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Carl Roth GmbH m tris
M Tris, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris/product/Carl Roth GmbH
Average 92 stars, based on 1 article reviews
Price from $9.99 to $1999.99
m tris - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Lysis:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Stripping Membranes:

Article Title: β-Secretase BACE1 Regulates Hippocampal and Reconstituted M-Currents in a β-Subunit-Like Fashion
Article Snippet: .. Membranes were reprobed after stripping: 6 m guanidine-HCl (C. Roth), 20 m m Tris, 0.2% Triton X-100, pH 7.5, and 0.8% β-mercaptoethanol (C. Roth). .. Western blot signals were quantified using the ImageJ software (Wayne Rasband, Research Services Branch, National Institute of Mental Health, Bethesda, MD).

Blocking Assay:

Article Title: Snapshots of Protein Dynamics and Post-translational Modifications In One Experiment--?-Catenin and Its Functions *
Article Snippet: .. Twenty-five or 50 μg protein extracts were adjusted to a volume of 40 μl using lysis buffer containing 150 m m NaCl (Merck KGaA), 50 m m Tris pH 7.5 (CARL ROTH GmbH, Karlsruhe, Germany), and 1% Triton X-100 (Sigma-Aldrich, St. Louis, MO), whereas the microspheres were diluted in assay buffer (ELISA blocking reagent, Roche Applied Science). .. Protein extracts were incubated with 20 μl of microspheres (2000 per set/well), overnight at 4 °C in a 96-well plate (nonbinding surface, Corning Inc., New York, NY).

Hybridization:

Article Title: Deletion of Interleukin-6 Signal Transducer gp130 in Small Sensory Neurons Attenuates Mechanonociception and Down-Regulates TRPA1 Expression
Article Snippet: .. Hybridization was performed overnight at 68°C in a humidified chamber. cRNA probes were diluted 1:50 in hybridization buffer containing 12.6 m m Tris (Roth), pH 7.5, 185 m m NaCl (Sigma), 10 m m NaH2 PO4 (Merck), 5 m m EDTA (Sigma), 50% deionized formamide (Sigma), 10% dextrane sulfate (Sigma), 1 mg/ml yeast RNA (Roche), and 0.5× Denhardt's (Sigma) in DEPC-treated H2 O. .. Sections were then washed two times for 30 min at 68°C in 1× SSC (Sigma), pH 7.0, 25% formamide, 0.1% Tween 20 (Sigma), twice for 30 min and once for 1 h at room temperature in 100 m m maleic acid (Sigma), 150 m m NaCl (Sigma), and 0.1% Tween 20 (Sigma), pH 7.5 (MABT).

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    Carl Roth GmbH m tris hcl
    M Tris Hcl, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Carl Roth GmbH
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    91/100 stars
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