Structured Review

BioShop m tris
M Tris, supplied by BioShop, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris/product/BioShop
Average 91 stars, based on 2 article reviews
Price from $9.99 to $1999.99
m tris - by Bioz Stars, 2020-09
91/100 stars

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Related Articles

Isolation:

Article Title: Genetic Ablation of Calcium-independent Phospholipase A2γ Induces Glomerular Injury in Mice *
Article Snippet: .. Lysates of isolated glomeruli ( ) were prepared in buffer containing 1% Triton X-100, 125 m m NaCl, 10 m m Tris (pH 7.4), 1 m m EGTA, 2 m m Na3 VO4 , 10 m m sodium pyrophosphate, 25 m m NaF, and protease inhibitor mixture (Bioshop, Burlington, Canada). .. Equal amounts of lysate proteins were dissolved in Laemmli buffer and were subjected to SDS-PAGE under reducing conditions.

Incubation:

Article Title: Time-resolved Phosphoproteome Analysis of Paradoxical RAF Activation Reveals Novel Targets of ERK *
Article Snippet: .. All samples were diluted five times with 20 m m Tris (Bioshop Burlington, Ontario, Canada), 1 m m CaCl, pH 8, and mixed with trypsin (Sigma-Aldrich) (protein/trypsin 50:1 w/w) and incubated for 12 h at 37 °C. ..

Article Title: Dynamic Phosphoproteomics Uncovers Signaling Pathways Modulated by Anti-oncogenic Sphingolipid Analogs
Article Snippet: .. All samples were diluted 5 times with 20 m m TRIS (Bioshop Burlington, ON, Canada), 1 m m CaCl2 , pH 8 and mixed with 2 μg/μl trypsin (Sigma-Aldrich) (protein: trypsin 50:1 w:w) and incubated for 12 h at 37 °C. .. Desalting was performed on 60 mg solid phase extraction (SPE) reverse phase cartridges (Oasis HLB 3cc cartridge, 60 mg, 30 μm particle size, Waters Mississauga, ON, Canada) previously conditioned with 3 ml methanol, SPE Buffer (50% acetonitrile, ACN, 1% formic acid, FA) and finally with 1% aqueous FA.

Protease Inhibitor:

Article Title: Genetic Ablation of Calcium-independent Phospholipase A2γ Induces Glomerular Injury in Mice *
Article Snippet: .. Lysates of isolated glomeruli ( ) were prepared in buffer containing 1% Triton X-100, 125 m m NaCl, 10 m m Tris (pH 7.4), 1 m m EGTA, 2 m m Na3 VO4 , 10 m m sodium pyrophosphate, 25 m m NaF, and protease inhibitor mixture (Bioshop, Burlington, Canada). .. Equal amounts of lysate proteins were dissolved in Laemmli buffer and were subjected to SDS-PAGE under reducing conditions.

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    BioShop m tris hcl
    M Tris Hcl, supplied by BioShop, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/BioShop
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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