Structured Review

Thermo Fisher m tris
Emission spectra of fluorescently labeled ZPIs and their complexes with protein Z. A , emission spectra of 100 n m <t>NBD-K239C</t> ZPI in pH 7. 1, I 0.15 <t>Tris</t> buffer at 25 °C were taken with excitation at 480 nm in the absence and presence of 300 n m PZ.
M Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris/product/Thermo Fisher
Average 99 stars, based on 23 article reviews
Price from $9.99 to $1999.99
m tris - by Bioz Stars, 2020-09
99/100 stars

Images

1) Product Images from "Thermodynamic and Kinetic Characterization of the Protein Z-dependent Protease Inhibitor (ZPI)-Protein Z Interaction Reveals an Unexpected Role for ZPI Lys-239 *"

Article Title: Thermodynamic and Kinetic Characterization of the Protein Z-dependent Protease Inhibitor (ZPI)-Protein Z Interaction Reveals an Unexpected Role for ZPI Lys-239 *

Journal: The Journal of Biological Chemistry

doi: 10.1074/jbc.M114.633479

Emission spectra of fluorescently labeled ZPIs and their complexes with protein Z. A , emission spectra of 100 n m NBD-K239C ZPI in pH 7. 1, I 0.15 Tris buffer at 25 °C were taken with excitation at 480 nm in the absence and presence of 300 n m PZ.
Figure Legend Snippet: Emission spectra of fluorescently labeled ZPIs and their complexes with protein Z. A , emission spectra of 100 n m NBD-K239C ZPI in pH 7. 1, I 0.15 Tris buffer at 25 °C were taken with excitation at 480 nm in the absence and presence of 300 n m PZ.

Techniques Used: Labeling

Equilibrium binding titrations of the NBD-ZPI-PZ interaction at varying ionic strengths. Fluorescence titrations of NBD-ZPI (25 n m ) in pH 7. 1 Tris buffer containing 0.1 (●), 0.175 (○), 0.25 (▴), 0.35 (▵), and 0.5 (■)
Figure Legend Snippet: Equilibrium binding titrations of the NBD-ZPI-PZ interaction at varying ionic strengths. Fluorescence titrations of NBD-ZPI (25 n m ) in pH 7. 1 Tris buffer containing 0.1 (●), 0.175 (○), 0.25 (▴), 0.35 (▵), and 0.5 (■)

Techniques Used: Binding Assay, Fluorescence

Calcium ion effects on the ZPI-PZ interaction. A , equilibrium binding titrations of 25 n m NBD-ZPI with PZ in pH 7. 1, I 0.15 Tris buffer at 25 °C (●) without or with the addition of 15 m m NaCl (○) or 5 m m CaCl 2 (▴). Observed
Figure Legend Snippet: Calcium ion effects on the ZPI-PZ interaction. A , equilibrium binding titrations of 25 n m NBD-ZPI with PZ in pH 7. 1, I 0.15 Tris buffer at 25 °C (●) without or with the addition of 15 m m NaCl (○) or 5 m m CaCl 2 (▴). Observed

Techniques Used: Binding Assay

Related Articles

Irradiation:

Article Title: Intrinsic Buffer Hydroxyl Radical Dosimetry Using Tris(Hydroxymethyl)Aminomethane
Article Snippet: .. Upon comparing the UV absorbance spectra of Tris (obtained with a NanoDrop spectrometer) both with and without laser irradiation, we observed that, while Tris buffer is inherently absorbent in the short wavelength region of the ultraviolet spectrum, this absorbance changes little after oxidation by FPOP. ..

Chromatography:

Article Title: Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes
Article Snippet: .. Briefly, 3 μg recombinant Arabidopsis thaliana RGP1 and 1 mM UDP-Araf were added to 20 mM Tris, 5 mM MnCl2 , 250 mM NaCl, pH 6.8, and incubated at 30°C for 1 h. The product was detected with high-performance anion-exchange chromatography using a Dionex Ultimate 3000 with CarboPac PA20 column (3 × 150 mm; Dionex; Sunnyvale, USA). .. Nucleotide sugars were separated with 0 to 2.1 min, 50 mM ammonium formate, isocratic; 2.1 to 40 min, 50 mM to 1 M ammonium formate, linear, 40 to 45 min, 1 M to 50 mM ammonium formate, linear, 45 to 55 min, 50 mM ammonium formate, isocratic, all at a flow rate of 0.5 mL/min.

Protease Inhibitor:

Article Title: The conformation of the histone H3 tail inhibits association of the BPTF PHD finger with the nucleosome
Article Snippet: .. To purify H3(1–44) constructs, cells were lysed in 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM benzamidine, 2 mM EDTA, 0.5% Triton X-100, 0.5 mg mL−1 lysozyme with DnaseI and Pierce Protease Inhibitor Tablets (ThermoFisher Scientific) using an Avestin EmulsiFlex. .. The soluble portion of the lysate was purified using cation exchange resin and size exclusion chromatography (Superdex 30, GE Healthcare Life Sciences) in 50 mM KPi pH 7, 50 mM KCl, 0.5 mM EDTA.

