m v dnase i  (Thermo Fisher)


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    Structured Review

    Thermo Fisher m v dnase i
    M V Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m v dnase i/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m v dnase i - by Bioz Stars, 2020-04
    93/100 stars

    Related Products / Commonly Used Together

    ebis isolation
    collagenase

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    Related Articles

    Centrifugation:

    Article Title: Monocyte-derived macrophages expand the murine stress erythropoietic niche during the recovery from anemia
    Article Snippet: The isolation of splenic EBIs isolation was done as previously described., In brief, spleens were minced and digested in 0.075% (m/v) Collagenase IV (Gibco 17104019) and 0.004% (m/v) DNase I (Invitrogen DN25) in RPMI 1640 for 30 minutes at 37°C. .. The suspension was gently passed through an 18-gauge needle several times, and cells were washed by centrifugation.

    Isolation:

    Article Title: Monocyte-derived macrophages expand the murine stress erythropoietic niche during the recovery from anemia
    Article Snippet: .. The isolation of splenic EBIs isolation was done as previously described., In brief, spleens were minced and digested in 0.075% (m/v) Collagenase IV (Gibco 17104019) and 0.004% (m/v) DNase I (Invitrogen DN25) in RPMI 1640 for 30 minutes at 37°C. .. The suspension was gently passed through an 18-gauge needle several times, and cells were washed by centrifugation.

    Sedimentation:

    Article Title: Monocyte-derived macrophages expand the murine stress erythropoietic niche during the recovery from anemia
    Article Snippet: The isolation of splenic EBIs isolation was done as previously described., In brief, spleens were minced and digested in 0.075% (m/v) Collagenase IV (Gibco 17104019) and 0.004% (m/v) DNase I (Invitrogen DN25) in RPMI 1640 for 30 minutes at 37°C. .. Cell pellets were resuspended with 1 mL RPMI 1640 containing 0.004% DNaseI and layered on top of 30% (vol/vol) fetal bovine serum in Iscove modified Dulbecco medium followed by gravity sedimentation for 45 minutes at room temperature.

    Modification:

    Article Title: Monocyte-derived macrophages expand the murine stress erythropoietic niche during the recovery from anemia
    Article Snippet: The isolation of splenic EBIs isolation was done as previously described., In brief, spleens were minced and digested in 0.075% (m/v) Collagenase IV (Gibco 17104019) and 0.004% (m/v) DNase I (Invitrogen DN25) in RPMI 1640 for 30 minutes at 37°C. .. Cell pellets were resuspended with 1 mL RPMI 1640 containing 0.004% DNaseI and layered on top of 30% (vol/vol) fetal bovine serum in Iscove modified Dulbecco medium followed by gravity sedimentation for 45 minutes at room temperature.

    Gradient Centrifugation:

    Article Title: Monocyte-derived macrophages expand the murine stress erythropoietic niche during the recovery from anemia
    Article Snippet: The isolation of splenic EBIs isolation was done as previously described., In brief, spleens were minced and digested in 0.075% (m/v) Collagenase IV (Gibco 17104019) and 0.004% (m/v) DNase I (Invitrogen DN25) in RPMI 1640 for 30 minutes at 37°C. .. The supernatant was carefully removed, and the pellet was processed with Percoll (Sigma) gradient centrifugation (50% [vol/vol]/100% [vol/vol]) at 400 g for 20 minutes.

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  • 99
    Thermo Fisher dnase i
    Dnase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dnase i/product/Thermo Fisher
    Average 99 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    dnase i - by Bioz Stars, 2020-04
    99/100 stars
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