Structured Review

Valiant m tris hcl
M Tris Hcl, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris hcl/product/Valiant
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
m tris hcl - by Bioz Stars, 2020-09
92/100 stars

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Related Articles

Protease Inhibitor:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Real-time Polymerase Chain Reaction:

Article Title: Identification and Characterization of the ARIADNE Gene Family in Arabidopsis. A Group of Putative E3 Ligases 1
Article Snippet: .. The cDNA reaction was diluted 1:10 with water, and 1 μL of the diluted cDNA (corresponding to 17 ng total RNA) was used as template for real-time PCR analysis with the Rotor-Gene 2000 (Corbett Research, Sydney) and our homemade SYBR green-I reaction mixture containing 1:20,000 diluted SYBR green-I (Roche Diagnostics), 200 μ m dNTPs, 10 m m Tris-HCl, pH 8.5, 50 m m KCl, 2 m m MgCl2 , 0.15% (v/v) Triton X-100, 5 pmol of each primer, and 0.5 units of Taq DNA polymerase (Qbiogene-Appligene, Heidelberg). ..

Concentration Assay:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Incubation:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Lysis:

Article Title: Exosomes Secreted by Apoptosis-Resistant Acute Myeloid Leukemia (AML) Blasts Harbor Regulatory Network Proteins Potentially Involved in Antagonism of Apoptosis *
Article Snippet: .. Cells were resuspended in serum-free IMDM (lacking phenol red) at a concentration of 2 × 106 /ml and incubated at 37 °C in 5% CO2 for 18 h. Subsequently, 5 × 106 cells were lysed in 200 μl of lysis buffer, containing 150 m m NaCl (Merck, Darmstadt, Germany) 10 m m Tris/HCl, pH 7.5 (MP biomedicals, Santa Ana, CA), 5 m m EDTA (Merck), 1% Triton X-100 (Sigma-Aldrich) with the addition of protease inhibitor mixture (Roche, Basel, Switzerland). ..

Binding Assay:

Article Title: The 2-Cys Peroxiredoxin Alkyl Hydroperoxide Reductase C Binds Heme and Participates in Its Intracellular Availability in Streptococcus agalactiae *
Article Snippet: .. Cells were pelleted at 3,500 × g for 10 min and then resuspended in 50 m m Tris-HCl, pH 8.0, 300 m m NaCl, containing 20 m m imidazole (binding buffer), and disrupted with glass beads (Fastprep, MP Biomedicals). .. Cell debris was removed by centrifugation at 18,000 × g for 15 min at 4 °C.