Structured Review

Nacalai m tris hcl
M Tris Hcl, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/m tris hcl/product/Nacalai
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
m tris hcl - by Bioz Stars, 2020-09
92/100 stars

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High Performance Liquid Chromatography:

Article Title: Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine
Article Snippet: .. The precipitate was dissolved in 1.0 ml 50 m M Tris–HCl pH 7.0 containing 0.1%( v / v ) 2-­mercaptoethanol and the enzyme solution was applied onto a HPLC gel-filtration column (5-diol-300II; Nacalai Tesque, Kyoto, Japan) equilibrated with buffer B as an additional purification step. .. The purified enzyme was dialyzed against 10 m M HEPES pH 7.0 containing 0.1%( v / v ) 2-­mercaptoethanol and concentrated to 14 mg ml−1 with an Amicon Ultra-4 30 kDa Ultracel (Millipore, Billerica, Massachusetts, USA) for crystallization.

In Vitro:

Article Title: Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel *
Article Snippet: .. At 14 days in vitro hippocampal neurons were washed with PBS, harvested, and treated like CHO cells except that for solubilization of neurons radioimmune precipitation assay buffer was used (1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS in 50 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m EDTA) supplemented with 1/100 volume of protease inhibitor mixture (Nacalai Tesque) and 1 m m phenylmethylsulfonyl fluoride. .. For the detection of native Kv12.2, crude membrane fractions were prepared from freshly dissected whole brains of mice of different ages (embryonic 17 day, postnatal day 5 (PD5), P13, or adult (P210)).

Protease Inhibitor:

Article Title: The Lon protease-like domain in the bacterial RecA paralog RadA is required for DNA binding and repair
Article Snippet: .. The cells were lysed by ultrasonication in a buffer containing 50 m m Tris-HCl (pH 7.5), 500 m m NaCl, 10 m m EDTA, and 10× protease inhibitor cocktail (Nacalai Tesque). .. The cell debris was removed by centrifugation.

Article Title: Brain-specific Phgdh Deletion Reveals a Pivotal Role for l-Serine Biosynthesis in Controlling the Level of d-Serine, an N-methyl-d-aspartate Receptor Co-agonist, in Adult Brain *
Article Snippet: .. The cerebral cortex and hippocampus were dissected from adult brain of Floxed and CKO mice (male, 7–11 weeks old) and were then homogenized in a buffer containing 10 m m Tris-HCl (pH 8.0), 8 m urea, 4% CHAPS, protease inhibitor mixture (Nacalai Tesque), and phosphatase inhibitor mixture (Nacalai Tesque). .. Homogenates were centrifuged at 20,000 × g for 10 min to obtain total protein extracts, and concentrations were determined using a Protein Assay Bicinchoninate kit (Nacalai Tesque).

Article Title: A Post Genomic Characterization of Arabidopsis Ferredoxins 1
Article Snippet: .. Proteins were extracted into 50 m m Tris-HCl, pH 7.5, 50 m m NaCl, 1 m m MgCl2 , 1 m m EDTA, 5% (w/v) polyclar A, 5 m m phenylmethylsulfonyl fluoride, and 1× protease inhibitor cocktail (Nacalai tesque, Kyoto). ..

Article Title: Triple N-Glycosylation in the Long S5-P Loop Regulates the Activation and Trafficking of the Kv12.2 Potassium Channel *
Article Snippet: .. At 14 days in vitro hippocampal neurons were washed with PBS, harvested, and treated like CHO cells except that for solubilization of neurons radioimmune precipitation assay buffer was used (1% Nonidet P-40, 0.5% sodium deoxycholate, 0.1% SDS in 50 m m Tris-HCl (pH 7.5), 150 m m NaCl, 1 m m EDTA) supplemented with 1/100 volume of protease inhibitor mixture (Nacalai Tesque) and 1 m m phenylmethylsulfonyl fluoride. .. For the detection of native Kv12.2, crude membrane fractions were prepared from freshly dissected whole brains of mice of different ages (embryonic 17 day, postnatal day 5 (PD5), P13, or adult (P210)).

Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
Article Snippet: .. Cells were lysed in 0.5 ml of TNE lysis buffer containing 150 m m Tris-HCl (pH 7.6), 50 m m NaCl, 1 m m EDTA, and 0.5% Nonidet P-40 supplemented with protease inhibitor mixture (Nacalai Tesque) ( ). .. After lysis, cellular debris was removed by centrifugation at 15,000 × g for 10 min.

Article Title: MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity
Article Snippet: .. Mouse hippocampi were homogenized on ice in 200–400 μl of lysis buffer containing 50 m m Tris-HCl, pH 8.0, 150 m m NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor mixture (Nacalai tesque, Kyoto, Japan) using Potter-type homogenizer. .. Mouse hippocampi were homogenized on ice in 200–400 μl of lysis buffer containing 50 m m Tris-HCl, pH 8.0, 150 m m NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor mixture (Nacalai tesque, Kyoto, Japan) using Potter-type homogenizer.

Purification:

Article Title: Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway I of pyridoxine
Article Snippet: .. The precipitate was dissolved in 1.0 ml 50 m M Tris–HCl pH 7.0 containing 0.1%( v / v ) 2-­mercaptoethanol and the enzyme solution was applied onto a HPLC gel-filtration column (5-diol-300II; Nacalai Tesque, Kyoto, Japan) equilibrated with buffer B as an additional purification step. .. The purified enzyme was dialyzed against 10 m M HEPES pH 7.0 containing 0.1%( v / v ) 2-­mercaptoethanol and concentrated to 14 mg ml−1 with an Amicon Ultra-4 30 kDa Ultracel (Millipore, Billerica, Massachusetts, USA) for crystallization.

Lysis:

Article Title: Interaction between a Domain of the Negative Regulator of the Ras-ERK Pathway, SPRED1 Protein, and the GTPase-activating Protein-related Domain of Neurofibromin Is Implicated in Legius Syndrome and Neurofibromatosis Type 1 *
Article Snippet: .. Cells were lysed in 0.5 ml of TNE lysis buffer containing 150 m m Tris-HCl (pH 7.6), 50 m m NaCl, 1 m m EDTA, and 0.5% Nonidet P-40 supplemented with protease inhibitor mixture (Nacalai Tesque) ( ). .. After lysis, cellular debris was removed by centrifugation at 15,000 × g for 10 min.

Article Title: MTH1, an Oxidized Purine Nucleoside Triphosphatase, Suppresses the Accumulation of Oxidative Damage of Nucleic Acids in the Hippocampal Microglia during Kainate-Induced Excitotoxicity
Article Snippet: .. Mouse hippocampi were homogenized on ice in 200–400 μl of lysis buffer containing 50 m m Tris-HCl, pH 8.0, 150 m m NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor mixture (Nacalai tesque, Kyoto, Japan) using Potter-type homogenizer. .. Mouse hippocampi were homogenized on ice in 200–400 μl of lysis buffer containing 50 m m Tris-HCl, pH 8.0, 150 m m NaCl, 1.0% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, and protease inhibitor mixture (Nacalai tesque, Kyoto, Japan) using Potter-type homogenizer.

Mouse Assay:

Article Title: Brain-specific Phgdh Deletion Reveals a Pivotal Role for l-Serine Biosynthesis in Controlling the Level of d-Serine, an N-methyl-d-aspartate Receptor Co-agonist, in Adult Brain *
Article Snippet: .. The cerebral cortex and hippocampus were dissected from adult brain of Floxed and CKO mice (male, 7–11 weeks old) and were then homogenized in a buffer containing 10 m m Tris-HCl (pH 8.0), 8 m urea, 4% CHAPS, protease inhibitor mixture (Nacalai Tesque), and phosphatase inhibitor mixture (Nacalai Tesque). .. Homogenates were centrifuged at 20,000 × g for 10 min to obtain total protein extracts, and concentrations were determined using a Protein Assay Bicinchoninate kit (Nacalai Tesque).

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    Nacalai m tris hcl
    M Tris Hcl, supplied by Nacalai, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m tris hcl/product/Nacalai
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m tris hcl - by Bioz Stars, 2020-09
    92/100 stars
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