plasmid. .. Sequencing was performed on an ABI 373A automatic sequencer (Perkin-Elmer, Applied Biosystems).

Plasmid Preparation:

Article Title: Characterization of a Laboratory-Derived, High-Level Ampicillin-Resistant Salmonella enterica Serovar Typhimurium Strain That Caused Meningitis in an Infant
Article Snippet: .. Since the preliminary sequence data obtained by using the first primer suggested that the plasmid was likely a commercial cloning vector, we further used the M13/pUC sequencing primers (forward and reverse) (MBI Fermentas Inc.) to amplify and sequence the multiple cloning site region of the plasmid. .. Sequencing was performed on an ABI 373A automatic sequencer (Perkin-Elmer, Applied Biosystems).