Construct:

Article Title: The conformation of the histone H3 tail inhibits association of the BPTF PHD finger with the nucleosome
Article Snippet: .. To purify H3(1–44) constructs, cells were lysed in 50 mM Tris pH 7.5, 100 mM NaCl, 1 mM benzamidine, 2 mM EDTA, 0.5% Triton X-100, 0.5 mg mL−1 lysozyme with DnaseI and Pierce Protease Inhibitor Tablets (ThermoFisher Scientific) using an Avestin EmulsiFlex. .. The soluble portion of the lysate was purified using cation exchange resin and size exclusion chromatography (Superdex 30, GE Healthcare Life Sciences) in 50 mM KPi pH 7, 50 mM KCl, 0.5 mM EDTA.

Purification:

Article Title: Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers
Article Snippet: .. Tetrahemoglobin was produced from SGE2812 by treating the purified deoxygenated dihemoglobin at 150 mg/mL in 50 mM Tris pH 8.0 with a two-fold excess of bismaleimidohexane (Pierce, Inc., Salinas, CA, USA) for 2 h on ice. .. The crosslinked tetrahemoglobin was purified as described above using S-200 and S-300 SEC, followed by Q-Sepharose to remove endotoxins prior to formulation for preclinical testing.

Article Title: Control of VWF A2 domain stability and ADAMTS13 access to the scissile bond of full-length VWF
Article Snippet: .. Purified VWF A2 domain variants were dialyzed into 20 mM Tris (pH 7.8), 50 mM NaCl, and protein quantified by bicinchoninic acid assay (Pierce; Thermo Scientific). .. Samples contained 1.5 µg of purified VWF A2 domain, 5× SyproOrange dye (Sigma-Aldrich), 1 mM EDTA or 5mM CaCl2 in 20 mM Tris (pH 7.8), 50 mM NaCl in a total volume of 60 µL.

Acid Assay:

Article Title: Control of VWF A2 domain stability and ADAMTS13 access to the scissile bond of full-length VWF
Article Snippet: .. Purified VWF A2 domain variants were dialyzed into 20 mM Tris (pH 7.8), 50 mM NaCl, and protein quantified by bicinchoninic acid assay (Pierce; Thermo Scientific). .. Samples contained 1.5 µg of purified VWF A2 domain, 5× SyproOrange dye (Sigma-Aldrich), 1 mM EDTA or 5mM CaCl2 in 20 mM Tris (pH 7.8), 50 mM NaCl in a total volume of 60 µL.

Incubation:

Article Title: Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes
Article Snippet: .. Briefly, 3 μg recombinant Arabidopsis thaliana RGP1 and 1 mM UDP-Araf were added to 20 mM Tris, 5 mM MnCl2 , 250 mM NaCl, pH 6.8, and incubated at 30°C for 1 h. The product was detected with high-performance anion-exchange chromatography using a Dionex Ultimate 3000 with CarboPac PA20 column (3 × 150 mm; Dionex; Sunnyvale, USA). .. Nucleotide sugars were separated with 0 to 2.1 min, 50 mM ammonium formate, isocratic; 2.1 to 40 min, 50 mM to 1 M ammonium formate, linear, 40 to 45 min, 1 M to 50 mM ammonium formate, linear, 45 to 55 min, 50 mM ammonium formate, isocratic, all at a flow rate of 0.5 mL/min.

Produced:

Article Title: Monodisperse 130 kDa and 260 kDa Recombinant Human Hemoglobin Polymers as Scaffolds for Protein Engineering of Hemoglobin-Based Oxygen Carriers
Article Snippet: .. Tetrahemoglobin was produced from SGE2812 by treating the purified deoxygenated dihemoglobin at 150 mg/mL in 50 mM Tris pH 8.0 with a two-fold excess of bismaleimidohexane (Pierce, Inc., Salinas, CA, USA) for 2 h on ice. .. The crosslinked tetrahemoglobin was purified as described above using S-200 and S-300 SEC, followed by Q-Sepharose to remove endotoxins prior to formulation for preclinical testing.

Recombinant:

Article Title: Plant cell wall glycosyltransferases: High-throughput recombinant expression screening and general requirements for these challenging enzymes
Article Snippet: .. Briefly, 3 μg recombinant Arabidopsis thaliana RGP1 and 1 mM UDP-Araf were added to 20 mM Tris, 5 mM MnCl2 , 250 mM NaCl, pH 6.8, and incubated at 30°C for 1 h. The product was detected with high-performance anion-exchange chromatography using a Dionex Ultimate 3000 with CarboPac PA20 column (3 × 150 mm; Dionex; Sunnyvale, USA). .. Nucleotide sugars were separated with 0 to 2.1 min, 50 mM ammonium formate, isocratic; 2.1 to 40 min, 50 mM to 1 M ammonium formate, linear, 40 to 45 min, 1 M to 50 mM ammonium formate, linear, 45 to 55 min, 50 mM ammonium formate, isocratic, all at a flow rate of 0.5 mL/min.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Thermo Fisher tris 1 m ph 8 0
    Tris 1 M Ph 8 0, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris 1 m ph 8 0/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tris 1 m ph 8 0 - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    91
    Thermo Fisher m tris
    M Tris, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris/product/Thermo Fisher
    Average 91 stars, based on 185 article reviews
    Price from $9.99 to $1999.99
    m tris - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    92
    Thermo Fisher m tris glycine
    M Tris Glycine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris glycine/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m tris glycine - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results